1. Polymeric nanoparticles loaded with vincristine and carbon dots for hepatocellular carcinoma therapy and imaging.
- Author
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Fawaz W, Hanano A, Murad H, Yousfan A, Alghoraibi I, and Hasian J
- Subjects
- Humans, Animals, Mice, Quantum Dots chemistry, Cell Survival drug effects, Hep G2 Cells, Cell Line, Tumor, Drug Carriers chemistry, Antineoplastic Agents, Phytogenic administration & dosage, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic chemistry, Polymers chemistry, Hepatocytes drug effects, Hepatocytes metabolism, Polyesters chemistry, Vincristine chemistry, Vincristine administration & dosage, Vincristine pharmacology, Vincristine therapeutic use, Carcinoma, Hepatocellular drug therapy, Carcinoma, Hepatocellular diagnostic imaging, Carcinoma, Hepatocellular pathology, Liver Neoplasms drug therapy, Liver Neoplasms diagnostic imaging, Carbon chemistry, Nanoparticles chemistry
- Abstract
Chemotherapy for hepatoblastoma is limited by organ toxicity and poor outcomes, prompting the search for new, more effective treatments with minimal side effects. Vincristine sulfate, a potent chemotherapeutic, faces challenges due to P-glycoprotein-mediated resistance and its systemic toxicity. Nanoparticles offer a promising solution by improving pharmacokinetics, targeting tumor cells, thus reducing side effects. Moreover, the use of fluorescent nanomaterials is emerging in biomedical applications such as bioimaging, detection and therapies. This study describes a promising delivery system utilizing carbon dots encapsulated with vincristine in biodegradable polycaprolactone nanoparticles via a double emulsion technique. The fine characterization of these nanoparticles showed that they are spherical, uniformly sized with around 200 nm and exhibit excellent colloidal stability. Moreover, the release profile showed prolonged release for both vincristine and carbon dots. In vitro cell viability studies revealed enhanced cancer cell inhibition for the encapsulated drug compared to the vincristine solution. The uptake study indicated clear fluorescence for carbon dots solution and vincristine and carbon dots loaded nanoparticles upon excitation. Additionally, studies on primary mouse hepatocytes demonstrated higher fluorescence intensity in treatment groups. These results suggest that vincristine and carbon dots loaded nanoparticles are effective, target-specific carriers for liver cancer treatment. Furthermore, the carbon dots were not cytotoxic, highlighting their potential in bioimaging and cancer cell studies., (© 2024. The Author(s).)
- Published
- 2024
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