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23 results on '"Vongchan, Preeyanat"'

8. Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

Author

Areewong, Chanakan, primary, Rittipornlertrak, Amarin, additional, Nambooppha, Boondarika, additional, Fhaikrue, Itsarapan, additional, Singhla, Tawatchai, additional, Sodarat, Chollada, additional, Prachasilchai, Worapat, additional, Vongchan, Preeyanat, additional, and Sthitmatee, Nattawooti, additional

Published
2020
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9. Associations among tourist camp management, high and low tourist seasons, and welfare factors in female Asian elephants in Thailand

Author

Norkaew, Treepradab, primary, Brown, Janine L., additional, Thitaram, Chatchote, additional, Bansiddhi, Pakkanut, additional, Somgird, Chaleamchat, additional, Punyapornwithaya, Veerasak, additional, Punturee, Khanittha, additional, Vongchan, Preeyanat, additional, Somboon, Nopphamas, additional, and Khonmee, Jaruwan, additional

Published
2019
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10. Influence of season, tourist activities and camp management on body condition, testicular and adrenal steroids, lipid profiles, and metabolic status in captive Asian elephant bulls in Thailand

Author

Norkaew, Treepradab, primary, Brown, Janine L., additional, Bansiddhi, Pakkanut, additional, Somgird, Chaleamchat, additional, Thitaram, Chatchote, additional, Punyapornwithaya, Veerasak, additional, Punturee, Khanittha, additional, Vongchan, Preeyanat, additional, Somboon, Nopphamas, additional, and Khonmee, Jaruwan, additional

Published
2019
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11. Production and characterization of polyclonal antibody against Bengal tiger (Panthera tigris tigris) immunoglobulin G

Author

Areewong, Chanakan, primary, Sangchantip, Rinrada, additional, Rungphattanachaikul, Surapa, additional, Rittipornlertrak, Amarin, additional, Fhaikruae, Itsarapan, additional, Wongkalasin, Waroot, additional, Nomsiri, Ruksiri, additional, Boontong, Pinich, additional, Vongchan, Preeyanat, additional, and Sthitmatee, Nattawooti, additional

Published
2019
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12. Influence of season, tourist activities and camp management on body condition, testicular and adrenal steroids, lipid profiles, and metabolic status in captive bull elephants in Thailand

Author

Khonmee, Jaruwan, primary, Norkaew, Treepradab, additional, Brown, Janine L., additional, Bansiddhi, Pakkanut, additional, Somgird, Chaleamchat, additional, Thitaram, Chatchote, additional, Punyapornwithaya, Veerasak, additional, Punturee, Khanittha, additional, Vongchan, Preeyanat, additional, and Somboon, Nopphamas, additional

Published
2018
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13. Body condition and adrenal glucocorticoid activity affects metabolic marker and lipid profiles in captive female elephants in Thailand

Author

Norkaew, Treepradab, primary, Brown, Janine L., additional, Bansiddhi, Pakkanut, additional, Somgird, Chaleamchat, additional, Thitaram, Chatchote, additional, Punyapornwithaya, Veerasak, additional, Punturee, Khanittha, additional, Vongchan, Preeyanat, additional, Somboon, Nopphamas, additional, and Khonmee, Jaruwan, additional

Published
2018
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21. Thalidomide Stimulates T Cell Responses and Interleukin 12 Production in HIV-Infected Patients

Author

Haslett, Patrick A.J., primary, Klausner, Jeffrey D., additional, Makonkawkeyoon, Sanit, additional, Moreira, Andre, additional, Metatratip, Prasit, additional, Boyle, Brian, additional, Kunachiwa, Warunee, additional, Maneekarn, Niwat, additional, Vongchan, Preeyanat, additional, Corral, Laura G., additional, Elbeik, Tarek, additional, Shen, Zhu, additional, and Kaplan, Gilla, additional

Published
1999
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22. Inhibition of human tumor xenograft growth in nude mice by a novel monoclonal anti-HSPG isolated from human liver.

