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Wildlife trade, both legal and illegal, is increasingly recognized as a key factor in the rise of emerging viral infectious diseases, and this is especially apparent in Asia, where large numbers of wildlife are openly offered for sale in bird markets. We here focus on the risk of Psittacosis becoming a zoonotic disease in the wildlife markets of Java and Bali, Indonesia. Psittacosis is particularly prevalent in parrots (hence the name), and the trade in parrots was instrumental in the Great Parrot Fever Pandemic in 1929/1930. Between 2014 and 2023, we conducted 176 surveys of 14 bird markets, during which we recorded 4446 largely wild-caught parrots for sale. On average, each market had nine genera on offer, and the diversity of genera increased with the increasing presence of parrots (up to 16 genera). For most of the bird markets during each survey, parrots from different genera and originating from different parts of the world, were offered for sale alongside each other. Genera offered for sale together did not cluster into natural (geographic) groups. We found no temporal difference in the sale of parrots. We conclude that the omnipresence of wild-caught parrots from various geographic regions in large numbers within the same bird markets increases the risk that psittacosis is present and that this poses a real risk for the zoonotic spread of avian chlamydiosis to humans.

Zoo collection management is increasingly driven by meeting global conservation needs. Many avian species have experienced population declines throughout Southeast Asia, underscoring the importance of ex situ conservation in these countries. We focus on Thailand, a bird diversity hotspot with a long tradition of keeping birds in captive settings. We aimed to understand what drives species acquisition and maintenance in Thai zoos. To that end, we surveyed 55 zoos, making a complete inventory of reptiles, birds, and mammals on display. We recorded 249 bird species, of which 149 are not native to Thailand. Bird species diversity was positively correlated with mammal species diversity but not with the entry ticket price, the Gross Domestic Product of the province in which the zoo was based, or the size of the zoo. Diversity did differ significantly between zoo types (accredited, government and private zoos). There was a clear difference in the proportion of native and non-native species between zoos, with private zoos containing the highest number of non-native species, which may be related to the licensing status of these zoos. The composition of bird species in Thai zoos appears to be largely driven by their availability, the legal status for keeping them and serendipity. The conservation status seems to be of minor importance, contradicting the typical role of a zoo. To be considered global conservation players, zoos in countries of high species diversity, such as Thailand, have the unique opportunity to provide breeding programmes for some of the rarest species, yet they must improve their collection management plans to focus on such aims.

Humans have long delighted in being entertained by captive wild animals. In zoos and aquariums, animal–visitor interactions allow visitors to experience direct or indirect contact with animals. Well-managed animal–visitor interactions can help zoos and aquariums contribute to their educational objectives by eliciting pro-conservation behaviours from visitors while providing good animal welfare for the animals involved. However, some interactions are dysfunctional because they cause physical and psychological harm to the animal and reinforce negative values and beliefs towards conservation. We aimed to assess how dysfunctional animal–visitor interactions are and whether accreditation by zoological associations reduced the number of such interactions. We visited Thai zoos in person and conducted online monitoring of facilities in Southeast Asia. We report 1165 animal–visitor interactions in nine Southeast Asian countries in 225 zoos and aquariums. Taking photos with animals (“selfies”) and riding on animals were scored as the animal–visitor interactions that were the most dysfunctional. The number of animal–visitor interactions was not less in accredited zoos, and there was little difference among countries. Behaviour change needs to form a strategy for change, in addition to robust legislation and improved efforts by zoological associations to push members to maintain standards and mentor non-accredited facilities.

A growing global human population, habitat conversion, and the indiscriminate exploitation of natural resources have created unsustainable demands on nature, resulting in widespread biodiversity loss. Primates, which represent the third most specious Order of mammals, are facing an extinction crisis. Currently, 69% of primate species are listed by the IUCN as threatened (Vulnerable, Endangered, or Critically Endangered) and 94% have declining populations. Here, we examine two primary threats to primate population persistence, namely the commercialized hunting and capturing of wild primates and their body parts for food, traditional medicine, pets, and use in biomedical research. Both the legal wildlife trade and illegal wildlife trafficking represent multibillion-dollar industries that contribute to primate population decline, a reduction in genetic diversity, and local extirpation. Trade and trafficking also can lead to the emergence of infectious diseases, increasing biosecurity risks to humans. Between 2015 and 2021, CITES reported 337,511 live primates representing at least 99 species were legally traded, with 6.5% sourced directly from the wild. The recent indictment of Cambodian officials for allegedly laundering wild-caught long-tailed macaques into the U.S. by labelling them as captive-bred, highlights the need for greater transparency and accountability. Comprehensive data on the illegal trafficking of primates are extremely difficult to obtain. However, between 2009 and 2017, primates accounted for 20% of all seizures of illegally traded mammals in the air transport sector. International wildlife trafficking is dominated by criminal networks, corruption, and driven by the demands of wealthy consumers. In addition, the internet has expanded international opportunities to connect buyers and sellers of wild-caught primates and their body parts. Despite explicit bans on selling endangered primates, social media sites continue to do so. Moreover, data on the global food security index (GFSI) indicate that as the international demand for wild live primates, their meat, and other body parts has continued to increase, the majority of people in primate range nations have remained food insecure. Given that almost 70% of primate species are negatively impacted by hunting and trapping, we offer a set of recommendations to reduce the trade and trafficking of wild primates.

An animal’s ability to navigate its home range in search of essential resources is a key aspect of its ecology. To reach these resources, animals employ varying navigational processes depending on their exocentric or egocentric view of their environment. The goal of this study was to determine if the Javan slow loris (Nycticebus javanicus), a nocturnal arboreal primate found in southeast Asia, uses some form of cognitive map and spatial memory while navigating their environment. Using behavioural and GPS data of six males and seven females collected at the Little Fireface Project field station based in West Java, Indonesia, we measured their frequency of revisiting important feeding trees, route overlap, and points where individuals significantly changed directions. We found that all individuals predominantly used four tree species while feeding and foraging. The lorises also displayed a high level of route overlap, leading us to conclude that they likely utilize a route-based cognitive map where certain landmarks are integral to their nightly movement. Few studies have specifically focused on strepsirrhine spatial cognition in the wild; here, we show the navigational mechanism used by the Javan slow loris to reach distant/out-of-sight resources. The evident reliance on spatial cognition in a strepsirrhine species suggest that it could be an important selective pressure for primates at the earliest stages of primate cognitive evolution. In addition to the importance of spatial memory in theoretical discourse, understanding slow loris movement has practical applications to conservation, particularly regarding the numerous translocations undertaken by individuals rescued from the illegal wildlife trade. We discuss the importance of considering soft release training and monitoring in such releases.

Abstract Conservation funding is currently limited; cost‐effective conservation solutions are essential. We suggest that the thousands of field stations worldwide can play key roles at the frontline of biodiversity conservation and have high intrinsic value. We assessed field stations’ conservation return on investment and explored the impact of COVID‐19. We surveyed leaders of field stations across tropical regions that host primate research; 157 field stations in 56 countries responded. Respondents reported improved habitat quality and reduced hunting rates at over 80% of field stations and lower operational costs per km2 than protected areas, yet half of those surveyed have less funding now than in 2019. Spatial analyses support field station presence as reducing deforestation. These “earth observatories” provide a high return on investment; we advocate for increased support of field station programs and for governments to support their vital conservation efforts by investing accordingly.

The intensive management of cropland refers to a reduction in habitat complexity (i.e., shade tree cover, tree species richness, crop species richness) to gain more profits. This usually entails a decrease in biodiversity, but agroforestry systems have been shown to provide a solution to the need for profits while maintaining biodiversity and ecosystem services. Invertebrates are important bioindicators since they are not just affected by a decrease in habitat complexity; they are also key for the maintenance of ecosystems given their ecological roles. We aimed to understand how agricultural intensification impacted invertebrate abundance and richness in an agroforestry system in Bali, Indonesia. We set up 53 × 25 m2 plots and collected data via pitfall and pan traps. We linked those data to vegetation data (canopy cover, tree species richness, crop species richness), habitat type (rustic vs. polyculture), and productivity. Overall, we found that the abundance and richness of invertebrate taxa were positively influenced by increasing canopy cover and crop and tree species richness. This supports the habitat heterogeneity hypothesis, which indicates that increased habitat complexity promotes higher invertebrate species richness and abundance. The abundance and richness of certain invertebrate taxa, including agents of biocontrol, were shown to increase in plots with higher yields, thus solidifying the important role of invertebrate communities in the provision of ecosystem services. Harvesting crops from complex agroforestry systems ensures a sustainable income for local communities as well as habitats for invertebrates.

