21 results on '"Wanfu Hu"'
Search Results
2. Japanese Spotted Fever in Eastern China, 2013
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Jiabin Li, Wen Hu, Ting Wu, Hong-Bin Li, Wanfu Hu, Yong Sun, Zhen Chen, Yonglin Shi, Jia Zong, Adams Latif, Linding Wang, Li Yu, Xue-Jie Yu, Yan Liu, and Bo-Yu Liu
- Subjects
Rickettsia japonica ,Japanese spotted fever ,Haemaphysalis longicornis ticks ,rash ,fever ,China ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We isolated Rickettsia japonica from a febrile patient in Lu’an City, China, in 2013. Subsequently, we found an R. japonica seroprevalence of 54.8% (494/902) in the rural population of Anhui Province and an R. japonica prevalence in Haemaphysalis longicornis ticks of 0.5% (5/935). R. japonica and its tick vector exist in China.
- Published
- 2018
- Full Text
- View/download PDF
3. Influenza vaccine induces intracellular immune memory of human NK cells.
- Author
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Yaling Dou, Binqing Fu, Rui Sun, Wenting Li, Wanfu Hu, Zhigang Tian, and Haiming Wei
- Subjects
Medicine ,Science - Abstract
Influenza vaccines elicit antigen-specific antibodies and immune memory to protect humans from infection with drift variants. However, what supports or limits vaccine efficacy and duration is unclear. Here, we vaccinated healthy volunteers with annual vaccine formulations and investigated the dynamics of T cell, natural killer (NK) cell and antibody responses upon restimulation with heterologous or homologous influenza virus strains. Influenza vaccines induced potential memory NK cells with increased antigen-specific recall IFN-γ responses during the first 6 months. In the absence of significant changes in other NK cell markers (CD45RO, NKp44, CXCR6, CD57, NKG2C, CCR7, CD62L and CD27), influenza vaccines induced memory NK cells with the distinct feature of intracellular NKp46 expression. Indeed, surface NKp46 was internalized, and the dynamic increase in NKp46(intracellular)+CD56dim NK cells positively correlated with increased IFN-γ production to influenza virus restimulation after vaccination. In addition, anti-NKp46 antibodies blocked IFN-γ responses. These findings provide insights into a novel mechanism underlying vaccine-induced immunity and NK-related diseases, which may help to design persisting and universal vaccines in the future.
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- 2015
- Full Text
- View/download PDF
4. Inhibiting microRNA-424 in bone marrow mesenchymal stem cells-derived exosomes suppresses tumor growth in colorectal cancer by upregulating TGFBR3
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Li Ling, Zihui Li, Jun Luo, Wanfu Hu, Tao Ye, Wang Xinxin, Jiahua Tan, and Ning Zhang
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Adult ,Male ,0301 basic medicine ,Colorectal cancer ,Biophysics ,Down-Regulation ,Mice, Nude ,Vimentin ,Exosomes ,Biochemistry ,03 medical and health sciences ,Downregulation and upregulation ,Cell Movement ,Cell Line, Tumor ,microRNA ,medicine ,Animals ,Humans ,RNA, Small Interfering ,Molecular Biology ,Aged ,Cell Proliferation ,Aged, 80 and over ,Drug Carriers ,Mice, Inbred BALB C ,030102 biochemistry & molecular biology ,biology ,Mesenchymal Stem Cells ,Middle Aged ,Cell cycle ,medicine.disease ,G1 Phase Cell Cycle Checkpoints ,digestive system diseases ,Microvesicles ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Apoptosis ,biology.protein ,Cancer research ,Female ,Proteoglycans ,Colorectal Neoplasms ,Receptors, Transforming Growth Factor beta ,Transforming growth factor - Abstract
Objective MicroRNAs (miRNAs) have been demonstrated to be differently expressed in colorectal cancer (CRC) and were identified as biomarkers and therapeutic targets for CRC. We aimed to identify the effect of microRNA-424 (miR-424) on process of CRC. Methods Exosomes were obtained from bone marrow mesenchymal stem cells (BMSCs). MiR-424, transforming growth factor-β receptor 3 (TGFBR3) vimentin, S100A4, p-Smad1 expression in tissues and cells was measured. After treated with miR-424 inhibitor or TGFBR3 overexpression plasmid, the migration, invasion, cell cycle distribution and apoptosis of Lovo cells and exosomes-transfected Lovo cells were determined. The subcutaneous tumor models were established and the tumor growth was observed. The target relation between miR-424 and TGFBR3 was confirmed. Results MiR-424 was upregulated while TGFBR3 was downregulated in CRC tissues. TGFBR3 was targeted by miR-424. Inhibited miR-424 or elevated TGFBR3 upregulated p-Smad1, indicating that TGFBR3 mediated the Smad1 pathway, thus regulating CRC progression. MiR-424 inhibition or TGFBR3 restoration also suppressed migration and invasion of CRC cells, arrested the CRC cells at G0/G1 phase, and promoted CRC cell apoptosis. Moreover, exosomal miR-424 from BMSCs promoted CRC development. Conclusion Inhibited exosomal miR-424 from BMSCs inhibited malignant behaviors of CRC cells by targeting TGFBR3, thus suppressing the progression of CRC.
