Search

Somatic hybrid plants of various ploidy levels obtained after chemical fusion between two dihaploid clones of potato Solanum tuberosum L. have been analysed by cytological, morphological and molecular methods. The hybrid nature of tetraploid and hexaploid plants and the genome dosage in hexaploid hybrids were confirmed by Giemsa C-banding. Tetraploid and hexaploid hybrids showed numerical as well as structural chromosome mutations. The latter occurred mainly in the nuclear organizing chromosome. The tetraploid hybrids were more vigorous than the dihaploid parents as demonstrated by an increase in height, enlargement of leaves, increase in the number of internodes, restored potential for flowering and increased tuber yield. The grouping of tetraploid somatic hybrids into various classes on the basis of leaf morphology revealed that plants with a full chromosome complement were more uniform than aneuploids. Many hexaploid somatic hybrids were also more vigorous than the dihaploid parents and could be grouped into two different classes on the basis of floral colour and tuber characteristics, the differences being due to their different dosage of parental genomes. Most of the tetraploid somatic hybrids showed pollen development halted at the tetrad stage as one of the parental clones contained a S. Stoloniferum cytoplasm. However, one tetraploid plant produced pollen grains with high viability. The chloroplast genome in the hybrid plants was determined by RFLP analysis. All of the hybrids had a cpDNA pattern identical to one parent, which contained either S. Tuberosum or S. Stoloniferum cpDNA. A slight preference for S. Tuberosum plastids were observed in hybrid plants. No correlation between pollen development and plastid type could be detected. [ABSTRACT FROM AUTHOR]

Persistent, mobile, and toxic (PMT) substances have recently garnered increased attention by environmental researchers, the water sector and environmental protection agencies. In this study, acute and chronic species sensitivity distributions (SSDs) were retrieved from literature data for previously quantified contaminants of emerging concern (CECs) in Swedish surface waters (n = 92) and risk quotients (RQ) were calculated. To better understand the characteristics of the detected CECs in non-urban lake sites (n = 71), these compounds were checked against established criteria for potentially toxic PMs (PM(T)s) and occurrence in the aquatic environment, respectively. For the CECs with missing SSDs (n = 15 [acute], n = 41 [chronic]), ecotoxicity data were extracted for eight taxonomic groups, and if data were sufficient (n ≥ 3), SSDs were derived. The retrieved and newly developed SSDs were then used in an environmental hazard assessment (EHA) in the investigated Swedish rivers and lakes. In the rivers, 8 CECs had RQ> 1 in at least one location, and 20 CECs posed a moderate risk (0.01 < RQ < 1). In total, 21 of the 71 detected substances had already been identified as PM(T)/vPvM substances. Our study shows the importance of studying field data at large spatial scale to reveal potential environmental hazards far from source areas., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Concerns about environmental contamination by organic micropollutants (OMPs) are increasing, due to their potential bioaccumulative and toxic properties. This study evaluated the risk posed by OMPs to aquatic ecosystems in Swedish freshwaters. The assessment was based on measured environmental concentrations (MEC) of OMPs in surface waters upstream and downstream of Swedish wastewater treatment plants (WWTPs). A novel optimised risk quotient (RQ f ) was used to identify potential high-risk substances in the aquatic environment. A secondary objective was to assess the impact of WWTP effluent on aquatic ecosystems using a novel impact factor (I) based on the risk quotient (RQ). Among the 126 substances investigated, four compounds (metformin, N,N-dimethyltetradecylamine, oxazepam, and venlafaxine) were identified as likely to pose a risk to aquatic ecosystems in Swedish surface waters (RQ f >1), and five compounds (clindamycin, gemfibrozil, sertraline, o-desmethylvenlafaxine, and diclofenac) were identified as posing a moderate risk to aquatic ecosystems ( 0.1 f <1). WWTP effluent appeared to pose an environmental risk for all recipient sites, but the impact of calculated RQ was site-specific. These results can be used by authorities to prioritise OMPs and contaminated hotspots, in order to decrease negative impacts on aquatic ecosystems. SYNOPSIS: A novel optimised risk assessment approach for identification of high-concern organic micropollutants in aquatic environments., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

Stormwater ponds can provide flood protection and efficiently treat stormwater using sedimentation. As the ponds also host aquatic biota and attract wildlife, there is a growing concern that the sediment bound pollutants negatively affect aquatic organisms and the surrounding ecosystem. In this study, we used three methods to assess the accumulation and the potential ecological risk of 13 different heavy metals and metalloids (e.g. trace elements) including both elements that are frequently monitored and some which are rarely monitored in sediment from 5 stormwater ponds located within catchments with predominately industrial activities. Ecological risk for organisms in the older ponds was observed for both commonly (e.g. Cd, Cu, Zn) and seldom (e.g. Ag, Sb) monitored trace elements. The 3 methods ranked the degree of contamination similarly. We show that methods usually used for sediment quality assessment in aquatic ecosystems can also be used for screening the potential risk of other trace elements in stormwater ponds and may consequently be useful in stormwater monitoring and management. Our study also highlights the importance of establishing background conditions when conducting ecological risk assessment of sediment in stormwater ponds., (© 2021. The Author(s).)

