Search

AMAÇ: İleri yaş erkeklerde sık görülmekte olan prostat ve böbrek hastalıklarının tarama, tanı ve tedavi takiplerinde kullanılmakta olan prostat spesifik antijen (PSA) ve kreatinin, tahmini glomerüler filtrasyon hızı (eGFR), üre ürik asit düzeyleri arasındaki ilişkilerin araştırılması amaçlandı. GEREÇ VE YÖNTEM: Eylül 2017 – Mart 2020 tarihleri arasında 40 yaş ve üzerindeki 7203 erişkin erkek hastaya ait eş zamanlı olarak istenmiş olan serum PSA, kreatinin, üre ve ürik asit sonuçları retrospektif olarak incelendi. Parametreler arasındaki korelasyonlar yaşın etkisi ile birlikte değerlendirildi. Ek olarak PSA yüksekliği ile eGFR azalışı arasındaki çift yönlü ilişki de incelendi. BULGULAR: Yaş değişkeni kontrol edildiğinde; PSA düzeyleri üzerine kreatinin, üre ve ürik asit düzeylerinin bir etkisi olmadığı (sırasıyla; p=0,63, p=0,28 ve p=0,39), benzer şekilde PSA düzeylerinin de kreatinin, üre ve ürik asit düzeyleri üzerine bir etkisi olmadığı gözlendi (sırasıyla; p=0,22, p=0,25 ve p=0,92). Yaş değişkeni ile birlikte yapılan regresyon analizlerinde; yaş arttıkça PSA yüksekliği görülme riski 1.09 kat artmakta iken kreatinin arttıkça ise riskin anlamlı bir değişim göstermediği tespit edildi (sırasıyla; p

BACKGROUND: Cesarean delivery (CD) is the most frequently performed surgery. Surgical site infection (SSI) is a common complication after CD. PURPOSE: To evaluate the role of complete blood count parameters in predicting the development of SSI after CD. METHODS: Patients who were hospitalized because of SSI after CD were included in the SSI group (n = 48). A control group (n = 45) was formed with healthy postpartum women who had also undergone CD. Preoperative and postoperative hemoglobin (Hb), white blood cell count (WBC), neutrophil count, lymphocyte count, platelet count (PLT), neutrophil-to-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), mean platelet volume (MPV), and platelet-large cell ratio (P-LCR) were recorded. RESULTS: WBC, neutrophil count, and NLR levels increased while Hb, PLT, and lymphocyte count levels decreased after CD in both the SSI and control groups. PLR increased after CD in the SSI group but remained stable in the control group. However, the difference in PLR could not predict SSI after CD. CONCLUSION: Complete blood count parameters were not useful to predict SSI after CD. Larger prospective studies are needed.

Residency training programs are challenging for young physicians with heavy workloads. Although ultrasonography (USG) is an imaging method that is frequently used in obstetrics practice, some basic USG skills can be acquired late in this intensive learning process. Likewise determining the fetal heart axis is an elementary evaluation but can turn into a challenging and time-consuming process, especially for inexperienced clinicians.Pregnant women between 20 and 37 weeks of gestation were recruited. Two observers assessed the axis of fetal heart by standard, Bronshtein and clock position methods. Fetal heart axis evaluation times were compared. Inter-observer and intra-observer agreements of the three methods were measured. One factor learning rates were calculated.A total of 31 pregnant patients between the ages of 18 and 40 years were included in the study. Fetal heart axis evaluation time by the clock position method was shorter than the Bronshtein and standard method in both observers. Furthermore diagnostic accuracy for both observers was 100% with the clock position method, while this fell to 100% in observer-1 and 96.8% in observer-2 using the Bronshtein method. The clock position method was learned faster than either of the other methods.Clock position method is an easy and feasible method for inexperienced resident physicians in terms of learning and application to determine the fetal heart axis. The advantages of this method increase when patient numbers are higher.

