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Soil-based microorganisms assume a direct and crucial role in the promotion of soil health, quality and fertility, all factors known to contribute heavily to the quality and yield of agricultural products. Cover cropping, used in both traditional and organic farming, is a particularly efficient and environmentally favorable tool for manipulating microbiome composition in agricultural soils and has had clear benefits for soil quality and crop output. Several long-term investigations have evaluated the influence of multi-mix (multiple species) cover crop treatments on soil health and microbial diversity. The present study investigated the short-term effects of a seven species multi-mix cover crop treatment on soil nutrient content and microbial diversity, compared to a single-mix cover crop treatment and control. Analysis of 16S sequencing data of isolated soil DNA revealed that the single-mix cover crop treatment decreased overall microbial abundance and diversity, whereas the control and multi-mix treatments altered the overall microbial composition in similar fluctuating trends. Furthermore, we observed significant changes in specific bacteria belonging to the phyla Acidobacteria, Actinobacteria, Planctomycetes, Proteobacteria and Verrucombicrobia for all treatments, but only the single-mix significantly decreased in abundance of the selected bacteria over time. Our findings indicate that the control and multi-mix treatments are better at maintaining overall microbial composition and diversity compared to the single-mix. Further study is required to elucidate the specific difference between the treatment effect of the multi-mix treatment and the control, given that their microbial composition changes over time were similar but they diverge into two populations of unique bacterial types by the end of this short-term study. [ABSTRACT FROM AUTHOR]

With the wave of digitisation sweeping through the higher education sector, end-of-life outcomes for decommissioned computers will pose a serious sustainability challenge for higher education institutions. This challenge presents an opportunity for universities to implement and demonstrate best practice in sustainability; in this case, computer re-use schemes. The paper addresses the practical realisation of sustainability innovation in a social and institutional context via an examination of the first computer re-use scheme implemented within an Australian university. It also contributes a unique, institutional and social perspective to the study of a sustainability innovation, computer re-use schemes. The key factors that contributed to the successful implementation of the Scheme are derived and presented as a social and institutional model for implementing sustainability initiatives. A summary of the model is presented in the graphical abstract. Given the complex organisational structure of universities, these learnings can be relevant to a wide range of institutions and the implementation of other sustainability initiatives. Image 1 • A social and institutional model for sustainability innovation is presented. • Student leadership and initiative was the catalyst for the re-use scheme. • A common language and stated objective for sustainability and social impact supported the scheme. • Partnerships led to new organisational and delivery set-ups for sustainability innovation. [ABSTRACT FROM AUTHOR]

The availability of high-throughput genomic data has led to several challenges in recent genetic association studies, including the large number of genetic variants that must be considered and the computational complexity in statistical analyses. Tackling these problems with a marker-set study such as SNP-set analysis can be an efficient solution. To construct SNP-sets, we first propose a clustering algorithm, which employs Hamming distance to measure the similarity between strings of SNP genotypes and evaluates whether the given SNPs or SNP-sets should be clustered. A dendrogram can then be constructed based on such distance measure, and the number of clusters can be determined. With the resulting SNP-sets, we next develop an association test HDAT to examine susceptibility to the disease of interest. This proposed test assesses, based on Hamming distance, whether the similarity between a diseased and a normal individual differs from the similarity between two individuals of the same disease status. In our proposed methodology, only genotype information is needed. No inference of haplotypes is required, and SNPs under consideration do not need to locate in nearby regions. The proposed clustering algorithm and association test are illustrated with applications and simulation studies. As compared with other existing methods, the clustering algorithm is faster and better at identifying sets containing SNPs exerting a similar effect. In addition, the simulation studies demonstrated that the proposed test works well for SNP-sets containing a large proportion of neutral SNPs. Furthermore, employing the clustering algorithm before testing a large set of data improves the knowledge in confining the genetic regions for susceptible genetic markers. [ABSTRACT FROM AUTHOR]

X-chromosome dosage compensation by the MSL (male-specific lethal) complex is required in Drosophila melanogaster to increase gene expression from the single male X to equal that of both female X chromosomes. Instead of focusing solely on protein complexes released from DNA, here we used chromatin-interacting protein MS (ChIP-MS) to identify MSL interactions on cross-linked chromatin. We identified MSL-enriched histone modifications, including histone H4 Lys16 acetylation and histone H3 Lys36 methylation, and CG4747, a putative Lys36-trimethylated histone H3 (H3K36me3)-binding protein. CG4747 is associated with the bodies of active genes, coincident with H3K36me3, and is mislocalized in the Set2 mutant lacking H3K36me3. CG4747 loss of function in vivo results in partial mislocalization of the MSL complex to autosomes, and RNA interference experiments confirm that CG4747 and Set2 function together to facilitate targeting of the MSL complex to active genes, validating the ChIP-MS approach. [ABSTRACT FROM AUTHOR]