- Published
- 2021
5. Japanese Spotted Fever in Eastern China, 2013
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Linding Wang, Wanfu Hu, Wen Hu, Yan Liu, Yong Sun, Adams Latif, Bo-Yu Liu, Ting Wu, Hongbin Li, Li Yu, Zhen Chen, Yonglin Shi, Xue Jie Yu, Jiabin Li, and Jia Zong
- Subjects
Male ,Anhui Province ,Veterinary medicine ,Epidemiology ,urinalysis ,vector-borne infections ,lcsh:Medicine ,rash ,Haemaphysalis longicornis ticks ,Japonica ,spotted fever group Rickettsiae ,0302 clinical medicine ,Seroepidemiologic Studies ,030212 general & internal medicine ,Rickettsia ,Japanese Spotted Fever in Eastern China, 2013 ,bacteria ,Phylogeny ,fever ,seroprevalence ,Rickettsia japonica ,Eastern china ,tickborne infection ,17-kDa protein ,Middle Aged ,Tick vector ,Rash ,Infectious Diseases ,Japanese spotted fever ,medicine.symptom ,Haemaphysalis longicornis ,Microbiology (medical) ,China ,Ixodidae ,030231 tropical medicine ,Biology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,Research Letter ,medicine ,Animals ,Humans ,Seroprevalence ,lcsh:RC109-216 ,16S rRNA ,Tick Bites ,electron microscopy ,phylogenetic analysis ,Shandong Province ,lcsh:R ,blood chemistries ,Spotted Fever Group Rickettsiosis ,biology.organism_classification ,medicine.disease ,papular rash ,Arachnid Vectors - Abstract
We isolated Rickettsia japonica from a febrile patient in Lu’an City, China, in 2013. Subsequently, we found an R. japonica seroprevalence of 54.8% (494/902) in the rural population of Anhui Province and an R. japonica prevalence in Haemaphysalis longicornis ticks of 0.5% (5/935). R. japonica and its tick vector exist in China.
- Published
- 2018
6. In Situ Modification of Activated Carbon Made from Camellia oleifera Shell with Na2EDTA for Enhanced La3+ Recovery
- Author
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Lijinhong Huang, Xiangrong Zeng, Chunyan Fan, Lihong Liu, Shafiq Alam, Bin Zeng, Shaomin Liu, Wanfu Huang, and Ronghua Shu
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lanthanum ,Camellia oleifera shell ,in situ modification ,adsorption ,rare earth ,Mineralogy ,QE351-399.2 - Abstract
It is important to recover La3+ from metallurgical solutions or wastewater. However, the recovery rate of La3+ is usually less than 1% and the recovery methods are not environmentally friendly or user-friendly. Therefore, a straightforward, efficient, clean, and economically friendly method is needed. In this investigation, a modified adsorbent, COSAC-Na2EDTA-15, which was made from the Camellia oleifera shell (COS) and disodium ethylenediaminetetraacetic acid (Na2EDTA), was invented. In addition, characterization of the COSAC-Na2EDTA-15 adsorbent was conducted using SEM and XPS, and the principle of adsorption was revealed. The adsorption kinetics followed P-S-O KM, while the isotherm of COS-activated carbon (COSAC) aligned more closely with the Langmuir model. Compared to COSAC, the maximum La3+ adsorption capacity of COSAC-Na2EDTA-15 increased from 50 to 162.43 mg/g, and the content of O and N changed from 7.31% and 1.48% to 12.64% and 4.15%, respectively. The surface of the COSAC-Na2EDTA-15 exhibited abundant C, N, and O elements, and La3+ was detected on the sample surface after adsorption. The test and analysis results fully indicate that La3+ can be successfully adsorbed on the surface of COSAC-Na2EDTA-15. Because of its easy preparation, low cost, and superior performance, activated carbon made from COS finds extensive applications in the adsorption and recovery of rare earth elements.