We aimed to examine the relationship between laboratory markers and the severity of the disease in pregnant women diagnosed with coronavirus disease 2019 (COVID-19). Clinical records were retrospectively reviewed for 112 pregnant women. Patients diagnosed with COVID-19 were divided into two groups as mild/moderate and severe. The relationship between predicting the severity of the disease and laboratory parameters was investigated. Neutrophil lymphocyte ratio, C-reactive protein (CRP), ferritin and aspartate aminotransferase levels were significantly higher in severe COVID-19 cases than mild/moderate cases (

Background Various studies have reported different results for cortisol, dehydroepiandrosterone sulfate (DHEA-S) and 25-hydroxyvitamin D (25(OH)D) levels in patients with chronic spontaneous urticaria (CSU) and these were not sufficient for explaining the underlying reasons. Objectives To evaluate the levels of cortisol, DHEA-S and 25(OH)D in patients with CSU and to investigate the relationships between these parameters. Methods Fifty patients who had diagnosed with CSU and 30 healthy controls were enrolled into the study. Stress levels of CSU and control groups were determined by perceived stress scale (PSS-14). The activity of urticaria of the patients was also determined by urticaria activity score (UAS7). Serum DHEA-S, cortisol and 25(OH)D levels of the participants were measured and compared. Results DHEA-S and 25(OH)D levels of CSU patients were lower than the control group (p Conclusions Lower 25(OH)D levels and higher cortisol/DHEA-S ratio in CSU patients who have higher stress level indicate that the level of 25(OH)D seems to be associated with steroidogenesis and thus 25(OH)D levels may decrease secondarily in CSU.

Amaç: Tiroid stimülan hormon (TSH), serbest T4 (sT4) ve serbest T3 (sT3) testlerinin referans aralıklarını laboratuvarımızın sonuç verilerinden indirekt yöntem ile belirlemek ve belirlenen referans aralıkları kullanmakta olduğumuz kitlerin ve yöntemin üreticisi tarafından önerilen referans aralıklarla karşılaştırmaktır. Gereç ve Yöntem: Ocak – Ekim 2021 tarihleri arasında laboratuvarımızda çalışılmış olan 1-99 yaş aralığındaki 195205 kişiye ait eş zamanlı olarak istenen TSH, sT4 ve sT3 sonuçları çalışmaya dahil edildi. Uç değerlerin belirlenmesi ve dışlanmasında Tukey yöntemi kullanıldı. Ardından non-parametrik ve Bhattacharya yöntemleri ile TSH, sT4 ve sT3 referans aralıkları hesaplandı. Referans aralıklar arasındaki uyumluluğun değerlendirilmesinde CLIA 19 kabul edilebilir limitleri kullanıldı. Bulgular: Pediatrik yaş grubu (1-17 yaş) için referans aralıklar non-parametrik yöntem ile TSH (0,56 - 5,93 mIU/L), sT4 (8,9 - 16,1 ng/L) ve sT3 (2,74 - 5,39 ng/L) bulunurken; Bhattacharya yöntemi ile TSH (0,67 - 3,08 mIU/L), sT4 (9,2 - 16,1 ng/L) ve sT3 (2,72 - 5,46 ng/L) olarak bulundu. Erişkin yaş grubu (18-99 yaş) için referans aralıklar non-parametrik yöntem ile TSH (0,10 - 4,98 mIU/L), sT4 (8,7 - 16,1 ng/L) ve sT3 (2,01 - 4,09 ng/L) bulunurken; Bhattacharya yöntemi ile TSH (0,18 - 3,07 mIU/L), sT4 (9,1 - 16,2 ng/L) ve sT3 (2,05 - 4,13 ng/L) olarak bulundu. Sonuç: sT4 ve sT3 referans aralıklarımızın üreticinin referans aralıkları ile uyumlu olarak kabul edilebilir olduğunu ancak TSH testi için pediatrik gruptaki üst referans sınır ve erişkin gruptaki alt referans sınır ile üreticinin referans sınırları arasında farklılık olduğunu belirledik. Laboratuvarların kendi referans aralıklarını belirlemesinin, test sonuçlarının doğru yorumlanabilmesi açısından büyük önem taşıdığını düşünmekteyiz.