An 8 ha free water surface wetland system in Örebro, Sweden, which has sediment traps followed by 10 ponds for treatment of landfill leachate in the methanogenic stage, was studied from 2003 to 2012. The wetland was irregularly loaded and the leachate characteristics showed high temporal and spatial variability, so Multivariate Statistical Process Control was chosen as the diagnostic tool for detecting anomalies prior to assessing removal efficiency for eutrophying substances and organics. Disturbances in process behaviour included those due to intermittent flow, dilution due to snowmelt, an episode of high pollutant load, and sampling technique. The wetland total nitrogen mass removal was 89%, resulting in a first order rate coefficient of 1.7 m/year. Total phosphorus mass removal was 98%, while mass reduction was lower for TOC (60%), although this was still below discharge limits. The low amount of labile organic material and phosphate are probably the main reasons for the low denitrification rate., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

In this study the influence of environmental conditions, most likely prevailing in filter beds used for intermittently discharged pollutant streams such as landfill leachate and storm water, on the stability of the heavy metal-filter complex was investigated for 2 filter materials; non-treated and urea treated pine bark, using leaching experiments. The metal-filter complex stability was higher for urea treated than for non-treated pine bark and dependent on the metal adsorbed. The type of environmental condition applied was of less importance for the extent of leaching., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

The results of heavy metals (Fe, Mn, Zn, Ni, Cu, Cr, Pb, Cd) removal and partitioning between aqueous and solid phases at two treatment wetlands (TWs) treating municipal landfill leachates are presented. One of the TWs is a surface flow facility consisting of 10 ponds. The other TW is a newly constructed pilot-scale facility consisting of three beds with alternately vertical and horizontal subsurface flow. The metals concentrations were analysed in leachate (both TWs) and bottom sediments (surface flow TW). Very high (90.9-99.9%) removal rates of metals were observed in a mature surface flow TW. The effectiveness of metals removal in a newly constructed pilot-scale sub-surface flow wetland were considerably lower (range 0-73%). This is attributed to young age of the TW, different hydraulic conditions (sub-surface flow system with much shorter retention time, unoxic conditions) and presence of metallic complexes with refractory organic matter.

A previous study showed considerably higher metal adsorption by urea-treated pine bark (UTB) compared to non-treated bark (NTB) at metal adsorption from their individual relatively concentrated solutions. Comparison of the sorption characteristics of the two pine barks at low but environmentally relevant metal concentrations, and investigation of the influence of pH and dissolved organic carbon (DOC) on the sorption process are the aims of the present study. Sorption of Cu(2+), Ni(2+), Zn(2+) and Pb(2+) on pine bark of the species Pinus sylvestris was measured in multi-metal solutions in the presence and absence of DOC. In the absence of DOC, UTB gave lower residual metal concentrations (2-7 microg/l for copper, 1-5 microg/l for nickel, <0.05 microg/l for zinc and lead) in the range of initial concentrations up to 0.7 mg/l, compared to NTB (6-15 microg/l for copper, 2-24 microg/l for nickel, 2-9 microg/l for zinc, 2-3 microg/l for lead). In the presence of DOC, sorption of Zn, Ni and Pb decreased by up to 75% depending on the DOC concentration. Metal sorption on UTB is less sensitive to pH and more adsorbed metal ions are retained compared to NTB. The potential use of urea-treated bark for treatment of waste water containing DOC and low concentrations of metals is discussed.

Soil washing of a soil with a mixture of both polycyclic aromatic hydrocarbons (PAH) and As was evaluated in laboratory and pilot scale, utilizing both single and mixtures of different additives. The highest level of decontamination was achieved with a combination of 0.213 M of the chelating agent MGDA and 3.2 x CMC* of a non-ionic, alkyl glucoside surfactant at pH 12 (Ca(OH)(2)). This combination managed to reach Swedish threshold values within 1 0 min of treatment when performed at elevated temperature (50 degrees C), with initial contaminant concentrations of As=105+/-4 mg/kg and US-EPA PAH(16)=46.0+/-2.3mg/kg. The main mechanisms behind the removal were the pH effect for As and a combination of SOM ionization as a result of high pH and micellar solubilization for PAHs. Implementation of the laboratory results utilizing a pilot scale equipment did not improve the performance, which may be due to the shorter contact time between the washing solution and the particles, or changes in physical characteristics of the leaching solution due to the elevated pressure utilized. The ecotoxicological evaluation, Microtox, demonstrated that all soil washing treatments increased the toxicity of soil leachates, possibly due to increased availability of contaminants and toxicity of soil washing solutions to the test organism.