Author

Vongchan P, Kothan S, Wutti-In Y, and Linhardt RJ

Subjects
Animals, Antibodies, Monoclonal chemistry, Apoptosis, Cell Line, Tumor, Cell Proliferation, Hep G2 Cells, Humans, Immunohistochemistry methods, Immunotherapy methods, Liver pathology, Liver Neoplasms metabolism, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Transplantation, Proteoglycans chemistry, Tetrazolium Salts pharmacology, Thiazoles pharmacology, Heparan Sulfate Proteoglycans immunology, Liver metabolism, Liver Neoplasms therapy
Abstract

Heparan sulfate proteoglycans (HSPGs) were isolated from normal human liver and α monoclonal antibody (MAb) was raised against them. Preliminary studies showed that MAb clone 1E4-1C2 was able to react with many cell lines tested, including hematopoietic cells and solid tumors. MAb1E4-1C2 was used to study whether HSPG was involved in growth and proliferation of human liver cancer using hepatocellular carcinoma (HCC) cell line (HepG2) as a model. Inhibition by MAb1E4-1C2 of HepG2 cell proliferation was studied in vitro by MTT assay. For in vivo assay, xenograft induction in athymic mice was performed. The results showed that MAb1E4-1C2 inhibited proliferation of HepG2 cells significantly, compared to isotype and medium control. MAb1E4-1C2 also suppressed the growth of tumor, resulting in smaller tumor size and weight. The investigation also showed that MAb1E4-1C2 inhibited proliferation and restricted tumor growth through the induction of apoptosis. The results suggest that HSPG might be involved in liver cancer cell proliferation. Therefore, a specific MAb that was raised against liver HSPG might be an alternative therapeutic agent for the treatment of human liver cancer.

Published
2011

23. Production of a monoclonal antibody against a yeast secreted antigen of Penicillium marneffei.

Author

Pornprasert S, Dettrairat S, Vongchan P, and Apichatpiyakul C

Subjects
Animals, Antibodies, Monoclonal biosynthesis, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Mice, Inbred BALB C, Mycoses microbiology, Penicillium isolation & purification, Rabbits, Sensitivity and Specificity, Serologic Tests, Thailand, Yeasts growth & development, Antibodies, Monoclonal immunology, Antigens, Fungal immunology, Mycoses diagnosis, Penicillium immunology, Yeasts immunology
Abstract

Monoclonal antibody against P. mameffei yeast secreted antigen was produced in order to develop a serological test for penicilliosis marneffei. The yeast form of P. marneffei was cultured in brain heart infusion broth at 37 degrees C for 7 days. A secreted antigen was prepared, partially purified from culture supernatant and subsequently immunized in a BALB/c mouse. Mouse monoclonal antibody was produced from immune spleen cells by a standard hybridoma technique. Specificity of the obtained monoclonal antibody was assessed with yeast secreted antigens for P. mameffei, C. alblicans, C. neoformans, and H. capsulatum by an indirect ELISA. Three of 46 hybrid clones (1 F1, 2G5, and 3G4) reacted positively with P mameffei secreted antigen. 1 F1 and 3G4 were cloned by two rounds of limiting dilution. Partially purified monoclonal antibody and rabbit polyclonal antibody against P. marneffei yeast secreted antigen were used to develop a double antibody sandwich ELISA to detect P. marneffei antigen in plasma or serum samples of 7 patients with penicilliosis marneffei and 5 healthy controls. The sandwich ELISA developed using monoclonal antibody as a capture antibody and rabbit polyclonal antibody as a detector was able to detect P. marneffei antigen in all the plasma and serum samples of penicilliosis marneffei patients, while negative in all the healthy controls. Thus, the monoclonal antibody produced in the present study appeared to be highly specific for P. marneffei and the double antibody sandwich ELISA developed using monoclonal and polyclonal antibodies against the yeast secreted antigen of P. marneffei showed a strong potential for the diagnosis of penicilliosis marneffei.

Published
2005

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