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- 2024
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7. Human Infection with a Novel Avian-Origin Influenza A (H7N9) Virus
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Jun Han, Xuewei Xu, Jian Chen, Dexin Li, Bin Cao, Yu Wang, Junfeng Guo, Lei Yang, Yuelong Shu, Qun Li, Rongbao Gao, Yi Yang, Wenfei Zhu, Zhancheng Gao, Jie Dong, Xiang Zhao, Ke Xu, Zebao He, Haibo Qiu, Shiwen Wang, Zijian Feng, Shumei Zou, Dayan Wang, Yinzhong Shen, Y. Gu, Libo Dong, Tian Bai, Yunwen Hu, Weizhong Yang, Ye Zhang, Zhiyong Zhang, Wanfu Hu, Guizhen Wu, Xiyan Li, Lei Zhou, George F. Gao, Yun Zhu, Xiaodan Li, Nijuan Xiang, Zhenghong Yuan, Hongjie Yu, Yanping Zhang, Pei Hao, Zhijun Jie, and Hongzhou Lu
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Adult ,Male ,China ,Sequence analysis ,viruses ,Genome, Viral ,Biology ,Real-Time Polymerase Chain Reaction ,medicine.disease_cause ,H5N1 genetic structure ,Poultry ,Virus ,Avian Influenza A Virus ,Fatal Outcome ,Influenza, Human ,Reassortant Viruses ,medicine ,Animals ,Humans ,Gene ,Phylogeny ,Aged, 80 and over ,Respiratory Distress Syndrome ,Respiratory tract infections ,Reverse Transcriptase Polymerase Chain Reaction ,Sequence Analysis, DNA ,General Medicine ,Virology ,Influenza A virus subtype H5N1 ,Influenza A virus ,Influenza in Birds ,Female - Abstract
Infection of poultry with influenza A subtype H7 viruses occurs worldwide, but the introduction of this subtype to humans in Asia has not been observed previously. In March 2013, three urban residents of Shanghai or Anhui, China, presented with rapidly progressing lower respiratory tract infections and were found to be infected with a novel reassortant avian-origin influenza A (H7N9) virus.We obtained and analyzed clinical, epidemiologic, and virologic data from these patients. Respiratory specimens were tested for influenza and other respiratory viruses by means of real-time reverse-transcriptase-polymerase-chain-reaction assays, viral culturing, and sequence analyses.A novel reassortant avian-origin influenza A (H7N9) virus was isolated from respiratory specimens obtained from all three patients and was identified as H7N9. Sequencing analyses revealed that all the genes from these three viruses were of avian origin, with six internal genes from avian influenza A (H9N2) viruses. Substitution Q226L (H3 numbering) at the 210-loop in the hemagglutinin (HA) gene was found in the A/Anhui/1/2013 and A/Shanghai/2/2013 virus but not in the A/Shanghai/1/2013 virus. A T160A mutation was identified at the 150-loop in the HA gene of all three viruses. A deletion of five amino acids in the neuraminidase (NA) stalk region was found in all three viruses. All three patients presented with fever, cough, and dyspnea. Two of the patients had a history of recent exposure to poultry. Chest radiography revealed diffuse opacities and consolidation. Complications included acute respiratory distress syndrome and multiorgan failure. All three patients died.Novel reassortant H7N9 viruses were associated with severe and fatal respiratory disease in three patients. (Funded by the National Basic Research Program of China and others.).