Low cost sorbents have been widely studied in recent years in the search for filter materials that retain contaminants from water. One promising, low cost material is pine bark, a by-product from the forest industry. Many studies have shown that pine bark has great potential for the treatment of metals and organic substances, as a replacement for other commercial sorbents such as active carbon. However, some potential problems are introduced through the use of natural materials and by-products. One such problem that must be addressed is the possibility of leaching of contaminants from the filter material, especially in the initial filtration step or during flushes of lightly contaminated water, e.g. during rainfall for on-site treatment of storm water or landfill leachate. The aim of this preliminary study was therefore to identify potential risks and limitations of using pine bark as a filter material. Leachate from a standardized batch test was analysed for metals, dissolved organic carbon (DOC) and phenols. In addition to these chemical analyses, an ecotoxicological test was conducted using the test organism Daphnia magna. The results showed significant leaching of DOC and some metals. Only a small fraction of the DOC was present as phenols. The leachate was however found to be toxic to the test organism without pH adjustment, and the EC(50) was established at an approximate leachate concentration of 40%. This was concluded to be related to the low pH in the eluate, since no toxicity was observed after pH adjustment before the toxicity tests.

Goal, Scope and Background: The potential negative impact of urban storm water on aquatic freshwater ecosystems has been demonstrated in various studies with different types of biological methods. There are a number of factors that influence the amount and bioavailability of contaminants in storm water even if it is derived from an area with a fairly homogenous land use such as a roadscape where a variation in toxicity during rain events might be expected. There are only a few previous investigations on the toxicity of highway runoff and they have not explored these issues extensively. The main objective of this study is therefore to characterize the potential toxicity of highway runoff during several rain events before it enters a detention pond in Västerås, Sweden, using laboratory bioassays with test organisms representing various functional groups in an aquatic ecosystem. The results are to be used for developing a monitoring program, including biological methods., Materials and Methods: The storm water was sampled before the entrance to a detention pond, which receives run-off from a highway with approximately 20,000 vehicles a day. The drainage area, including the roadscape and vegetated areas, is 4.3 ha in size. Samples for toxicity tests were taken with an automatic sampler or manually during storm events. In total, the potential toxicity of 65 samples representing 15 different storm events was determined. The toxicity was assessed with 4 different test organisms; Vibrio fischeri using the Microtox comparison test, Daphnia magna using Daphtoxkit-F agna, Thamnocephalus platyurus using the ThamnotoxkitF and Lemna minor, duckweed using SS 028313., Results and Discussion: Of the 65 samples, 58 samples were tested with DaphniatoxkitF agna, 57 samples with the Microtox comparison test, 48 samples with ThamnotoxkitF and 20 samples with Lemna minor, duckweed. None of the storm water samples were toxic. No toxicity was detected with the Lemna minor test, but in 5 of the 23 samples tested in comparison to the control a growth stimulation of 22-46% was observed. This is in accordance with the chemical analysis of the storm water, which indicated rather large concentrations of tot-N and tot-P. In addition to the growth stimulation, morphological changes were observed in all the 5 samples from the winter event that was sampled. The lack of toxicity observed in our study might be due to a lower traffic intensity (20,000 vehicles/day) at the site and the trapping of pollutants in the vegetated areas of the roadscape, resulting in much smaller loads of pollutants in the storm water than in some previous studies., Conclusions: Ecotoxicological evaluations of storm water including run off from rain events from urban roadscape studies clearly reveal that toxicity may or may not be detected depending upon site, storm condition and the test organism chosen. However, storm water might not be as polluted as previously reported nor may the first flush be such a widespread phenomenon as we originally expected. In this study, there was also a good correlation between pollutant load measured and the lack of toxicity. The test organisms chosen in this study are commonly used in effluent control programs in Sweden and other countries, which makes it possible to compare the results with those from other effluents. In this study, only acute toxicity tests were used and further studies using chronic toxicity tests, assays for genotoxic compounds or in situ bioassays might reveal biological effects at this site. Furthermore, most of the samples were taken in spring, summer or fall and it is possible that winter conditions might alter the constituents in the storm water and, thus, the toxicity of the samples., Recommendations and Perspectives: Considering the complex nature of run off from urban roadscapes, it will be virtually impossible to evaluate properly the potential hazard of particular storm water and the efficiency of a particular treatment strategy from only physical and chemical characterizations of the effluent. Therefore, despite the lack of toxicity detected in this study, it is recommended that toxicity tests or other biological methods should be included in evaluations of the effects of runoff from roadscapes.

Treatment of landfill leachate using blast furnace slag and pine bark as reactive sorbents was studied in an in situ column experiment at the Lilla Nyby landfill site in Eskilstuna, Sweden. The columns were filled with approximately 101 of each sorbent and leachate was supplied at three different flow rates during a period of 4 months. Samples of inflow and outflow were collected three times a week and were analyzed for physical and chemical parameters, including concentrations of some metals, and toxicity. It was found that pine bark removed metals more efficiently than did the blast furnace slags; that Zn was most efficiently retained in the filters and that both retention time and initial concentration played an important role in the sorption process. It was also observed that the pine bark column did not release COD. No toxicity of the untreated or the treated leachate was found with the test organisms and test responses used.