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Your search keyword '"Vorsanova, S. G."' showing total 204 results
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204 results on '"Vorsanova, S. G."'

20. Combination of atomic force microscopy and mass spectrometry for the target protein in the serum samples of children with autism spectrum disorders

Author

Kaysheva, A. L., Pleshakova, T. O., Kopylov, A. T., Shumov, I. D., Iourov, I. Y., Vorsanova, S. G., Yurov, Y. B., Izotov, A. A., Archakov, A. I., and Ivanov, Y. D.

Abstract

Показана возможность детекции целевых белков, ассоциированных с развитием аутистических расстройств у детей, с помощью комбинированного метода АСМ/МС. Метод основан на комбинации аффинного обогащения белков из биообразцов, их АСМ-визуализации и МС-анализе с количественной детекцией целевых белков. Possibility of detection of target proteins associated with development of autistic disorders in children with use of combined AFM/MS method is demonstrated. The proposed method is based on the combination of affine enrichment of proteins from biological samples, visualization of these proteins by AFM and MS analysis with quantitative detection of target proteins. Работа выполнена при поддержке гранта РНФ 14-25-00132. Шумов И.Д. является получателем стипендии Президента РФ молодым ученым и аспирантам, осуществляющим перспективные научные исследования и разработки по приоритетным направлениям модернизации российской экономики на 2016-2018 годы (СП-4280.2016.4).

Published
2017

21. Комбинация атомно-силовой микроскопии и масс-спектрометрии для регистрации целевых белков в образцах сыворотки крови детей с расстройствами аутистического спектра

Author

Кайшева, А. Л., Плешакова, Т. О., Копылов, А. Т., Шумов, И. Д., Юров, И. Ю., Ворсанова, С. Г., Юров, Ю. Б., Изотов, А. А., Арчаков, А. И., Иванов, Ю. Д., Kaysheva, A. L., Pleshakova, T. O., Kopylov, A. T., Shumov, I. D., Iourov, I. Y., Vorsanova, S. G., Yurov, Y. B., Izotov, A. A., Archakov, A. I., Ivanov, Y. D., Кайшева, А. Л., Плешакова, Т. О., Копылов, А. Т., Шумов, И. Д., Юров, И. Ю., Ворсанова, С. Г., Юров, Ю. Б., Изотов, А. А., Арчаков, А. И., Иванов, Ю. Д., Kaysheva, A. L., Pleshakova, T. O., Kopylov, A. T., Shumov, I. D., Iourov, I. Y., Vorsanova, S. G., Yurov, Y. B., Izotov, A. A., Archakov, A. I., and Ivanov, Y. D.

Abstract

Показана возможность детекции целевых белков, ассоциированных с развитием аутистических расстройств у детей, с помощью комбинированного метода АСМ/МС. Метод основан на комбинации аффинного обогащения белков из биообразцов, их АСМ-визуализации и МС-анализе с количественной детекцией целевых белков., Possibility of detection of target proteins associated with development of autistic disorders in children with use of combined AFM/MS method is demonstrated. The proposed method is based on the combination of affine enrichment of proteins from biological samples, visualization of these proteins by AFM and MS analysis with quantitative detection of target proteins.

Published
2017

29. Brain tissue preparations for chromosomal PRINS labeling

Author

Iourov, I. Y., Vorsanova, S. G., Pellestor, F., Yuri Yurov, Institut de génétique humaine ( IGH ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de génétique humaine (IGH), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Adult, Cell Nucleus, Quality Control, [SDV.GEN]Life Sciences [q-bio]/Genetics, Paraffin Embedding, Tissue Fixation, Primed In Situ Labeling, Brain, Fetus, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Formaldehyde, Chromosomes, Human, Humans, [ SDV.GEN ] Life Sciences [q-bio]/Genetics, [ SDV.GEN.GH ] Life Sciences [q-bio]/Genetics/Human genetics, Interphase, Cells, Cultured
Abstract

The preparation of a somatic cell tissue is a key point of any successful molecular biology and genetic analysis of chromosome complement and genome variations. Current molecular biology investigations require an increased amount of different tissues to study. The genetic studies of the brain became an intriguing part of oncology, neurogenetics, and psychiatric genetics. The brain tissue processing for molecular cytogenetic analysis has special peculiarities quite different from the protocols used in the field. Despite some pilot cytogenetic studies of chromosome complement in mammalian brain, the specified protocol of the brain sample preparation for molecular cytogenetic analysis has not been thoroughly described. Here, we propose the detailed brain tissue processing protocol that could be used for different molecular cytogenetic approaches to study chromosome complement in interphase nuclei.

