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Objective To investigate the effects of plateau hypoxia on the growth and tumor microenvironment of colorectal carcinoma in vivo.Methods‍ ‍A total of 16 male BALB/C mice （6 weeks old， weight 18-20 g） were randomly divided into plateau hypoxic group and plain normoxic group， with 8 mice in each group， while 14 male C57BL/6 mice were grouped in same way， with 7 mice in each group. The mice in the plateau hypoxic group were housed in a low-pressure oxygen （10%） chamber to simulate an altitude of approximately 5 600 m， while the mice of the other group was maintained in SPF-grade normal atmospheric conditions （21% oxygen， at an altitude of about 300 m）. Colorectal tumor MC38 cells and colon adenocarcinoma CT26 cells were subcutaneously implanted into C57BL/6 mice and BALB/C mice， respectively to construct subcutaneous tumor-bearing mouse models. Then the tumor size and weight were measured in 4 groups of mice. After the tumor tissues， spleen and blood samples were collected in the C57BL/6 mice. Flow cytometry was used to determine the percentages of macrophages， T lymphocytes， IFN-γ+ T lymphocytes， and myeloid-derived suppressor cells （MDSC）. The differences in these immune cells were compared between the cells from the plateau hypoxic group and those from the plain normoxic group. Results‍ ‍The weight of subcutaneous tumor mass was significantly inhibited in both C57BL/6 and BALB/C mice from the plateau hypoxic group than those from the 2 plain normoxic groups （0.17 vs 0.09 g， 1.38 vs 0.51 g， P0.05）. Additionally， the percentage of CD4+ T cells was significantly reduced （48.70% vs 41.93%， P

Objective Constructing a depression risk prediction model for elderly people with chronic diseases living alone, to provide a scientific basis for early prevention and control of depression in the elderly.Methods Empirical analysis was conducted using the fifth round of China Health and Retirement Longitudinal Study (CHARLS) survey project. The Center for Epidemiological Studies Depression Scale (CSE-D) was used to evaluate the depressive mood of elderly people living alone with chronic diseases. Non-parametric tests and chi-square tests were used for univariate analysis, Lasso regression was used to screen potential predictive factors, and multiple Logistic regression was used to analyze the influencing factors of depression in elderly people living alone with chronic diseases. A depression risk prediction nomogram model for elderly people living alone with chronic diseases was constructed. The model was divided into a prediction set and a validation set by 7 ∶ 3 random splitting method. Receiver operating charcteristic(ROC) curve and area under cuve(AUC), H-L goodness of fit test, calibration curve graph, clinical decision curve, and clinical impact curve was used to evaluate the model.Results 522 elderly people with chronic diseases living alone were included in the study. The mean age was (69.54±6.56) years old. The prevalence of depression among elderly people with chronic diseases living alone in China was 50.38%. Self-rated health, life satisfaction, pain and discomfort, and internet use were the main influencing factors for depression in elderly people with chronic diseases living alone (P0.05). The goodness of fit is better for both groups, and the result of desicion curve and clinical impact curve showed that the risk prediction model has better calibration and net benefit.Conclusion The prevalence of depression is higher among elderly people with chronic diseases living alone, and descendent self-assessed health, decreased life satisfaction, pain, and lack of internet usage significantly increase the risk of depression among elderly people with chronic diseases living alone. This provides a basis for medical staff to conduct early and dynamic screening of depression among elderly people with chronic diseases living alone.

ObjectiveTo provide a reference for visual assessment in complex ocular trauma by studying the characteristics of visual evoked potential （VEP） in patients with different degrees of ocular trauma.MethodsBased on the severity of ocular trauma, 231 patients with monocular visual impairment were selected and divided into groups A （no open surgery, relatively mild injury）, B （open surgery, which may have a certain impact on the visual acuity after recovery） and C （multiple open surgeries, multiple intraocular surgeries or further treatment after surgery, and serious impact on the visual acuity after recovery）. At the same time, according to the best corrected visual acuity （BCVA） after recovery, they were divided into four grades: Ⅰ, visual

Abstract [Objective] PEX (pollen extensin-like) gene is involved in controlling pollen development, which plays a crucial role in embryogenesis of pollen microspores and haploid culture. In this study, the authors isolated the CaPEX3 gene from pepper and analyzed its protein structure and expression pattern, which provides a theoretical foundation for further investigation into the function of the CaPEX3 gene. [Methods] CaPEX3 was cloned by PCR, and its protein structure, evolution, and gene regulation were analyzed by bioinformatics. Expression of CaPEX3 in different tissues and pollen of different varieties were detected by qRT-PCR. Pollen viability of different pepper varieties was detected by staining method and their relationship with CaPEX3 expression was analyzed. [Results] The coding region of CaPEX3 was 1 581 bp in length, encoding 526 amino acids, which belongs to the LRX (leucine-rich repeats/extensins) gene family. The protein had phosphorylation and glycosylation sites, signaling peptides, and transmembrane domains. The expression level of CaPEX3 was the highest in flowers, and the gene expression was higher in tissues with high pollen vitality. The regulatory network predicted that CaPEX3 might be involved in the regulation of pollen development through the action of genes related to flower development. [Conclusion] CaPEX3 was specifically expressed in flowers, and a correlation was observed between its expression level and the activity of pollen grains. The analysis of gene expression and the construction of related vectors provided a theoretical foundation and technical support for the subsequent investigation of gene function and mechanism of CaPEX3

Abstract Aiming at the oscillation caused by the hydraulic cylinder of independent metering system (IMS) running between multiple working quadrants, a novel multiple quadrants switching displacement tracking control strategy is proposed in this paper. In this control strategy, both the cylinder chamber pressure under resistance and overrunning conditions are controlled by the inlet valve, and the outlet valve is utilized to control the displacement of cylinder. This method allows the hydraulic cylinder to always run in energy-efficient. When the working quadrant changes, the valve’s working modes are unchanged, which avoids oscillation caused by changes of valves working modes in principle. And a reduced-order active disturbance rejective displacement controller and an active disturbance rejective pressure controller are designed to get high-precision tracking of the displacement and pressure. Numerous experimental results show that the proposed method is reasonable and effective, hydraulic cylinder operates smoothly between multiple quadrants, the pressure and displacement controllers have good anti-interference ability and robustness, and the energy consumption can be reduced by more than 23% compared to the traditional constant pressure valve control system.

IntroductionOptimizing school physical education (PE) schedules is crucial for enhancing public health outcomes, particularly among school-aged children.MethodsTherefore, in this study, a weighted fitness function is developed to evaluate health fitness scores. This function integrates multiple health metrics such as BMI reduction, fitness improvement, calories burned, and heart rate reduction. Six optimization algorithms such as Genetic Algorithm (GA), Particle Swarm Optimization (PSO), Ant Colony Optimization (ACO), Simulated Annealing (SA), Differential Evolution (DE), and Artificial Bee Colony (ABC) optimization algorithms are utilized to optimize PE schedules based on the designed weighted fitness function. Using a dataset of 1,360 student entries, the study incorporates health metrics such as BMI reduction, fitness score improvement, caloric expenditure, and heart rate reduction into a weighted fitness function for optimization.ResultsThe results show that ACO achieved the highest allocation of PE time (9.91 h/week), the most significant caloric expenditure (370 kcal/session), and the greatest reduction in heart rate (8.5 bpm). GA excelled in the reduction of BMI, achieving a decrease of 10.63 units.DiscussionThese analyses reveal the transformative potential of optimized PE schedules in reducing the burden of lifestyle-related diseases, promoting equitable health outcomes, and supporting cognitive and mental well-being. Finally, recommendations are provided for policymakers and stakeholders to implement data-driven PE programs that maximize long-term public health benefits.

