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We investigated the design and characterization of a Lab-On-a-Chip (LoC) cell detection system primarily designed to support immunotherapy in cancer treatment. Immunotherapy uses Chimeric Antigen Receptors (CARs) and T Cell Receptors (TCRs) to fight cancer, engineering the response of the immune system. In recent years, it has emerged as a promising strategy for personalized cancer treatment. However, it requires bioreactor-based cell culture expansion and manual quality control (QC) of the modified cells, which is time-consuming, labour-intensive, and prone to errors. The miniaturized LoC device for automated QC demonstrated here is simple, has a low cost, and is reliable. Its final target is to become one of the building blocks of an LoC for immunotherapy, which would take the place of present labs and manual procedures to the benefit of throughput and affordability. The core of the system is a commercial, on-chip-integrated capacitive sensor managed by a microcontroller capable of sensing cells as accurately measured charge variations. The hardware is based on standardized components, which makes it suitable for mass manufacturing. Moreover, unlike in other cell detection solutions, no external AC source is required. The device has been characterized with a cell line model selectively labelled with gold nanoparticles to simulate its future use in bioreactors in which labelling can apply to successfully engineered CAR-T-cells. Experiments were run both in the air—free drop with no microfluidics—and in the channel, where the fluid volume was considerably lower than in the drop. The device showed good sensitivity even with a low number of cells—around 120, compared with the 107 to 108 needed per kilogram of body weight—which is desirable for a good outcome of the expansion process. Since cell detection is needed in several contexts other than immunotherapy, the usefulness of this LoC goes potentially beyond the scope considered here. [ABSTRACT FROM AUTHOR]

The Internet of Things (IoT) has radically changed the industrial world, enabling the integration of numerous systems and devices into the industrial ecosystem. There are many areas of the manufacturing industry in which IoT has contributed, including plants' remote monitoring and control, energy efficiency, more efficient resources management, and cost reduction, paving the way for smart manufacturing in the framework of Industry 4.0. This review article provides an up-to-date overview of IoT systems and machine learning (ML) algorithms applied to smart manufacturing (SM), analyzing four main application fields: security, predictive maintenance, process control, and additive manufacturing. In addition, the paper presents a descriptive and comparative overview of ML algorithms mainly used in smart manufacturing. Furthermore, for each discussed topic, a deep comparative analysis of the recent IoT solutions reported in the scientific literature is introduced, dwelling on the architectural aspects, sensing solutions, implemented data analysis strategies, communication tools, performance, and other characteristic parameters. This comparison highlights the strengths and weaknesses of each discussed solution. Finally, the presented work outlines the features and functionalities of future IoT-based systems for smart industry applications. [ABSTRACT FROM AUTHOR]

Within an industrial plant, the handling of randomly arranged objects is becoming increasingly popular. The technology industry has introduced ever more powerful devices to the market, but they are often unable to meet the demands of the industry in terms of processing times. Using a multi-component feeder, which facilitates the automatic picking of objects arranged in bulk, is the ideal element to speed up the identification of objects by the vision system. The innovative designed feeder eliminates the dead time of the vision system since the feeder has two working surfaces, thus making the viewing time hidden in relation to the total handling cycle time. In addition, the step feeder integrated into the feeder structure allows for control over the number of objects that fall onto the work surface, optimizing the material flow. The feeder was designed to palletize aluminum hinge fins but can also handle other products with different shapes and sizes. A two-dimensional (2D) vision system is integrated into the robotic cell to identify the components to be palletized, obtaining a reduced cycle time. The innovative feeder is fully adaptable to industrial applications and allows for easy integration into the robotic cell in which it is installed; by testing its operation with different aluminum fins, male and female, significant results were obtained in terms of cycle times ranging from 1.44 s to 1.68 s per piece, with an average productivity level (PL) of 1175 pcs every 30 min. [ABSTRACT FROM AUTHOR]

