1. Neutrophils promote CXCR3-dependent itch in the development of atopic dermatitis.
- Author
-
Walsh, Carolyn M, Hill, Rose Z, Schwendinger-Schreck, Jamie, Deguine, Jacques, Brock, Emily C, Kucirek, Natalie, Rifi, Ziad, Wei, Jessica, Gronert, Karsten, Brem, Rachel B, Barton, Gregory M, and Bautista, Diana M
- Subjects
Neutrophils ,Cell Line ,Keratinocytes ,Skin ,Animals ,Mice ,Inbred C57BL ,Humans ,Dermatitis ,Atopic ,Pruritus ,Disease Models ,Animal ,Calcitriol ,Cytokines ,Gene Expression Profiling ,Gene Expression Regulation ,Chemokine CXCL10 ,Receptors ,CXCR3 ,Sensory Receptor Cells ,atopic dermatitis ,chronic itch ,immunology ,inflammation ,itch ,mouse ,neuroimmune ,neuroscience ,neutrophils ,somatosensory ,Mice ,Inbred C57BL ,Dermatitis ,Atopic ,Disease Models ,Animal ,Receptors ,CXCR3 ,Biochemistry and Cell Biology - Abstract
Chronic itch remains a highly prevalent disorder with limited treatment options. Most chronic itch diseases are thought to be driven by both the nervous and immune systems, but the fundamental molecular and cellular interactions that trigger the development of itch and the acute-to-chronic itch transition remain unknown. Here, we show that skin-infiltrating neutrophils are key initiators of itch in atopic dermatitis, the most prevalent chronic itch disorder. Neutrophil depletion significantly attenuated itch-evoked scratching in a mouse model of atopic dermatitis. Neutrophils were also required for several key hallmarks of chronic itch, including skin hyperinnervation, enhanced expression of itch signaling molecules, and upregulation of inflammatory cytokines, activity-induced genes, and markers of neuropathic itch. Finally, we demonstrate that neutrophils are required for induction of CXCL10, a ligand of the CXCR3 receptor that promotes itch via activation of sensory neurons, and we find that that CXCR3 antagonism attenuates chronic itch.
- Published
- 2019