Neurological disorders pose significant global public health challenges, with a rising prevalence and complex pathophysiological mechanisms that impose substantial social and economic burdens. Traditional Chinese Medicine (TCM), with its holistic approach and multi-target effects, has gained increasing attention in the treatment of neurological diseases. This review explores bioactive compounds derived from Chinese herbal plants, focusing on their mechanisms of action, underlying pathways, and potential applications in functional food development. The review highlights the neuroprotective properties of flavonoids, alkaloids, polysaccharides, and polyphenols found in key TCM herbs such as Scutellaria baicalensis, Salvia miltiorrhiza, Ligusticum chuanxiong, and Gastrodia elata. These compounds have demonstrated significant anti-inflammatory, antioxidant, and neurogenic effects, making them promising candidates for the prevention and treatment of neurological conditions, including Alzheimer’s disease (AD), Parkinson’s disease (PD), and depression. Furthermore, the synergistic effects of TCM formulations targeting multiple signaling pathways offer advantages over single-target therapies, especially in combating neurodegenerative diseases. The review also discusses the challenges and future directions for integrating these bioactive compounds into functional foods and dietary supplements, aiming to improve neurological health and enhance clinical outcomes. Ultimately, this work aims to provide valuable insights into the potential of TCM-based interventions for promoting neurological well-being and addressing the global burden of neurological disorders.

Mucinous carcinoma is a rare clinical disease. Although well described in the literature, a mucinous carcinoma diagnosis is often difficult due to its atypical clinical presentation. We report a female patient with a right inguinal mass and ileocecal myxo carcinoma who was misdiagnosed as having a right incarcerated inguinal hernia invading the peritoneum incarcerated inguinal hernia and ileocecal myxo carcinoma. Intraoperative exploration of the mucous material occupying the patient’s right lower abdominal cavity and exclusion of right inguinal incarcerated hernia revealed the misdiagnosis. The first clinical manifestations of ileocecal mucinous carcinoma are often not readily apparent and may be misdiagnosed as an incarcerated inguinal hernia. When a color ultrasonography suggests an incarcerated inguinal hernia, an abdominal CT should be considered, and an enhanced CT should be performed as needed to observe the abdominal cavity. Ileocecal mucinous carcinoma should also be distinguished from other diseases with similar clinical manifestations. The patient had received conservative treatment for acute appendicitis, and it is recommended to conduct a B-ultrasound, CT, and other reviews after surgery. Clinicians should be aware of missed surgical opportunities following appendicitis caused by mucinous adenoma.

This article presents an in-depth analysis of global research trends in Geosciences from 2014 to 2023. By integrating bibliometric analysis with expert insights from the Deeptime Digital Earth (DDE) initiative, this article identifies key emerging themes shaping the landscape of Earth Sciences①.

Purpose – Temperature is an important load for a ballastless track. However, little research has been conducted on the dynamic responses when a train travels on a ballastless track under the temperature gradient. The dynamic responses under different temperature gradients of the slab are theoretically investigated in this work. Design/methodology/approach – Considering the moving train, the temperature gradient of the slab, and the gravity of the slab track, a dynamic model for a high-speed train that runs along the CRTS III slab track on subgrade is developed by a nonlinear coupled way in Abaqus. Findings – The results are as follows: (1) The upward transmission of the periodic deformation of the slab causes periodic track irregularity. (2) Because of the geometric constraint of limiting structures, the maximum bending stresses of the slab occur near the end of the slab under positive temperature gradients, but in the middle of the slab under negative temperature gradients. (3) The periodic deformation of the slab can induce periodic changes in the interlayer stiffness and contact status, leading to a large vibration of the slab. Because of the vibration-reduction capacity of the fastener and the larger mass of the concrete base, the accelerations of both the slab and concrete base are far less than the acceleration of the rail. Originality/value – This study reveals the influence mechanism of temperature gradient-induced periodic deformation in the dynamic responses of the train-track system, and it also provides a guide for the safe service of CRTS III slab track.

Haematococcus pluvialis residue powder, which has been extracted with astaxanthin, was used as the raw material to prepare Haematococcus pluvialis protein by alkaline extraction and acid precipitation method. The protein structure of Haematococcus pluvialis was characterized, and the effects of pH and ion strength on the functional characteristics of Haematococcus pluvialis protein were studied. The results showed that the isoelectric point of Haematococcus pluvialis protein was 4.2. The molecular weight of the subunit was around 14.4, 18.4 and 38 kDa, respectively and the content of α-helix, β-fold, β-corner and disordered structure in the secondary structure was 14.86%, 18.47%, 40.3% and 26.37%, respectively. It contained 7 kinds of essential amino acids required by the human body, accounting for 36.38% of the total amino acid content. Microscopic observation showed that the protein of Haematococcus pluvialis was rod-shaped and sheet-like, with a rough and porous surface. With the increase of pH, the solubility, water absorption, emulsion capability, foam stability and foam stability of the protein of Haematococcus pluvialis increased first and then decreased, while the emulsification stability showed the opposite trend. With the increase of NaCl concentration, the foam capability, foam stability, emulsion capability and emulsification stability of the protein of Haematococcus pluvialis increased first and then decreased, while the solubility showed a trend of first decreasing, then increasing, and then decreasing again.

Graphene-based metamaterial sensors are of significant research value for detecting food preservatives at low concentrations due to their extremely high sensitivity levels. In this work, we proposed and experimentally demonstrated an anapole resonance-based graphene metasurface (An-graphene-Ms) sensor with its conductivity altered by electrostatic doping effects for detecting and differentiating between two preservatives, sodium benzoate and potassium sorbate, in the terahertz region. Sodium benzoate, owing to its benzene ring structure, established π–π stacking interactions between the π-electrons in the benzene ring and those in graphene, amplifying the sensing effect. The amplitude changes and phase differences of the An-graphene-Ms sensor for the sodium benzoate detection were greater than those for potassium sorbate at the same concentration. Additionally, to reveal the dependence of the resonance frequency on the time delay, the measured signals were investigated using the continuous wavelet transform (CWT), and the time-frequency combination of the metasurface sensor was performed. The 2D wavelet coefficient intensity cards are effectively constructed through CWT, which also presents a more accurate approach for distinguishing and determining the concentrations of the two preservatives.

Abstract Aiming at the reasonable width of the narrow coal pillar of a fully mechanized caving face and the safety support of roadway, taking the coal pillar in the section between 110503 and 110505 face of the Yushuling Coal mine as the research background, a model of the hard basic roof fracture structure of fully mechanized caving face is established through theoretical analysis, and the roadway with narrow coal pillar is analyzed mechanically. Combined with the geological conditions of the working face, it is concluded that the low‐stress area is less than 3.29 m. When the internal stress field of the low‐stress environment is considered in the roadway layout, the influence of mining and the essential roof hardness should be considered. The reasonable size of the narrow coal pillar is 3 ~ 6 m, thinking that the load borne by the coal pillar is less than the ultimate strength of the coal pillar. The limit equilibrium theory calculates that the reasonable width of a coal pillar is at least 4 m. The stress and displacement of coal pillars with different widths of 3, 4, 5 and 6 m are analyzed by numerical simulation, and the 4 m narrow coal pillars are simulated and verified. Field industrial tests show that coal pillar and roadway surrounding rock deformation are small under asymmetric surrounding rock control. The research results have been successfully applied to engineering practice and can provide a reference for the research method of narrow coal pillar width under a hard basic roof.

