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1岁5月龄患儿以血常规三系减少、反复发热起病，肝脾进行性增大，骨髓病理提示造血细胞减少伴弥漫骨髓纤维化，排除恶性肿瘤等继发性原因，核型正常，无克隆异常，全外显子未检测出致病变异，未发现基因融合，诊断为原发性骨髓纤维化。脾切除术后，应用白消安、塞替哌为基础的清髓性预处理，脐带血造血干细胞移植成功。.

Acetaminophen (APAP) is a widely used antipyretic and analgesic drug that is safe and effective in the therapeutic doses, but overdose may cause hepatotoxicity and even acute liver failure (ALF). Finding reliable biomarkers for APAP toxicity is not only a hot spot of current research, but also a problem that needs to be solved urgently. Clinicians should consider the existence of APAP hepatotoxicity when using APAP treatment, and explain that APAP may have a certain degree of dose dependence. This paper reviews the most promising biomarkers currently being evaluated, and expounds their application in the field of APAP hepatotoxicity, as well as the mechanism of mitochondrial damage and mitochondrial autophagy, thereby contributing to the diagnosis, prognosis, mechanism and research progress of therapeutic targets of APAP hepatotoxicity.对乙酰氨基酚（APAP）是一种广泛应用的解热镇痛药，在治疗剂量时是安全有效的，但过量时会造成肝毒性，甚至急性肝功能衰竭。寻找可靠的APAP毒性生物标志物既是当前研究的热点，也是当前亟需解决的难题。临床医师在使用APAP治疗时，应考虑APAP肝毒性的存在，并说明APAP可能有一定程度的剂量依赖性。现对目前正在评估的生物标志物进行综述，阐述了它们在APAP肝毒性领域的应用及其肝毒性的线粒体损伤机制、线粒体自噬机制，从而有助于APAP肝毒性的诊断、预后、机制研究以及开发其治疗靶点。.

From January 2014 to June 2018, 28 patients with different types of deep soft tissue injury or infection were admitted to the Affiliated Jiangyin Hospital of Medical College of Southeast University; 5 patients were admitted to the Zhengzhou First People's Hospital. There were 24 males and 9 females, aged 18-89 (40±20) years. Disposable suction tubes with holes cut on side walls were used as self-made drainage tubes. The authors placed the self-made drainage tubes on different deep soft tissue layers and wound surfaces after debridement. The effective drainage sections of the wound surface drainage tubes were wrapped with silver ion antimicrobial functional active dressings. Bio-permeable membrane was used to close the operative area. The drainage tubes in the deep layer of wound and wound surface were connected in parallel by a tee and connected to wall-hanging medical negative-pressure suction device to conduct negative-pressure wound treatment at -20.0 to -10.6 kPa. The deep drainage tubes were usually removed or changed 4 or 5 days after surgery.The drainage tubes in the wound surface were synchronously replaced when removing or replacing he drainage tubes in the deep layer of wound. On 4 to 15 days after surgery, the deep drainage tubes were removed. On 8 to 25 days after surgery, the wound surface drainage tubes were removed. Then the treatment was changed to a conventional dressing change until the wounds were completely healed or the wound bed was ready for skin grafts or tissue flaps. The indwelling time of deep drainage tubes in this group of patients was (6.2±2.8) days, and the indwelling time of wound surface drainage tubes was (12.0±3.0) days. The wound healing time was (22±5) days, the hospital stay time was (29±7) days, and wound bacteria were reduced from 6 species and 11 strains before treatment to 3 species and 4 strains after treatment. No adverse events such as wound bleeding, irritative pain, and chronic sinus occurred during treatment. Twenty-three patients were followed up for 13 to 28 months, no treatment-related complications were observed.2014年1月—2018年6月，东南大学医学院附属江阴医院收治28例、郑州市第一人民医院收治5例不同类型深部软组织损伤或感染患者，其中男24例、女9例，年龄18～89(40±20)岁。笔者将一次性吸痰管侧壁开孔自制引流管，分别放置于清创后深部不同软组织层面和创面表层，创面表层引流管有效引流段使用银离子抗菌功能性敷料包裹，应用生物透性薄膜封闭术区，将创面深层和浅层所有引流管用三通管并联后接壁式医用负压吸引器，以－20.0～－10.6 kPa行负压伤口疗法。深层引流管一般于术后4 d或5 d拔除或更换，拔除或更换深层引流管时同步更换创面表层引流管。本组患者于术后4～15 d拔除深层引流管，术后8～25 d拔除创面表层引流管，改为常规换药，直至创面完全愈合或创基能接受皮片或组织瓣移植。本组患者创面深层引流管留置时间为(6.2±2.8)d，创面表层引流管留置时间为(12.0±3.0)d，创面愈合时间为(22±5)d，住院时间为(29±7)d，创面培养细菌由治疗前的6种11株减少为治疗后的3种4株，治疗过程中未发生创面出血、刺激性疼痛、慢性窦道形成等不良事件。23例患者随访13～28个月，未观察到与治疗相关的并发症。.

