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46 results on '"Wachtel, Chaim"'

10. Additional file 8 of A comparative genome-wide study of ncRNAs in trypanosomatids

Author

Doniger, Tirza, Katz, Rodolfo, Wachtel, Chaim, Michaeli, Shulamit, and Unger, Ron

Abstract

Additional file 8:Effects of NOP58 and CBF5 knockdown on all candidates. RNA was prepared from cells carrying either NOP58 or CBF5 silencing constructs before induction with Tetracycline (-) and 3 days after addition of tetracycline (+). The RNA was analyzed by primer extension and separated on a 6% denaturing polyacrylamide gel. The level of U6 snRNA was used to examine the amount of RNA the samples. The same RNA was used for the different primer extension assays. (PPT 386 KB)

Published
2021
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15. Projection of Gut Microbiome Pre- and Post-Bariatric Surgery To Predict Surgery Outcome

Author

Ben Izhak, Meirav, primary, Eshel, Adi, additional, Cohen, Ruti, additional, Madar-Shapiro, Liora, additional, Meiri, Hamutal, additional, Wachtel, Chaim, additional, Leung, Conrad, additional, Messick, Edward, additional, Jongkam, Narisra, additional, Mavor, Eli, additional, Sapozhnikov, Shimon, additional, Maharshak, Nitsan, additional, Abu-Abeid, Subhi, additional, Alis, Avishai, additional, Mahler, Ilanit, additional, Meoded, Aviel, additional, Meron Eldar, Shai, additional, Koren, Omry, additional, and Louzoun, Yoram, additional

Published
2021
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18. Projection of gut microbiome pre and post-bariatric surgery to predict surgery outcome

Author

Izhak, Meirav Ben, primary, Eshel, Adi, additional, Cohen, Ruti, additional, Shapiro, Liora Madar, additional, Meiri, Hamutal, additional, Wachtel, Chaim, additional, Leung, Conrad, additional, Messick, Edward, additional, Jongkam, Narisra, additional, Mavor, Eli, additional, Sapozhnikov, Shimon, additional, Maharshak, Nitsan, additional, Abu-Abeid, Subhi, additional, Alis, Avishai, additional, Mahler, Ilanit, additional, Meoded, Aviel, additional, Eldar, Shai Meron, additional, Koren, Omry, additional, and Louzoun, Yoram, additional

Published
2020
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22. Projection of gut microbiome pre and post bariatric surgery to predict surgery outcome

Author

louzoun, yoram, primary, Izhak, Meirav Ben, additional, Cohen, Ruti, additional, Shapiro, Liora Madar, additional, Meiri, Hamutal, additional, Wachtel, Chaim, additional, Eshel, Adi, additional, Leung, Conrad, additional, Messick, Edward, additional, Jongkam, Narisra, additional, Mavor, Eli, additional, Sapozhnikov, Shimon, additional, Maharshak, Nitsan, additional, Abu-Abeid, Subhi, additional, Alis, Avishai, additional, Mahler, Ilanit, additional, Meoded, Aviel, additional, Eldar, Shai, additional, and Koren, Omry, additional

Published
2019
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24. Evaluating methods for Avian avulavirus-1 whole genome sequencing

Author

Tal, Saar, primary, Ben Izhak, Meirav, additional, Wachtel, Chaim, additional, Wiseman, Anat, additional, Braun, Tzipi, additional, Yechezkel, Elinor, additional, Golan, Einav, additional, Hadas, Ruth, additional, Turjeman, Adi, additional, Banet-Noach, Caroline, additional, Bronstein, Michal, additional, Lublin, Avishai, additional, Berman, Elyakum, additional, Raviv, Ziv, additional, Pirak, Michael, additional, Klement, Eyal, additional, and Louzoun, Yoram, additional

Published
2019
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31. Drosophila TRF2 is a preferential core promoter regulator

Author

Kedmi, Adi, primary, Zehavi, Yonathan, additional, Glick, Yair, additional, Orenstein, Yaron, additional, Ideses, Diana, additional, Wachtel, Chaim, additional, Doniger, Tirza, additional, Waldman Ben-Asher, Hiba, additional, Muster, Nemone, additional, Thompson, James, additional, Anderson, Scott, additional, Avrahami, Dorit, additional, Yates, John R., additional, Shamir, Ron, additional, Gerber, Doron, additional, and Juven-Gershon, Tamar, additional

Published
2014
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33. Identification of novel transcriptional regulators of Zat12 using comprehensive yeast one-hybrid screens.