Long noncoding RNAs (lncRNAs) have important regulatory roles and can function at the level of chromatin. To determine where lncRNAs bind to chromatin, we developed capture hybridization analysis of RNA targets (CHART), a hybridization-based technique that specifically enriches endogenous RNAs along with their targets from reversibly cross-linked chromatin extracts. CHART was used to enrich the DNA and protein targets of endogenous lncRNAs from flies and humans. This analysis was extended to genome-wide mapping of roX2, a well-studied ncRNA involved in dosage compensation in Drosophila. CHART revealed that roX2 binds at specific genomic sites that coincide with the binding sites of proteins from the male-specific lethal complex that affects dosage compensation. These results reveal the genomic targets of roX2 and demonstrate how CHART can be used to study RNAs in a manner analogous to chromatin immunoprecipitation for proteins. [ABSTRACT FROM AUTHOR]

Abstract: Due to new technologies, potential variables are collected in a more efficient manner than before. Thus, how to sift effective variable from an abundance of newly discovered markers is an important issue. To this end, an adaptive sequential method is proposed for estimating the diagnostic power based on the partial area under the receiving operating characteristic curve of variables. However, the estimating accuracy of these performance criteria are usually affected by the sample sizes of two groups, say case and control. Thus, we need to decide which group to sample from as that in a two-armed bandit problem. Sequential confidence interval estimation methods are used to control the accuracy of the estimates. Both two-sided fixed-width and one-sided confidence intervals are considered. The proposed methods are proved to be optimal, since they not only guarantee the accuracy of the performance estimates, but also maintain the ratio of cases to controls converging to their corresponding optima in terms of minimization of the variance of the estimate. When the availabilities of two classes are imbalanced or the costs of sampling them are seriously unequal, our method can include suchlike information as a part of its criteria. [Copyright &y& Elsevier]

Abstract: Parkinson’s disease (PD) is a common neurodegenerative disease of unknown etiology. Evidence suggests a role for protein misfolding in disease pathogenesis. One pathologic feature observed in dopaminergic neurons is the intracytoplasmic eosinophilic inclusions known as Lewy bodies. One component of Lewy bodies, the presynaptic protein, α-synuclein forms oligomers and higher order aggregates and is proposed to be involved in dopaminergic neuronal death. In an effort to discriminate between α-synuclein conformational forms as well as design potential disruptors of pathogenic misfolding we panned a human phage antibody library for anti-synuclein single chain antibodies (scFvs). We identified six scFvs which recognize different conformers of α-synuclein in both an ELISA and Western blot analysis. These scFvs may further our understanding of α-synuclein’s role in PD. [Copyright &y& Elsevier]

• We conducted a lab-in-the-field experiment in rural Yunnan Province, China. • The experiment included a public goods game with punishment and a trust game. • Group composition and ethnicity information were controlled experimentally. • Subjects are less willing to punish in-group members in presence of out-group members. • Anticipation of this response leads to lower contributions in mixed groups. Do people discriminate between co-ethnics and others in cooperative interactions? In an experiment in China, we find that participants in trust games send around 15% more to partners they know to be co-ethnics than to those whose ethnicity they do not know. Receivers' behavior is determined by amounts received and not by perceived ethnicity. In line with previous literature we find that subjects contribute more to public goods in ethnically homogeneous groups than in mixed groups. We find evidence for a new explanation that is not due to different intrinsic preferences for cooperation with ingroup and outgroup members. Instead, subjects' willingness to punish in-group members for free-riding is reduced when out-group members are present. This leads to lower contributions and net earnings in mixed groups. Thus favoritism towards co-ethnics can hurt both those engaging in favoritism and those being favored. [ABSTRACT FROM AUTHOR]

The article presents a case study of a 59-year-old right-handed woman presented with an encephalopathy and her family reported a two-week history of worsening cognition and confusion, with two months' history of deteriorating gait. It revealed the characteristic magnetic resonance findings of linear perivascular enhancement.

Provides news briefs on science and medicine research as of April 2002. Association between non-Hodgkin lymphoma and polyomavirus SV40; Relativity between immune system and arthritis.