As the fixed foundation wind turbine no longer meets the design and cost requirements in deep sea power plant, and the offshore floating wind turbine (FOWT) suitable has been developed rapidly. The spar FOWT model is established in ANSYS-AQWA, and the influences of spectral peak frequency and incident angle for irregular waves on the vibration responses of FOWT are studied under five load cases. Under the guidance of similar theory, 1/150 scaled model has been simulated under typical load cases. The results show that the peak frequency of the spectrum has a significant effect on the vibration responses of FOWT, the incident angle also has a slight effect. By comparing the maximum and standard deviation (SD) of the tower top fore-aft displacement (TTDspFA) with the physical model and the experimental model, the minimum errors are 3.60% and 3.56%, respectively. This work will lay a foundation for experimental study on vibration of the floating wind turbine.

API X80 pipeline steel is widely used in natural gas and oil transportation. In this study, X80 pipeline steels with 18.4 mm thickness and 1219 mm diameter, made in China, were welded by a combined girth welding technology. Experimental results showed that microstructures in weld metal and coarse grain heat affected zone are mainly composed of coarsening bainite grains with dimensions of 20–50 μm, and Vickers hardness in weld seam is lowered by the seven-pass welding process. Simulated results illustrated that repeatedly thermal cycles imposed by the combined multipass girth welding process easily result in softened weld joints. The predicted microstructures, phase compositions and hardness in weld metal are in agreement with the measured ones. The results could be applied to optimise this combined girth welding process and improve the weld quality of API X80 pipeline steel and even higher grade pipeline steels.

Machine vision is a rapid, consistent and objective inspection technique, which has expanded into many diverse industries. Its speed and accuracy provide one alternative for an automated, non-destructive and cost-effective technique to accomplish ever-increasing production and quality requirements. This method of inspection has found applications in the agricultural industry, including the inspection and grading of fruits. The presence of skin defects is one of the most influential factors in the price of fruit, since most consumer associate quality with a good appearance and the total absence of external defects. However, defect is more difficult than colour, shape and size to be inspected and graded. In this paper, current applications of machine vision in fruit external defects detection are briefly reviewed. Some detection technologies were presented. These technologies include image processing methods based on fruit two-dimensional and three-dimensional (3D) information, and hyperspectral and...

Electroless Ni-P coatings were deposited on W-Cu composite substrates using an anodic activation and strike nickel plating pre-treatments; they were then subjected to heat treatment. The morphology and structure of Ni-P coatings were examined using SEM and XRD, also the corrosion characteristics of Ni-P coatings in 3.5 wt-% NaCl solution were investigated by electrochemical tests. Results indicated that the heat treatment temperature affected the surface morphology, structure and corrosion resistance of Ni-P coatings. The weight loss results indicated that the Ni-P coating had a smaller corrosion rate than W-Cu composite in 3.5 wt-% NaCl solution.