Agricultural expansion has had a detrimental effect on tropical forests and the animal communities that depend on them. Agroforestry systems, however, with their more complex tree and plant communities, have been shown to be important habitats for a range of globally threatened species, including nocturnal animals. Here, we present novel data on the encounter rates of seven species of nocturnal mammals in relation to agroforestry systems within four national parks and associated plantations in Bangladesh to examine if encounter rates were influenced by the human population density, presence of plantations, and human access as represented by a Human Influence Index of anthropogenic disturbance. We walked 70.3 km of transects with only semi-natural forest, 26.9 km of transects with semi-natural forest and gardens, and 21.7 km of transects with semi-natural forest and monocultures over 55 nights from 2017–2019. Of the seven species of nocturnal mammals we detected, all were present in Satachari National Park, whereas six occurred in Lawachara National Park, Rajkandi Forest Range, and Rema-Kalenga Wildlife Sanctuary. Within these national parks, three species (Bengal slow loris, large Indian civet, particolored flying squirrel) were more frequently recorded in areas with human disturbance, especially agroforestry plantations. With declining forest cover in Bangladesh, we highlight here the potential of agroforestry systems as emerging important habitats for these species. We encourage long-term studies of these lesser-studied taxa to understand fully the capacity of agroforestry systems in order to support their long-term conservation.

Vector-borne diseases spread from wild animals and their associated ectoparasites to humans and domesticated animals. Wildlife markets are recognized as important areas where this transfer can take place. We assessed the potential for spreading vector-borne diseases in two live and wet markets in Myanmar (Mong La, on the Myanmar-China border) and Indonesia (Sukahaji in Bandung on the island of Java) by making an inventory of all live and freshly killed wild mammals for sale. For eight mammal families, we quantified the number of animals on offer, and we used a heatmap cluster analysis to map vector-borne diseases that these families may carry. In Myanmar, we observed large numbers of wild pigs and deer (potentially carrying West Nile and various encephalitis viruses) whereas in Indonesia we observed Old World fruit bats (potentially carrying Chikungunya and encephalitis viruses) and squirrels (potentially carrying West Nile and encephalitis viruses). The trade in Indonesia was dominated by live mammals offered for sale as pets, and only Old World fruit bats and squirrels traded for traditional Asian medicine were killed in the markets. The trade in Myanmar was more geared towards wild meat (e.g., wild pigs, deer, primates) and traditional Asian medicine (squirrels). The combined risks of vector-borne diseases spreading from traded animals to human health highlight the need for an integrated approach protecting public health, economic interests and biodiversity.

Short-term memory is implicated in a range of cognitive abilities and is critical for understanding primate cognitive evolution. To investigate the effects of phylogeny, ecology and sociality on short-term memory, we tested the largest and most diverse primate sample to date (421 non-human primates across 41 species) in an experimental delayed-response task. Our results confirm previous findings that longer delays decrease memory performance across species and taxa. Our analyses demonstrate a considerable contribution of phylogeny over ecological and social factors on the distribution of short-term memory performance in primates; closely related species had more similar short-term memory abilities. Overall, individuals in the branch of Hominoidea performed better compared to Cercopithecoidea, who in turn performed above Platyrrhini and Strepsirrhini. Interdependencies between phylogeny and socioecology of a given species presented an obstacle to disentangling the effects of each of these factors on the evolution of short-term memory capacity. However, this study offers an important step forward in understanding the interspecies and individual variation in short-term memory ability by providing the first phylogenetic reconstruction of this trait’s evolutionary history. The dataset constitutes a unique resource for studying the evolution of primate cognition and the role of short-term memory in other cognitive abilities.

Abstract Trade of wildlife for use in traditional medicines, rituals, magical spells and cultural practices occurs globally and has been studied mostly in Africa and Asia. The grey slender loris Loris lydekkerianus is used for both medicinal and ritual purposes, but little information is available on how the user is meant to extract their medicinal properties, or the potential impact these practices have on the species' populations. From 2014–2021, we used open‐ended interviews with 293 informants in three slender loris range states in Southern India to collect qualitative information on people's beliefs regarding the use of slender lorises in traditional medicine, black magic rituals and other cultural practices. To understand this further, we analysed data on 139 live slender loris rescues from three rescue and rehabilitation centres and one government organization in Bengaluru, India collected over an 18‐year period. We found that 116/139 live individuals had been involved in black magic rituals, including piercing, or burning the body and the eyes. These ritual practices occurred more often to female slender lorises and during the new moon. Data from 293 interviews revealed that astrologers regularly use live lorises for fortune‐telling or for warding off evil. Slender loris body parts are used to make traditional folk medicine, develop black magic potions that bring people harm, hypnotize people or to thwart evil. Habitat loss and anthropogenic pressures, coupled with the existing slender loris trade for cultural practices, are a cause for grave concern. Numerous deep‐rooted superstitious beliefs and rituals continue to thrive in modern India, and this is potentially one of the major threats to India's already imperilled slender loris population. More research into the prevalence of loris use for black magic is needed to assess the impact on species sustainability. Read the free Plain Language Summary for this article on the Journal blog.

Assessing the sustainability of the harvest of animals can be done by obtaining data from processing facilities and establishing that vital attributes of the harvested animals (e.g., size, age structure, sex ratio) do not change over time. This model works if the traders operate in a free market without any regulations on what can be harvested, processed or exported, and when harvest methods and harvest areas do not change between assessment periods. Several studies assessed the harvest effects on blood pythons (Python brongersmai) in North Sumatra, Indonesia seemingly under a free market scenario, with some concluding that trade was sustainable and the others hinting at an overharvest. Indonesia has established harvest and export quotas and, internationally, trade in blood pythons is regulated through CITES, and the blood python trade clearly does not operate in a free market. Data suggest that the three (or four) slaughterhouses included in these studies processed ~27,000 blood pythons a year against a quota of 18,000. There is a risk that data from traders alone purporting to show that harvest is sustainable will lead to an increase of quotas or an abandonment of quotas altogether. There is no conclusive data to support that the harvest of blood pythons in North Sumatra is sustainable but there is sufficient evidence to suggest that a substantial part of this trade is illegal. Likewise, at a global level there are clear indications of misdeclared, underreported and illegal trade involving 10,000 s of blood pythons. While important biological information can be obtained from harvested animals, to assess whether harvest is sustainable there is no substitute for monitoring wild populations. After decades of international trade in blood pythons from Indonesia, during which at least half a million blood pythons were exported, it is all the more urgent that systematic monitoring of wild populations commences.

Lorisiformes are nocturnal primates from Africa and Asia with four genera, with two (Arctocebus and Loris), three (Perodicticus) and nine (Nycticebus) recognised species. Their cryptic lifestyle and lack of study have resulted in an underappreciation of the variation at the species and genus level. There are marked differences between the pygmy slow loris Nycticebus pygmaeus and the other Nycticebus species and, in the past, several authors have suggested that these may warrant recognition at the generic level. We here combine morphological, behavioural, karyotypical and genetic data to show that these contrasts are, indeed, significantly large and consistent. We propose Xanthonycticebus gen. nov. as a new genus name for the pygmy slow lorises and suggest a common name of pygmy lorises. Based on analysis of complete mitochondrial DNA sequences, we calculate the divergence of pygmy from slow lorises at 9.9–10.0%. The median date, calculated for the divergence between Xanthonycticebus and Nycticebus, is 10.5 Mya (range 4.9–21.0 Mya). Xanthonycticebus differs from Nycticebus by showing sympatry with other slow loris species, by habitually giving birth to twins, by showing seasonal body mass and whole body coat colour changes (absent in other species living at similar latitudes) and a multi-male, multi-female social system. Pygmy lorises are easily recognisable by the absence of hair on their ears and more protruding premaxilla. Xanthonycticebus is threatened by habitat loss and illegal trade despite legal protection across their range and all slow lorises are listed on appendix 1 of CITES. The suggested nomenclatural changes should not affect their legal status.

