14 results on '"Villa-Valverde, P"'
Search Results
2. Corrigendum: Impact of a TAK-1 inhibitor as a single or as an add-on therapy to riociguat on the metabolic reprograming and pulmonary hypertension in the SUGEN5416/hypoxia rat model
- Author
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Daniel Morales-Cano, Jose Luis Izquierdo-García, Bianca Barreira, Sergio Esquivel-Ruiz, Maria Callejo, Rachele Pandolfi, Palmira Villa-Valverde, Ignacio Rodríguez, Angel Cogolludo, Jesus Ruiz-Cabello, Francisco Perez-Vizcaino, and Laura Moreno
- Subjects
pulmonary hypertension ,antiproliferative ,metabolomics ,combination therapy ,right ventricle ,Therapeutics. Pharmacology ,RM1-950 - Published
- 2023
- Full Text
- View/download PDF
3. Impact of a TAK-1 inhibitor as a single or as an add-on therapy to riociguat on the metabolic reprograming and pulmonary hypertension in the SUGEN5416/hypoxia rat model
- Author
-
Daniel Morales-Cano, Jose Luis Izquierdo-García, Bianca Barreira, Sergio Esquivel-Ruiz, Maria Callejo, Rachele Pandolfi, Palmira Villa-Valverde, Ignacio Rodríguez, Angel Cogolludo, Jesus Ruiz-Cabello, Francisco Perez-Vizcaino, and Laura Moreno
- Subjects
pulmonary hypertension ,antiproliferative ,metabolomics ,combination therapy ,right ventricle ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Despite increasing evidence suggesting that pulmonary arterial hypertension (PAH) is a complex disease involving vasoconstriction, thrombosis, inflammation, metabolic dysregulation and vascular proliferation, all the drugs approved for PAH mainly act as vasodilating agents. Since excessive TGF-β signaling is believed to be a critical factor in pulmonary vascular remodeling, we hypothesized that blocking TGFβ-activated kinase 1 (TAK-1), alone or in combination with a vasodilator therapy (i.e., riociguat) could achieve a greater therapeutic benefit.Methods: PAH was induced in male Wistar rats by a single injection of the VEGF receptor antagonist SU5416 (20 mg/kg) followed by exposure to hypoxia (10%O2) for 21 days. Two weeks after SU5416 administration, vehicle, riociguat (3 mg/kg/day), the TAK-1 inhibitor 5Z-7-oxozeaenol (OXO, 3 mg/kg/day), or both drugs combined were administered for 7 days. Metabolic profiling of right ventricle (RV), lung tissues and PA smooth muscle cells (PASMCs) extracts were performed by magnetic resonance spectroscopy, and the differences between groups analyzed by multivariate statistical methods.Results:In vitro, riociguat induced potent vasodilator effects in isolated pulmonary arteries (PA) with negligible antiproliferative effects and metabolic changes in PASMCs. In contrast, 5Z-7-oxozeaenol effectively inhibited the proliferation of PASMCs characterized by a broad metabolic reprogramming but had no acute vasodilator effects. In vivo, treatment with riociguat partially reduced the increase in pulmonary arterial pressure (PAP), RV hypertrophy (RVH), and pulmonary vascular remodeling, attenuated the dysregulation of inosine, glucose, creatine and phosphocholine (PC) in RV and fully abolished the increase in lung IL-1β expression. By contrast, 5Z-7-oxozeaenol significantly reduced pulmonary vascular remodeling and attenuated the metabolic shifts of glucose and PC in RV but had no effects on PAP or RVH. Importantly, combined therapy had an additive effect on pulmonary vascular remodeling and induced a significant metabolic effect over taurine, amino acids, glycolysis, and TCA cycle metabolism via glycine-serine-threonine metabolism. However, it did not improve the effects induced by riociguat alone on pulmonary pressure or RV remodeling. None of the treatments attenuated pulmonary endothelial dysfunction and hyperresponsiveness to serotonin in isolated PA.Conclusion: Our results suggest that inhibition of TAK-1 induces antiproliferative effects and its addition to short-term vasodilator therapy enhances the beneficial effects on pulmonary vascular remodeling and RV metabolic reprogramming in experimental PAH.
