1. Protective effects of tanshinone IIA on SH-SY5Y cells against oAβ1–42-induced apoptosis due to prevention of endoplasmic reticulum stress
- Author
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Li-Li Shi, Yi-Hua Qian, Shengfeng Ji, Wan-Ying Zhai, Jianshui Zhang, Wei-Na Yang, Xiaohua Yang, and Hang-Fan Zong
- Subjects
0301 basic medicine ,biology ,ATF6 ,Chemistry ,Kinase ,Glucose-regulated protein ,Endoplasmic reticulum ,Cytochrome c ,Cell Biology ,Mitochondrion ,Biochemistry ,Molecular biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Apoptosis ,030220 oncology & carcinogenesis ,Unfolded protein response ,biology.protein - Abstract
Endoplasmic reticulum (ER) stress caused by β-amyloid protein (Aβ) may play an important role in the pathogenesis of Alzheimer disease (AD). Our previous data have indicated that tanshinone IIA (tan IIA) protected primary neurons from Aβ induced neurotoxicity. To further explore the neuroprotection of tan IIA, here we study the effects of tan IIA on the ER stress response in oligomeric Aβ1-42 (oAβ1-42)-induced SH-SY5Y cell injury. Our data showed that tan IIA pretreatment could increase cell viability and inhibit apoptosis caused by oAβ1-42. Furthermore, tan IIA markedly suppressed ER dilation and prevented oAβ1-42-induced abnormal expression of glucose regulated protein 78 (GRP78), initiation factor 2α (eIF2α), activating transcription factor 6 (ATF6), as well as inhibited the activation of C/EBP homologous protein (CHOP) and c-Jun N-terminal kinase (JNK) pathways. Moreover, tan IIA ameliorated oAβ1-42-induced Bcl-2/Bax ratio reduction, prevented cytochrome c translocation into cytosol from mitochondria, reduced oAβ1-42-induced cleavage of caspase-9 and caspase-3, suppressed caspase-3/7 activity, and increased mitochondrial membrane potential (MMP) and ATP content. Meanwhile, oAβ1-42-induced cell apoptosis and activation of ER stress can also be attenuated by the inhibitor of ER stress 4-phenylbutyric acid (4-PBA). Taken together, these data indicated that tan IIA protects SH-SY5Y cells against oAβ1-42-induced apoptosis through attenuating ER stress, modulating CHOP and JNK pathways, decreasing the expression of cytochrome c, cleaved caspase-9 and cleaved caspase-3, as well as increasing the ratio of Bcl-2/Bax, MMP and ATP content. Our results strongly suggested that tan IIA may be effective in treating AD associated with ER stress.
- Published
- 2019