Published
2006

34. Trisomy 21 Mosaicism: We May All Have a Touch of Down Syndrome

Author

Hulten, M A., Jonasson, Jon, Iwarsson, E, Uppal, P, Vorsanova, S G., Yurov, Y B., Iourov, I Y., Hulten, M A., Jonasson, Jon, Iwarsson, E, Uppal, P, Vorsanova, S G., Yurov, Y B., and Iourov, I Y.

Abstract

Ever increasing sophistication in the application of new analytical technology has revealed that our genomes are much more fluid than was contemplated only a few years ago. More specifically, this concerns interindividual variation in copy number (CNV) of structural chromosome aberrations, i.e. microdeletions and microduplications. It is important to recognize that in this context, we still lack basic knowledge on the impact of the CNV in normal cells from individual tissues, including that of whole chromosomes (aneuploidy). Here, we highlight this challenge by the example of the very first chromosome aberration identified in the human genome, i.e. an extra chromosome 21 (trisomy 21, T21), which is causative of Down syndrome (DS). We consider it likely that most, if not all, of us are T21 mosaics, i.e. everyone carries some cells with an extra chromosome 21, in some tissues. In other words, we may all have a touch of DS. We further propose that the occurrence of such tissue-specific T21 mosaicism may have important ramifications for the understanding of the pathogenesis, prognosis and treatment of medical problems shared between people with DS and those in the general non-DS population., Funding Agencies|Wellcome Trust|061202/ZOOZ|BBSRC|BB/C003500/1|Swedish Research Council||Stockholm County Council||Deutsches Luft- und Raumfahrtszentrum/Bundesministerium fur Bildung und Forschung|RUS 09/006RUS 11/002

Published
2013
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38. Dynamic mosaicism manifesting as loss, gain and rearrangement of an isodicentric Y chromosome in a male child with growth retardation and abnormal external genitalia.

Author

Iourov, I. Y, Vorsanova, S. G., Liehr, I. V., Monakhov, Y. B., Soloviev, and Yurov

Subjects
*GENETICS, *CHROMOSOMES, *Y chromosome, *SEX chromosomes, *CELL culture
Abstract

Isodicentric chromosomes are considered the most common structural abnormality of the human Y chromosome. Because of their instability during cell division, loss of an isodicentric Y seems mainly to lie at the origin of mosaicism in previously reported patients with a 45,X cell line. Here, we report on a similar case, which, however, turned out to be an example of dynamic mosaicism involving isodicentric chromosome Y and isochromosome Y after FISH with a set of chromosome Y-specific probes and multicolor banding. Cytogenetic analyses (GTG-, C-, and Q-banding) have shown three different cell lines: 45,X/46, X,idic(Y)(q12)/46,X,+mar. The application of molecular cytogenetic techniques established the presence of four cell lines: 45,X (48%), 46,X,idic(Y)(q11.23) (42%), 46,X,i(Y)(p10) (6%) and 47,X,idic(Y)(q11.23),+idic(Y)(q11.23) (4%). According to the available literature, this is the first case of dynamic mosaicism with up to four different cell lines involving loss, gain, and rearrangement of an idic(Y)(q11.23). The present report indicates that cases of mosaicism involving isodicentric and isochromosome Ys can be more dynamic in terms of somatic intercellular variability that probably has an underappreciated effect on the phenotype. Copyright © 2008 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Published
2008
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40. Intercellular Genomic (Chromosomal) Variations Resulting in Somatic Mosaicism: Mechanisms and Consequences

Author

Iourov, I. Y., Vorsanova, S. G., and Yurov, Y. B.

Abstract

Large-scale variations of the human genome can be produced by losses or gains of whole chromosomes (aneuploidy). In contrast to DNA sequences variations at subchromosomal level (single nucleotide polymorphisms, short tandem repeat variations) or interindividual subtle chromosome region changes (deletions, duplications, large-scale copynumber variants, fragile sites), aneuploidy simultaneously involves hundreds or even thousands of genes and, therefore, dramatically affects functional genome activity. Aneuploidy originates from either meiotic or mitotic chromosome instability and, in some instances, manifests as somatic chromosomal mosaicism. Although the real incidence of mosaic aneuploidy in somatic human tissues remains to be determined, one can suppose an overlooked fraction of cells with unshared genomes due to large-scale genomic alterations among 1014 cells forming the human body. Intercellular differences in chromosome number can be considered an overlooked type of structural and functional genome variations, which produce genetic mosaicism. This review refers to somatic chromosomal mosaicism and aims to describe its mechanisms and consequences. Moreover, the effect of somatic chromosomal mosaicism on both interindividual and intercellular diversity as well as human diseases is discussed. Finally, since the identification of these genomic variations faces numerous difficulties, we found pertinent to describe available approaches towards the detection of chromosomal mosaicism in human somatic tissues.

Published
2006

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