Monitoring illegal wildlife trade and how the modus operandi of traders changes over time is of vital importance to mitigate the negative effects this trade can have on wild populations. We focused on the trade of birds of prey in Indonesia (2016–2021) in bird markets (12 markets, 194 visits), on Instagram (19 seller profiles) and on Facebook (11 open groups). We link species prevalence and asking prices to body size, abundance and geographic range. Smaller species were more traded in bird markets and less so online. Abundance in trade is in part linked to their abundance in the wild. Asking prices (mean of USD 87) are positively correlated with size and negatively with their abundance in the wild. Authorities seize birds of prey according to their observed abundance in trade, but only 10% of seizures lead to successful prosecutions. The trade is in violation of national laws and the terms and conditions of the online platforms; the low prosecution rate with minimal fines shows a lack of recognition of the urgency of the threat that trade poses to already imperilled wildlife. The shift of trade from physical bird markets to the online marketplace necessitates a different strategy both for monitoring and enforcement.

Authentication of seafood products by means of molecular techniques has relevance for food sustainability and security, as well as international trade regulation, linked to transparency in food manufacturing. We focus on the molecular detection of the depleted European eel Anguilla anguilla, a species for which strict international trade regulations are in place since 2010, in studies conducted outside Europe. We found thirteen studies from nine countries (Canada, China, Japan, Malaysia, Peru, Singapore, South Korea, Taiwan, and USA) for which, on average, 59 ± 28% of the 330 sequenced eel samples comprised European eel. Only China, Japan, South Korea, and USA reported the import of European eel in the years prior to sampling. The authentication of eel products demonstrates a global, in part illegal, trade in European eel, covered up by incomplete or fraudulent labelling. This calls into question the compliance with existing national and international trade regulations and its implications for food safety and sustainability.

We conducted research to understand online trade in jaguar parts and develop tools of utility for jaguars and other species. Our research took place to identify potential trade across 31 online platforms in Spanish, Portuguese, English, Dutch, French, Chinese, and Vietnamese. We identified 230 posts from between 2009 and 2019. We screened the images of animal parts shown in search results to verify if from jaguar; 71 posts on 12 different platforms in four languages were accompanied by images identified as definitely jaguar, including a total of 125 jaguar parts (50.7% posts in Spanish, 25.4% Portuguese, 22.5% Chinese and 1.4% French). Search effort varied among languages due to staff availability. Standardizing for effort across languages by dividing number of posts advertising jaguars by search time and number of individual searches completed via term/platform combinations changed the proportions the rankings of posts adjusted for effort were led by Portuguese, Chinese, and Spanish. Teeth were the most common part; 156 posts offered at least 367 teeth and from these, 95 were assessed as definitely jaguar; 71 of which could be linked to a location, with the majority offered for sale from Mexico, China, Bolivia, and Brazil (26.8, 25.4, 16.9, and 12.7% respectively). The second most traded item, skins and derivative items were only identified from Latin America: Brazil (7), followed by Peru (6), Bolivia (3), Mexico (2 and 1 skin piece), and Nicaragua and Venezuela (1 each). Whether by number of posts or pieces, the most commonly parts were: teeth, skins/pieces of skins, heads, and bodies. Our research took place within a longer-term project to assist law enforcement in host countries to better identify potential illegal trade and presents a snapshot of online jaguar trade and methods that also may have utility for many species traded online.

Illegal, unreported, and unregulated fishing, including that of sharks, poses a significant threat to marine ecosystems and individual species. I use data from the media, tourists, and artisan fishermen to gain insight into the trade in the world’s largest fish, the whale shark (Rhincodon typus). I focus on the Indonesian island of Java where, along its south coast, whale sharks are landed and butchered on the beach in view of hundreds of people and local media. Whale sharks are typically caught in fishing nets and dragged alongside boats to the shallows, where they are butchered. The meat and oil (valued at ~USD 2000 per shark) are sold and distributed within the community. I document 58 landings of mainly immature whale sharks (2002–2022). Artisanal fishermen see the landing of whale sharks as a fortuitous event, but the species is protected, and Indonesia is a signatory to various international agreements that preclude the fishing of whale sharks. It is imperative for the conservation of whale sharks that the various parties in Indonesia adhere better to their own rules and regulations protecting this species.

The Asian Songbird Crisis—the recognition that the cage bird trade is the number one threat to many songbirds—has focussed on either individual bird markets or specific taxa. We here show, through sustained monitoring, that even species that were not thought to be traded are also at risk from overexploitation. The Javan crocias Laniellus albonotatus is endemic to the montane forests of western Java–this remoteness was thought to be the reason why it had escaped from being at risk. We surveyed 21 bird markets over a 44-month period; the number of crocias recorded in markets (1.29 birds/survey) was negatively related to the distance to its habitat. The size of the bird market had no effect on the number of crocias we recorded. Prices (US$26.70/bird), when corrected for inflation, did not change over 12 years suggesting supply can keep up with demand. Turnover is high (50% sold after 8 days), and we estimate the total trade at 1,200 to 1,500 birds/year. Legal protection of Javan crocias seen in isolation thus far is proven to be ineffective. A more inclusive approach where government bodies, conservation agencies and society participate, is needed to curb the trade in this and other imperiled songbirds.

The illegal wildlife trade has direct relevance for zoo management, animal acquisition and disposition and it has no place in modern zoo management. Zoos must not only act within the law of the country in which it is based, but they should also follow the rules and intentions of international trade regulations and, where relevant, domestic laws of the animal’s country of origin. After its rediscovery in 2012, zoos in Asia and Europe started displaying Bornean earless monitor lizards (Lanthanotus borneensis), the ‘Holy Grail of Herpetology’. Earless monitor lizards have been legally protected in each of its three range countries for over four decades and, over this period, no specimen has ever been legally exported. However, the illicit trade in the species is thriving and individuals become more affordable. Using publicly available data, I present a timeline of how and from where a total of 16 zoos acquired their earless monitor lizards, including from private individuals and non-accredited zoos. Apart from one zoo in Japan (since 2012) and one zoo in the USA (since 2021), all non-range country zoos that currently display the species are based in Europe. Their absence prior to 2021 in US zoos (despite an increasing illegal trade) could be explained as the acquisition of earless monitor lizards would have been in violation of the Lacey Act (1900) that requires buyers to ensure that imported or purchased wildlife has not been taken in violation of any foreign law. While there is no evidence that any of the zoos, their directors or their staff have broken any laws – no-one in the zoo community has been convicted for illegally trading earless monitor lizards – with more zoos speaking out against the illegal wildlife trade, it is imperative that zoos behave in an exemplary manner and set high standards. At present, some zoos do not meet this standard.

Abstract Mountain gorilla Gorilla beringei beringei trekking is a substantial source of revenue for the conservation of this threatened primate and its habitat. Trekking, however, may pose a threat of human‐to‐gorilla disease transmission that could have disastrous effects on wild gorillas. We used 858 photographs posted on Instagram in 2013–2019 to analyse the proximity of tourists visiting mountain gorillas in the wild. We classified photographs of the encounters according to the distance between the closest gorilla and human, the age class of the gorilla, the trekking location and presence of a surgical face mask on the tourist. We ran a generalised linear mixed model to test whether these variables influenced the distance between the human and the wild gorillas in the photographs, and to test whether these distances have changed over time. Most sampled photographs (86%) showed tourists within a critical 4 m of the gorillas, with 25 incidents of physical contact between a tourist and a gorilla, and only 3% at the recommended distance of 7 m or more. We only were able to record face mask use in the Democratic Republic of Congo, where these were present in 65% of uploaded photos. Tourists and immature gorillas tended to get closer to each other than tourists and adult gorillas, and this is more pronounced in female tourists than male tourists. The mean distance between human and wild gorillas decreased by ~1 m between 2013 and 2019. The results indicate that existing rules are not enforced and raise attention to this unsustainable aspect of mountain gorilla trekking as it is practiced today. These ever‐growing tourist attractions in the range countries pose risks of disease transmission in both directions between tourists and wildlife. The popularity of photograph‐based social media may stimulate closer contacts and influence people into risky behaviours. We advocate the establishment and reinforcement of regulations relating to the distance between animals and tourists in any in situ wildlife ecotourism context, as well as campaigns to raise awareness regarding the risks of anthroponosis, and fines applied in case of non‐compliance. A free Plain Language Summary can be found within the Supporting Information of this article.

