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5 results on '"Wakana Sugitani"'

1. Non-biogroup 1 or 2 Strains of the Emerging Zoonotic Pathogen Escherichia albertii, Their Proposed Assignment to Biogroup 3, and Their Commonly Detected Characteristics

Author

Koichi Murakami, Eriko Maeda-Mitani, Hirokazu Kimura, Mikiko Honda, Tetsuya Ikeda, Wakana Sugitani, Takayuki Konno, Kimiko Kawano, Yoshiki Etoh, Nobuyuki Sera, Fuminori Mizukoshi, Takehito Saitoh, Yoshiaki Kawamura, Taisei Ishioka, Makoto Ohnishi, Kazunori Oishi, and Shuji Fujimoto

Subjects
biogroups, Escherichia albertii, food microbiology, identification, public health, Microbiology, QR1-502
Abstract

Escherichia albertii, a zoonotic enteropathogen, is responsible for outbreaks of disease in humans. Identifying strains of E. albertii by phenotypic characterization tests is difficult because of its poorly defined properties. Screening its phenotypic characteristics is, nevertheless, a necessary prerequisite for further genetic analysis of its properties, and species-specific polymerase chain reaction (PCR) analysis can be used to type the pathogen. While two E. albertii biogroups (1 and 2) have been described, strains with characteristics divergent from both biogroups have been reported worldwide. The aim of the present study was to evaluate the characteristics of non-biogroup 1 or 2 strains, and discern the characteristics common to all of the E. albertii strains from this study. Altogether, 107/414 field isolates were selected for examination based on pulsed-field gel electrophoresis analysis. The 107 strains were isolated from 92 sources, including humans and pigeon feces, other wild birds, and retail chicken livers. All strains were then examined using various culture-based, biochemical (API 50CHE tests, API Zym test, and others) and molecular (virulence gene screening, multi-locus sequence analysis) testing methods. Our results revealed that all field strains (n = 107) showed non-biogroup 1 or 2 characteristics, with multiple sequence differences. Variations in indole production and the lysine decarboxylase activity profiles among the isolates made identification of E. albertii very difficult. Therefore, we propose that non-biogroup 1 or 2 of E. albertii should be assigned to biogroup 3 to make screening of them easier in public health and clinical laboratory settings. Clearly, having group criteria for indole-negative/lysine-positive, indole-positive/lysine-negative, and indole-positive/lysine-positive E. albertii biogroups 1, 2, and 3 strains, respectively, should provide for more accurate identification of E. albertii isolates. Based on our findings, we recommend that isolates displaying phenotype mobility-negativity (sulfide-indole-motility medium, 37°C), hydrogen sulfide production-negativity (triple sugar iron medium), acid production-negativity from xylose, negative β-glucuronidase activity properties, and showing indole production and lysine decarboxylase activity profiles in accordance with one of the three biogroups, should be further assessed using an E. albertii-specific PCR assay.

Published
2019
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2. Epidemiological Aspects of Escherichia albertii Outbreaks in Japan and Genetic Characteristics of the Causative Pathogen

Author

Wakana Sugitani, Kazunori Oishi, Yukio Morita, Junko Toda, Takahiro Hiratsuka, Hiroko Akita, Makoto Kuroda, Taisei Ishioka, Hiroshi Narimatsu, Makoto Ohnishi, Mami Fukada, Kaori Inoue, Shinichiro Hirai, Koichi Murakami, Shuji Fujimoto, Tsuyoshi Sekizuka, Kanako Masuda, Tetsuya Hayashi, Shinichi Takao, Mikiko Honda, Hirokazu Kimura, and Tadasuke Ooka

Subjects
0303 health sciences, medicine.medical_specialty, biology, 030306 microbiology, 040301 veterinary sciences, General symptoms, Outbreak, macromolecular substances, 04 agricultural and veterinary sciences, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, Incubation period, Escherichia albertii, 0403 veterinary science, 03 medical and health sciences, Diarrhea, Epidemiology, medicine, Animal Science and Zoology, medicine.symptom, Pathogen, Zoonotic pathogen, Food Science
Abstract

Zoonotic pathogen Escherichia albertii has been identified as the cause of several human disease outbreaks; however, factors such as the general symptoms and incubation period of E. albertii infect...

Published
2020
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3. O-antigen biosynthesis gene clusters of

Author

Tadasuke, Ooka, Kazuko, Seto, Yoshitoshi, Ogura, Keiji, Nakamura, Atsushi, Iguchi, Yasuhiro, Gotoh, Mikiko, Honda, Yoshiki, Etoh, Tetsuya, Ikeda, Wakana, Sugitani, Takayuki, Konno, Kimiko, Kawano, Naoko, Imuta, Kiyotaka, Yoshiie, Yukiko, Hara-Kudo, Koichi, Murakami, Tetsuya, Hayashi, and Junichiro, Nishi

Subjects
Escherichia, Base Sequence, Genotype, O Antigens, Systems Microbiology: Large-scale comparative genomics, phylogeny, Escherichia albertii, genotyping, Multigene Family, O-antigen gene cluster, Escherichia coli, Humans, Serotyping, genome, Genome, Bacterial, Research Article
Abstract

Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli . In many Gram-negative bacteria, including E. coli , O-antigen variation has long been used for the serotyping of strains. In E. albertii , while eight O-serotypes unique to this species have been identified, some strains have been shown to exhibit genetic or serological similarity to known E. coli / Shigella O-serotypes. However, the diversity of O-serotypes and O-antigen biosynthesis gene clusters (O-AGCs) of E. albertii remains to be systematically investigated. Here, we analysed the O-AGCs of 65 E. albertii strains and identified 40 E. albertii O-genotypes (EAOgs) (named EAOg1–EAOg40). Analyses of the 40 EAOgs revealed that as many as 20 EAOgs exhibited significant genetic and serological similarity to the O-AGCs of known E. coli / Shigella O-serotypes, and provided evidence for the inter-species horizontal gene transfer of O-AGCs between E. albertii and E. coli . Based on the sequence variation in the wzx gene among the 40 EAOgs, we developed a multiplex PCR-based O-genotyping system for E. albertii (EAO-genotyping PCR) and verified its usefulness by genotyping 278 E. albertii strains from various sources. Although 225 (80.9 %) of the 278 strains could be genotyped, 51 were not assigned to any of the 40 EAOgs, indicating that further analyses are required to better understand the diversity of O-AGCs in E. albertii and improve the EAO-genotyping PCR method. A phylogenetic view of E. albertii strains sequenced so far is also presented with the distribution of the 40 EAOgs, which provided multiple examples for the intra-species horizontal transfer of O-AGCs in E. albertii .

Published
2019

4. O-antigen biosynthesis gene clusters of Escherichia albertii: their diversity and similarity to Escherichia coli gene clusters and the development of an O-genotyping method

Author

Tetsuya Hayashi, Kazuko Seto, Mikiko Honda, Koichi Murakami, Keiji Nakamura, Junichiro Nishi, Wakana Sugitani, Kimiko Kawano, Yoshitoshi Ogura, Naoko Imuta, Atsushi Iguchi, Takayuki Konno, Yasuhiro Gotoh, Yukiko Hara-Kudo, Tadasuke Ooka, Yoshiki Etoh, Kiyotaka Yoshiie, and Tetsuya Ikeda

Subjects
Genetics, biology, Phylogenetic tree, General Medicine, medicine.disease_cause, biology.organism_classification, Genome, Escherichia albertii, Phylogenetics, Horizontal gene transfer, medicine, Shigella, Genotyping, Escherichia coli
Abstract

Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli . In many Gram-negative bacteria, including E. coli , O-antigen variation has long been used for the serotyping of strains. In E. albertii , while eight O-serotypes unique to this species have been identified, some strains have been shown to exhibit genetic or serological similarity to known E. coli / Shigella O-serotypes. However, the diversity of O-serotypes and O-antigen biosynthesis gene clusters (O-AGCs) of E. albertii remains to be systematically investigated. Here, we analysed the O-AGCs of 65 E. albertii strains and identified 40 E. albertii O-genotypes (EAOgs) (named EAOg1–EAOg40). Analyses of the 40 EAOgs revealed that as many as 20 EAOgs exhibited significant genetic and serological similarity to the O-AGCs of known E. coli / Shigella O-serotypes, and provided evidence for the inter-species horizontal gene transfer of O-AGCs between E. albertii and E. coli . Based on the sequence variation in the wzx gene among the 40 EAOgs, we developed a multiplex PCR-based O-genotyping system for E. albertii (EAO-genotyping PCR) and verified its usefulness by genotyping 278 E. albertii strains from various sources. Although 225 (80.9 %) of the 278 strains could be genotyped, 51 were not assigned to any of the 40 EAOgs, indicating that further analyses are required to better understand the diversity of O-AGCs in E. albertii and improve the EAO-genotyping PCR method. A phylogenetic view of E. albertii strains sequenced so far is also presented with the distribution of the 40 EAOgs, which provided multiple examples for the intra-species horizontal transfer of O-AGCs in E. albertii .

Published
2019
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5. Epidemiological Aspects of

Author

Kanako, Masuda, Tadasuke, Ooka, Hiroko, Akita, Takahiro, Hiratsuka, Shinichi, Takao, Mami, Fukada, Kaori, Inoue, Mikiko, Honda, Junko, Toda, Wakana, Sugitani, Hiroshi, Narimatsu, Taisei, Ishioka, Shinichiro, Hirai, Tsuyoshi, Sekizuka, Makoto, Kuroda, Yukio, Morita, Tetsuya, Hayashi, Hirokazu, Kimura, Kazunori, Oishi, Makoto, Ohnishi, Shuji, Fujimoto, and Koichi, Murakami

Subjects
Escherichia, Foodborne Diseases, Genotype, Japan, Virulence Factors, Waterborne Diseases, Enterobacteriaceae Infections, Type III Secretion Systems, Humans, Genome, Bacterial, Phylogeny, Disease Outbreaks
Abstract

Zoonotic pathogen

Published
2019

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