This research explores the application of wireless sensor networks for the non-invasive monitoring of sleep quality and vital signs in elderly individuals, addressing significant challenges faced by the aging population. The study implemented and evaluated WSNs in home environments, focusing on variables such as breathing frequency, deep sleep, snoring, heart rate, heart rate variability (HRV), oxygen saturation, Rapid Eye Movement (REM sleep), and temperature. The results demonstrated substantial improvements in key metrics: 68% in breathing frequency, 68% in deep sleep, 70% in snoring reduction, 91% in HRV, and 85% in REM sleep. Additionally, temperature control was identified as a critical factor, with higher temperatures negatively impacting sleep quality. By integrating AI with WSN data, this study provided personalized health recommendations, enhancing sleep quality and overall health. This approach also offered significant support to caregivers, reducing their burden. This research highlights the cost-effectiveness and scalability of WSN technology, suggesting its feasibility for widespread adoption. The findings represent a significant advancement in geriatric health monitoring, paving the way for more comprehensive and integrated care solutions. [ABSTRACT FROM AUTHOR]

Aim of the present manuscript is to provide an overview of all possible methods and light source typologies used by the different research groups for obtaining the energetic nano-materials’ photo-ignition, showing the latest progress related to such phenomenon employing, also, alternative radiation sources to the common Xe lamp. In fact, the employment of a different source typology can open new usage prospects respect to those enabled by the Xe lamp, mainly due to its technological limitations. Therefore, several studies are faced to test light sources, such as lasers and LEDs, for igniting the nano-energetic materials (as CNTs mixed with metallic catalyzers, Al / CuO nano-particles, etc); these nano-materials are usefully employed for starting, in volumetric and controlled way, the combustion of air-fuel mixtures inside internal combustion engines, leading to significant benefits to the combustion process also in terms of efficiency, reliability, and emissions of pollutants. Several research works are presented in literature concerning the ignition of liquid / gaseous fuels, without nano-particles, employing laser sources (i.e laser-based plugs in place of the common spark plugs); therefore, an innovative solution is proposed that employs multi-point laser-plugs for inducing the ignition of nano-materials dispersed into the air-fuel mixture inside the cylinder, so further improving the combustion of the fuel in an internal combustion engine.

Accurate prognosis and diagnosis are crucial for selecting and planning lung cancer treatments. As a result of the rapid development of medical imaging technology, the use of computed tomography (CT) scans in pathology is becoming standard practice. An intricate interplay of requirements and obstacles characterizes computer-assisted diagnosis, which relies on the precise and effective analysis of pathology images. In recent years, pathology image analysis tasks such as tumor region identification, prognosis prediction, tumor microenvironment characterization, and metastasis detection have witnessed the considerable potential of artificial intelligence, especially deep learning techniques. In this context, an artificial intelligence (AI)-based methodology for lung cancer diagnosis is proposed in this research work. As a first processing step, filtering using the Butterworth smooth filter algorithm was applied to the input images from the LUNA 16 lung cancer dataset to remove noise without significantly degrading the image quality. Next, we performed the bi-level feature selection step using the Chaotic Crow Search Algorithm and Random Forest (CCSA-RF) approach to select features such as diameter, margin, spiculation, lobulation, subtlety, and malignancy. Next, the Feature Extraction step was performed using the Multi-space Image Reconstruction (MIR) method with Grey Level Co-occurrence Matrix (GLCM). Next, the Lung Tumor Severity Classification (LTSC) was implemented by using the Sparse Convolutional Neural Network (SCNN) approach with a Probabilistic Neural Network (PNN). The developed method can detect benign, normal, and malignant lung cancer images using the PNN algorithm, which reduces complexity and efficiently provides classification results. Performance parameters, namely accuracy, precision, F-score, sensitivity, and specificity, were determined to evaluate the effectiveness of the implemented hybrid method and compare it with other solutions already present in the literature. [ABSTRACT FROM AUTHOR]