As road infrastructure networks rapidly expand globally, especially in the tropics, previously continuous habitats are being fragmented, resulting in more frequent wildlife–vehicle collisions (WVC). Primates are widespread throughout many sub-/tropical countries, and as their habitats are fragmented, they are increasingly at risk of WVC. We created the Global Primate Roadkill Database (GPRD), the largest available standardized database of primate roadkill incidents. We obtained data from published papers, un-published and citizen science databases, anecdotal reports, news reports, and social media posts. Here, we describe the collection methods for the GPRD and present the most up-to-date version of the database in full. For each primate roadkill incident, we recorded the species killed, the exact location, and the year and month the roadkill was observed. At the time of publication, the GPRD includes 2862 individual primate roadkill records from 41 countries. As primates range in more than twice as many countries, the absence of data from these countries is not necessarily indicative of a lack of primate vehicular collisions. Given the value of these data for addressing both local and global research questions, we encourage conservationists and citizen scientists to contribute to the GPRD so that, together, we can better understand the impact road infrastructure has on primates and evaluate measures which may help mitigate risk-prone areas or species.

Increasingly, new species are being described without there being a name-bearing type specimen. In 2015, a new species of primate was described, the golden-crowned langur Presbytis johnaspinalli Nardelli, 2015 on the basis of five photographs that were posted on the Internet in 2009. After publication, the validity of the species was questioned as it was suggested that the animals were partially and selectively bleached ebony langurs Trachypithecus auratus (É. Geoffroy Saint-Hilaire, 1812). Since the whereabouts of the animals were unknown, it was difficult to see how this matter could be resolved and the current taxonomic status of P. johnaspinalli remains unclear. I present new information about the fate of the individual animals in the photographs and their species identification. In 2009, thirteen of the langurs on which Nardelli based his description were brought to a rescue centre where, after about three months, they regained their normal black colouration confirming the bleaching hypothesis. Eight of the langurs were released in a forest and two were monitored for two months in 2014. The description of their behaviour, photographs and analysis of their cytochrome b genes confirms them as ebony langurs. There is no evidence to support the notion that the golden-crowned langur represents intermediates between melanistic and erythristic ebony langurs, nor that it represents a new species. As such, Presbytis johnaspinalli Nardelli, 2015 is a junior synonym of Trachyptihecus auratus (É. Geoffroy Saint-Hilaire, 1812). This case underscores the importance of assembling a sufficiently varied amount of data prior to describing new species and studying the actual type specimens.

The illegal wildlife trade is considered one of the major threats to global biodiversity. Asian elephants (Elephas maximus) have been highly valued by various cultures for use in religious and spiritual contexts, as a draft animal, and more recently, as a tourist attraction. Thus, the demand for captive elephants is high. Wild Asian elephants are taken from the wild, often illegally, to maintain these captive populations due to the unviability of captive breeding programs. For the first time, we documented the extent to which wild elephants are being illegally captured and traded in Sri Lanka between January 2008 and December 2018. We collected data from case records maintained by the Sri Lanka court system where the suspects of illegal elephant trade were prosecuted in addition to information gathered by archives and interviews with various stakeholders. We documented 55 cases where elephants were illegally traded. This is probably an underestimate due to the mortality rate of elephants during capture operations, and challenges in collecting data on this highly organized illicit trade. Nearly equal numbers of male and female elephants were traded and more than 50% of them were juveniles, aged ≤5 years. Significantly more elephants were found to be seized in 2014–2015 than in the other time periods combined. We found evidence of the illegal capture of wild elephants from wildlife protected areas and state forests. More importantly, we identified evidence of corruption of wildlife officers, involvement of politicians and other high-ranking personnel in the illegal wildlife trade, and lack of active enforcement of wildlife law as major challenges to overcome if the illegal capture and domestic trade of wild elephants in Sri Lanka are to be halted. Based on our study, we make a series of recommendations that should result in implementing policy to reduce the trafficking of Asian elephants in Sri Lanka and improve the conservation management of the species.

The shift of wildlife trade from brick-and-mortar markets to virtual online marketplaces is reported using the trade of birds of prey (raptors) in Thailand from 1968 to 2019 as a case study. We analyzed data obtained from physical market survey data for 2,782 individuals from 27 species, with 2,420 individuals reported in early surveys (1968-1988) and 362 individuals reported in later surveys (2003-2015) and online surveys (February 2017 to January 2019). We compared information on asking prices and what species were traded using two comparative approaches to analyze how species composition may have changed over time (physical markets and online platforms; pre-Internet and Internet era). In comparison with the five previous market surveys, we did not find a statistically significant difference between species and availability of species offered for sale when comparing physical bird markets and online markets. In all data sets, biological factors such as wingspan were significant factors in explaining price variation. We conclude that sustained monitoring is needed to make direct comparisons between the trade platforms. With a continued increase of wildlife trade on online platforms, we recommend increased regulation and enforcement of wildlife trade laws.

Abstract Background Research of many mammal species tends to focus on single habitats, reducing knowledge of ecological flexibility. The Javan lutung (Trachypithecus auratus) is considered a strict forest primate, and little is known about populations living in savannah. In 2017–2018, we investigated the density and distribution of Javan lutung in Baluran National Park, Indonesia. We conducted ad libitum follows and line transect distance sampling with habitat suitability analysis of Javan lutung. Results Estimated density was 14.91 individuals km− 2 (95% CI 7.91–28.08), and estimated population size was 3727 individuals (95% CI 1979 – 7019). Long-tailed macaque (Macaca fascicularis) habitat suitability was the main driver of lutung habitat suitability as the probability of lutung occurrence increased greatly with macaque habitat suitability. Distance to roads, and distance to secondary forest had a negative relationship with lutung occurrence. Lutung habitat suitability decreased with increasing elevation, however, Mt Baluran and the primary forest on Mt Baluran was under-sampled due to treacherous conditions. Follows of six focus groups revealed considerable use of savannah, with terrestrial travel. The follows also revealed polyspecific associations with long-tailed macaques through shared sleeping sites and inter-specific vocalisations. Conclusions Our study provides new knowledge on the general ecology of Javan lutung, such as use of savannah habitats, underlining our need to branch out in our study sites to understand the flexibility and adaptability of our study species. Another undocumented behaviour is the polyspecific association with long-tailed macaques. We encourage more research on this subject.

Non-human primates (primates) are regarded as key research subjects for pre-clinical trials of several drugs aimed to alleviate human suffering. It has long been suggested that the predominant species in the international trade in live primates for use in research is the long-tailed macaque (Macaca fascicularis). However, little is still known about the value of this international trade. Whilst the international trade to supply the requirement for biomedical testing is known to encourage illegal wildlife trade, we lack a detailed understanding of the overall value and magnitude of this trade. Such information is vital to facilitate the design of effective conservation strategies in range countries, in order to mitigate the exploitation of wild populations by organized crime networks. Here, data from CITES and the UN Comtrade databases were combined to calculate the value of this trade. We also compared the number of individual primates traded as reported in the two databases to investigate possible correlations. Results show that, from 2010 to 2019, the international trade in long-tailed macaques constituted a market worth of ~US $1.25 billion. We found a positive correlation between individual primates traded in the UN Comtrade Database and individual long-tailed macaques reported in the CITES Trade Database, suggesting that we can use the UN Comtrade database to investigate values and magnitude of the international legal trade in wildlife, and that legal trade in live primates is primarily constituted of long-tailed macaques alone.

Southeast Asia is considered a global hotspot of emerging zoonotic diseases. There, wildlife is commonly traded under poor sanitary conditions in open markets; these markets have been considered ‘the perfect storm’ for zoonotic disease transmission. We assessed the potential of wildlife trade in spreading viral diseases by quantifying the number of wild animals of four mammalian orders (Rodentia, Chiroptera, Carnivora and Primates) on sale in 14 Indonesian wildlife markets and identifying zoonotic viruses potentially hosted by these animals. We constructed a network analysis to visualize the animals that are traded alongside each other that may carry similar viruses. We recorded 6725 wild animals of at least 15 species on sale. Cities and markets with larger human population and number of stalls, respectively, offered more individuals for sale. Eight out of 15 animal taxa recorded are hosts of 17 zoonotic virus species, nine of which can infect more than one species as a host. The network analysis showed that long-tailed macaque has the greatest potential for spreading viral diseases, since it is simultaneously the most traded species, sold in 13/14 markets, and a potential host for nine viruses. It is traded alongside pig-tailed macaques in three markets, with which it shares six viruses in common (Cowpox, Dengue, Hepatitis E, Herpes B, Simian foamy, and Simian retrovirus type D). Short-nosed fruit bats and large flying foxes are potential hosts of Nipah virus and are also sold in large quantities in 10/14 markets. This study highlights the need for better surveillance and sanitary conditions to avoid the negative health impacts of unregulated wildlife markets.