Clusterin is related to immunity and inflammation via regulation of complement activation and bidirectional regulation, and by major proinflammatory cytokines. Clusterin levels have been the subject of a few research both in patients with hyperlipidemia and those with chronic spontaneous urticaria (CSU) separately. The aims of this study were to evaluate the levels of clusterin levels and serum lipids and the relationships between them in patients with CSU.Fifty patients with CSU and 30 healthy controls were enrolled into the study. The activity of urticaria of the patients was determined by urticaria activity score (UAS7). Serum clusterin, total cholesterol, HDL, LDL and triglyceride levels of the participants were measured and compared. The relationships between UAS, lipids and clusterin were examined.There was no difference in clusterin levels between CSU patients and controls. Clusterin level was not related to activity of urticaria. Clusterin levels were not correlated with any of lipid parameters neither in CSU patients nor in controls.Findings of this study show that clusterin levels do not change due to CSU. Serum clusterin levels cannot be used as a diagnostic or a disease activity marker in CSU patients, regardless of the lipid profile.

Hypertension (HT) in obesity has been reported frequently in children in recent years. The role of copeptin and uric acid here are not well known. We aimed to investigate the relationship between HT and serum copeptin and uric acid levels in children with obesity.We included 80 children with obesity who were admitted to our hospital between April 2018 and September 2018. The patients were separated into two groups: hypertensive and non-hypertensive. Serum copeptin levels were measured using the enzyme-linked immunosorbent assay.Copeptin levels were significantly higher in patients with HT than in those without (p = 0.0001). In addition, serum uric acid levels in patients with HT were significantly higher, while the serum potassium levels were significantly lower (p = 0.01) than in those without HT (p = 0.001). In correlation analyses, a positive correlation was detected between blood sodium and copeptin levels (p = 0.037).Hypertensive children with obesity had higher serum copeptin and uric acid and lower blood potassium levels. Moreover, copeptin levels were positively correlated with blood sodium levels. Thus, in addition to copeptin, serum uric acid, potassium, and sodium levels may be important in the diagnosis and follow-up of children with HT.

Purpose: Psoriasis is a chronic, inflammatory, and systemic disease. The disease activity is usually measured by Psoriasis Area and Severity Index (PASI), however, further objective laboratory tools are needed. Neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR) and mean platelet volume (MPV) are objectively calculated markers that increase in inflammation. This study aimed to investigate the utility of these markers for follow-up of systemic inflammation and response to treatment. Material and methods: A total of 25 patients who had moderate or severe psoriasis vulgaris, who received ustekinumab treatment were retrospectively evaluated in the study. In patients, pre-treatment PASI, complete blood count parameters, serum CRP levels and NLR, PLR and MPV values in the follow up when they achieved PASI 75 and/or more improvement were recorded. Patients with an additional inflammatory disease or accompanying infection were excluded from the study. Results: While a statistically significant decline was recorded in NLR and PLR (p, Giriş: Psoriazis kronik, inflamatuar ve sistemik bir hastalıktır. Psoriaziste hastalık şiddetini ölçmek için Psoriazis Alan Şiddet İndeksi (PAŞİ) yanı sıra daha objektif laboratuar araçlarına ihtiyaç vardır. Nötrofil lenfosit oranı (NLO), trombosit lenfosit oranı (TLO), ve oratalama trombosit hacmi (OTH) çeşitli inflamatuar hastalıklarda arttığı bilinen belirteçlerdir. Bu çalışmada amacımız orta şiddette ve şiddetli psoriasis vulgarisi olan hastalarda NLO, TLO ve OTH değerlerinin ustekinumab tedavisi ile değişimlerini araştırmaktır.Gereç ve yöntem: Çalışmada orta şiddette veya şiddetli psoriazis vulgarisi olup, ustekinumab tedavisi verilen kronik plak tipi psoriasis tanılı 25 hasta retrospektif olarak değerlendirilmiştir. Hastaların tedavi öncesi PASI, tam kan sayımı parametreleri, serum CRP seviyeleri ve PAŞİ 75 ve/veya üzeri başarı elde edildikleri kontrollerindeki değerleri kaydedilmiştir. Tam kan sayımı belirteçleri, OTH ve CRP değeri determine edilmiştir. Ek inflamatuar hastalığı ve eşlik eden enfeksiyonu olanlar çalışma dışı bırakılmıştır.Bulgular: Hastaların PASI 75 başarı ve üzeri elde ettikleri incelemelerde NLO, TLO oranlarında istatistiksel olarak anlamlı düşme (p