Objective: To investigate the mediating effect of decreased Intrinsic capacity(IC) between symptoms and ability to perform activities of daily living(ADL) in elderly patients with Heart failure(HF). Methods: Using a convenience sampling method, 237 elderly patients with HF in the cardiology department of a Grade A Tertiary Hospital in Suzhou, China were selected from January 2022 to November 2022, and the General Information Questionnaire, Symptom Status Questionnaire-Heart Failure (SSQ-HF), Barthel Index Scale (BI), and IC Assessment Tool were applied to investigate the mediating effect of IC between HF symptoms and ADL using structural equation modeling. Results: HF symptoms and decreased IC were associated with patients' ability to perform ADL (P=0.005, P

Objective To analyze their molecular characteristics of microsatellite instability (MSI) by sequencing colorectal cancer patients in Sichuan region. Methods Next generation sequencing (NGS) was used to test 14 239 colon cancer patients from 2017 to 2022 in the Third People's Hospital of Chengdu for single nucleotide variation (SNV), insertion-deletion (InDel), copy number variation (CNV), fusion, MSI, tumor mutational burden (TMB), and Epstein-Barr virus (EBV). The clinical and molecular characteristics of highly microsatellite unstable (MSI-H) patients (MSI-H group, n=1 018) and microsatellite stable (MSS) patients (MSS group, n=13 221) were compared. Results The incidence rate of MSI in colorectal cancer was 7.15%, and detection rate of MSI varied in terms of age at diagnosis, tumour location and sample type. TMB was significantly higher in patients with MSI-H than in patients with microsatellite stable (MSS) (92.8 mutations/Mb vs 12.7 mutations/Mb, P＜0.05). EBV positivity in patients with colorectal cancer was 0.43%, but no patient with positive EBV was found in MSI-H. ERBB2, PIK3CA and BRAF all had significantly higher mutation frequencies in the MSI-H group, however, the positive rates of APC (51.26% vs 70.76%, χ2=168.823, P

In the design of the ecological seawall project for storm surge prevention on the north coast of Lingshan Island in the Greater Bay Area, to solve the contradiction between urban flood tide prevention and the hydrophilicity of the coastal landscape ecological seawall, the design is transformed from the traditional single tidal wave prevention seawall to the diversified coastal landscape ecological seawall by the concept of innovative technology. This paper innovatively proposes the following two technologies: the multi-stage wave dissipation platform technology and the upstream drainage system technology of coastal landscape belts to solve the problem of wave dissipation and drainage. In practice, the paper proposes the concepts of "horizontal for vertical" and "external drainage of external water and internal drainage of internal water" to break the traditional "blocking" and "resisting" of seawalls to the waves. It adopts the horizontal multi-stage wave dissipation platform technology to eliminate the waves and sets up a multi-stage drainage system to realize the self-exhaustion of the waves. The flexible articulation of "pass" and "discharge" for the waves on the waterfront can effectively reduce the vertical dike top elevation to solve the problem of "dike surrounding the city" and realize the purpose of coastal landscape ecological seawall. The research results have been applied to the project and have been tested by several storm surges to prove that the expected results have been achieved. In terms of the resilient city, especially in the design of the ecological dike, Lingshan Island exchanges the width for the height, which can not only prevent floods but also meet the requirements of water, leisure, and landscape. It provides experience and reference for the Lingnan region of ecological construction, and even the same type of construction in China's coastal estuarine areas.

To explain the moisture adsorption properties and improve the package material of polydextrose product, the adsorption and desorption isotherms of five domestic samples with initial moisture content (IMC) of 1.01%~4.89% were determined in this study in the range of 20~35 ℃ and ERH10%~90% using a dynamic water sorption analyzer. The adsorption and desorption isotherms were sigmoidal-shaped curves, and there was a hysteresis between desorption isotherm and the adsorption isotherm when ERH was less than 80%. Because polydextrose is an amorphous phase powder, we assumed that the water adsorption process of polydextrose was an equilibrium that existed among the vapor phase, the liquid phase (polylayer adsorption) and the solid solution (monolayer adsorption), and the adsorptive and desorptive sigmoidal-shaped isotherms can be de-convolved into a monolayer (Mh) and a polylayer component (Md) in accordance with Hailwood- Horrobin (HH) model. The polylayer water content of adsorption and desorption showed a sigmoidal increase with increasing ERH. The monolayer water content of desorption remained constant with increasing ERH, while that of the adsorption increased slowly for samples a1 to a4 with IMC 4.87% to 2.35% and decreased almost linearly for sample a5 with IMC 1.01%. As the IMCs of the samples decreased, the molecular weight of the polymer at the sorption site was maximum for sample a1 and minimum for sample a5. Although the FTIR showed the smallest intensity of hydroxyl group (3 416 cm–1) and amorphous structural functional group (1 027 cm–1) for a5 sample, a5 sample had the largest moisture absorption rate. These results suggested that, for the lower moisture content of polydextrose samples processed by different processes, their adsorptive monolayer water content seemed to decrease almost linearly with an increase of ERH. However their adsorptive polylayer water content sigmoidally increased with an increase of ERH, and their moisture absorption rate and hygroscopic capacity increased.

For seeking the cause of the increase in dough water absorption rate after cold plasma treatment, this study adopted low-pressure, radiofrequency 120 W helium cold plasma (CP) to treat wheat flours from 0 s to 180 s. Results showed that water absorption rate, conductivity, values of water and sucrose solvent retention capacity (SRC), and paste breakdown value in wheat flours increased significantly with increasing CP treatment time. To further explore the change causes in these physiochemical indexes, we developed a seven- parameter polynomial , (M was equilibrium moisture (%), ERH was equilibrium relative humidity (decimal), t was temperature (℃), and A~G were parameters. This polynomial could fit moisture sorption isotherms (20-35 ℃ and ERH 10%-90%) of helium CP treated flours, and the goodness-of-fit indexes were superior to those of modified Chung-Pfost (commonly used) and four-parameter polynomial (without temperature term) equations. The seven-parameter polynomial equation clearly showed the hysteresis loops between moisture adsorption and desorption isotherms of flour samples, which shifted downward with increasing temperature. The hysteresis degree showed a parabolic shape curve with increasing ERH, and the vertex of the parabola was at the range of 40-50% ERH. CP treatment shifted the monolayer adsorption sites to multi-molecular adsorption sites ahead of schedule, from ERH 50% to 40%. Further analysis using Dent model showed that the helium CP treatment was able to reduce the mono-layer moisture content and spreading pressure in wheat flour, and increase the elasticity of a capillary wall. The ability of water molecules was increased to form hydrogen bonds with the chemical components, which promoted the uniform distribution and permeation of water molecules into flour particles. Infrared spectral scanning showed that the degree of short-range order at the surface of starch granules and the interaction between protein and starch tended to increase with increasing CP time. Helium cold plasma technology has potential application in improving flour processing quality.

Objective To explore the role and molecular mechanism of Lnc-BM in the occurrence and development of gastric cancer (GC). Methods GC tissues and paired adjacent normal tissues of 36 GC patients were collected, and the expression of Lnc-BM was detected by RT-qPCR. Colony formation and CCK-8 assays were used to investigate the proliferation of GC cells. The migration and invasion properties of GC cells were investigated via Transwell assay. RNA pull-down assay was applied to confirm the interaction between FASTK and Lnc-BM. Western blot assay was used to detect FASTK protein level in Lnc-BM overexpressing or knockdown cells. Mitochondrial respiratory capacity and the related proteins expression levels were detected by Seahorse and Western blot assays, respectively. Lnc-BM stably overexpressing GC cells were constructed and then injected subcutaneously into nude mice. The tumor growth was observed. Results Lnc-BM was highly expressed in GC tissues compared with their paired adjacent normal tissues. Lnc-BM overexpression significantly promoted GC cells proliferation migration and invasion, while Lnc-BM knockdown inhibited GC cells proliferation, migration and invasion (P < 0.05). RNA pull-down experiment demonstrated that Lnc-BM can directly bind to FASTK. Western blot results indicated that overexpression of Lnc-BM increased the protein levels of FASTK, while knockdown of Lnc-BM inhibited the expression of FASTK (P < 0.05). Compared to the control group, overexpression of Lnc-BM increased the levels of mitochondria associated proteins, such as MT-ND6 and TOM20 (P < 0.05). Seahorse results indicated that overexpression of Lnc-BM enhanced mitochondrial respiratory capacity (P < 0.05). Knocking down FASTK in Lnc-BM stably overexpressing cells can reverse the increase in mitochondrial respiratory capacity caused by Lnc-BM overexpression (P < 0.05). In vivo, the results of subcutaneously implanted tumor model in nude mouse showed that Lnc-BM overexpression promoted the tumor growth (P < 0.05). Conclusion Lnc-BM promotes GC progression by regulating mitochondrial respiratory function through the FASTK/MT-ND6 axis.