The evolution of venom and the selection pressures that act on toxins have been increasingly researched within toxinology in the last two decades, in part due to the exceptionally high rates of diversifying selection observed in animal toxins. In 2015, Sungar and Moran proposed the ‘two-speed’ model of toxin evolution linking evolutionary age of a group to the rates of selection acting on toxins but due to a lack of data, mammals were not included as less than 30 species of venomous mammal have been recorded, represented by elusive species which produce small amounts of venom. Due to advances in genomics and transcriptomics, the availability of toxin sequences from venomous mammals has been increasing. Using branch- and site-specific selection models, we present the rates of both episodic and pervasive selection acting upon venomous mammal toxins as a group for the first time. We identified seven toxin groups present within venomous mammals, representing Chiroptera, Eulipotyphla and Monotremata: KLK1, Plasminogen Activator, Desmallipins, PACAP, CRiSP, Kunitz Domain One and Kunitz Domain Two. All but one group (KLK1) was identified by our results to be evolving under both episodic and pervasive diversifying selection with four toxin groups having sites that were implicated in the fitness of the animal by TreeSAAP (Selection on Amino Acid Properties). Our results suggest that venomous mammal ecology, behaviour or genomic evolution are the main drivers of selection, although evolutionary age may still be a factor. Our conclusion from these results indicates that mammalian toxins are following the two-speed model of selection, evolving predominately under diversifying selection, fitting in with other younger venomous taxa like snakes and cone snails—with high amounts of accumulating mutations, leading to more novel adaptions in their toxins.

Edge effects result from interactions between adjacent habitats, which can modify abiotic and biotic conditions and produce various negative effects on biodiversity. Given the high degree of forest fragmentation in Madagascar, understanding lemur responses to edges is a conservation priority. We aim to determine the depth of edge influence in a continuous low-land rainforest of south-eastern Madagascar and identify the response of six lemur species. We surveyed lemur abundance along nine 1 km transects from May 2015 to July 2016 totaling 112.2 km of survey effort during the day and 88.5 km at night. We characterized the habitat structure via 33 plots centered along the line transects. We used Generalized Additive Models and Generalized Linear Models to test the effect of distance from the forest edge on vegetation parameters and animal encounter rates. Edge effect on the vegetation structure can be detected up to around 100 m in terms of tree diversity and density. We found a negative edge response for Madame Fleurette’s sportive lemurs (Lepilemur fleuretae) and collared brown lemurs (Eulemur collaris), and a positive edge response for Anosy mouse lemurs (Microcebus tanosi), Southern bamboo lemurs (Hapalemur meridionalis) and Southern woolly lemurs (Avahi meridionalis). Since around half of the forested areas in Madagascar are within 100 m of forest edge, taking into account edge effect is vital when producing estimates of population sizes and informing conservation management.

Southeast Asian box turtles Cuora amboinensis are distributed in mainland Southeast Asia and throughout most of insular Southeast Asia and are often found in habitats shared with humans. In the 2000s evidence emerged of an enormous illegal export of Southeast Asian box turtles from Indonesia estimated at a hundred times larger than the legal exports. Using publicly available data we show that one or two exporters in Sampit in the province of Central Kalimantan, one of nine provinces where harvest of Southeast Asian box turtles is authorised, continue to trade above permitted levels. Harvest quotas for Central Kalimantan are set at 1000 turtles a year, and this is divided between five approved traders, two of whom are based in Sampit. A single visit to one of these two traders in April 2019 documented the presence of 549 Southeast Asian box turtles. Based on documented data from middlemen we estimate that the number of Southeast Asian box turtles that are harvested in Central Kalimantan to supply the traders in Sampit amounts to 19,000–45,000 individuals a year. If the Sampit traders stay within their quotas potential profits are less than USD 400 year−1, compared to up to USD 40,000 year−1 when trading the higher numbers. It is not known how many box turtles are traded by the other three exporters in the province. With the annual harvest quota for all of Indonesia set at less than 15,000 the massive illegal trade as documented in the 1990s and 2000s continues unabated. Assessments of the harvest and trade in Southeast Asian box turtles must consider both the sustainability and legality of this trade.

The illegal wildlife trade is increasingly recognised as a major threat to biodiversity conservation, and one way of curbing it is to properly enforce existing legislation and where appropriate to prosecute to the full extent of the law. One of the taxa that suffers greatly from unregulated trade are the tortoises and freshwater turtles, with collection for trade currently being the most significant threat for most Asian species. Illegal trade in pig-nosed turtles from Indonesia to supply international demand for pets, and to a lesser degree for meat and use in traditional medicines, continues on a large scale. We examined 26 reported seizures over the period 2013 to 2020, of which 20 took place in Indonesia and the remaining five in other parts of Asia with the turtles being trafficked from Indonesia. In total, this amounted to the seizure of 52,374 pig-nosed turtles. Pig-nosed turtles are totally protected in Indonesia, and nine of the 26 cases we examined were successfully prosecuted though never to the full extent of the law. Trade in and trafficking of pig-nosed turtles is in violation of Indonesia’s Fisheries and Customs Acts, yet these legal instruments were not used for prosecutions. In addition to the illegal trade, the Indonesia CITES Management Authority reported the export of 5415 pig-nosed turtles, 95% of which were declared as wild-caught, in direct contravention of Indonesia’s own protected species legislation. We strongly recommend a strategy be developed and implemented to more effectively tackle this trade from point of collection through to point of sale, with improved use of all relevant pieces of national legislation to serve as a strong deterrent, and ultimately to protect this species from over-exploitation.

The Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) is the most important global initiative to monitor and regulate the international trade of plants and animals, but there is a lack of (retrospective) assessments of the effectiveness of its actions. We here focus on the international trade in Chinese hwamei Garrulax canorus, a songbird native to south-eastern China and northern Lao PDR and Vietnam. Prior to the year 2000, the species was heavily traded as an ornamental cage bird, both in countries within and outside its natural range. In an effort to prevent international overexploitation, at the request of China, the Chinese hwamei was listed in Appendix II of CITES, regulating all international trade. Here we compare data from three non-range countries (Thailand, Singapore and Indonesia) for which we have bird market survey data prior to and following the CITES listing. In addition, we assess whether or not CITES import and export figures agree with observations in the bird markets, and explore the contemporary (online) trade and trafficking in Chinese hwamei. We find a clear effect of the CITES listing and Chinese export restrictions in Indonesia (before ~50 birds/survey vs ~15 birds/survey after), but not in Thailand (~10 vs ~13) and Singapore (~4 vs ~16). Singapore is the only country that reports the import of Chinese hwamei, 1650 birds in 2002–2004 only, possibly accounting for a proportion of the birds observed in trade. In contrast, neither Thailand nor Indonesia report the import of a single Chinese hwamei since its CITES listing, despite some 2000 birds having been recorded openly for sale, suggesting large-scale illegal international trade in the species. Five seizures in Singapore and Indonesia were mostly because of concern about avian influenza. Price data, corrected for inflation, suggests that prices for Chinese hwamei have increased substantially, albeit following a delay, from ~US$50 prior to the listing to ~ US$200 after. We conclude that the CITES Appendix II listing of Chinese hwamei has had some positive effect on reducing the volumes of these birds in international trade, mainly through implementation of domestic legislation in China, but that substantial illegal or undocumented trade persists in Southeast Asia, involving organised criminal networks. We recommend actions from range countries, such as China, to cease the illegal export of Chinese hwamei, from importing countries such as Indonesia, to ensure no illegal shipments of birds enter their country, and for consumer countries where the species cannot legally be traded to curb the illegal domestic trade as the demand for songbirds in these markets drives the trafficking.