Objective:We aimed to contribute to the literature by determining deflection point of parathyroid hormone (PTH) level compared to 25-hydroxy vitamin D3(25(OH)D3) level for determining the cut-off value of vitamin D deficiency level in Turkish adults.Methods:The levels of 25(OH)D3and intact parathyroid hormone (iPTH) which were requested simultaneously in 1 year of 1684 adults were evaluated retrospectively. 25(OH)D3levels were first classified as 80–50, 50–30, 30–25, 25–20, 20–15, 15–10, 10–5, 5–0 ng/mL and iPTH levels among those groups were compared.Results:First significant difference in iPTH levels was determined between 25(OH)D3groups of 80–50 and 50–30 ng/mL (p=0.007). Second and third significant differences were determined between 25(OH)D3groups of 15–10 and 10–5 ng/mL and between 10–5 and 5–0 ng/mL, respectively (p=0.006 and p=0.035, respectively). There were no differences in iPTH levels among seasons (p=0.11).Conclusion:In the light of these findings; we can state that iPTH levels are suppressed when 25(OH)D3over 50 ng/mL, remains stable when 25(OH)D3levels in between 50–10 ng/mL and gives the first increase response when 25(OH)D3falls below 10 ng/mL. We believe that cut-off value for vitamin D deficiency in Turkish adults at all seasons depending on PTH response should be used as 10 ng/mL.