Author

Ben Daniel, Bat‐Hen, Cattan, Esther, Wachtel, Chaim, Avrahami, Dorit, Glick, Yair, Malichy, Asaf, Gerber, Doron, and Miller, Gad

Subjects
ENVIRONMENTAL engineering, YEAST-free diet, ENVIRONMENTAL protection, ABIOTIC stress, THIGMOMORPHOGENESIS
Abstract

To appropriately acclimate to environmental stresses, plants have to rapidly activate a specific transcriptional program. Yet, the identity and function of many of the transcriptional regulators that mediate early responses to abiotic stress stimuli is still unknown. In this work we employed the promoter of the multi-stress-responsive zinc-finger protein Zat12 in yeast one-hybrid ( Y1H) screens to identify early abiotic stress-responsive transcriptional regulators. Analysis of Zat12 promoter fragments fused to luciferase underlined an approximately 200 bp fragment responsive to NaCl and to reactive oxygen species ( ROS). Using these segments and others as baits against Y1H control or stress Arabidopsis prey libraries, we identified 15 potential Zat12 transcriptional regulators. Among the prominent proteins identified were known transcription factors including bZIP29 and ANAC91 as well as unknown function proteins such as a homolog of the human USB1, a U6 small nuclear RNA ( snRNA) processing protein, and dormancy/auxin-associated family protein 2 ( DRM2). Altered expression of Zat12 during high light stress in the knockout mutants further indicated the involvement of these proteins in the regulation of Zat12. Using a state of the art microfluidic approach we showed that AtUSB1 and DRM2 can specifically bind dsDNA and were able to identify the preferred DNA-binding motif of all four proteins. Overall, the proteins identified in this work provide an important start point for charting the earliest signaling network of Zat12 and of other genes required for acclimation to abiotic stresses. [ABSTRACT FROM AUTHOR]

Published
2016
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40. Reduced levels of protein recoding by A-to-I RNA editing in Alzheimer's disease

Author

Khermesh, Khen, D'Erchia, Anna Maria, Barak, Michal, Annese, Anita, Wachtel, Chaim, Levanon, Erez Y., Picardi, Ernesto, and Eisenberg, Eli

Abstract

Adenosine to inosine (A-to-I) RNA editing, catalyzed by the ADAR enzyme family, acts on dsRNA structures within pre-mRNA molecules. Editing of the coding part of the mRNA may lead to recoding, amino acid substitution in the resulting protein, possibly modifying its biochemical and biophysical properties. Altered RNA editing patterns have been observed in various neurological pathologies. Here, we present a comprehensive study of recoding by RNA editing in Alzheimer's disease (AD), the most common cause of irreversible dementia. We have used a targeted resequencing approach supplemented by a microfluidic-based high-throughput PCR coupled with next-generation sequencing to accurately quantify A-to-I RNA editing levels in a preselected set of target sites, mostly located within the coding sequence of synaptic genes. Overall, editing levels decreased in AD patients’ brain tissues, mainly in the hippocampus and to a lesser degree in the temporal and frontal lobes. Differential RNA editing levels were observed in 35 target sites within 22 genes. These results may shed light on a possible association between the neurodegenerative processes typical for AD and deficient RNA editing.

Published
2016
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41. Stop codons affects 5' splice site selection by surveillance of splicing.