To understand the cause for the increasing water absorption rate of wheat shorts after cold plasma treatment, a dynamic moisture adsorption instrument was used to measure the moisture sorption isotherms of samples treated with 120 W low-pressure strong radio frequency helium or oxygen CP for different times. The result was that the Caurie equation can fit an isotherm, and its coefficient C0 gived the density of water adsorbed by the sample. For adsorbed or desorbed samples, as the helium or oxygen CP treatment time at the same power increased, there was no difference in the density of adsorbed water, and the coefficient M0 gived the adsorbed water content of the monomolecular layer of the sample. For adsorbed or desorbed samples, as the helium CP treatment time at the same power increased, the water content of the monomolecular layer increased significantly. For samples treated with oxygen plasma, the content of the adsorbed monomolecular layer water first decreased and then increased as the treatment time increased, while the desorbed single-molecule water content showed a decreasing trend with the increased time. In order to improve the fitting of the Caurie equation, M0 was corrected to A+Bt (t was the temperature, A and B were constants). Analysis of the coefficients of the modified Caurie equation showed that as the 120 W helium CP treatment time (0~180 s) increased, the monomolecular layer of water, the number of adsorbed water molecule layers, the bound water content, and the water adsorption surface area all showed a continuous increase trend. For oxygen CP, these indicators showed an increasing trend in the first 60 s of processing, and then showed a decreasing trend if the processing time was extended. The results showed that compared with oxygen-cold plasma, high-energy helium-cold plasma improved the hydrophilicity of wheat shorts proteins and polysaccharides. As the treatment time increased, the surface area and number of layers of water adsorbed by these macromolecules and the monomolecular layer, and the bound water content steadily continued to increase.

Accurate evaluation of the safety status of the working face can promote the improvement of mine safety management level and disaster prevention and resilience. Using CH4 concentration, CO2 concentration, CO concentration, O2 concentration, temperature, and wind speed as evaluation indicators, the safety status of the working face is evaluated and analyzed. To reasonably determine the weight of evaluation indicators and improve the accuracy of safety evaluation results, the fuzzy analytical hierarchy process(FAHP) is used to calculate the subjective weight of evaluation indicators, and the G-GRITIC method is used to calculate the objective weight of indicators. The combination weighting method based on improved game theory (IGT) combines subjective weight with objective weight to obtain the combination weight of evaluation indicators, solving the problem of inconsistent subjective and objective information in the decision-making process. Based on the data collected by the safety monitoring system of the 209 fully mechanized working face of Shaanxi Huangling No.2 Coal Mine Co., Ltd., experimental verification is conducted on the IGT based combination weighting method. The results show that this method effectively avoids the subjective judgment of linear weighting method and average weighting method, optimizes the deviation results of the game theory (GT) combination weighting method. It obtains more reasonable evaluation indicators, which can obtain more accurate evaluation results of the safety status of the coal mine working face.

There are subtle differences between single regions of the same subcategory in fine-grained images. At present, many fine-grained image classification networks often focus on a single region to determine the target category. However, in many cases, most discriminative features in fine-grained images are distributed in multiple local regions of the image, and it is not often enough for fine-grained image to rely solely on one region.To solve these problems, a new method is proposed. This method generates discriminative features through reinforcement learning and obtains complementary regions through complementary network. The reinforcement network and the complementary network learn through adversarial learning and improve the accuracy of fine-grained images classification.The method is tested on CUB200-2011,fine-grained Visual Classification of Aircraft, and Stanford dogs datasets and the results show adequate performance.

Objective To analyze the list management mode in nosocomial infection prevention in the prosthodontics clinic. Methods Based on the analysis of problems found in nosocomial infection monitoring, a checklist management was implemented in prevention of nosocomial infection in the dental prosthesis clinic from August 2021 to December 2022, including air quality in the consulting room, medical devices, dental impressions, and hand hygiene of medical staff. After three times of airborne bacterial culture in the consulting room, sampling and bacteriological testing of the surfaces of instruments, dental impressions, and hands of medical staff, the monitoring results were recorded in detail. The quality monitoring data of nosocomial infection control in were compared before and after implementation, and the implementation effect was analyzed. Results The bacterial colony count of air in the consulting room was decreased after implementation of list management(P＜0. 01). The bacterial colony count of instrument surface, impression surface and hands of medical staff after implementation of list management were lower than those before implementation of list management (P＜0. 01). The qualified rates of colony monitoring on the air, hands of medical staff and the surface of the medical sterilizing tray were significantly higher than those before implementation of list management(P＜0. 01). The incidence of nosocomial infection after implementation of list management was significantly lower than that before implementation of list management (P＜0. 01). Conclusion The implementation of nosocomial infection list management can effectively reduce the bacteriological detection rate of air dust, instrument surface, impression surface and medical staff's hand, and reduce the risk of nosocomial infection, which is worthy of clinical promotion. (目的 分析口腔修复门诊实施院内感染清单式管理的效果。方法 针对院内感染监测发现的问题, 于2021年8月—2022年12月在医院口腔修复门诊实施包括诊室空气质量、医疗器械、口腔印模、医护人员手卫生的清单式管理。经过3次诊室空气培养, 器械表面、印模表面及医护人员手部采样和细菌学检测, 详细记录监测结果, 对比实施前后口腔修复门诊院内感染控制质量监测数据的差异, 分析实施效果。结果 清单式管理实施后, 口腔科门诊空气菌落计数较清单式管理实施前下降(P＜0. 01); 医护人员手部、口腔印模表面、医疗器械表面菌落计数较清单式管理实施前下降(P＜0. 01); 口腔科门诊空气、医护人员手部、托盘消毒储存监测合格率较清单式管理实施前提升(P＜0. 01); 患者院内感染发生率较实施前下降(P＜0. 01)。结论 口腔修复门诊实施院内感染清单式管理可有效减少诊室空气粉尘、器械表面、印模表面、医护人员手部的细菌学检出率, 降低院内感染风险。)

Objective To explore the effect of systematic oral care interventions on dental plaque control and oral health care behavior in patients with periodontal disease. Methods From March 2022 to April 2023, 100 patients with periodontal disease were divided into the control group and observation group by drawing-lots method. The control group(n=50) received routine nursing interventions, and the observation group(n=50) received systematic oral nursing intervention. The effect of interventions on dental plaque control and oral health care behavior was evaluated and compared between two groups. Results After 3 months of nursing interventions, the gingival bleeding index, periodontal pocket probing depth and dental plaque index in the observation group were lower than those in the control group, with a significant difference (P＜0. 01). The qualified rate of oral health care behavior in observation group was higher than that in control group (P＜0. 01). The hospital revisit rate, correct answer fill rate of oral health care knowledge questionnaire, compliance rate of oral health care behavior in the observation group were higher than those in control group (P＜0. 01). Conclusion The implementation of systematic oral nursing interventions can improve the effect of plaque control and the qualified rate of oral health care behavior in patients with periodontal disease. (目的 研究系统口腔护理干预对牙周病患者牙菌斑控制效果及口腔保健行为的影响。方法 选取2022年3月—2023年4月医院收治100例牙周病患者为研究对象, 经抽签法分为对照组和观察组, 每组50例。对照组实施常规护理干预, 观察组实施系统口腔护理干预。评估两组牙菌斑控制效果和口腔保健行为。结果 护理3个月后, 观察组牙龈出血指数、牙周袋探诊深度、牙菌斑指数低于对照组, 差异有统计学意义(P＜0. 01)。观察组口腔保健行为达标率高于对照组(P＜0. 01)。观察组患者按时复诊率、口腔卫生健康知识回答正确率、口腔卫生习惯依从性均高于对照组(P＜0. 01)。结论 牙周病患者实施系统口腔护理干预, 可提高牙菌斑控制效果及口腔保健行为达标率, 值得临床借鉴。)