- Published
- 2023
- Full Text
- View/download PDF
4. A Contribution to the Harmonization of Non-targeted NMR Methods for Data-Driven Food Authenticity Assessment
- Author
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Gallo, Vito, Ragone, Rosa, Musio, Biagia, Todisco, Stefano, Rizzuti, Antonino, Mastrorilli, Piero, Pontrelli, Stefania, Intini, Nicola, Scapicchio, Pasquale, Triggiani, Maurizio, Pascazio, Antonello, Cobas, Carlos, Mari, Silvia, Garino, Cristiano, Arlorio, Marco, Acquotti, Domenico, Airoldi, Cristina, Arnesano, Fabio, Assfalg, Michael, Barison, Andersson, Benevelli, Francesca, Borioni, Anna, Cagliani, Laura Ruth, Casadei, Luca, Marincola, Flaminia Cesare, Colson, Kim, Consonni, Roberto, Costantino, Gabriele, Cremonini, Mauro Andrea, Davalli, Silvia, Duarte, Iola, Guyader, Sophie, Hamon, Erwann, Hegmanns, Maren, Lamanna, Raffaele, Longobardi, Francesco, Mallamace, Domenico, Mammi, Stefano, Markus, Michelle, Menezes, Leociley Rocha Alencar, Milone, Salvatore, Molero-Vilchez, Dolores, Mucci, Adele, Napoli, Claudia, Rossi, Maria Cecilia, Sáez-Barajas, Elena, Savorani, Francesco, Schievano, Elisabetta, Sciubba, Fabio, Sobolev, Anatoly, Takis, Panteleimon G., Thomas, Freddy, Villa-Valverde, Palmira, and Latronico, Mario
- Published
- 2020
- Full Text
- View/download PDF
5. A dual 1H/19F birdcage coil for small animals at 7 T MRI
- Author
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Villa-Valverde, Palmira, Rodríguez, Ignacio, Padró, Daniel, Benito, Marina, Garrido-Salmon, Carlos Ernesto, and Ruiz-Cabello, Jesús
- Published
- 2019
- Full Text
- View/download PDF
6. Relevance of control diet choice in metabolic studies: impact in glucose homeostasis and vascular function
- Author
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González-Blázquez, Raquel, Alcalá, Martín, Fernández-Alfonso, María S., Villa-Valverde, Palmira, Viana, Marta, Gil-Ortega, Marta, and Somoza, Beatriz
- Published
- 2020
- Full Text
- View/download PDF
7. A dual H-1/F-19 birdcage coil for small animals at 7T MRI
- Author
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Villa-Valverde, P, Rodriguez, I, Padro, D, Benito, M, Garrido-Salmon, CE, and Ruiz-Cabello, J
- Abstract
ObjectiveGiven the growing interest in fluorine, it is necessary to develop new multi-tuned RF coils. Therefore, our objective is to design a simple and versatile double-tuned RF coil that can be used as a transmitter and receiver double-tuned coil (H-1 and F-19) or as transmitter-only double-tuned coil. Materials and methodsA high-pass eight-element birdcage coil was built for H-1 and F-19 for a 7T scanner. PIN diodes and cable traps to block unwanted common mode currents in cables were introduced to confer more flexibility to the coil. S-parameters and quality factor were measured in workbench and signal to noise ratio as well as signal intensity profiles in imaging experiments. ResultsBench measurements show S11 values less than -33dB, S21 lower than -13dB and quality factors ratio of the order of 1.8 that are in agreement with good performances of a RF coil, as well as values of -39dB for F-19 and -30dB for H-1 as good detuning values. Signal intensity profiles prove excellent homogeneity at H-1 and F-19. DiscussionWe present a simple structure of a double-tuned high-pass birdcage coil tuned to H-1 and F-19 that shows a great uniformity and sensitivity for F-19.