Amaç: Bu çalışmada, inflamasyona yanıt olarak hepsidin sentezindeki değişimin değerlendirilmesi ve inflamatuar bir hastalık olan Behçet hastalığında (BH) hepsidin ve akut faz reaktanları arasındaki ilişkiyi ortaya koymak amaçlandı. Gereç ve Yöntemler: Çalışmaya 52 BH (15 aktif, 37 inaktif hasta) 13 izole demir eksikliği anemisi hastası, 13 sağlıklı kontrol, toplamda 78 katılımcı dâhil edildi. Kronik hastalık anemili hastaların 3'ü aktif, 1'i inaktif behçet hastasıydı. ELISA yöntemiyle biyoaktif hepsidin-25 düzeylerini ölçüldü.Bulgular: İnaktif BH ve ana BH gruplarında sağlıklı kontrollere göre anlamlı derecede düşük hepsidin düzeyleri saptandı (sırasıyla p=0.015, p=0.014); hepsidin düzeylerini, kronik hastalık anemisi olan BH grubu ile sağlıklı kontroller ve izole demir eksikliği anemisi grubu karşılaştırıldığında ve istatistiksel olarak anlamlı sonuç elde edildi. (sırasıyla p=0.007, p=0.003). Ayrıca aktif BD grubunun hepcidin düzeyleri ile sağlıklı kontroller arasında anlamlı bir fark bulunamadı (p> 0.05).Sonuç: İnflamasyondaki artış paterninin aksine, Behçet hastalarında artmış hepsidin seviyelerini tespit edilmedi. Hepsidin seviyelerini etkileyen antiinflamatuar tedavilerin serum İnterlökin-6 (IL-6) düzeylerini değiştirdiğinden, daha önce teşhis edilmiş ve/veya daha önce tedavi almayan Behçet hastalarının IL-6 düzeyleri ile birlikte hepsidin düzeylerinin ölçümü ve değerlendirilmesi gerekmektedir., Aim: This study aims to evaluate the alteration of hepcidin synthesis in response to inflammation and to reveal the relationship between hepcidin and acute phase reactants in Behcet’s disease (BD) which is also an inflammatory disease.Material and Methods: That was included 52 BD patients (15 with active, 37 with inactive disease), 13 isolated iron deficiency anemia patients and 13 healthy controls; totally 78 individuals to the study. Patients with chronic disease anemia had 3 active and 1 inactive Behcet's disease. Levels of bioactive hepcidin-25 were measured by ELISA method. Result: Low hepcidin levels were found significantly in inactive BD and the main BD groups comparing to healthy controls (p=0.015, p=0.014, respectively); in BD with anemia of chronic disease group comparing to healthy controls and isolated iron deficiency anemia group (p=0.007, p=0.003, respectively). Besides it was not found any significant difference between hepcidin levels of active BD group and healthy controls (p>0.05). Conclusion: Contrary to its increment pattern in inflammation, increased hepcidin levels in Behcet’s patients were not detected. Alternation of serum Interleukin-6 (IL-6) levels with anti-inflammatory treatments affecting hepcidin levels necessitate hepcidin measurement only in Behcet’s patients who have been recently diagnosed and/or haven't received a treatment before and evaluation of the measurement together with IL-6 levels.

WOS: 000412463200005 Objective: The primary aim of our study was to investigate the usefulness of serum C-reactive protein (CRP) and procalcitonin (PCT) levels in the differential diagnosis of causative gram-positive (Gram+) or gram-negative (Gram-) bacteria in patients with sepsis to facilitate decisions concerning the initial choice of an empiric antibiotic regimen. Methods: Between February 2014 and February 2016, 47 patients who had sepsis diagnosed on the basis of positive blood cultures and clinical examination results were retrospectively enrolled. Serum CRP and PCT levels of 15 gram+ and 32 gram-bacterial sepsis groups were compared using the Mann-Whitney U test. The correlation between CRP and PCT levels was calculated using the Spearman's test. Results: Among patients with bacterial sepsis, the median CRP level was 91.42 mg/L and the median PCT level was 0.46 ng/mL. There were no significant difference in CRP and PCT levels between the gram+ and gram-sepsis groups (p=0.98 and p=0.21, respectively). There was good correlation between the CRP and PCT levels (r=0.64, p

Objective:We aimed to find out whether there is significant carryover effect which causes false-positive hematuria on red blood cells (RBCs) in automatic urine chemistry (DIRUI H-800) and sediment (DIRUI FUS-200) analyzers.Methods:Twenty-four samples with gross hematuria selected as containing high RBC concentration and forty-eight samples which had both negative result in dipstick and 0/hpf in microscopic examination selected as containing low RBC concentration. Carryover% was calculated via the formula [carryover%=100×(bResults:Carryover% was very high (67%) in urine chemistry analyzer. Carryover% of urine sediment analyzer was found 0.4% whilst false-positive hematuria percentage was 87.5% for the first samples came after gross hematuria and 6.6% for the second samples. The first samples analyzed after gross hematuria had significantly higher (pConclusion:In urine sediment analyzer, carryover% calculated by formula was found analytically sufficient, but it causes highly false-positive results due to diagnostic limit of hematuria (RBC>3/hpf) is low. To prevent carryover in both urine analyzers; washing procedures should be revised and the diagnostic effect of carryover should also be taken into account by biochemists.