Electroless Ni-P coating was deposited on W-Cu alloy via potential activation at -0.8 V. The coating was characterised for its morphology, structure, microhardness and the corrosion resistance. Scanning electron microscopy observation showed the presence of smooth and compact crystals in the coating. X-ray diffraction analysis showed that the Ni-P coating was amorphous in structure. The adhesion between Ni-P coating and W-Cu alloy was qualified. Heat treatment can improve the microhardness of Ni-P coating. Both the porosity test and immersion test in 10 vol.-% H(2)SO(4) solution revealed that the Ni-P coating exhibited good corrosion resistance properties in protecting the W-Cu alloy.

To perform retrospective analysis on 20 dead cases related to heroin abuse, and to provide references for the forensic assessment of correlative cases.Among 20 dead cases related to heroin abuse, general situation, using method of drug, cause of death and result of forensic examination were analyzed by statistical analysis for summarizing the cause of death and pathologic changes.The dead were mostly young adults, with more male than female. The results of histopathological examinations showed non-specific pathological changes. There were four leading causes of death, including acute poisoning of heroin abuse or leakage （13 cases, 65%）, concurrent diseases caused by heroin abuse （3 cases, 15%）, inspiratory asphyxia caused by taking heroin （2 cases, 10%）, and heroin withdrawal syndrome （2 cases, 10%）.The forensic identification on dead related to heroin abuse must base on the comprehensive autopsy, and combine with the qualitative and quantitative analysis of heroin and its metabolites in death and the case information, as well as the scene investigation.

The humoral responses elicited in mice by inoculation, in various doses and by several routes, with plasmid DNA containing the gene coding for the circumsporozoite protein (CSP) of Plasmodium falciparum FCC1/HN were compared with those evoked by inoculation with a recombinant expressed protein based on the CSP. With the DNA vaccine, intramuscular inoculations appeared the most effective, followed by intravenous and then subcutaneous injections, the responses in each case being dose-dependent. In both standard ELISA and dot-ELISA, sera from the mice immunized with the DNA were found to have much lower titres of antimalarial antibodies than the corresponding sera from mice immunized with the recombinant protein. Although both 'vaccines' elicited humoral immune responses in BALB/c mice, that based on plasmid DNA took much longer than the recombinant protein to induce high-titre antibody responses.

Previous studies suggest that persistent post-surgical pain (PPSP) is correlated with preoperative pain status and amplification of central sensitization. Protein kinase Mζ (PKMζ) is an essential substrate of the late long-term potentiation underlying central sensitization, which is one mechanism of pain memory formation. However, the potential contributions of spinal PKMζ to PPSP, a condition in which preoperative pain is prevalent, are not known.Here, a modified 'hyperalgesia priming' model was established to simulate the clinical situation. This model used intraplantar injections of carrageenan (Car) as priming stimuli to elicit persistent nociceptive sensitization after plantar incision in rats. Upon treatment with PKMζ inhibitor ZIP, Scr-ZIP or protein kinase Cs (PKCs) inhibitor NPC-15437, altered behaviour and spinal PKMζ/PKCs expression were observed.A long-lasting hypersensitivity induced by Car-priming was identified and precipitated by subsequent plantar incision in this 'two-hit' paradigm. Post-treatment with ZIP, but not Scr-ZIP and NPC-15437, after the resolution of Car-priming selectively relieved hypersensitivity. In contrast, pre-priming NPC-15437 treatment only prevented Car-induced initial transient hyperalgesia. Immunoassays showed a significant decrease in spinal PKMζ expression after plantar incision with post-priming ZIP treatment as compared with Scr-ZIP and NPC-15437, but no notable differences in PKCs expression were observed.Spinal PKCs solely contribute to the initial induction of persistent pain, whereas PKMζ plays an essential role in spinal plasticity storage. PKMζ is responsible for the maintenance of peripheral inflammation-primed PPSP. Therefore, spinal PKMζ may be a therapeutic target to prevent surgery-induced chronic pain in patients with preoperative pain.