- Published
- 2019
8. Fluorine Labeling of Nanoparticles and In Vivo 19F Magnetic Resonance Imaging.
- Author
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Arango, Juan Manuel, Padro, Daniel, Blanco, Jorge, Lopez-Fernandez, Sonia, Castellnou, Pilar, Villa-Valverde, Palmira, Ruiz-Cabello, Jesús, Martin, Abraham, and Carril, Mónica
- Published
- 2021
- Full Text
- View/download PDF
9. Fluorine Labeling of Nanoparticles and In Vivo 19F Magnetic Resonance Imaging
- Author
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Arango, Juan Manuel, Padro, Daniel, Blanco, Jorge, Lopez-Fernandez, Sonia, Castellnou, Pilar, Villa-Valverde, Palmira, Ruiz-Cabello, Jesús, Martin, Abraham, and Carril, Mónica
- Abstract
Fluorinated nanoparticles have increasing applications, but they are still challenging to prepare, especially in the case of water-soluble fluorinated nanoparticles. Herein, a fluorine labeling strategy is presented that is based on the conjugation of custom-made small fluorinated building blocks, obtained by simple synthetic transformations, with carboxylated gold nanoparticles through a convenient phase-transfer process. The synthesis of four fluorinated building blocks with different chemical shifts in 19F nuclear magnetic resonance and varied functionalities is reported, along with their conjugation onto nanoparticles. Fluorinated nanoparticles of small core size obtained by this conjugation methodology and by direct synthesis presented high transverse relaxation times (T2) ranging from 518 to 1030 ms, and a large number of equivalent fluorine atoms per nanoparticle (340–1260 fluorine atoms), which made them potential candidates for 19F magnetic resonance related applications. Finally, nontargeted fluorinated nanoparticles were probed by performing in vivo19F magnetic resonance spectroscopy (19F MRS) in mice. Nanoparticles were detected at both 1 and 2 h after being injected. 19F MRI images were also acquired after either intravenous or subcutaneous injection. Their fate was studied by analyzing the gold content in tissues by ICP-MS. Thus, the present work provides a general fluorination strategy for nanoparticles and shows the potential use of small fluorinated nanoparticles in magnetic-resonance-related applications.
- Published
- 2021
- Full Text
- View/download PDF
10. A Contribution to the Harmonization of Non-targeted NMR Methods for Data-Driven Food Authenticity Assessment
- Author
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Piero Mastrorilli, Michael Assfalg, Vito Gallo, Rosa Ragone, Palmira Villa-Valverde, Iola F. Duarte, Maurizio Triggiani, Anna Borioni, Biagia Musio, Antonello Pascazio, Francesco Longobardi, Silvia Davalli, Elisabetta Schievano, Silvia Mari, Stefania Pontrelli, Cristiano Garino, Cristina Airoldi, Carlos Cobas, Flaminia Cesare Marincola, Adele Mucci, Andersson Barison, Antonino Rizzuti, Erwann Hamon, Michelle Markus, Panteleimon G. Takis, Francesco Savorani, Luca Casadei, Freddy Thomas, Fabio Sciubba, Domenico Acquotti, Anatoly P. Sobolev, Claudia Di Napoli, Kim Colson, Elena Sáez-Barajas, Francesca Benevelli, Nicola Intini, Gabriele Costantino, Leociley R. A. Menezes, Marco Arlorio, Domenico Mallamace, Mauro Andrea Cremonini, Pasquale Scapicchio, Raffaele Lamanna, Sophie Guyader, Stefano Todisco, Maria Cecilia Rossi, Mario Latronico, Laura Ruth Cagliani, Maren Hegmanns, Fabio Arnesano, Dolores Molero-Vílchez, Stefano Mammi, Roberto Consonni, Salvatore