[Objective] To study the physiological response of sunflowers to broomrape parasitism and the related metabolic pathways, as well as understanding the molecular mechanism of sunflower resistance to broomrape parasitism. [Methods] The broomrape-resisant (s41) and -sensitive (s26) sunflowers were used as the testing materials. Transcriptome analysis of 4 samples under the condition of parasitic stress was carried out, and physiological verification analysis of key metabolites was carried out. [Results] (1) Under inoculation, the plant height and root number of susceptible materials were affected significantly, and some of them were even unearthed, while the resistant materials had no obvious changes, and only a few were parasitized. (2) The number of DEGs was 6 362, 6 609, 5 490 and 5 469 by screening the differential genes among the samples. GO analysis showed that the four comparison groups, namely s26-s41, s26CK-s41CK, s26-s26CK, and s41-s41CK, had the most differential genes in biological processes, followed by cellular components, and less in molecular functions. KEGG analysis showed that infection had a great influence on plant signal transduction, biosynthesis of secondary metabolites, lipid metabolism, amino acid metabolism, and sugar metabolism in sunflowers. (3) Physiological analyses found that the phenylalanine deaminase, cinnamyl alcohol dehydrogenase, glutathione, total phenols, and salicylic acid showed an upward trend, indicating that the stress tolerant system was activated under parasitism. However, the trend of the physiological indicators of the sensitive and tolerant materials were different. Also, the genes controlling the physiological responses were differentially expressed. [Conclusion] It was found that inoculation with broomrape significantly affected the plant height and root volume of the susceptible materials, while the resistant materials were not affected. The resistant materials were enhanced in root lignin synthesis, antioxidant enzyme activity, and the expression of key genes of phenylpropanoid metabolism, salicylic acid and signaling pathway, and hence increased the resistance to broomrape.

ABSTRACTUnintended effects of gene edit crops may pose safety issues. Omics is a useful tool for researchers to evaluate these unexpected effects. Transcriptome and proteomics analyses were performed for two gene editors, CRISPR-Cas9 and adenine base editor (ABE) gene edit rice, as well as corresponding wild-type plants (Nipponbare). Transcriptome revealed 520 and 566 rice differentially expressed genes (DEGs) in the Cas9/Nip and ABE/Nip comparisons, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most DEGs participated in metabolism of terpenoids and polyketones, plant–pathogen interactions, and plant signal transduction. It mainly belongs to environmental adaptation. Proteomics revealed 298 and 54 rice differentially expressed proteins (DEPs) in the Cas9/Nip and ABE/Nip comparisons, respectively. KEGG pathway enrichment analysis showed that most DEPs participated in the biosynthesis of secondary metabolite and metabolic pathways.According to integrated transcriptomes and proteomics analysis, the results showed that no newly generated genes were identified as new transcripts of these differentially expressed genes, and gene edit tools had little effect on rice transcription levels and no new proteins were generated in the gene-edited rice.

The surface point cloud data of coal mine roadways extracted using 3D laser scanning technology has a large amount of redundant data. The existing point cloud data simplification methods have the problem of insufficient detail preservation in the processing of large-scale point clouds. In order to solve the above problems, a surface point cloud data reduction method for coal mine roadways based on secondary feature extraction is proposed. Firstly, the method performs denoising preprocessing on the collected original roadway point cloud data. Secondly, the method establishes a K-d tree and uses principal component analysis to estimate the denoised point cloud data to fit the normal vector of the neighborhood plane. Thirdly, the point cloud is preliminarily divided into feature and non-feature regions using a smaller normal vector angle threshold, retaining the feature regions and randomly downsampling the non-feature regions. Fourthly, based on the larger normal vector angle threshold, the feature region point cloud is divided into feature points and non-feature points. And voxel random sampling is conducted on the non-feature points. Finally, the method merges the two point cloud simplification results with the feature points to obtain the final simplified data. The simulation results show that under million data level point clouds and high precision conditions, this method achieves better results in feature preservation and reconstruction precision compared to curvature simplification methods, random simplification methods, and grid reduction methods. The average standard deviation calculated after 3D reconstruction can be about 30% lower than other methods under the same reduction rate.

Quantitative studies of mangrove leaf functional traits will help us understand the adaptive evolutionary strategies of mangrove plants and the relationship between mangrove biodiversity and ecosystem functions. Because of the special environment of the intertidal zone where mangroves are located, it is very difficult to obtain the functional traits of mangrove canopies from the ground, and relevant studies are lacking. The maturity of Unmanned Aerial Vehicle (UAV) hyperspectral remote sensing technology provides a new means of conducting such research. This study considered mangroves on Qi'ao Island, Zhuhai, as the research object. Based on UAV hyperspectral data, two UAV hyperspectral data processing methods, which combined Partial Least Squares Regression with Normalized Difference Vegetation Index (PLSR+NDVI) and Partial Least Squares Regression with Continuous Wavelet Transform (PLSR+CWT), were used to estimate the 10 canopy leaf functional traits of mangroves on Qi'ao Island. The results showed that the PLSR + NDVI method was more suitable for the inversion of mangrove canopy-specific leaf weight (LMA), phosphorus content per unit mass (Pmass), and nitrogen content per unit area (Narea), whereas the PLSR + CWT method was more suitable for the estimation of the nitrogen/phosphorus ratio (N/P), chlorophyll content (Cab), and carotenoid content (Cxc). However, the results of the above two methods for retrieving the nitrogen content per unit mass (Nmass), potassium content per unit mass (Kmass), phosphorus content per unit area (Parea), and potassium content per unit area (Karea) were not ideal (R2

The rotor system of the centrifugal compressor unit comprises the rotor and the support structure. The change in the rotor balance state and the abnormal stiffness of the composite spring are common faults of the rotor system. However, the simultaneous occurrence of the above two will result in an abnormal vibration that is not easy to be distinguished. In order to identify and diagnose such faults accurately, a rotor vibration analysis model was established to analyze the change in the rotor unbalance state and the abnormal stiffness of composite spring at different rotating speeds. Meanwhile, the influence of such faults on vibration was studied. The results show that the rotor vibration response will produce a series of regular changes when the above two faults occur. The relevant theories were verified by practical application, providing theoretical and practical support for the identification and analysis of the fault. Generally,the research results could assist the field staff to find the abnormal state of rotor balance, the abnormal stiffness of the composite spring,and the complex fault at the simultaneous occurrence of the two. This is helpful to judge the fault location, find out the fault cause and formulate the targeted maintenance plan, so as to ensure the safe operation of centrifugal compressor units in long-distance natural gas pipelines.

To reveal the effects of the microstructure characteristics including fiber shape, void, fiber distribution pattern, and interphase on the transverse moisture diffusivities of unidirectional composites, the steady analysis method based on Fick’s law is adopted. The predicted numerical results are compared with the results from the analytical models to demonstrate the accuracy. From the simulation results, it is found that the increase in the oscillation amplification of non-circular fibers contributes to the orthotropy of diffusivity properties, which attributes to different barrier effects along different directions. The consideration of interphase relieves the barrier effects and the predicted diffusivity values are increased significantly. The effects of voids increase with the void volume fraction and are dependent on the voids’ location. If the fiber random distribution pattern is considered, it is found that the average values of predicted diffusivity decrease gradually with the increase in the number of oscillations.

Objective To summarize and analyze the clinical features and comorbidity of attention deficit and hyperactive disorder (ADHD) with self⁃limited epilepsy with centrotemporal spikes (SeLECTS) in children. Methods A total of 89 SeLECTS patients diagnosed in Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology from July 2020 to December 2022 were selected. The social demographic data, clinical manifestation, video electroencephalography (VEEG) results, treatment and prognosis of the children were collected. Swanson, Nolan and Pelham⁃Ⅳ Rating Scale (SNAP⁃Ⅳ) was used to assess the comorbidity of ADHD. Results 1) Clinical features: there were 49 males and 40 females in 89 cases of SeLECTS. The age of onset was 3-12 years old, with an average of (7.22 ± 2.04) years old. The main seizure forms were motor seizures of focal origin (36 cases, 40.45%) and focal progression to bilateral tonic ⁃ clonic seizures (46 cases, 51.69%), and 7 cases (7.87%) had both. Main manifestations were unilateral clonic or tonic⁃clonic seizures, drooling, strabismus or upward movement of eyes, abnormal oral movement, and throat vocalization. 2) EEG characteristics: background activity of VEEG of 56 patients was normal, and epileptiform discharge waveforms were mainly sharp (spike) waves, sharp (spike) slow waves complex, spike clusters, and electrical status epilepticus during sleep (ESES). Spike wave index (SWI) during slow wave sleep was 33.00 (19.25, 53.25). 3) Treatment and prognosis: 37 cases (41.57%) were seizure⁃free at least 6 months, and 23 cases (25.84%) were seizure⁃free at least 12 months. 4) Comorbidity: 44 patients (49.44%) had comorbid ADHD. Compared with the non⁃ADHD group, the ADHD group had a younger age of onset (t = 1.621, P = 0.002), a higher SWI (Z = ⁃ 2.134, P = 0.038) and a higher incidence of ESES (χ2 = 4.308, P = 0.038). Conclusions SeLECTS is a self⁃limited epilepsy syndrome in children with characteristic clinical and electroencephalographic features. For children with younger age, higher SWI and ESES, comorbid ADHD should be considered.