- Published
- 2013
8. [Phylogenetic Analysis of the VP1 Region of Coxsackievirus A16 Strains Isolated in Anhui Province, 2014]
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Yonglin, Shi, Xian, Wang, Guoping, Chen, Jin, Zhang, and Wanfu, Hu
- Subjects
China ,Viral Proteins ,Genotype ,Molecular Sequence Data ,Enterovirus Infections ,Humans ,Phylogeny ,Enterovirus A, Human - Abstract
To study on the phylogenetic characterization of the VP1 genes of coxsackievirus A16 (CVA16) causing hand-food-mouth disease (HFMD) isolated from Anhui province in 2014. A total of 413 throat swab specimens from HFMD patients were collected during January to November, 2014 for the isolation and identification of enteroviruses using real-time RT-PCR assays. The VP1 regions of CVA16 isolates were amplified using RT-PCR and sequenced. And the phylogenetic tree was constructed among the VP1 regions of those isolates, the different genotypes and sub-genotypes of CVA16 strains. A total of 97 enteroviruses were isolated from 413 samples, the positive rate was 23.49% (97/413), including seventeen CVA16, seventy six HEV71 and four other enteroviruses. The results of the phylogenetic tree showed that 17.CVA16 strains isolated from Anhui in 2014 clustered within B1b evolution branch of B1 genotype. The nucleotide and amino acid sequence identities were 95.30%-100% and 98.70%-100% among the isolates, respectively, but within B1b branch of 17 strains formed several small transmission chains. The nucleotide acid of 17 CVA16 isolates in Anhui province were closed to the strains isolated from Yunnan, Hunan, Guangdong, Tibet and Jiangsu, especially from Hunan in 2013 and from Shenzhen of Guangdong in 2014, the identity were 96.40%-99.70%. The CVA16 strains isolated from Anhui in 2014 were all belong to genetic subtype B1b of B1 genotype was dominant, and among those isolates, several small virus transmission chains had formed with co-circulating and evolution.
- Published
- 2016
9. Person-to-Person Transmission of Severe Fever with Thrombocytopenia Syndrome Virus
- Author
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Yubi Wang, Yu Wang, Jun Ren, Jiabin Wu, Ling Mei, David H. Walker, Xue Jie Yu, Qun Li, Yan Liu, and Wanfu Hu
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Adult ,Male ,Phlebovirus ,China ,medicine.medical_specialty ,Antibodies, Viral ,Bunyaviridae Infections ,Polymerase Chain Reaction ,Microbiology ,Risk Factors ,Virology ,Internal medicine ,Cluster Analysis ,Humans ,Medicine ,Seroconversion ,Fluorescent Antibody Technique, Indirect ,Retrospective Studies ,Leukopenia ,biology ,business.industry ,Transmission (medicine) ,Antibody titer ,SFTS virus ,Middle Aged ,biology.organism_classification ,medicine.disease ,Severe fever with thrombocytopenia syndrome ,Infectious Diseases ,Immunology ,Female ,medicine.symptom ,Databases, Nucleic Acid ,business ,Severe fever with thrombocytopenia syndrome virus - Abstract
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a newly discovered bunyavirus, SFTS virus (SFTSV), and causes high fatality (12% on average and as high as 30%). The objective of this study was to determine whether SFTSV could be transmitted from person to person. We analyzed sera of 13 patients from two clusters of unknown infectious diseases that occurred between September and November of 2006 in Anhui Province of China for SFTSV antibody by indirect immunofluorescence assay and for SFTSV RNA by RT-PCR. We found that all patients (n=14) had typical clinical symptoms of SFTS including fever, thrombocytopenia, and leukopenia and all secondary patients in both clusters got sick at 6-13 days after contacting or exposing to blood of index patients. We demonstrated that all patients in cluster 1 including the index patient and nine secondary patients and all three secondary patients in cluster 2 had seroconversion or fourfold increases in antibody titer to SFTSV and/or by RT-PCR amplification of SFTSV RNA from the acute serum. The index patient in cluster 2 was not analyzed because of lack of serum. No person who contacted the index patient during the same period, but were not exposed to the index patient blood, had got illness. We concluded that SFTSV can be transmitted from person to person through contacting patient's blood.