Milone, Gallo, V, Ragone, R, Musio, B, Todisco, S, Rizzuti, A, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Pascazio, A, Cobas, C, Mari, S, Garino, C, Arlorio, M, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Barison, A, Benevelli, F, Borioni, A, Cagliani, L, Casadei, L, Marincola, F, Colson, K, Consonni, R, Costantino, G, Cremonini, M, Davalli, S, Duarte, I, Guyader, S, Hamon, E, Hegmanns, M, Lamanna, R, Longobardi, F, Mallamace, D, Mammi, S, Markus, M, Menezes, L, Milone, S, Molero-Vilchez, D, Mucci, A, Napoli, C, Rossi, M, Saez-Barajas, E, Savorani, F, Schievano, E, Sciubba, F, Sobolev, A, Takis, P, Thomas, F, Villa-Valverde, P, Latronico, M, Gallo, V., Ragone, R., Musio, B., Todisco, S., Rizzuti, A., Mastrorilli, P., Pontrelli, S., Intini, N., Scapicchio, P., Triggiani, M., Pascazio, A., Cobas, C., Mari, S., Garino, C., Arlorio, M., Acquotti, D., Airoldi, C., Arnesano, F., Assfalg, M., Barison, A., Benevelli, F., Borioni, A., Cagliani, L. R., Casadei, L., Marincola, F. C., Colson, K., Consonni, R., Costantino, G., Cremonini, M. A., Davalli, S., Duarte, I., Guyader, S., Hamon, E., Hegmanns, M., Lamanna, R., Longobardi, F., Mallamace, D., Mammi, S., Markus, M., Menezes, L. R. A., Milone, S., Molero-Vilchez, D., Mucci, A., Napoli, C., Rossi, M. C., Saez-Barajas, E., Savorani, F., Schievano, E., Sciubba, F., Sobolev, A., Takis, P. G., Thomas, F., Villa-Valverde, P., and Latronico, M.
- Subjects
Non targeted ,Future studies ,Inter-laboratory comparison ,Computer science ,Food authenticity ,Food fingerprinting ,Non-targeted analysis ,NMR ,Validation ,Human error ,computer.software_genre ,01 natural sciences ,Applied Microbiology and Biotechnology ,Analytical Chemistry ,Data-driven ,0404 agricultural biotechnology ,non targeted analysis ,inter laboratory comparison ,Safety, Risk, Reliability and Quality ,Equivalence (measure theory) ,Non-targeted analysis .NMR ,2. Zero hunger ,010401 analytical chemistry ,04 agricultural and veterinary sciences ,Nuclear magnetic resonance spectroscopy ,Non-targeted analysi ,040401 food science ,0104 chemical sciences ,Identification (information) ,Food products ,Data mining ,Safety Research ,computer ,Food Science - Abstract
Spectroscopic non-targeted methods are gaining ever-growing importance in quality control and authenticity assessment of food products because of their strong potential for identification of specific features of the products by data-driven classifiers. One of the factors hampering the diffusion of spectroscopic non-targeted methods and data-driven classifiers is the lack of harmonized guidelines for their development and validation. In particular, to date, neither conditions to directly compare spectra recorded by different spectrometers nor studies demonstrating the statistical equivalence of the spectra are available. Among the spectroscopic analytical techniques suitable for the development of non-targeted methods, nuclear magnetic resonance (NMR) offers the unique opportunity to generate statistically equivalent signals. In this paper, the feasibility of NMR spectroscopy to generate statistically equivalent NMR signals from a number of different spectrometers was demonstrated for complex mixtures (aqueous extracts of wheat and flour) by organizing an inter-laboratory comparison involving 36 NMR spectrometers. Univariate statistics along with multivariate analysis were exploited to establish unbiased criteria for assessing the statistical equivalence of the NMR signals. The aspects affecting the signal equivalence were investigated, and possible solutions to reduce the extent of the human error were proposed and applied with satisfactory results. This study furnishes the scientific community with an appropriate and easy procedure to validate non-targeted NMR methods and provides error values to be used as a reference for future studies.
- Published
- 2019
11. Corrigendum: Impact of a TAK-1 inhibitor as a single or as an add-on therapy to riociguat on the metabolic reprograming and pulmonary hypertension in the SUGEN5416/hypoxia rat model.