Author

Binghui Li, Wachtel, Chaim, Yahalom, Galit, Friedlander, Gilgi, Sharon, Gil, Sperling, Ruth, and Sperling, Joseph

Subjects
*BIOCHEMISTRY, *RNA splicing, *RNA polymerases
Abstract

Examines the stop codon in the splice site selection by surveillance of splicing. Accuracy of mRNA splicing with transacting factors; Activation of latent splicing with splicing control elements; Discussion on the relevance of accurate gene expression for protein-coding transcripts of RNA polymerase.

Published
2002

42. Additional file 5 of A comparative genome-wide study of ncRNAs in trypanosomatids

Author

Doniger, Tirza, Katz, Rodolfo, Wachtel, Chaim, Michaeli, Shulamit, and Unger, Ron

Subjects
education, health care economics and organizations, 3. Good health
Abstract

Additional file 5:Results of expressed candidates in primer extension assay with an internal control. Primer extension was performed as in Figure 2 with the addition of an internal control to each sample. The primer extension reactions contained a primer specific for the candidate as well as a primer specific to U3 snoRNA. (PPT 519 KB)

44. Additional file 5 of A comparative genome-wide study of ncRNAs in trypanosomatids

Author

Doniger, Tirza, Katz, Rodolfo, Wachtel, Chaim, Michaeli, Shulamit, and Unger, Ron

Subjects
education, health care economics and organizations, 3. Good health
Abstract

Additional file 5:Results of expressed candidates in primer extension assay with an internal control. Primer extension was performed as in Figure 2 with the addition of an internal control to each sample. The primer extension reactions contained a primer specific for the candidate as well as a primer specific to U3 snoRNA. (PPT 519 KB)

46. Synthesis and evaluation of a photoresponsive quencher for fluorescent hybridization probes.

Author

Kovaliov M, Wachtel C, Yavin E, and Fischer B

Subjects
Chemistry Techniques, Synthetic, Deoxyuridine chemistry, Isomerism, Nucleic Acid Hybridization, Oligonucleotides chemistry, Organophosphorus Compounds chemistry, p-Dimethylaminoazobenzene chemical synthesis, p-Dimethylaminoazobenzene chemistry, Fluorescent Dyes chemical synthesis, Fluorescent Dyes chemistry, Photochemical Processes, p-Dimethylaminoazobenzene analogs & derivatives
Abstract

Nowadays, most nucleic acid detections using fluorescent probes rely on quenching of fluorescence by energy transfer from one fluorophore to another or to a non-fluorescent molecule (quencher). The most widely used quencher in fluorescent probes is 4-((4-(dimethylamino)phenyl)azo)benzoic acid (DABCYL). We targeted a nucleoside-DABCYL analogue which could be incorporated anywhere in an oligonucleotide sequence and in any number, and used as a quencher in different hybridization sensitive probes. Specifically, we introduced a 5-(4-((dimethylamino)phenyl)azo)benzene)-2'-deoxy-uridine (dU(DAB)) quencher. The photoisomerization and dU(DAB)'s ability to quench fluorescein emission have been investigated. We incorporated dU(DAB) into a series of oligonucleotide (ON) probes including strand displacement probes, labeled with both fluorescein (FAM) and dU(DAB), and TaqMan probes bearing one or two dU(DAB) and a FAM fluorophore. We used these probes for the detection of a DNA target in real-time PCR (RT-PCR). All probes showed amplification of targeted DNA. A dU(DAB) modified TaqMan RT-PCR probe was more efficient as compared to a DABCYL bearing probe (93% vs. 87%, respectively). Furthermore, dU(DAB) had a stabilizing effect on the duplex, causing an increase in Tm up to 11 °C. In addition we showed the photoisomerisation of the azobenzene moiety of dU(DAB) and the dU(DAB) triply-labeled oligonucleotide upon irradiation. These findings suggest that dU(DAB) modified probes are promising probes for gene quantification in real-time PCR detection and as photoswitchable devices.

Published
2014
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