- Published
- 2012
10. Detection of Highly Pathogenic Avian Influenza Virus H5N1 in Peripheral Blood Mononuclear Cells and Stool of a Patient
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Ming-Ying Ma, Jun He, Wanfu Hu, Yan Liu, Dexin Li, Jianjun Wang, Yuelong Shu, Wei Wang, and Rongbao Gao
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Biochemistry (medical) ,Clinical Biochemistry ,Hemagglutinin ,Biology ,medicine.disease_cause ,Peripheral blood mononuclear cell ,Virology ,Virus ,Influenza A virus subtype H5N1 ,Reverse transcription polymerase chain reaction ,chemistry.chemical_compound ,chemistry ,Immunoglobulin M ,biology.protein ,medicine ,Fluorescein isothiocyanate ,Neuraminidase - Abstract
A fatal case caused by an H5N1 virus infection was investigated. In addition to serials of clinical chemistry assays, we tested the patient’s peripheral blood mononuclear cells (PBMC) and stool by reverse transcription polymerase chain reaction (RT-PCR), real-time RT-PCR, and/or immunofluorescent assay. Our results suggested that PBMC can carry the virus and help the H5N1 virus spread to the human gut, resulting in infection with inflammation and viral discharge. * PBMC : peripheral blood mononuclear cells RT-PCR : reverse transcription polymerase chain reaction ARDS : acute respiratory distress syndrome OB : occult blood IgM : immunoglobulin M H5 : hemagglutinin N1 : neuraminidase PCR : polymerase chain reaction WHO : World Health Organization PBS : phosphate buffered saline FITC : fluorescein isothiocyanate NP : nucleoprotein HPAI : highly pathogenic avian influenza
- Published
- 2011
11. [Study of molecular of 80 clinical streptococcus pneumoniae strains in Maanshan area]
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Daoli, Chen, Machao, Li, Haijian, Zhou, Guojun, Liu, Yan, Wang, Baiqi, Yu, Mingmei, Shi, Xianfeng, Cheng, Ying, Hong, Jin, Chen, Wanfu, Hu, Jun, Ren, and Shengwei, Zhan
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China ,Streptococcus pneumoniae ,Humans - Published
- 2015
12. Investigation of Hydrated Dy(III) and MgSO4 Leaching Agent Ion Adsorption on (001) Surface of Montmorillonite: A Study Using Density Functional Theory
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Lijinhong Huang, Zhiqiang Zou, Shaomin Liu, Lihong Liu, Wengang Xiao, Yantao Qian, Shafiq Alam, and Wanfu Huang
- Subjects
DFT ,Dy(III) ,MgSO4 ,SO42− ,kaolinite crystalline ,adsorption ,Mineralogy ,QE351-399.2 - Abstract
Kaolinite is one of the principal rare earth element (REE) ion-adsorption clays that hosts a wide range of elements, including Dy(III) as a representative example. Ammonium sulfate is a typical salt used to leach REEs. Due to the carbon dioxide emissions which occur during ammonia production, it is urgently necessary to develop low environmental pollution leaching agents that can replace (NH4)2SO4. MgSO4 is regarded as the most promising eco-friendly leaching agent. Herein, the first-principles plane-wave pseudopotential method based on the density functional theory (DFT) was used to investigate the stable adsorption structures of Dy(III) and its hydrated ions, MgSO4 leaching agent ions and the corresponding hydrated ions on the surface of kaolinite, which revealed the adsorption mechanism of Dy(III), Mg(II), and SO42− on the silico–oxygen plane and the aluminum–hydroxyl plane of kaolinite. Based on the research results of the steric hindrance effect of Dy(III) on the silico–oxygen plane and the aluminum–hydroxyl plane of kaolinite, the adsorption of Dy(H2O)103+ was more stable on the silico–oxygen plane. It was easier to leach out Dy(III) with MgSO4, while SO42− tended to interact with the rare earth ions in an aqueous solution. The results provide theoretical guidance for efficient rare earth extraction and obtaining novel efficient leaching agents.