It is challenging to disentangle the legal and illegal aspects of wild-caught animals that are traded in wildlife markets or online, and this may diminish the value of conducting wildlife trade surveys. We present empirical studies on the trade in birds (ducks, owls, songbirds, non-passerines) in Indonesia (2005 to 2021). Based on visits to wildlife markets, wholesale traders, and monitoring of an Instagram account, we examine if five specific pieces of legislation (domestic and international) are adhered to: (1) protected species, (2) harvest quota, (3) welfare, (4) provincial transport restrictions, and (5) illegal import of CITES-listed species. Our five distinctly different case studies showed that in each case, certain rules and regulations were adhered to, whilst others were violated to varying degrees. When trade involved non-protected species, there was frequently a lack of harvest quotas or trade occurred above these allocated quotas. Basic welfare provisions were regularly and habitually violated. Visiting wildlife markets and recording first-hand what is openly offered for sale is a highly reliable, verifiable, and valuable method of data collection that can give insight in numerous aspects of the animal trade. Our research provides support for recognising the urgency for the government to take appropriate action to curb all the illegal aspects of the bird trade in Indonesia.

Pollination services, from both wild and managed populations of insect pollinators, have degraded as a result of agricultural intensification. Whilst 75% of economically important crops depend on insect pollinators for cultivation, 40% of insect pollinator species are threatened with extinction. Pollination services must be preserved if there is to be enough food for a global population whose demand is expected to double, if not triple, by 2050. Pollinator diversity and pollinator efficiency have been found to increase as a result of wildlife-friendly farming practices (i.e., natural chemicals and fertilizers and agroforestry). We evaluated the presence of insect pollinators in 42 coffee home gardens in West Java, Indonesia. Via generalized linear mixed models, we found that number of visitor species (β = 0.418 ± SE 0.194, p = 0.031) and visitation time (β = 0.845 ± SE 0.308, p = 0.006) decreased as farms were more intensely managed, (i.e., used chemical pesticides), compared to fields using organic practices. As knowledge of pollination services is widespread amongst smallholder farmers in Indonesia and beyond due to the long-held tradition of beekeeping, these results will add to their existing knowledge and empower farmers to enhance resources for pollinator species through agroforestry and natural pest management. Although we found significant differences in pollination services provided in intensely managed and wildlife-friendly farms, chemical use can affect farms far beyond a particular area of production. Therefore, pollinator conservation must be applied at a landscape level and involve all stakeholders, including farmers, when making effective policies.

Deforestation in the tropics is mainly driven by the need to expand agriculture and forestry land. Tropical cropland has also undergone a process of intensification, particularly evident in regions that are the main exporters of deforestation-driven commodities. Around 25 million people in the world depend on coffee production, which has a profound contribution to global biodiversity loss through agricultural extensification and intensification. Nevertheless, coffee agroforestry systems have been postulated to serve as an alternative refuge for biodiversity across different regions. We aim to compare bird abundance, diversity, and richness in commercial polyculture coffee systems (i.e., the highest degree of habitat complexity that can be achieved in coffee fields after deforestation) with other coffee agroforestry systems and human modified habitats in Java, Indonesia. We collected data in 21 sites (1228 points) on Java from February to August 2021 using the point sampling method. Via generalised additive models, we tested whether the abundance, diversity, and richness of birds were different between different human modified habitats including other potential predictors such as elevation, distance to protected areas, shade tree richness, and plant diversity. Using the non-metric multidimensional scaling, we tested whether there was a difference in terms of the composition of foraging guilds between habitats. Commercial polyculture coffee fields can sustain levels of bird abundance, diversity, and richness comparable to agroforestry systems under natural forest, and higher than sun coffee and shaded monoculture coffee, and of other human modified habitats such as crop/fruit fields and tree farms. Coffee agroforestry systems have a higher proportion of nectarivores, insectivores, and frugivores than other systems that can sustain high diversity and richness of birds such as paddy fields that mainly have granivores and carnivores. Complex polycultures can represent an avenue for the future of sustainable agriculture in conditions where deforestation rates are high and in crops such as coffee, which maintain high yield in the presence of diverse shade.

Social media has become an increasingly popular platform to trade legal and illegal wildlife. Here, we evaluate the online trade of otters, a group of globally threatened taxa in Thailand, a country of high global social media use. During the 14-month period, we monitored five Facebook groups to establish a primary understanding of the scope and scale of the trade. We recorded 160 sales posts (337 individual otters) of two species, the Asian small-clawed otter (Aonyx cinereus) (81%) and the smooth-coated otter (Lutrogale perspicillata) (19%). Newborn otter pups accounted for 53% of the offers, whereas young otters accounted for 35%. Prices averaged US$78, where the smooth-coated otter was offered at a significantly higher price than the Asian small-clawed otter. Juvenile otters were also significantly more expensive than newborns. Trade appears to be domestic; however, the potential for international trade cannot be overlooked. Although otters are protected domestically, we find that the trade is easily accessible and prevalent. The results reflect current inadequacies in enforcement and legislation in keeping pace with the rapidly shifting nature of the Internet in Thailand and throughout the global Internet community. A consistent collaborative effort from consumers, enforcement agencies, and operators is required to address this illicit trade. Keywords: Exotic pets, Facebook, Illegal wildlife trade, Otters, Social media

The wildlife trade has facilitated the introduction of invasive non-native species, which may compete with native species for resources and alter ecosystems. Some of these species have great potential to become invasive if released or escaped from captivity. Here we studied the pet trade in a group of open countryside birds, the mynas (Acridotheres spp.) in Indonesia, and identified the areas that are at high risk of facing the establishment of these species. Mynas are among the most invasive birds in Southeast Asia. Once established in a new area, they are almost impossible to eradicate and can have strong negative impacts on the ecosystem. Preventing their introduction is therefore essential. Yet, invasive non-native mynas continue to be traded openly. We present data on the trade in seven species of mynas on Java, Bali and Lombok, with three species being native to parts of one or two of these islands, but not to the remainder, and four that are non-native to the region. From 2016 to 2021 we conducted 255 surveys of 30 animal markets. We recorded over 6000 mynas that were offered for sale outside their native range. Areas most at risk because of their high prevalence in specific animal markets, are Greater Jakarta, eastern Java, Bali and Lombok. The number of invasive non-native mynas recorded was positively related to the size of the animal market. Indonesia is signatory to several international agreements (CBD, ASEAN) that have policies and guidelines to prevent the introduction of invasive non-native species, but compliancy is weak. Annually hundreds and possibly thousands of invasive non-native mynas are released by Indonesian conservation authorities in regions that are outside their native range. Effective management of, and regulation of trade in, potential invasive non-native birds in Indonesia falls short and inadvertently greatly aids both their introduction and establishment.

Hundreds of species of wild-caught birds are offered for sale in the bird markets of Java and Bali, Indonesia, to meet the demand for the largely-domestic pet and songbird trade. In the past, owls were offered only in very small numbers in these bird markets but since the release of the Harry Potter series in Indonesia in the early 2000s their popularity as pets has increased. Whereas in the past owls were collective known as Burung Hantu (“Ghost birds”), in the bird markets they are now commonly referred to as Burung Harry Potter (“Harry Potter birds”). We made a retrospective quantitative assessment of the abundance of owls in the bird markets (1979–2010) and conducted 109 surveys in 20 bird markets in 2012–2016 to quantify owls in trade. In the 1980s, 1990s and early 2000s owls were rarely recorded in Indonesia's bird markets, typically one or two and up to five per survey, and frequently no owls were recorded at all. The trade was largely confined to small scops owls. In the late 2000s more species were offered for sale, including barn and bay owls, and larger owl species such as wood-owls, eagle-owls and fish-owls; typically 10 + owls were observed per survey. In recent years, the number of owl species increased even more, and on average we recorded 17 owls per survey, yielding a total of 1810 owls, and in >90% of the surveys owls were present. In the larger bird markets in Jakarta and Bandung typically 30 to 60 owls are on offer of up to 8 species at a time. The number of owls as a proportion of all birds in the markets increased from 0.43% post 2008, suggesting a delayed Harry Potter effect. Over this period, common species have become cheaper and less common ones have become more expensive. The owls are largely, if not exclusively, wild-caught and are sold into the domestic pet market. The release of Harry Potter films and novels in Indonesia coincided with the rise of the Internet and social media and, with some delay, the emergence of pet owl interest groups on Java and Bali, thus preventing us to demonstrate a causal Harry Potter effect on the owl trade. The overall popularity of owls as pets in Indonesia has risen to such an extent that it may imperil the conservation of some of the less abundant species. Inclusion of owls on Indonesia's protected species list, alongside all diurnal raptors, may be a first step to mitigate the negative effects of this emerging trend.