Poly(β-cyclodextrin) (PCD) resins were prepared by a crosslinking reaction of β-cyclodextrin with different amounts of epichlorhydrin. Some hydroxyl groups of these polymers were functionalized with alkyl quaternary ammonium groups. The polymers were tested for their ability to bind several bile salts (including the sodium salts of cholic acid, glycocholic acid, and chenodeoxycholic acid), individually and competitively, from phosphate buffer solutions. In all cases, the aminated PCD resin had a higher binding capacity for bile salts. The binding of chenodeoxycholate by the resins was much more effective than that of cholate and its conjugate, which indicates the importance of the host cavity size relative to that of the guest molecules. The degree of hydrophobicity of bile acids also plays a role in their binding by PCD resins. The variable temperature studies indicate that the electrostatic interactions become weaker at higher temperatures while the hydrophobic interactions are not as much affected.

This study was designed to test the hypothesis that a medium-term simulated microgravity by tail-suspension (SUS) induces hypertrophic and atrophic changes in the common carotid artery and abdominal aorta with their innermost smooth muscle (SM) layers being most profoundly affected. The second purpose was to elucidate whether vascular local renin-angiotensin system (L-RAS) plays an important role in the differential remodeling of the two kinds of large arteries by examining the gene and protein expression of angiotensinogen (AO) and angiotensin II receptor type 1 (AT1R) and their localization in the vessel wall. The results showed that SUS induced an increase in the media thickness of the common carotid artery due to hypertrophy of the four SM layers and a decrease in the total cross-sectional area of the nine SM layers of the abdominal aorta without significant change in its media thickness. Irrespective of the nature of remodeling, the most prominent changes were in the innermost layers. Immunohistochemistry, in situ hybridization, Western blot, and real time quantitative PCR analysis revealed that SUS induced an up- and down-regulation in AO and AT1R expression in the common carotid artery and abdominal aorta, respectively. In conclusion, our findings have demonstrated some special features in the structural adaptation of large elastic arteries due to a medium-term simulated microgravity.

Topographical and geological conditions variations cause serious static and imaging problems to the seismic data processing of the contract area, Block B, West Rub’ Al-Khali Basin. Although fairly good result was obtained with continuously effort for Near Surface Model (NSM) updating during the 2D and 3D seismic data processing, limited static correction accuracy was showed and possible static problems were also observed during later on data processing and interpretation. Bunches of near surface modeling methodologies can be adopted for near surface modeling and each one has its advantages and limitations in terms of adaptability and accuracy. Sorts of geosciences data are also available in the working area, Block B. By dedicated research and analysis all possible methodologies and geosciences data, a new term Near Surface Facies (NSF) was introduced and a new approach called Global Constrained Near Surface Modeling (GCNSM) was developed for the NSM building based on the NSF analysis. Comparing with the old NSM, the new model showed better accuracy and more reliable result.

Operations are typically associated with sleep and other circadian rhythm disturbances. The present study was set up to evaluate the influence of spinal and general anaesthesia associated with knee surgery on the circadian rhythm of melatonin, which has sleep inducing properties. Previously this context has been studied only in some invasive operations and it might be that general anaesthesia induces more disturbances on circadian rhythm of melatonin than operations done with patients awake.The circadian secretion pattern of melatonin was monitored during the pre- and postoperative evenings, nights and mornings to clarify possible anaesthesia/surgery-induced changes in the nocturnal secretion of melatonin and in the phase of the melatonin rhythm. The study included 20 patients scheduled for minor orthopaedic operations. The patients were randomised to receive either spinal or general anaesthesia. Melatonin was measured from evening and morning saliva samples radioimmunologically. The nocturnal urine before and after surgery was radioimmunologically examined for 6-hydroxymelatonin sulphate.Melatonin secretion evaluated from the saliva samples was significantly diminished during the first postoperative evening as compared with that during the preoperative evening (P0.001). There was also a significant decline of 26% (P0.05) in postoperative 6-hydroxymelatonin sulphate excretion. There was no significant difference in melatonin secretion between the spinal and general anaesthesia groups.Our findings suggest that anaesthesia in conjunction with surgery acutely disturbed the normal circadian rhythm of melatonin by delaying the onset of nocturnal melatonin secretion.

