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387 results on '"Vossoughi, Manouchehr"'

11. Investigation of encapsulation of pancreatic beta cells and curcumin within alginate microcapsules.

Author

Hosseinzadeh, Zahra, Alemzadeh, Iran, and Vossoughi, Manouchehr

Subjects
PANCREATIC beta cells, ALGINIC acid, CURCUMIN, TYPE 1 diabetes, INSULIN, BETA functions, CELL survival
Abstract

Cell encapsulation is an ideal approach for the replacement of pancreatic function in Type 1 diabetes. Poor biocompatibility of microcapsules generates an inflammatory response in the implantation site and induces fibrosis infiltration, which causes microencapsulated cell death and graft failure. To prevent inflammation after implantation, composite microcapsules that exhibit anti‐inflammatory properties were designed. This study is about encapsulating beta cells and curcumin within 1.5% alginate by the jet‐breaking regime of the syringe pump. The microcapsules' size distribution and rate of the alginate solution were characterized to find uniform particles. Micro‐size particles were attained at a rate of 25 mL/min. Uniform spherical microcapsules (200–300 μm) were created in large amounts in a short period. Microcapsule breakage was less than 3% during 7 days, which demonstrated the stability of the encapsulation method. Insulin secretion and cell viability assays were performed 1, 3, and 7 days after microencapsulation by glucose‐stimulated insulin secretion (GSIS) and 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyl tetrazolium bromide (MTT) assays. No significant differences in the amount of insulin secretion and beta cell viability were observed among free cells, alginate microcapsules, and curcumin‐alginate microcapsules during 7 days (p > 0.05). Therefore, the curcumin and alginate membrane did not show any harmful impacts on the function and survival of the beta cells. [ABSTRACT FROM AUTHOR]

Published
2024
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47. Magnetic labelled HRP-polymer nanoparticles: A recyclable nanobiocatalyst

Author

Khosravi Arezoo, Vossoughi Manouchehr, Shahrokhian Saeed, and Alemzadeh Iran

Subjects
Horseradish peroxidase, nano-reengineered enzyme, magnetic nanoparticles, recyclable biocatalyst, Chemistry, QD1-999
Abstract

In this paper, the reusability and process stability of nano-reengineered horseradish peroxidase was investigated in fluorescence based sensing system for hydrogen peroxide determination as a model application. To this end, dendron macromolecules were attached to enzyme surface through bio-conjugation techniques. The resulted enzyme-polymer nanoparticles, with average size of 14(±2) nm, showed significant life time and thermal stability. For enzyme recovery and reusability purposes, the enzyme-polymer nanoparticles were labelled with magnetic nanoparticles with a labelling yield of 90%. These labelled enzyme molecules showed significant process stability up to 7 recycling period in a model sensing system. A linear calibration curve was obtained over the hydrogen peroxide concentrations ranging from 5×10-8 mol L-1 to 1×10-5 mol L-1 with detection limit of 1.3×10-9 mol L-1 for the sensing system under the optimal conditions.

Published
2013
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