Objective To investigate the neural network mechanism of fear induced by cortical functional electrical stimulation with stereotactic electrodes. Methods and Results A total of 20 patients with intractable epilepsy who were diagnosed and underwent stereotactic electrode implantation in Sanbo Brain Hospital of Capital Medical University from January 2021 to June 2022 were selected. Among them, 7 patients (35%) received 75 reconstructed electrode contacts [9.66% (75/776)] to induce fear emotional response. They were mainly distributed in temporal lobe [45.33% (34/75)], insula lobe [32% (24/75)] and cingulate gyrus [13.33% (10/75)]. According to the "seed and discovery" calculation method, the occurrence probability of evoked fear response electrode contacts in 9 general brain networks (auditory, left executive control, right executive control, sensorimotor, visuospatial, dorsal default mode, advanced vision, posterior salience and primary vision) was the highest in the dorsal default mode network, accounting for 84% (63/75). The whole brain functional connection values of the fear⁃inducing electrode contacts were retrieved from the neurosynth database, and the differences between the in⁃brain and out⁃brain functional connection values were calculated. It was found that the matching degree between the fear⁃inducing electrode contacts and the dorsal default mode network was the highest, followed by the posterior prominence network. Conclusions Based on stereo⁃electroencephalography (EEG), the fear response induced by cortical functional electrical stimulation was mainly related to the dorsal default mode network. The hippocampus, insula and cingulate gyrus are important network nodes of fear response. It is suggested that there is a shared neural circuit between the fear response induced by electrical stimulation and the fear experience acquired through conditioning.

Deep brain stimulation (DBS), an important form of neuromodulation therapy, provides a new treatment option for drug⁃resistant epilepsy (DRE) patients through precisely selections the stimulation target. By implanting electrodes at specific targets and releasing electrical stimulation, DBS can regulate the excitability of the relevant nuclei and neural circuits in the process of seizure, so as to exert the effect of controlling seizure. At present, some clinical and laboratory evidence has been accumulated on the regulatory mechanism of DBS in the treatment of DRE, the individualized selection of stimulation targets for different types of seizures, the curative effect and adverse events. This article will elaborate the above three aspects, in an effort to promote the application of DBS in DRE.

Objective To analyze the seizure characteristics of epilepsy in the course of different types of autoimmune encephalitis (AE), and to screen the markers of early diagnosis value. Methods A total of 91 patients with AE with epileptic seizure admitted to Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology from August 2014 to January 2021 were collected. The clinical data included social demographic data, pathogenic characteristics, EEG and imaging characteristics, treatment and prognosis. Results 1) Anti⁃N⁃methyl⁃D⁃aspartate receptor (NMDAR) encephalitis (n=58), anti⁃γ⁃aminobutyric acid receptor type B (GABABR) antibody⁃associated encephalitis (n=12) and anti⁃leucine rich glioma inactivated protein 1 (LGI1) antibody⁃associated encephalitis (n=12) were common types of AE. 2) Rapid progressive dementia [79.12% (72/91)], sleep disorders [79.12% (72/91)] and mental disorders [75.82% (69/91)] were the main non⁃epileptic symptoms, but mood disorders or speech disorders were rare. 3) In the course of encephalitis, 76.92% (70/91) of patients had generalized tonic⁃clonic seizure (GTCS), which were the characteristic seizure type of anti⁃GABABR antibody⁃associated encephalitis (12/12). While faciobrachial dystonic seizure (FBDS) was the specific seizure type of anti⁃LGI1 antibody⁃associated encephalitis [4.40% (4/91)]. 4) The main manifestations of VEEG were background slow waves [37.88% (25/66)], slow waves combined with epileptic discharges [27.27% (18/66)] or epileptic discharges [18.18% (12/66)], but the increase of fast wave activity [1.52% (1/66)] was rare. There was also no abnormal brain wave [15.15% (10/66)]; δ brush was observed in only 2 patients with anti⁃NMDAR encephalitis. 5) MRI abnormalities [58.89% (53/90)] were mainly found in hippocampus amd temporal lobe, characterized by T2WI and FLAIR hyperintersity, mainly in patients with anti⁃LGI1 antibody⁃associated encephalitis (9/12). 6) Immunotherapy [97.80% (89/91)] and antiepileptic seizure medicine [94.51% (86/91)] were the preferred treatment options. The mean follow⁃up was 48.50 months, and the fatality rate was 14.29% (10/70), with the highest rate of anti⁃GABABR antibody⁃associated encephalitis (4/9). The recurrence rate of epilepsy was 10.17% (6/59), and the long⁃term recurrence rate of anti⁃DPPX antibody⁃associated encephalitis was relatively high (2/3). Conclusions Different types of AE with epileptic seizure have specific clinical characteristics, which is of great significance for early diagnosis and prognosis.

With the continuous exploration and development of shale gas in China, the complex structure area has become an important direction for further exploration. The Longmaxi shale samples collected from complex structure area in Northeast Chongqing were conducted scanning electron microscopy, mercury intrusion, low-pressure gas adsorption, isothermal adsorption. This study systematically characterized the pore structure and adsorption capacity of Longmaxi shale in different tectonic belts, analyzed the influencing factors of pore structure and adsorption capacity, and revealed the action mechanism of tectonic deformation on pore structure and adsorption capacity. The results showed that ① OM pores are relatively developed in the Longmaxi shales collected from thrust slip belt and thrust fold belt, but not in the Longmaxi shale from imbricate thrust belt, where mineral-related pores and fractures are mainly developed. ② Micropores (50 nm) are developed in the Longmaxi shale in the thrust slip belt, while mesopores and macropores are relatively developed in the thrust fold belt and imbricate thrust belt. ③ The “excess” adsorption capacity of Longmaxi shale rapidly increases with the increased pressure, and slowly decreases after reaching the maximum (6−9 MPa), while the absolute adsorption capacity monotonously increases with the increased pressure. ④ The pore structure is mainly controlled by TOC (Total Organic Carbon) and clay mineral content, and the adsorption capacity is closely related to TOC and micropores. In addition, tectonic deformation can also affect the adsorption capacity by modifying the pore structure. This study has a very important guiding significance for the evaluation and development of shale gas resources in complex tectonic areas.

Aiming at the problems of complex operating conditions, large load disturbance, strong coupling of pressure and position control subsystems, and time-varying parameters of independent metering electro-hydraulic servo system (IMEHSS), an active disturbance rejection decoupling (ADRC) control method with online compensation information is proposed in this paper. In this study, the internal coupling of subsystems is treated as the part of total disturbances, and decoupling control is realized by estimating and compensating the total disturbance through extended state observer. In position control subsystem, the cylinder dynamic changes caused by the inlet chamber flow rate of cylinder and the deviation of the nominal calculated flow rate from actual calculated flow rate are calculated in real time. And these calculated values are compensated to the controller as online information to improve the control accuracy and the disturbance rejection ability of position control. By calculating the cylinder velocity in real time to obtain the effect of cylinder speed on the change of return chamber pressure and compensating it into the controller to improve the return chamber pressure control accuracy. The online information compensation method presented in this paper reduces the amount of total disturbance and thus reduces the burden of extended state observer. At the same time, this method can realize the proportional relationship between the regulation parameters and control signals in the passive pressure control subsystem, which simplifies the tuning process of parameters. Finally, to verify the effectiveness and rationality of the method, simulations and experiments are carried out.

