1. In vitro antigenotoxicity of ulva rigida c. agardh (chlorophyceae) extract against induction of chromosome aberration, sister chromatid exchange and micronuclei by mutagenic agent MMC
- Author
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Gamze Yildiz, Serap Celikler, Rahmi Bilaloglu, Ozgur Vatan, Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Çelikler, Serap, Yıldız, Gamze, Vatan, Özgür, Bilaloǧlu, Rahmi, AAH-2767-2021, O-7508-2015, and A-9944-2010
- Subjects
Health, Toxicology and Mutagenesis ,Cells ,Marine-algae ,Sister chromatid exchange ,Biology ,Micronuclei ,Chromosome aberration ,Chromosomal aberration ,Antioxidants ,Mitomycins ,Natural-products ,Clastogen ,Ulva ,Chlorophyta ,Chlorophyceae ,Environmental sciences & ecology ,Humans ,Lymphocytes ,Anticlastogenicity ,Cells, Cultured ,Purification ,Genetics ,Chromosome Aberrations ,Antibiotics, Antineoplastic ,Micronucleus Tests ,Dose-Response Relationship, Drug ,Plant Extracts ,Seaweeds ,Padina ,Mitomycin C ,Carcinoma ,Public Health, Environmental and Occupational Health ,Antimutagenic Agents ,Antigenotoxicity ,Seaweed ,Molecular biology ,Ulva rigida ,Carotenoids ,In vitro ,Environmental sciences ,Dose–response relationship ,Cell culture ,Micronucleus test ,Mitomycin-C ,Antibacterial activity ,Wakame undaria-pinnatifida ,Public, environmental & occupational health ,Mutagens - Abstract
Objective To determine the in vitro possible clastogenic and cytotoxic activities of Ova rigida crude extracts (URE), and identify their antigenotoxic and protective effects on chemotherapeutic agent mitomycine-C (MMC). Methods Anti-clastogenic and anti-genotoxic activities of Ulva rigida crude extracts (URE) were studied using chromosome aberration (CA), sister chromatid exchange (SCE), and micronuclei (MN) tests in human lymphocytes cultured in vitro. Results The chromosome aberration, sister chromatid exchange or micronuclei tests showed that URE at concentrations of 10, 20, and 40 mu g/mL had no clastogenic activity in human lymphocyte cell Culture. Three doses of URE significantly decreased the number of chromosomal aberrations and the frequencies of SCE and MN when compared with the culture treated with MMC (P
- Published
- 2008