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- 2023
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13. O:8 serotype Yersinia enterocolitica strains in China
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Haiyan Qiu, Jianguo Xu, Zhigang Cui, Huaiqi Jing, Jinchuan Yang, Dong Jin, Yuchun Xiao, Wanfu Hu, Hua Wang, Xianjun Wang, Yanmei Xu, Biao Kan, Shengli Xia, and Xin Wang
- Subjects
Serotype ,DNA, Bacterial ,China ,Yersinia Infections ,Virulence Factors ,Virulence ,Microbiology ,Mice ,Random Allocation ,Pulsed-field gel electrophoresis ,Tumor Cells, Cultured ,Animals ,Humans ,Serotyping ,Yersinia enterocolitica ,Gene ,biology ,Strain (chemistry) ,General Medicine ,biology.organism_classification ,Enterobacteriaceae ,Virology ,Electrophoresis, Gel, Pulsed-Field ,Bacterial Vaccines ,Food Microbiology ,Bacteria ,Food Science - Abstract
Serotypes O:3, O:8 and O:9 Yersinia enterocolitica strains carrying virulence determinants are common pathogens causing human infections. In many years of surveillance in China for Y. enterocolitica, no pathogenic O:8 strains have been found where the isolated O:8 serotypes lacked the major virulence genes and in contrast to O:3 and O:9 strains, none of the O:8 isolates were from humans. These O:8 isolates lack ail, ystA, yadA and virF genes but possess the ystB gene and all belong to Biotype 1A. These O:8 strains did not kill mice and could protect immunized mice against challenge with a pathogenic O:8 strain. Compared to the Chinese pathogenic O:3 and O:9 strains which have similar pulsed-field gel electrophoresis patterns, the 39 Chinese O:8 animal and food isolates were different from the pathogenic O:8 reference strains. This suggests the O:8 strains lacking virulence determinants may not disseminate rapidly in humans and are maintained in animal reservoirs; and therefore exhibit higher variance and divergence from the virulent type.
- Published
- 2007
14. Influenza Vaccine Induces Intracellular Immune Memory of Human NK Cells
- Author
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Haiming Wei, Binqing Fu, Yaling Dou, Wenting Li, Zhigang Tian, Wanfu Hu, and Rui Sun
- Subjects
Adult ,Male ,Influenza vaccine ,T cell ,lcsh:Medicine ,medicine.disease_cause ,Interferon-gamma ,Antigen ,Immunity ,medicine ,Influenza A virus ,Humans ,lcsh:Science ,Multidisciplinary ,biology ,lcsh:R ,Middle Aged ,Vaccine efficacy ,Antigens, Differentiation ,Virology ,Killer Cells, Natural ,Vaccination ,medicine.anatomical_structure ,Influenza Vaccines ,Immunology ,biology.protein ,Female ,lcsh:Q ,Antibody ,Immunologic Memory ,Research Article - Abstract
Influenza vaccines elicit antigen-specific antibodies and immune memory to protect humans from infection with drift variants. However, what supports or limits vaccine efficacy and duration is unclear. Here, we vaccinated healthy volunteers with annual vaccine formulations and investigated the dynamics of T cell, natural killer (NK) cell and antibody responses upon restimulation with heterologous or homologous influenza virus strains. Influenza vaccines induced potential memory NK cells with increased antigen-specific recall IFN-γ responses during the first 6 months. In the absence of significant changes in other NK cell markers (CD45RO, NKp44, CXCR6, CD57, NKG2C, CCR7, CD62L and CD27), influenza vaccines induced memory NK cells with the distinct feature of intracellular NKp46 expression. Indeed, surface NKp46 was internalized, and the dynamic increase in NKp46(intracellular)+CD56dim NK cells positively correlated with increased IFN-γ production to influenza virus restimulation after vaccination. In addition, anti-NKp46 antibodies blocked IFN-γ responses. These findings provide insights into a novel mechanism underlying vaccine-induced immunity and NK-related diseases, which may help to design persisting and universal vaccines in the future.
- Published
- 2015
15. Synthesis of Micro-Electrolysis Composite Materials from Blast Furnace Dust and Application into Organic Pollutant Degradation
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Xiangrong Zeng, Ting Xie, Bin Zeng, Lijinhong Huang, Xindong Li, and Wanfu Huang
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blast furnace dust ,micro-electrolysis ,organic pollutants ,degradation ,Chemistry ,QD1-999 - Abstract
A micro-electrolysis material (MEM) was successfully prepared from carbothermal reduction of blast furnace dust (BFD) and coke as raw materials in a nitrogen atmosphere. The MEM prepared from BFD had strong ability in removing methyl orange, methylene blue, and rose bengal (the removal rates of methyl orange and methylene blue were close to 100%). X-ray diffraction showed that the iron mineral in BFD was ferric oxide, which was converted to zero-valent iron after being reduced by calcination. Scanning electron microscopy showed that nano-scale zero-valent iron particles were formed in the MEM. In short, the MEM prepared from BFD can effectively degrade organic pollutants.