- Author
-
Morales-Cano D, Izquierdo-García JL, Barreira B, Esquivel-Ruiz S, Callejo M, Pandolfi R, Villa-Valverde P, Rodríguez I, Cogolludo A, Ruiz-Cabello J, Perez-Vizcaino F, and Moreno L
- Abstract
[This corrects the article DOI: 10.3389/fphar.2023.1021535.]., (Copyright © 2023 Morales-Cano, Izquierdo-García, Barreira, Esquivel-Ruiz, Callejo, Pandolfi, Villa-Valverde, Rodríguez, Cogolludo, Ruiz-Cabello, Perez-Vizcaino and Moreno.)
- Published
- 2023
- Full Text
- View/download PDF
12. Impact of a TAK-1 inhibitor as a single or as an add-on therapy to riociguat on the metabolic reprograming and pulmonary hypertension in the SUGEN5416/hypoxia rat model.
- Author
-
Morales-Cano D, Izquierdo-García JL, Barreira B, Esquivel-Ruiz S, Callejo M, Pandolfi R, Villa-Valverde P, Rodríguez I, Cogolludo A, Ruiz-Cabello J, Perez-Vizcaino F, and Moreno L
- Abstract
Background: Despite increasing evidence suggesting that pulmonary arterial hypertension (PAH) is a complex disease involving vasoconstriction, thrombosis, inflammation, metabolic dysregulation and vascular proliferation, all the drugs approved for PAH mainly act as vasodilating agents. Since excessive TGF-β signaling is believed to be a critical factor in pulmonary vascular remodeling, we hypothesized that blocking TGFβ-activated kinase 1 (TAK-1), alone or in combination with a vasodilator therapy (i.e., riociguat) could achieve a greater therapeutic benefit. Methods: PAH was induced in male Wistar rats by a single injection of the VEGF receptor antagonist SU5416 (20 mg/kg) followed by exposure to hypoxia (10%O
2 ) for 21 days. Two weeks after SU5416 administration, vehicle, riociguat (3 mg/kg/day), the TAK-1 inhibitor 5Z-7-oxozeaenol (OXO, 3 mg/kg/day), or both drugs combined were administered for 7 days. Metabolic profiling of right ventricle (RV), lung tissues and PA smooth muscle cells (PASMCs) extracts were performed by magnetic resonance spectroscopy, and the differences between groups analyzed by multivariate statistical methods. Results: In vitro , riociguat induced potent vasodilator effects in isolated pulmonary arteries (PA) with negligible antiproliferative effects and metabolic changes in PASMCs. In contrast, 5Z-7-oxozeaenol effectively inhibited the proliferation of PASMCs characterized by a broad metabolic reprogramming but had no acute vasodilator effects. In vivo, treatment with riociguat partially reduced the increase in pulmonary arterial pressure (PAP), RV hypertrophy (RVH), and pulmonary vascular remodeling, attenuated the dysregulation of inosine, glucose, creatine and phosphocholine (PC) in RV and fully abolished the increase in lung IL-1β expression. By contrast, 5Z-7-oxozeaenol significantly reduced pulmonary vascular remodeling and attenuated the metabolic shifts of glucose and PC in RV but had no effects on PAP or RVH. Importantly, combined therapy had an additive effect on pulmonary vascular remodeling and induced a significant metabolic effect over taurine, amino acids, glycolysis, and TCA cycle metabolism via glycine-serine-threonine metabolism. However, it did not improve the effects induced by riociguat alone on pulmonary pressure or RV remodeling. None of the treatments attenuated pulmonary endothelial dysfunction and hyperresponsiveness to serotonin in isolated PA. Conclusion: Our results suggest that inhibition of TAK-1 induces antiproliferative effects and its addition to short-term vasodilator therapy enhances the beneficial effects on pulmonary vascular remodeling and RV metabolic reprogramming in experimental PAH., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Morales-Cano, Izquierdo-García, Barreira, Esquivel-Ruiz, Callejo, Pandolfi, Villa-Valverde, Rodríguez, Cogolludo, Ruiz-Cabello, Perez-Vizcaino and Moreno.)- Published