There are some shortcomings for Xinjiang Naan prepared by traditional process. In this paper, Naan was prepared by oven. The amino acid composition, flavor substances and infrared spectra of self- made electric oven Naan were investigated, and compared to the commercial Naan. The experimental results showed that there were 17 kinds of amino acids in all samples, and the total amino acid content of self-made Naan was higher than that of commercial naan (P

Objective To evaluate the application of personalized oral care in patients with oral and maxillofacial diseases. Methods Totally 200 patients with oral and maxillofacial diseases were randomly divided into two groups. Patients in the control group(n=100) were given routine oral care during the treatment, and those in the observation group(n=100) were given personalized oral care based on routine management. The therapeutic effect, incidence of complications, patient satisfaction with nursing service and compliance rate of the two groups were evaluated and compared. Results The therapeutic effect in the observation group was higher than that in the control group (98. 00% vs. 89. 00%, P＜0. 05 ). The satisfaction rate, incidence of complications and compliance rate of the observation group were better than those of the control group (P＜0. 05). Conclusion Personalized oral care could effectively improve the treatment effect, satisfaction and compliance rate of patients, facilitate the development of diagnosis and treatment, and then help to reduce the incidence of complications, promote the rehabilitation of patients and improve the quality of life. (目的 探讨个性化口腔护理在口腔颌面疾病中的应用效果。方法 将2019年1月—2021年1月收治的200例口腔颌面疾病患者随机分为观察组和对照组, 各100例。对照组给予常规护理, 观察组在对照组基础上开展个性化护理措施。观察组两组治疗效果、护理满意度、并发症发生情况以及治疗依从性。结果 观察组治疗总有效率98. 00%(98/100), 高于对照组的89. 00%(89/100), 差异有统计学意义(P＜0. 05)。观察组护理满意度91. 00%(91/100), 高于对照组的74. 00%(74/100), 差异有统计学意义(P＜0. 05)。观察组并发症发生情况优于对照组, 差异有统计学意义(P＜0. 05)。观察组依从率92. 00%(92/100), 高于对照组的83. 00%(83/100), 差异有统计学意义(P＜0. 05)。结论 口腔颌面疾病诊疗过程中采用个性化口腔护理干预, 有助于提高患者治疗依从性, 促进口腔功能恢复, 值得临床推广应用。)

Objective: This study was to explore the improvement of structural and emulsifying properties of rice protein by interacted with the dark blue pigment of Vaccinium bracteatum Thunb leaves. Methods: The dark blue pigment was prepared from VBTL, and the changes of structural and emulsifying properties of rice protein before and after dyeing were analyzed. Results: The formation of binary complexes between the dark blue pigment and the rice protein decreased the color values of L* and b*, and the hue of the pigment-protein complex changed to dark blue color with the increase of the pigment concentration. The water solubility of the pigment-protein complex increased, and the oil-holding capacity decreased significantly. The analysis circular dichroism spectroscopy showed the changes in the secondary structure of pigment-protein complex with a decreasing in the relative content of α-helices and β-folds and an increasing in the relative content of β-turns and random coil. At the concentration of pigment below 1.5%, foam stability increased, while foaming capacity, emulsifying capacity and emulsion stability decreased. When the concentration of pigment was higher than 1.5%, foam stability decreased, while foaming capacity, emulsifying capacity and emulsion stability increased. Conclusion: The dark blue pigment can improve the emulsifying properties of rice protein by changing its secondary structure.

[Objective] The particle size characteristics and sedimentary environment of dune sediments in the southwest margin of Qaidam Basin were analyzed in order to provide basic data to support sand control and safe operation of the Golmud-Korla Railway. [Methods] We used the particle-size analysis method to determine the particle-size characteristics and sedimentary environment of dunes in the piedmont of the Southwestern Qaidam Basin. [Results] The dunes in the Southwestern Qaidam sand area were mainly composed of fine sand, followed by medium sand. The mean particle-size was between -0.14 Φ and 3.49 Φ, with an average of 2.02 Φ. The sorting parameters varied from 0.32 to 1.59 (with average of 0.66). The skewness distribution was nearly symmetric. The kurtosis was dominated by moderate kurtosis. The natural frequency distribution curves were uni-modal and bi-modal. The cumulative frequency curves were one-stage, two-stage, and three-stage. Mean particle-size and sorting showed a linear correlation relationship, i.e., as mean grain-size became finer, sorting gradually became better. Mean particle-size and kurtosis were negatively correlated when the peak kurtosis value was less than 0.9. In contrast, when the peak kurtosis value was greater than 0.9, mean particle-size and kurtosis were positively correlated. [Conclusion] The northwestern part of the Southwestern Qaidam Basin is an aeolian environment dominated by aeolian sands. The southeastern part of the area is dominated by lacustrine and alluvial-fluvial environments, and the dune sands are affected by the interaction of wind and water.

Sharding is a technology that solves the problem of blockchain capacity expansion.However,sharding may make it ea-sier for malicious nodes to be concentrated in a single shard,thus hindering the safe operation of the entire system.This paper proposes a reputation-based sharding consensus protocol(RBSCP),which establishes a reputation mechanism to measure node behavior and encourage nodes to follow the protocol.The reputation level-based sharding method reduces the difference in the reputation level distribution in different shards,so as to prevent malicious nodes from concentrating on a single shard to do evil.A double-chain model combining verification chain and record chain is proposed.Through the differentiated storage of transactions,the storage capacity of the blockchain is expanded while the security of the blockchain is improved.By associating the vo-ting shares with the node reputation and differentiating the node commitments,a reputation-based fast Byzantine fault tolerance(RFBFT) algorithm is proposed,which enables honest nodes to reach consensus faster and reduces the impact of malicious nodes.Security analysis shows that RBSCP can guarantee the rationality of node distribution in shards and the security of consensus process,and prevent double spend attack and nothing at stake attack.Experimental results show that RBSCP can achieve low sharding latency,low consensus latency and high throughput under the premise of ensuring security.

237Np is a highly toxic nuclide, which can cause internal exposure hazards after inhalation. The analysis of 237Np based on urine samples is an important method for routine monitoring, as well as a key method for studying the dose level of 237Np in the human body of professional workers. The pretreatment process of 237Np in large-volume urine samples, pre-concentration by co-precipitation, and the effect of TEVA resin separation and purification and electrodeposition on the chemical recovery of 237Np were systematically studied and discussed. The research results show that the chemical recovery of the experimental method can reach 88.5%, and the detection limit of the method is 2.55×10-5 Bq/L. This analysis method meets the requirements of routine monitoring and is suitable for monitoring and evaluating 237Np in large-volume urine samples.

The evaluation of system performance plays an increasingly important role in the reliability analysis of cyber-physical systems. Factors of external instability affect the evaluation results in complex systems. Taking the running gear in high-speed trains as an example, its complex operating environment is the most critical factor affecting the performance evaluation design. In order to optimize the evaluation while improving accuracy, this paper develops a performance evaluation method based on slow feature analysis and a hidden Markov model (SFA-HMM). The utilization of SFA can screen out the slowest features as HMM inputs, based on which a new HMM is established for performance evaluation of running gear systems. In addition to directly classical performance evaluation for running gear systems of high-speed trains, the slow feature statistic is proposed to detect the difference in the system state through test data, and then eliminate the error evaluation of the HMM in the stable state. In addition, indicator planning and status classification of the data are performed through historical information and expert knowledge. Finally, a case study of the running gear system in high-speed trains is discussed. After comparison, the result shows that the proposed method can enhance evaluation performance.