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- 2022
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16. Adsorption of Hydrated Pr3+ and NH4+/Mg2+ Ions onto the (001) Surface of Montmorillonite: A DFT Analysis with Experimental Verification
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Zhiqiang Zou, Lijinhong Huang, Xindong Li, Jie Xu, Xiangrong Zeng, Ronghua Shu, Bin Xiao, Jiacai Ou, and Wanfu Huang
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DFT ,Pr3+ ,Mg2+ ,NH4+ ,montmorillonite ,adsorption ,Mineralogy ,QE351-399.2 - Abstract
Montmorillonite is a major mineral present in ion-adsorption rare earth ores, and the microscopic adsorption states of rare earth ions on its surface are of a great significance for the efficient exploitation of ion-adsorption rare earth ores. In this article, density functional theory calculations were used to investigate the adsorption mechanisms and bonding characteristics of hydrated Pr, Mg and NH4 ions on the (001) surface of montmorillonite. Pr3+ exhibited a directed tendency geometry with Pr(H2O)103+, which was adsorbed onto montmorillonite by hydrogen bonding with an adsorption energy of −1182 kJ/mol, and one coordinated H2O ligand was separated from the first hydration layer of Pr. Both hydrated Mg and NH4 ions were adsorbed onto the montmorillonite surface through hydrogen bonds, and the adsorption energies were −206 and −188 kJ/mol, respectively, indicating that the adsorption stability of the hydrated Mg ion was slightly higher than that of the hydrated NH4 ion, but both were lower than that of hydrated Pr (−1182 kJ/mol). Hence, higher concentrations of Mg and NH4 ions than rare earth ions would be necessary in the leaching process of ion-adsorption rare earth ores. Additionally, desorption experiments revealed that the recovery of Pr3+ by Mg2+ with a concentration of 38 mmol/L is 80%, while it is only 65% with the same concentration of NH4+, and the concentrations of Mg2+ and NH4+ were much higher than that of Pr3+ in lixivium, which is consistent with the DFT calculations.
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- 2022
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17. Separation of Copper-Molybdenum Flotation Concentrate by Superconducting High-Gradient Magnetic Separation
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Zekai Wang, Xindong Li, Zhaolian Wang, Wanfu Huang, Guanfa Liu, Chaocong Zeng, and Lijinhong Huang
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superconductive magnetic separation ,chalcopyrite ,molybdenite ,separation process ,Mineralogy ,QE351-399.2 - Abstract
Separation of chalcopyrite from molybdenite is currently mainly carried out by flotation, but this process is costly because of the extensive use of inhibitors. This study briefly describes a 7.0T/100CGC low-temperature superconducting magnetic separator and discusses its separation principle as well as the effect of magnetic induction on chalcopyrite separation from molybdenite. A molybdenum (Mo) concentrate assaying 6.00% copper (Cu) and 19.01% Mo was magnetically sorted using a diamond-shaped steel rod medium mesh at a feed concentration of 20% and a pulp flow rate of 5 L/min from a Cu-Mo flotation concentrate with 88% of particles smaller than 23 μm using the separator. A Mo concentrate assaying 0.46% Cu and 16.28% Mo was finally obtained with a roughing (1.3 T)-cleaning (5 T) superconducting magnetic separation process. Similarly, the superconducting magnetic separator was performed to separate a Cu-Mo bulk flotation concentrate, and produced Cu concentrate assaying 19.64% Cu and 0.03% Mo from the bulk concentrate assaying 18.52% Cu and 0.39% Mo with a particle size of less than 0.074 mm. At a magnetic induction of 7 T, a pulp concentration of 20% and a feed velocity of 5 L/min, the grade and recovery of Cu in the magnetic product were 19.64% and 81.59%, respectively, whereas the grade and recovery of Mo in the non-magnetic product were 1.52% and 90.07%, respectively. Superconducting magnetic separation has potential applications for removing Cu from Mo concentrates, and separating Cu and Mo from Cu-Mo bulk flotation concentrates.