Presents research news related to the field of medicine as of October 2001. Development of an HIV vaccine that elicits potent cell-mediated immune response in controlling AIDS; Eradication of cancer cells using a single-stranded DNA virus called adeno-associated virus.

Reports findings of a study on the process of neurogenesis, which is recognized to be an ongoing process in adult mammalian brain. Growth of neural stem cells in specialized regions of the adult brain such as the subventricular zone (SVZ); Reference to a report published in the December 2000 issue of the journal 'Neuron,' vol. 28, 713-726; Demonstration of the expression of Noggin SVZ.

[Display omitted] • Tropical cyclones cause torrential rainfall, severe property damage, and loss of life. • Probabilistic forecast of tropical cyclone rainfall provides uncertainty evaluation. • The Bayesian mixture model combines ensemble member forecasts and historical data. • Each grid has a specific Bayesian model and all grids are predicted simultaneously. • The model provides flexibility in weighting information from different sources. Statistical post-processing has been widely adopted in probabilistic forecasting, especially when a predictive distribution of weather variables is of interest. Among the available post-processing methods, a common tool is the ensemble Bayesian model averaging. Challenges arise when it is applied to heavy rain prediction, including the precipitation produced by tropical cyclones. The issues are related to the rare extreme event and limited available data for model training. To address this, we propose in this research a Bayesian mixture model, Bmix, based on information from forecast members and historical tropical cyclones, rather than solely on immediately observed precipitation data. This approach provides a grid-specific Bayesian predictive distribution and an easy-to-derive probabilistic forecast with posterior samples generated from the Markov chain Monte Carlo algorithm. This post-processing procedure can be executed on each grid simultaneously, reducing the computational time significantly. In addition, a categorized Bmix (C.Bmix) is implemented to accommodate different scenarios such as heavy or torrential rain in applications. The proposed approaches are demonstrated on the 24-hour accumulated precipitation forecast of a category 4-equivalent super typhoon Dujuan landing in Taiwan in 2015; while two others, Matmo and Soudelor, are adopted to provide historical information. Over the Taiwan main island, a total of 1282 grids are considered, each with a resolution of 5 km and 20 ensemble member forecasts from the Weather Research and Forecasting Ensemble Prediction System (WEPS) at a 6-hour interval during the typhoon period. The analyses show that the proposed mixture approaches present larger percentages of positive CRPSS values than traditional BMA. For instance, at the time when Dujuan made landfall, the percentages are 92.2 % for Bmix, 95.2 % for C.Bmix, and 15.4 % for BMA when the true precipitation amount exceeds 200 mm/24 h. The proposed methods outperform in the probability prediction and the overall pattern of precipitation. [ABSTRACT FROM AUTHOR]

Both parents and offspring have evolved mating preferences that enable them to select mates and children‐in‐law to maximize their inclusive fitness. The theory of parent–offspring conflict predicts that preferences for potential mates may differ between parents and offspring: individuals are expected to value biological quality more in their own mates than in their offspring's mates and to value investment potential more in their offspring's mates than in their own mates. We tested this hypothesis in China using a naturalistic ‘marriage market’ where parents actively search for marital partners for their offspring. Parents gather at a public park to advertise the characteristics of their adult children, looking for a potential son or daughter‐in‐law. We presented 589 parents and young adults from the city of Kunming (Yunnan, China) with hypothetical mating candidates varying in their levels of income (proxy for investment potential) and physical attractiveness (proxy for biological quality). We found some evidence of a parent–offspring conflict over mate choice, but only in the case of daughters, who evaluated physical attractiveness as more important than parents. We also found an effect of the mating candidate's sex, as physical attractiveness was deemed more valuable in a female potential mate by parents and offspring alike. [ABSTRACT FROM AUTHOR]

Chromatin plays a critical role in faithful implementation of gene expression programs. Different post-translational modifications (PTMs) of histone proteins reflect the underlying state of gene activity, and many chromatin proteins write, erase, bind, or are repelled by, these histone marks. One such protein is UpSET, the Drosophila homolog of yeast Set3 and mammalian KMT2E (MLL5). Here, we show that UpSET is necessary for the proper balance between active and repressed states. Using CRISPR/Cas-9 editing, we generated S2 cells that are mutant for upSET. We found that loss of UpSET is tolerated in S2 cells, but that heterochromatin is misregulated, as evidenced by a strong decrease in H3K9me2 levels assessed by bulk histone PTM quantification. To test whether this finding was consistent in the whole organism, we deleted the upSET coding sequence using CRISPR/Cas-9, which we found to be lethal in both sexes in flies. We were able to rescue this lethality using a tagged upSET transgene, and found that UpSET protein localizes to transcriptional start sites (TSS) of active genes throughout the genome. Misregulated heterochromatin is apparent by suppressed position effect variegation of the wm4 allele in heterozygous upSET-deleted flies. Using nascent-RNA sequencing in the upSET-mutant S2 lines, we show that this result applies to heterochromatin genes generally. Our findings support a critical role for UpSET in maintaining heterochromatin, perhaps by delimiting the active chromatin environment. [ABSTRACT FROM AUTHOR]