The phonocardiogram (PCG) can be used as an affordable way to monitor heart conditions. This study proposes the training and testing of several classifiers based on SVMs (support vector machines), k-NN (k-Nearest Neighbor), and NNs (neural networks) to perform binary ("Normal"/"Pathologic") and multiclass ("Normal", "CAD" (coronary artery disease), "MVP" (mitral valve prolapse), and "Benign" (benign murmurs)) classification of PCG signals, without heart sound segmentation algorithms. Two datasets of 482 and 826 PCG signals from the Physionet/CinC 2016 dataset are used to train the binary and multiclass classifiers, respectively. Each PCG signal is pre-processed, with spike removal, denoising, filtering, and normalization; afterward, it is divided into 5 s frames with a 1 s shift. Subsequently, a feature set is extracted from each frame to train and test the binary and multiclass classifiers. Concerning the binary classification, the trained classifiers yielded accuracies ranging from 92.4 to 98.7% on the test set, with memory occupations from 92.7 kB to 11.1 MB. Regarding the multiclass classification, the trained classifiers achieved accuracies spanning from 95.3 to 98.6% on the test set, occupying a memory portion from 233 kB to 14.1 MB. The NNs trained and tested in this work offer the best trade-off between performance and memory occupation, whereas the trained k-NN models obtained the best performance at the cost of large memory occupation (up to 14.1 MB). The classifiers' performance slightly depends on the signal quality, since a denoising step is performed during pre-processing. To this end, the signal-to-noise ratio (SNR) was acquired before and after the denoising, indicating an improvement between 15 and 30 dB. The trained and tested models occupy relatively little memory, enabling their implementation in resource-limited systems. [ABSTRACT FROM AUTHOR]

Vibration analysis is commonly used to assess machinery conditions, earthquake detection, and structural monitoring. Commercially available DAQs (Data Acquisition Systems) feature high costs and limited versatility in terms of end-user hardware/firmware customization, making it difficult to adapt them to the input signal features and add supplementary functionalities. Hence, this research aims to develop a custom acquisition board for detecting vibration signals via IEPE (Integrated Electronic Piezoelectric) sensors, considering the limitations of commercially available systems, and building upon solutions found in the literature. The DAQ board was intended for remote vibration monitoring of infrastructure and machinery for industrial applications, allowing the implementation of predictive maintenance strategies. The proposed DAQ board has two independent and fully configurable channels, which can be set for acquiring signals from IEPE sensors or generic voltage sources. The DAQ board relies on the STM32F401 microcontroller to manage the acquisition from high-speed ADCs, process data, and store them in mass memory (SD card). During acquisition, the DAQ implements a batch acquisition strategy based on a buffer flash memory for temporarily storing ADCs data, which are iteratively poured into mass memory. Also, the board has Bluetooth connectivity to transmit acquired data and receive commands remotely. A prototype of the DAQ board was developed and tested with several waveforms, including vibration signals. The tests showed that the board can acquire vibration signals and compute the FFT onboard. The DAQ demonstrated a good balance between performance, accuracy, flexibility, and cost, making it suitable for several industrial applications and allowing for scalability and integration potential. [ABSTRACT FROM AUTHOR]

Detection of leakages in pipelines is a matter of continuous research because of the basic importance for a waterworks system is finding the point of the pipeline where a leak is located and − in some cases − a nature of the leak. There are specific difficulties in finding leaks by using spectral analysis techniques like FFT (Fast Fourier Transform), STFT (Short Term Fourier Transform), etc. These difficulties arise especially in complicated pipeline configurations, e.g. a zigzag one. This research focuses on the results of a new algorithm based on FFT and comparing them with a developed STFT technique. Even if other techniques are used, they are costly and difficult to be managed. Moreover, a constraint in the leak detection is the pipeline diameter because it influences accuracy of the adopted algorithm. FFT and STFT are not fully adequate for complex configurations dealt with in this paper, since they produce ill-posed problems with an increasing uncertainty. Therefore, an improved Tikhonov technique has been implemented to reinforce FFT and STFT for complex configurations of pipelines. Hence, the proposed algorithm overcomes the aforementioned difficulties due to applying a linear algebraic approach.