In the diffraction limit storage ring, the size of the vacuum chamber was greatly reduced compared with the third-generation synchronous light source due to the high-gradient small-aperture magnet, and the impedance of ring is significantly increased. Therefore, it is necessary to accurately evaluate and optimize model of the impedance of the vacuum elements in the design stage. In the impedance budget, the resistance wall impedance accounts for a high proportion of the whole ring impedance, and a sharp resonance peak will appear when the frequency is close to 0, which has a great impact on the beam motion. In the RF system, the high-order mode of the cavity is an important source of the narrow-band impedance. Therefore, in the accelerator design stage, it is necessary to select the resistance wall of appropriate materials, accurately model of the RF cavity and the third harmonic cavity, calculate the impedance, and evaluate instability that may be caused. In this paper, the multi-layer resistance wall impedance of the medium energy diffraction limit storage ring was calculated and the optimized material of resistance wall was chosen for the diffraction limit storage ring. A vacuum chamber with distributed exhaust is required in the storage ring, and the thickness of copper coating of 1 μm film is the optimized material. For special vacuum chamber components, the internal structure is complex and it is impossible or difficult to implement NEG coating, or the centralized arrangement of air pump can meet the vacuum requirements, the stainless-steel internal coating with 5 μm copper film is considered. The impedance models of RF cavity and the third harmonic cavity were established and optimized by using numerical simulation program. The fluted beam tube can effectively transmit the high-order modes generated in the RF cavity and the third harmonic cavity to ensure the stability of the beam. The collective effects were caused by resistive wall impedance, the high order modes of RF cavity and the third harmonic cavity were analyzed and summarized. In the medium energy diffraction limit storage ring, the transverse impedance of the resistance wall will cause the transverse coupled multi beam instability, the impedance of the RF cavity and the third harmonic cavity will not cause the longitudinally coupled multi beam instability, and will not become a limiting factor for the improvement of the beam intensity in the subsequent light source upgraded. Meanwhile, effective control measures are put forward to solve the problems of beam instability. For transverse coupling multi beam instability, positive chromatic can be effectively suppressed.

Non-Line-of-Sight (NLOS) Underwater Wireless Optical Communication (UWOC) is mainly realized through the air-seawater interface reflection or the scattering of light by particles in seawater. It can effectively solve the link alignment issues of Line-of-Sight (LOS) underwater wireless optical communication. This paper summarizes the research progress of NLOS UWOC technology in the aspects of channel model, simulation and experiment. At present, scholars mainly explore the effects of water quality, wave, turbulence, modulation technology, receiving and sending terminal configuration on the performance of NLOS UWOC link. Few scholars have studied the effects of complex hydrodynamic and water quality factors, non-linearity of light source, non-linearity, sensitivity and noise of detector on the performance of NLOS UWOC and effective solutions. Therefore, it is necessary to carry out more in-depth channel model, simulation and experimental research in the future, so as to promote the development of NLOS UWOC technology.

Objective To summarize the clinical characteristics of anti⁃myelin oligodendrocyte glycoprotein⁃IgG associated disorders (MOGAD) complicated with antibody overlap syndrome. Methods and Results Ten patients with MOGAD complicated with antibody overlap syndrome treated in the First Affiliated Hospital of Zhengzhou University and the Fifth Affiliated Hospital of Zhengzhou University from April 2016 to April 2021 were included, including 7 patients with N⁃methyl⁃D aspartate receptor (NMDAR)⁃IgG positive, 2 patients with contactin⁃associated protein 2 (CASPR2)⁃IgG positive and one patient with glial fibrillary acidic protein (GFAP)⁃IgG. The ratio of male to female was 1∶2.33, and the average age of onset was 20.70 years old. Five cases had a history of precursor infection. Headache and fever were the most common initial symptoms. The average Expanded Disability Status Scale (EDSS) score at admission was 2.40. Intracranial pressure (ICP) increased in 5 cases, white blood cell count in cerebrospinal fluid (CSF) increased in 8 cases, and there was no tumor. Head MRI abnormalities in 9 cases, involving both supratentorial and infratentorial, were common in subcortical white matter, brainstem, thalamus and cerebellum. Enhancement scan showed patchy and nodular enhancement, with some linear enhancement of meninges. Spine MRI abnormalities were found in 6 cases, especially in cervical and thoracic spinal cord, which showed long segment spinal cord injury. Optic nerve was involved in 2 cases. Partial or complete remission was achieved after immunomodulatory treatment. The EDSS score decreased by 1-3.50 at discharge. The average follow⁃up was 12.80 months. Three cases recurred and responded well after immunomodulatory treatment again. Conclusions MOGAD complicated with antibody overlap syndrome can detect MOG⁃IgG and other types of autoimmune antibodies at the same time or successively, but it can only show the symptoms of one of the diseases. It has a good response to immunomodulatory treatment, and immunomodulatory treatment is still effective after recurrence.

To reveal the effects of void defects on the diffusivities of plain woven composites, a multiscale analysis framework considering the microscale and mesoscale voids is developed in this study. Different void modeling methods and analysis strategies are used to reveal the effects of void size, void shape, and void modeling assumptions on the equivalent diffusivities of the tows and plain woven composites. The analysis results show that the transverse or longitudinal diffusivities of tows predicted with an element-based modeling method are larger than those with void shapes considered. Considering void shape results in the variation of the predicted diffusivities. Based on the uniform assumption used for modeling the voids in the tows, the through-thickness and in-plane diffusivities are found to be dependent on the transverse and longitudinal diffusivities, respectively. Based on the element-based void modeling method for the tows, the predicted values are larger than those based on the uniform assumption. These tow void modeling methods cannot result in variation within predicted values. The effects of void shapes in the pure matrix on the diffusivities are also revealed, and the variation is observed.

In the traditional ciphertext-policy attribute-based encryption (CP-ABE) scheme,the access policy exists together with the ciphertext.This may leak the privacy of the data owner and bring potential security risks to the data owner in medicalscena-rios Therefore,solutions supporting access policy hiding have been proposed.However,most solutions need to generate redundant ciphertexts or key components in the process of implementing the decryption test,which increases the computing overhead of data owners and the storage overhead of data users.At the same time,malicious users may be motivated by its own interest to reveal their decryption keys.In order to solve the problems above,a lightweight medical data sharing scheme with access policy hiding and key tracking is proposed.Firstly,part of the master key is stored in the Enclave in advance by using software guard extensions(SGX) technology,so that the test results can be calculated accurately and quickly,and the generation of redundant ciphertexts and key components are avoided.Then,verifiable outsourcing technology is employed to reduce user's computing overhead,ensuring the correctness and completeness of decryption result.Finally,key tracking is realized by embedding the identity identifier in the decryption key of the data user.Performance analysis shows that the proposed scheme has certain advantages in terms of function and computing.The security analysis proves that the proposed scheme is secure under the selected plaintext attack.

Taraxasterol (TAX) has been proven to prevent and treat inflammatory diseases. However, the effects of TAX on intestinal barrier and the diversity, structure, and function of gut microbiota have yet to be elucidated in dextran sodium sulfate (DSS)-induced colitis mice. Our objectives are to evaluate the effect of TAX on intestinal barrier and its impact on gut microbiota. Herein, immunofluorescence analysis is conducted to determine the expressions of tight junction (ZO-1) and mucin (Mucin-2) proteins. The abundance, diversity, and function of fecal colonies are investigated by using 16S rDNA sequencing, and the influence of TAX on the gut microbiota in mice is also analyzed. Our results suggest that TAX attenuates the symptoms in DSS-induced colitis mice by reducing the DAI score, increasing colon length, alleviating histopathological damage of colon tissues, and improving intestinal barrier. 16S rDNA sequencing of fecal samples indicates that TAX intervention has a regulatory effect on DSS-induced gut microbiota dysbiosis at different taxonomic levels. TAX increases microbial diversity that is reduced by DSS. It normalizes the relative abundance of Bacteroidetes and the ratio of Bacteroidetes/Firmicutes. In addition, treatment with TAX has a better effect on the function of metabolisms, such as nucleotide, lipid, and bile acid metabolism. These findings suggest that TAX may be a good candidate for the remission of colitis, which is related to improving intestinal barrier and modulating gut microbiota.