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1. Improved PCR-RFLP for the Detection of Diminazene Resistance in Trypanosoma congolense under Field Conditions Using Filter Papers for Sample Storage.

Author

Vitouley, HervéSèna, Mungube, Erick Ouma, Allegye-Cudjoe, Emmanuel, Diall, Oumar, Bocoum, Zakaria, Diarra, Boucader, Randolph, Thomas F., Bauer, Burkhard, Clausen, Peter-Henning, Geysen, Dirk, Sidibe, Issa, Bengaly, Zakaria, Van den Bossche, Peter, and Delespaux, Vincent

Subjects
*DIAGNOSTIC use of polymerase chain reaction, *RESTRICTION fragment length polymorphisms, *DIMINAZENE aceturate, *TRYPANOSOMA, *TRYPANOSOMIASIS in animals, *GENE amplification, *DRUG resistance, *FILTERS & filtration
Abstract

The article focuses on the improvement of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) for diminazene resistance detection in trypanosoma congolense. It offers information on the animal African trypanosomiasis and discusses the different techniques for trypanocidal drug resistance diagnosis. It outlines the advantages and disadvantages of the use of Dpll-PCR-RFLP through the use of filter papers . It indicates that the use of filter papers faciliate the large scale survey implementation for trypanocidal drug resistance in African countries affected by the trypanosomiasis using molecular diagnostic tools.

Published
2011
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