The northern Myanmar region has been identified as a potential transit and source place for the illegal trade of pangolins and their scales. In this study, we surveyed the trade links between Kachin State (northern Myanmar) and China and Kachin and India based on interviews, market surveys and online seizure data. From our results we cannot extrapolate that there is a link between Myanmar and India. Based on the results from interviews (17 of 38), we found that around 140–168 pangolins/year are smuggled into China via three different routes from Kachin to China. Scales are the most traded parts of pangolins in this part of Myanmar. Based on the online sources, 30 seizures of pangolin and their products were made on the Kachin–China route during 2010–2016, with all seizures made on the Chinese side of the border. We thus, recommend an increase in law enforcement on the Myanmar side, with focused effort at identifying trade hubs and deterring wholesalers. We further suggest investigating possible trade links between Kachin and other source areas. We recommend, a reclassification of the pangolins’ protection status in China from a Class II to a Class I Key Protected Species, and the prohibition of the use of pangolins’ scales for Traditional Chinese Medicine.

The rise of palm oil as the world's most consumed vegetable oil has coincided with exponential growth in palm oil research activity. Bibliometric analysis of research outputs reveals a distinct imbalance in the type of research being undertaken, notably a disproportionate focus on biofuel and engineering topics. Recognizing the expansion of oil palm agriculture across the tropics and the increasing awareness of environmental, social, and economic impacts, we seek to reorientate the existing research agenda toward one that addresses the most fundamental and urgent questions defined by the palm oil stakeholder community. Following consultation with 659 stakeholders from 38 countries, including palm oil growers, government agencies, non-governmental organizations, and researchers, the highest priority research questions were identified within 13 themes. The resulting 279 questions, including 26 ranked as top priority, reveal a diversity of environmental and social research challenges facing the industry, ranging from the ecological and ecosystem impacts of production, to the livelihoods of plantation workers and smallholder communities. Analysis of the knowledge type produced from these questions underscores a clear need for fundamental science programmes, and studies that involve the consultation of non-academic stakeholders to develop “transformative” solutions to the oil palm sector. Stakeholders were most aligned in their choice of priority questions across the themes of policy and certification related themes, and differed the most in environmental feedback, technology and smallholder related themes. Our recommendations include improved regional academic leadership and coordination, greater engagement with private and public stakeholders in Africa, and Central and South America, and enhanced collaborative efforts with researchers in the major consuming countries of India and China.

Apes continue to be trafficked to meet the demand for pets or zoos. Indonesia, the most diverse country in terms of ape species, has been implicated in the global trade in gibbons, orangutans and, to a lesser degree, chimpanzees. Recently trade has shifted to online platforms, a trend that may have been amplified by the Covid-19 pandemic and partial lockdowns. We assessed the availability of legally protected apes for sale on Facebook and Instagram over two 16-months periods (2017–2018 and 2020–2021). Despite Facebook and Instagram explicitly banning the sale of endangered animals, and Facebook not allowing the sale of live animals, we found 106 gibbons, 17 orangutans and four chimpanzees for sale on five Facebook pages and 19 Instagram accounts. All orangutans and chimpanzees and 70% of the gibbons were infants or juveniles. We did not record any obvious responses of vendors to the Covid-19 pandemic. Facebook and Instagram accounts were linked (similar names, cross-referencing each other and announcing new accounts on existing ones), names were altered (e.g., “petshop” to “pethsop”) and new vendors emerged for short periods. Facebook and Instagram’s policy of not allowing the sale of live and/or endangered wildlife on their platforms is not effectively implemented in Indonesia.

Indonesia is at the epicenter of the Asian Songbird Crisis, i.e., the recognition that the cage bird trade has a devastating impact on numerous imperiled bird species in Asia. The Javan pied starling Gracupica jalla, only in the last five years recognized as distinct from the pied starlings of mainland Southeast Asia, has been declared extinct the wild in 2021. Up until the 1980s, it used to be one of the most common open countryside birds on the islands of Java and Bali, Indonesia. From the early 2000s onwards, the species is commercially bred to meet the demand from the domestic cagebird trade. We conducted 280 market surveys in 25 bird markets in Java and Bali between April 2014 and March 2020, with 15 markets being surveyed at least six times. We recorded 24,358 Javan pied starlings, making it one of the most commonly observed birds in the markets. We established that, conservatively, around 40% of the birds in the market were sold within one week and used this to estimate that at a minimum ~80,000 Javan pied starlings are sold in the bird markets on Java and Bali. The latter represents a monetary value of USD5.2 million. We showed that prices were low in the 1980s, when all birds were sourced from the wild. It became more varied and differentiated in the 2000s when a combination of now expensive wild-caught and cheaper captive-bred birds were offered for sale, and prices stabilized in the 2010s when most, if not all birds were commercially captive-bred. Javan pied starlings are not protected under Indonesian law, and there are no linked-up conservation efforts in place to re-establish a wild population on the islands, although small-scale releases do take place.

There is an urgent need for a global transition to sustainable and wildlife-friendly farming systems that provide social and economic equity and protect ecosystem services on which agriculture depends. Java is home to 60% of Indonesia’s population and harbors many endemic species; thus, managing agriculture alongside human well-being and biodiversity is vital. Within a community of ~400 coffee farmers in the province of West Java, we assessed the steps to develop a wildlife-friendly program until reaching certification between February 2019 and October 2020. We adopted an adaptive management approach that included developing common objectives through a process of stakeholder consultation and co-learning. We firstly investigated via interviews the expectations and the issues encountered by 25 farmers who converted to organic production in 2016. Their main expectations were an increase in income and an increase in coffee quality, while they had issues mainly in finding high quality fertilizers, reducing pests, and increasing productivity. We used this information to establish a problem-solving plan for the transition to community-wide wildlife-friendly practices. As part of the adaptive evaluation, we assessed the quality of coffee plantations before and after the implementation of coproduced actions. The quality of coffee significantly improved after our interventions to reduce the coffee berry borer, especially in the fields that started as inorganic and converted to organic. We uncovered additional issues to meet the standards for certification, including banning hunting and trapping activities and increasing coffee quality for international export. We describe the coproduced actions (agroforestry, conservation education, local law, organic alternatives) and phases of the program and discuss the potential barriers. We provide novel evidence of adaptive management framework successfully used to implement management actions and reach shared goals.

Slow lorises are enigmatic animal that represent the only venomous primate lineage. Their defensive secretions have received little attention. In this study we determined the full length sequence of the protein secreted by their unique brachial glands. The full length sequences displayed homology to the main allergenic protein present in cat dander. We thus compared the molecular features of the slow loris brachial gland protein and the cat dander allergen protein, showing remarkable similarities between them. Thus we postulate that allergenic proteins play a role in the slow loris defensive arsenal. These results shed light on these neglected, novel animals.