BACKGROUND: Oral care may decrease the development of ventilator-associated pneumonia (VAP) and improve oral hygiene. However, little evidence is available to guide the development of oral care protocols. The practical effect of toothbrushing on VAP development and oral health and hygiene improvement is inconclusive. PURPOSE: This study evaluated the effects in postneurosurgical, intensive care unit patients of brushing teeth twice daily with purified water on VAP rates and oral health or hygiene. METHODS: This study conducted a randomized controlled pilot trial. Patients consecutively admitted to the surgical intensive care unit at a suburban hospital in 2007 were invited to participate if they met two inclusion criteria: (a) under ventilator support for at least 48 to 72 hours and (b) no current pneumonia. Upon obtaining informed consent, subjects were randomized into experimental and control groups. Both groups received usual hospital care, that is, daily oral care using cotton swabs. The experimental group additionally received a twice-daily oral care protocol of toothbrushing with purified water, elevating the head of the bed, and before-and-after hypopharyngeal suctioning. The control group also received twice-daily mock oral care (elevating the head of the bed, moisturizing the lips, and before-and-after hypopharyngeal suctioning). VAP was defined by a clinical pulmonary infection score of > 6. Oral hygiene and health was assessed after conclusion of the intervention. RESULTS: Patients (N = 53) were predominantly male (64.2%), mean age was 60.6 years old, and most had received emergency surgery (75.5%). After 7 days of toothbrushing with purified water, cumulative VAP rates were significantly lower in the experimental (17%) than in the control (71%; p <.05) group. The experimental group also had significantly better scores for oral health (p <.05) and plaque index (p <.01). CONCLUSION/IMPLICATION FOR PRACTICE: Findings suggest that, as an inexpensive alternative to existing protocols, toothbrushing twice daily with purified water reduces VAP and improves oral health and hygiene. [ABSTRACT FROM AUTHOR]

Background: Oral care may decrease the development of ventilator-associated pneumonia (VAP) and improve oral hygiene. However, little evidence is available to guide the development of oral care protocols. The practical effect of toothbrushing on VAP development and oral health and hygiene improvement is inconclusive. Purpose: This study evaluated the effects in postneurosurgical, intensive care unit patients of brushing teeth twice daily with purified water on VAP rates and oral health or hygiene. Methods: This study conducted a randomized controlled pilot trial. Patients consecutively admitted to the surgical intensive care unit at a suburban hospital in 2007 were invited to participate if they met two inclusion criteria: (a) under ventilator support for at least 48 to 72 hours and (b) no current pneumonia. Upon obtaining informed consent, subjects were randomized into experimental and control groups. Both groups received usual hospital care, that is, daily oral care using cotton swabs. The experimental group additionally received a twice- daily oral care protocol of toothbrushing with purified water, elevating the head of the bed, and before-and-after hypopharyngeal suctioning. The control group also received twice-daily mock oral care (elevating the head of the bed, moisturizing the lips, and before-and-after hypopharyngeal suctioning). VAP was defined by a clinical pulmonary infection score of > 6. Oral hygiene and health was assessed after conclusion of the intervention. Results: Patients (N = 53) were predominantly male (64.2%), mean age was 60.6 years old, and most had received emergency surgery (75.5%). After 7 days of toothbrushing with purified water, cumulative VAP rates were significantly lower in the experimental (17%) than in the control (71%; p <.05) group. The experimental group also had significantly better scores for oral health (p <.05) and plaque index (p <.01). Conclusion/Implication for Practice: Findings suggest that, as an inexpensive alternative to existing protocols, toothbrushing twice daily with purified water reduces VAP and improves oral health and hygiene. [ABSTRACT FROM AUTHOR]