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- 2022
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18. Adsorption of Y(III) on the Interface of Kaolinite-H2O: A DFT Study
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Xiangrong Zeng, Bin Zeng, Lijinhong Huang, Liang Zhong, Xindong Li, and Wanfu Huang
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rare earth ,yttrium ,kaolinite-H2O ,adsorption ,density functional theory ,Mineralogy ,QE351-399.2 - Abstract
Ion-adsorbed rare earth minerals have rare earth ions adsorbed on the surfaces of clay minerals such as kaolinite and have high contents of medium and heavy rare earth elements. They are important resources supporting the development of high-tech industries. In this study, the CASTEP module in Materials Studio was used to study the adsorption of the rare earth ion Y(III) on the interface of (Al-OH)-H2O and (Si-O)-H2O with density functional theory. The monitoring and calculation of the chemical bond of the adsorption structure showed that Y(III) on the (Al-OH)-H2O interface has a bond with O32, O34, and water molecules in the interface. In the (Si-O)-H2O interface, Y(III) interacts with O3, O4, and O10 to form new chemical bonds. The Mulliken population and density of states analysis showed that Y(III) bonds with surface oxygen atoms and water molecules in the kaolinite-H2O interface, and finally adsorbs on the surface of kaolinite in the form of metal ion hydrate.
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- 2022
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19. Detection of Highly Pathogenic Avian Influenza Virus H5N1 in Peripheral Blood Mononuclear Cells and Stool of a Patient.
- Author
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Yan Liu, Rongbao Gao, Wanfu Hu, Mingying Ma, Wei Wang, Jun He, Jianjun Wang, Dexin Li, and Yuelong Shu
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AVIAN influenza diagnosis ,FECAL analysis ,AVIAN influenza ,BIOPHYSICS ,BLOOD cell count ,BLOOD cells ,CLINICAL chemistry ,FLUORESCENT antibody technique ,RESEARCH methodology ,POLYMERASE chain reaction ,RESEARCH funding ,URINALYSIS ,VIRAL load ,REVERSE transcriptase polymerase chain reaction ,INFLUENZA A virus, H5N1 subtype ,INFECTIOUS disease transmission - Abstract
A fatal case caused by an H5N1 virus infection was investigated. In addition to serials of clinical chemistry assays, we tested the patient's peripheral blood mononuclear cells (PBMC) and stool by reverse transcription polymerase chain reaction (RT-PCR), real-time RT-PCR, and/or immunofluorescent assay. Our results suggested that PBMC can carry the virus and help the H5N1 virus spread to the human gut, resulting in infection with inflammation and viral discharge. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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20. The settling behavior of quartz using chitosan as flocculant
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Bo Feng, Jinxiu Peng, Xianwen Zhu, and Wanfu Huang
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Quartz ,Flocculant ,Chitosan ,Settling ,pH ,Mining engineering. Metallurgy ,TN1-997 - Abstract
The settling behavior of quartz using chitosan as flocculant has been studied and the mechanism has been discussed. The sedimentation results show that pH has an influence on the settling behavior of quartz particles and the sedimentation velocity is more quickly at acidic pH range. Chitosan is a useful flocculant for the settling of quartz but its flocculation effect is influenced greatly by pH. The sedimentation velocity of quartz is quickly and the volume of sediment is large when chitosan was added at pH 9. The reason is that chitosan is only sparingly soluble in water at pH 9, thus the adsorption amount is large and produce strongly flocculation effect. However, when the pH was changed from 9 to 3, the adsorbed chitosan desorption from quartz surface and the flocculation effect disappeared. The flocs were disorganized to the particles and the sediment can be consolidated to significantly higher densities.
- Published
- 2017
- Full Text
- View/download PDF
21. Human Infection with a Novel Avian-Origin Influenza A (H7N9) Virus.
- Author
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Rongbao Gao, Bin Cao, Yunwen Hu, Zijian Feng, Dayan Wang, Wanfu Hu, Jian Chen, Zhijun Jie, Haibo Qiu, Ke Xu, Xuewei Xu, Hongzhou Lu, Wenfei Zhu, Zhancheng Gao, Nijuan Xiang, Yinzhong Shen, Zebao He, Yong Gu, Zhiyong Zhang, and Yi Yang
- Subjects
- *
H7N9 Influenza , *INFLUENZA A virus, H7N9 subtype , *AVIAN influenza A virus , *INFECTIOUS disease transmission - Abstract
The article discusses research on human infection with a novel avian-origin influenza A (H7N9) virus found in three residents of Shanghai or Anhui, China in March 2013. It references a study by Rongbao Gao et al., published in a 2013 issue of "The New England Journal of Medicine." Results of the study demonstrate the association of novel reassortant H7N9 viruses with severe and fatal respiratory disease in the patients.
- Published
- 2013
- Full Text
- View/download PDF
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