Angiopoietin-1 (Ang1) has been shown to act as an angiogenic promoter in embryonic angiogenesis by promoting vascular branching, pericyte recruitment and endothelial survival. We have investigated the role of Ang1 in tumour neovascularization under clinical conditions and in animal models. The expression of Ang1 in clinical breast cancer specimens was analysed by using laser-capture microdissection and reverse transcriptase-linked polymerase chain reaction (RT-PCR) on RNA isolated from the samples. Despite the expression of Ang1 in many human breast cancer cell lines, the gene was expressed in only three of 21 breast cancer clinical specimens, even though its receptor, Tie2, is abundant in the vasculature of all of these tumours. Ang1 was then overexpressed in a human breast cancer cell line (MCF-7) on its own and in conjunction with FGF1, an angiogenic factor shown to be able to increase the tumorigenicity of MCF-7 cells. High concentrations of Ang1 were produced in the conditioned media of the transfected cells (range 156–820 ng ml–1). However, in contrast to its physiological role as promoter of angiogenesis, overexpression of Ang1 did not enhance tumour growth, but instead caused up to a 3-fold retardation of tumour growth (P = 0.003). © 2000 Cancer Research Campaign

The effects of 60Co gamma rays on calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and neuropeptide Y (NPY)-immunoreactive nerve fibers were examined in mesenteric blood vessels of rats. Using a free-floating immunostaining streptavidin-biotin peroxidase complex method combined with a nickel-enhancement technique, we found that the distribution pattern of these two peptidergic nerve fibers in superior mesenteric arteries and superior mesenteric veins did not change, while the densities of CGRP-immunoreactive nerve fibers and NPY-immunoreactive nerve fibers in superior mesenteric arteries and veins varied with the time after irradiation. The results suggested that CGRP and NPY may be important in the development and elimination of radiation-induced injury.

The effect of microinjection of corticotropin releasing hormone (CRH) into the central nucleus of amygdala (CeA) on blood pressure was examined in Wistar rats anaesthetized with pentobarbital sodium. The results were as follows: (1) Microinjection of CRH 100, 500 ng into the CeA produced a dose-dependent increase in blood pressure. The response occurred 5 min after injection and lasted for at least 1 h. (2) The response could be blocked by injection of CRH receptor antagonist alpha-helical CRH9-41 into CeA. (3) Lateral ventricle injection of naloxone could also attenuate the pressor response of CRH. (4) After injection of alpha-helical CRH9-41 into nucleus of solitary tract, the pressor response of CRH was diminished. The results suggest that the pressor effect induced by microinjection of CRH into the CeA might be partly mediated through opioid system via a descending pathway from CeA to NTS.

The on-line prompt gamma neutron activation analysis (PGNAA) system is used to measure a series of prepared experimental samples to obtain the prompt characteristic γ-rays of the elements Ca, Fe, Si, Al, Mg and S in the examples of SiO2, Al2O3, MgO, CaO (powder), CaO (block), CaCO3, Fe2O3, Fe3O4 and S. The γ-ray energies are: Si, 3.54 or 4.93 MeV; Ca, 4.42 or 6.42 MeV; S, 5.42 MeV; Fe, 5.92 or 7.63 MeV. Meanwhile, the prompt γ-rays of the main elements are measured in an experiment in which SiO2, Al2O3, MgO, CaO (powder), CaO (block), CaCO3, Fe2O3, Fe3O4 and S are blended to constitute a cement sample. The prompt γ-rays of S will contribute more with increase in S mass, as known from the experiment concerning the prompt γ-ray spectrum for different masses of S.