- 2023
- Full Text
- View/download PDF
13. Fluorine Labeling of Nanoparticles and In Vivo 19 F Magnetic Resonance Imaging.
- Author
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Arango JM, Padro D, Blanco J, Lopez-Fernandez S, Castellnou P, Villa-Valverde P, Ruiz-Cabello J, Martin A, and Carril M
- Subjects
- Animals, Fluorine pharmacokinetics, Gold pharmacokinetics, Mice, Nanoparticles analysis, Nanoparticles ultrastructure, Tissue Distribution, Fluorine chemistry, Fluorine-19 Magnetic Resonance Imaging methods, Gold chemistry, Nanoparticles chemistry
- Abstract
Fluorinated nanoparticles have increasing applications, but they are still challenging to prepare, especially in the case of water-soluble fluorinated nanoparticles. Herein, a fluorine labeling strategy is presented that is based on the conjugation of custom-made small fluorinated building blocks, obtained by simple synthetic transformations, with carboxylated gold nanoparticles through a convenient phase-transfer process. The synthesis of four fluorinated building blocks with different chemical shifts in
19 F nuclear magnetic resonance and varied functionalities is reported, along with their conjugation onto nanoparticles. Fluorinated nanoparticles of small core size obtained by this conjugation methodology and by direct synthesis presented high transverse relaxation times ( T2 ) ranging from 518 to 1030 ms, and a large number of equivalent fluorine atoms per nanoparticle (340-1260 fluorine atoms), which made them potential candidates for19 F magnetic resonance related applications. Finally, nontargeted fluorinated nanoparticles were probed by performing in vivo19 F magnetic resonance spectroscopy (19 F MRS) in mice. Nanoparticles were detected at both 1 and 2 h after being injected.19 F MRI images were also acquired after either intravenous or subcutaneous injection. Their fate was studied by analyzing the gold content in tissues by ICP-MS. Thus, the present work provides a general fluorination strategy for nanoparticles and shows the potential use of small fluorinated nanoparticles in magnetic-resonance-related applications.- Published
- 2021
- Full Text
- View/download PDF
14. Mild and Short-Term Caloric Restriction Prevents Obesity-Induced Cardiomyopathy in Young Zucker Rats without Changing in Metabolites and Fatty Acids Cardiac Profile.
- Author
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Ruiz-Hurtado G, García-Prieto CF, Pulido-Olmo H, Velasco-Martín JP, Villa-Valverde P, Fernández-Valle ME, Boscá L, Fernández-Velasco M, Regadera J, Somoza B, and Fernández-Alfonso MS
- Abstract
Caloric restriction (CR) ameliorates cardiac dysfunction associated with obesity. However, most of the studies have been performed under severe CR (30-65% caloric intake decrease) for several months or even years in aged animals. Here, we investigated whether mild (20% food intake reduction) and short-term (2-weeks) CR prevented the obese cardiomyopathy phenotype and improved the metabolic profile of young (14 weeks of age) genetically obese Zucker fa/fa rats. Heart weight (HW) and HW/tibia length ratio was significantly lower in fa/fa rats after 2 weeks of CR than in counterparts fed ad libitum . Invasive pressure measurements showed that systolic blood pressure, maximal rate of positive left ventricle (LV) pressure, LV systolic pressure and LV end-diastolic pressure were all significantly higher in obese fa/fa rats than in lean counterparts, which were prevented by CR. Magnetic resonance imaging revealed that the increase in LV end-systolic volume, stroke volume and LV wall thickness observed in fa/fa rats was significantly lower in animals on CR diet. Histological analysis also revealed that CR blocked the significant increase in cardiomyocyte diameter in obese fa/fa rats. High resolution magic angle spinning magnetic resonance spectroscopy analysis of the LV revealed a global decrease in metabolites such as taurine, creatine and phosphocreatine, glutamate, glutamine and glutathione, in obese fa/fa rats, whereas lactate concentration was increased. By contrast, fatty acid concentrations in LV tissue were significantly elevated in obese fa/fa rats. CR failed to restore the LV metabolomic profile of obese fa/fa rats. In conclusion, mild and short-term CR prevented an obesity-induced cardiomyopathy phenotype in young obese fa/fa rats independently of the cardiac metabolic profile.
- Published
- 2017
- Full Text
- View/download PDF
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