The illegal cage bird trade is increasingly recognised as a major impediment to the survival of a large number of songbirds. Indeed some bird species are now more common in captive private hands than they are in the wild. This includes the black-winged mynas (Acridotheres melanopterus, A. tricolor and A. tertius), three species of Critically Endangered songbirds endemic to Indonesia. Only 20 years ago these species were not considered globally threatened but high levels of trapping from the wild for the largely domestic cage bird trade has brought all three species to the brink of extinction. It is estimated that less than 500 black-winged mynas remain in the wild. Here we investigate the trade in black-winged mynas, online and in bird markets, and make an assessment of the role captive breeding played in the conservation and management of the species over the period 2009–2018. We found prices peaked in 2014 at ∼ US$140 (∼75% of the monthly minimum wage) per bird and have subsequently gone down to US$85 (now ∼33% of the minimum wage), possibly indicating higher supply and lower demand. In 2015–2018 we surveyed seven bird markets in western Java and in 127/145 visits observed 1253 black-winged mynas for sale. Turnover was high, with ∼50% of birds sold after one week upon arrival in the market. We estimate that between 1300 and 2300 mynas (retail value ∼ US$170,000) are sold annually in these seven bird markets. Few birds had closed leg-rings, but were in all likelihood a combination of captive-bred, first-generation captive-born and wild-caught individuals; some appeared to be crossbreeds between the three recognised species. With additional bird markets in Java and Bali and a thriving online trade, we estimate that the number of black-winged mynas in private ownership in Indonesia is in the order of 40,000 birds. Without proper registration and regulation in the trade of captive-bred mynas, even a small amount of wild-caught birds entering this now substantial trade will act as a serious impediment to the conservation of black-winged mynas. With the species already being ecologically extinct, we anticipate that it soon will join the ranks of species like Père David's deer Elaphurus davidianus and scimitar-horned oryx Oryx dammah that are extinct in the wild but that have captive populations in the tens of thousands. In order to prevent the imminent extinction of black-winged mynas in the wild, we recommend (1) that the Indonesian authorities invest in more effective law enforcement and prosecution of lawbreakers; (2) establishing a multi-stakeholder three species black-winged myna management plan, in which commercial captive breeders participate; and (3) better coordination of reintroduction programmes. Keywords: CITES, Conservation, Hybridisation, Indonesia, Introgression, E-commerce, Wildlife trade

Primates occur in 90 countries, but four—Brazil, Madagascar, Indonesia, and the Democratic Republic of the Congo (DRC)—harbor 65% of the world’s primate species (439) and 60% of these primates are Threatened, Endangered, or Critically Endangered (IUCN Red List of Threatened Species 2017-3). Considering their importance for global primate conservation, we examine the anthropogenic pressures each country is facing that place their primate populations at risk. Habitat loss and fragmentation are main threats to primates in Brazil, Madagascar, and Indonesia. However, in DRC hunting for the commercial bushmeat trade is the primary threat. Encroachment on primate habitats driven by local and global market demands for food and non-food commodities hunting, illegal trade, the proliferation of invasive species, and human and domestic-animal borne infectious diseases cause habitat loss, population declines, and extirpation. Modeling agricultural expansion in the 21st century for the four countries under a worst-case-scenario, showed a primate range contraction of 78% for Brazil, 72% for Indonesia, 62% for Madagascar, and 32% for DRC. These pressures unfold in the context of expanding human populations with low levels of development. Weak governance across these four countries may limit effective primate conservation planning. We examine landscape and local approaches to effective primate conservation policies and assess the distribution of protected areas and primates in each country. Primates in Brazil and Madagascar have 38% of their range inside protected areas, 17% in Indonesia and 14% in DRC, suggesting that the great majority of primate populations remain vulnerable. We list the key challenges faced by the four countries to avert primate extinctions now and in the future. In the short term, effective law enforcement to stop illegal hunting and illegal forest destruction is absolutely key. Long-term success can only be achieved by focusing local and global public awareness, and actively engaging with international organizations, multinational businesses and consumer nations to reduce unsustainable demands on the environment. Finally, the four primate range countries need to ensure that integrated, sustainable land-use planning for economic development includes the maintenance of biodiversity and intact, functional natural ecosystems.

Hedgehogs are traded locally and often in relatively small num--bers throughout Eurasia and Africa. We here report on the trade in North African Hedgehog Atelerix algirus and to a smaller extent possibly the Desert Hedgehog Paraechinus aethiopicus in Morocco, and provide an overview of the global trade in hedgehogs for medicinal purposes. In 2013 and 2014 we surveyed 20 Moroccan cities for a total of 48 times. We recorded 114 hedgehogs (32 alive and 82 skins) for sale in 25 shops in 10 cities, with the largest numbers recorded in Casablanca and Marrakesh. All live hedgehogs were identified as North African Hedgehog but skins could additionally have been of the Desert Hedgehog. Shops often displayed only single hedgehog skins, but occasionally up to 48 skins, and live individuals were mostly traded singly or in pairs. Over 80% of the shops selling hedgehogs were herbalists, selling herbs, spices, oils and animal parts, and both skins and live hedgehogs were intended to supply the demand for traditional (‘folk’) medicine. At a global scale we found an additional 34 reports of trade in 12 or possibly 13 species of hedgehogs from 23 countries; five studies involving three species in China, South Africa and Benin, included data on the frequency and abundance of hedgehogs in trade, whereas the other studies were qualitative in nature. Market data have limited value in gauging the off-take of hedgehogs from the wild to supply the traditional medicine trade, but we nevertheless urge the continuation of monitoring the trade in hedgehogs in Morocco and indeed elsewhere to ensure it does not become a threat to their survival in the foreseeable future.

The function of colouration in animals includes concealment, communication and signaling, such as the use of aposematism as a warning signal. Aposematism is unusual in mammals, and exceptions help us to understand its ecology and evolution. The Javan slow loris is a highly territorial venomous mammal that has a distinctive facial mask and monochromatic vision. To help understand if they use aposematism to advertise their venom to conspecifics or predators with different visual systems, we studied a population in Java, Indonesia. Using ImageJ, we selected colours from the facial masks of 58 individuals, converted RBG colours into monochromatic, dichromatic and trichromatic modes, and created a contrast index. During 290 captures, we recorded venom secretion and aggressiveness. Using Non-metric Multidimensional Scaling and generalised additive models for location, scale and shape, we found that young slow lorises differ significantly from adults, being both more contrasting and more aggressive, with aggressive animals showing fewer wounds. We suggest aposematic facial masks serve multiple purposes in slow lorises based on age. Change in colouration through development may play a role in intraspecific competition, and advertise toxicity or aggressiveness to competitors and/or predators in juveniles. Aposematic signals combined with intraspecific competition may provide clues to new venomous taxa among mammals.

The mitochondrial cytochrome c-oxidase subunit I (COI) can serve as a fast and accurate marker for the identification of animal species, and has been applied in a number of studies on birds. We here sequenced the COI gene for 387 individuals of 147 species of birds from the Netherlands, with 83 species being represented by >2 sequences. The Netherlands occupies a small geographic area and 95% of all samples were collected within a 50 km radius from one another. The intraspecific divergences averaged 0.29% among this assemblage, but most values were lower; the interspecific divergences averaged 9.54%. In all, 95% of species were represented by a unique barcode, with 6 species of gulls and skua (Larus and Stercorarius)at least one shared barcode. This is best explained by these species representing recent radiations with ongoing hybridization. In contrast, one species, the Lesser Whitethroat Sylvia curruca showed deep divergences, averaging 5.76% and up to 8.68% between individuals. These possibly represent two distinct taxa, S. curruca and S. blythi, both clearly separated in a haplotype network analysis. Our study adds to a growing body of DNA barcodes that have become available for birds, and shows that a DNA barcoding approach enables to identify known Dutch bird species with a very high resolution. In addition some species were flagged up for further detailed taxonomic investigation, illustrating that even in ornithologically well-known areas such as the Netherlands, more is to be learned about the birds that are present.

BACKGROUND:Tropical marine molluscs are traded globally. Larger species with slow life histories are under threat from over-exploitation. We report on the trade in protected marine mollusc shells in and from Java and Bali, Indonesia. Since 1987 twelve species of marine molluscs are protected under Indonesian law to shield them from overexploitation. Despite this protection they are traded openly in large volumes. METHODOLOGY/PRINCIPAL FINDINGS:We collected data on species composition, origins, volumes and prices at two large open markets (2013), collected data from wholesale traders (2013), and compiled seizure data by the Indonesian authorities (2008-2013). All twelve protected species were observed in trade. Smaller species were traded for 32,000 shells valued at USD500,000), chambered nautilus (Nautilus pompilius) (>3,000 shells, USD60,000) and giant clams (Tridacna spp.) (>2,000 shells, USD45,000) were traded in largest volumes. Two-thirds of this trade was destined for international markets, including in the USA and Asia-Pacific region. CONCLUSIONS/SIGNIFICANCE:We demonstrated that the trade in protected marine mollusc shells in Indonesia is not controlled nor monitored, that it involves large volumes, and that networks of shell collectors, traders, middlemen and exporters span the globe. This impedes protection of these species on the ground and calls into question the effectiveness of protected species management in Indonesia; solutions are unlikely to be found only in Indonesia and must involve the cooperation of importing countries.