Abstract: The aim of this study was to investigate the prevalence of common geriatric conditions in a tertiary medical center. We conducted a cross-sectional, hospital-wide survey of 455 inpatients, aged 65 and older, from 24 medical and surgical units of a 2200-bed urban academic medical center in Taiwan. Patients were screened in face-to-face interviews for 15 geriatric conditions. The prevalence of geriatric conditions was determined and compared by medical versus surgical services. Our sample of participants had a mean age of 75.3±6.1 years (±S.D.), range=65–92. The prevalence of geriatric conditions ranged from 5% (pressure ulcers) to 57% (polypharmacy; taking>5 prescriptions). The majority was visually impaired (74%) and complained of sleep disturbance during their hospital stay (58%). Prevalence rates of certain geriatric conditions differed significantly between medical and surgical units, suggesting that care should address not only common conditions but also those with higher rates on different units. Furthermore, high rates of geriatric conditions indicate strong needs for care that does not fit into traditional disease models of medicine. Care should be better targeted to address different risks for geriatric conditions of medical versus surgical geriatric inpatients in acute care settings. [Copyright &y& Elsevier]

Background: Identifying shared common risk factors of geriatric syndromes is clinically useful in designing a unified approach to optimizing geriatric care. Objectives: The purpose of this study was to identify older Taiwanese inpatients' common shared risk factors among seven distinct geriatric syndromes: malnutrition, depression, cognitive impairment, functional dependence, incontinence, pressure ulcers, and dehydration. Method: A cross-sectional, hospital-wide survey was conducted to enroll inpatients (N = 455) older than 65 years and admitted to 24 medical and surgical units in a 2,200-bed urban academic medical center in northern Taiwan. Malnutrition was defined as a Mini-Nutritional Assessment score less than 17.5, depression was defined as a Geriatric Depression Scale score more than 10, cognitive impairment was considered a Mini-Mental State Examination score less than 20, and functional dependence was defined as a Barthel Index score less than 50. Incontinence, pressure ulcers, and dehydration were extracted from patients' medical records. Results: Participants had a mean age of 75.3 years (SD = 6.1 years, range = 65-92 years). The prevalence of geriatric syndromes ranged from 5% (pressure ulcers) to 33% (malnutrition). The selected geriatric syndromes were shown through logistic regression analysis to be predicted by female gender (odds ratio [OR] - 1.57-2.75), functional status (OR = 0.94-0.99), cognitive status (OR = 0.82-0.95), nutritional status (OR = 0.74-0.93), and depressive symptoms (OR = 1.07-1.26), supporting the notion of shared risk factors in geriatric syndromes. Conclusions: The findings support the theory that common geriatric syndromes have a shared set of risk factors--female gender, depressive symptoms, and functional, cognitive, and nutritional status. Revising care to target these shared risk factors in preventing common geriatric syndromes is theoretically sound. [ABSTRACT FROM AUTHOR]

Summary: The Drosophila MSL complex associates with active genes specifically on the male X chromosome to acetylate histone H4 at lysine 16 and increase expression approximately 2-fold. To date, no DNA sequence has been discovered to explain the specificity of MSL binding. We hypothesized that sequence-specific targeting occurs at “chromatin entry sites,” but the majority of sites are sequence independent. Here we characterize 150 potential entry sites by ChIP-chip and ChIP-seq and discover a GA-rich MSL recognition element (MRE). The motif is only slightly enriched on the X chromosome (∼2-fold), but this is doubled when considering its preferential location within or 3′ to active genes (>4-fold enrichment). When inserted on an autosome, a newly identified site can direct local MSL spreading to flanking active genes. These results provide strong evidence for both sequence-dependent and -independent steps in MSL targeting of dosage compensation to the male X chromosome. [Copyright &y& Elsevier]

Presents research notes related to heart failure. Use of beta-andrenergic receptor blocker for heart failure; Cause of the enlargement of the heart; Benefits of the drug statins.

The article presents information related to some important researches. After a meal, pancreatic secretion of insulin tells peripheral tissues, mainly skeletal muscle and fat, to take up glucose. In type 2 diabetes, this signal is impaired by "resistance" of peripheral tissues to insulin. In 1995, researchers were quietly introduced to a molecule, now known as adiponectin, that would later emerge as a promising link between obesity and insulin resistance. The way people think about metabolic regulation has been changed, with the discovery of the transcriptional coactivator PGC-1α in 1998.

Presents several research-based news items as of May 05, 2001. Causes of congenital viral diseases; Treatment prescribed for schizophrenia; Development of a novel gene-targeting procedure to create mice that more closely recapitulate the spontaneous oncogene activation observed in human cancers.

Presents news and developments related to medical care as of March 2001. Role of chemokines in the HIV pathogenesis; Significance of leptin in regulating food intake and energy.