The effects of histamine on action potentials (APs) and on early afterdepolarizations (EADs) were studied with standard microelectrode and computer in guinea pig papillary muscles. Experiments offer evidence that histamine (6.0 mumol/l) results in a significant diminution in action potential duration at 50% repolarization (APD50) and 90% repolarization (APD90) (data taken from no abnormal automaticity and oscillatory potential cells, 10 out of 19 preparations). Particularly, histamine can induce EADs (about 58%), oscillatory potentials and triggered activity. EADs frequently occur at low stimulation rates of 0.2-1.0 Hz and with prolongation of the action potential duration. EAD-induced triggered activity may exhibit fast spontaneous APs. Cimetidine (10 mumol/l, n = 6), an H2-receptor antagonist, antagonizes the effects of histamine. In the presence of cimetidine no EADs are observed by perfusing histamine. An H1-receptor antagonist, chlorpheniramine (10 mumol/l, n = 9), has no effect on the alterations of APs induced by histamine in ventricular myocardium. The results suggest that the mechanisms of histamine-induced shortness of AP duration, EADs and triggered activity may be related to the H2 receptor-mediated enhancement of the slow inward calcium current.

Through determining the rate and speed of extracting and the contents of effective constituents, an experimental comparison was made of the new TCM-decoction with the traditional decoction. The result showed that the new one was better than the traditional one.

To investigate the effects of the central alpha-receptors on the carotid sinus reflex, phenoxybenzamine (PBZ, 2 micrograms/20 microliters) or yohimbine (Y, 2 micrograms/20 microliters) was injected into the lateral cerebral ventricle (LCV) and intracarotid sinus pressure (ISP)-mean arterial pressure (MAP) relationship curve was constructed and fitted by a Logistic function. The results were as follows: (1) After injection of PBZ into LCV, the ISP-MAP relationship curve shifted upward at a higher ISP level with decreased peak slope and MAP range while the ISP-slope curve shifted downward. (2) After injection of Y into LCV, the ISP-MAP relationship curve shifted upward at a higher ISP level with decreased peak slope and MAP range and increased threshold. The ISP-slope curve shifted downward significantly at 10.64, 15.96 kPa. The peak slope and MAP range of Y group decreased significantly as compared with that of the PBZ group. These results indicated that after blockage of central alpha-receptors the sensitivity of the sinus reflex decreased significantly, and the blockage action of alpha 2-receptor is greater than that of alpha 1-receptor.

In order to study the effect of central noradrenergic system on the regulation of mean arterial pressure (MAP) by intracarotid sinus pressure (ISP), ISP-MAP relationship curves were constructed using isolated carotid sinus under the condition of injection of 6-OHDA (200 micrograms) into lateral ventricle or cauterization of locus coeruleus (LC) and compared with that obtained under the condition of control. All ISP-MAP curves can be fitted by a logistic functional plot whose characteristics can be defined by two parameters, i.e. coordinates of the inversion point of the slope of the curve and the MAP range related to the rate of change of slope factor with which carotid sinus pressure exerts its control. The results were as follows: After injection of 6-OHDA, the rate of change of the slope of the ISP-MAP curve is considerably slower than that of the normal control, thus showing a much compressed range of MAP by ISP, while the abscissa (i.e. position of ISP) of the slope inversion point of the curve shows practically no change. In comparison with the control, cauterization of LC causes a similar but less striking change of ISP-MAP curve than that of 6-OHDA injection experiment. All such differences were illustrated in Fig. 3. The above results indicated that the central noradrenergic system facilitates carotid sinus reflex and LC plays a dominant role.