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2 results on '"Viveksheel Malhotra"'

1. Development and evaluation of novel bio-safe filter paper-based kits for sputum microscopy and transport to directly detect Mycobacterium tuberculosis and associated drug resistance.

Author

Divya Anthwal, Surabhi Lavania, Rakesh Kumar Gupta, Ajoy Verma, Vithal Prasad Myneedu, Prem Prakash Sharma, Hitesh Verma, Viveksheel Malhotra, Ashawant Gupta, Nalini Kant Gupta, Rohit Sarin, Sagarika Haldar, and Jaya Sivaswami Tyagi

Subjects
Medicine, Science
Abstract

India has the highest burden of Tuberculosis (TB) and multidrug-resistant TB (MDR-TB) worldwide. Innovative technology is the need of the hour to identify these cases that remain either undiagnosed or inadequately diagnosed due to the unavailability of appropriate tools at primary healthcare settings. We developed and evaluated 3 kits, namely 'TB Detect' (containing BioFM-Filter device), 'TB Concentration and Transport' (containing Trans-Filter device) and 'TB DNA Extraction' kits. These kits enable bio-safe equipment-free concentration of sputum on filters and improved fluorescence microscopy at primary healthcare centres, ambient temperature transport of dried inactivated sputum filters to central laboratories and molecular detection of drug resistance by PCR and DNA sequencing (Mol-DST). In a 2-site evaluation (n = 1190 sputum specimens) on presumptive TB patients, BioFM-Filter smear exhibited a significant increase in positivity of 7% and 4% over ZN smear and LED-FM smear (p

Published
2019
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2. Development and evaluation of novel bio-safe filter paper-based kits for sputum microscopy and transport to directly detect Mycobacterium tuberculosis and associated drug resistance

Author

Rohit Sarin, Nalini Gupta, Rakesh Kumar Gupta, Hitesh Verma, Sagarika Haldar, Vithal Prasad Myneedu, Divya Anthwal, Jaya Sivaswami Tyagi, Prem Prakash Sharma, Ashawant Gupta, Viveksheel Malhotra, Ajoy Kumar Verma, and Surabhi Lavania

Subjects
0301 basic medicine, Bacterial Diseases, Physiology, Molecular biology, Extensively Drug-Resistant Tuberculosis, Antitubercular Agents, Drug resistance, Molecular biology assays and analysis techniques, Biochemistry, 0302 clinical medicine, Drug Resistance, Multiple, Bacterial, Tuberculosis, Multidrug-Resistant, Medicine and Health Sciences, 030212 general & internal medicine, DNA extraction, Multidisciplinary, biology, Isoniazid, Body Fluids, Actinobacteria, Infectious Diseases, Medicine, Tuberculosis Diagnosis and Management, medicine.symptom, Anatomy, Rifampin, medicine.drug, Research Article, Fluoroquinolones, medicine.medical_specialty, Tuberculosis, Science, 030106 microbiology, India, Mycobacterium tuberculosis, 03 medical and health sciences, Extraction techniques, Diagnostic Medicine, Internal medicine, medicine, Humans, DNA filter assay, Bacteria, business.industry, Sputum, Organisms, Extensively drug-resistant tuberculosis, Biology and Life Sciences, Biological Transport, medicine.disease, biology.organism_classification, Tropical Diseases, Research and analysis methods, Mucus, Metabolism, Molecular biology techniques, Microscopy, Fluorescence, Reagent Kits, Diagnostic, business, Rifampicin
Abstract

India has the highest burden of Tuberculosis (TB) and multidrug-resistant TB (MDR-TB) worldwide. Innovative technology is the need of the hour to identify these cases that remain either undiagnosed or inadequately diagnosed due to the unavailability of appropriate tools at primary healthcare settings. We developed and evaluated 3 kits, namely ‘TB Detect’ (containing BioFM-Filter device), ‘TB Concentration and Transport’ (containing Trans-Filter device) and ‘TB DNA Extraction’ kits. These kits enable bio-safe equipment-free concentration of sputum on filters and improved fluorescence microscopy at primary healthcare centres, ambient temperature transport of dried inactivated sputum filters to central laboratories and molecular detection of drug resistance by PCR and DNA sequencing (Mol-DST). In a 2-site evaluation (n = 1190 sputum specimens) on presumptive TB patients, BioFM-Filter smear exhibited a significant increase in positivity of 7% and 4% over ZN smear and LED-FM smear (p

Published
2019

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