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16. Emergence of bla NDM– 1-Carrying Aeromonas caviae K433 Isolated From Patient With Community-Acquired Pneumonia.

Author

Luo, Xinhua, Mu, Kai, Zhao, Yujie, Zhang, Jin, Qu, Ying, Hu, Dakang, Jia, Yifan, Dai, Piaopiao, Weng, Jian, Wang, Dongguo, and Yu, Lianhua

Subjects
MOBILE genetic elements, COMMUNITY-acquired pneumonia, AEROMONAS, HOMOLOGY (Biology), DRUG resistance in bacteria, CARBAPENEMASE
Abstract

To demonstrate the detailed genetic characteristics of a bla NDM–1-carrying multidrug-resistant Aeromonas caviae strain, the complete genome of the A. caviae strain K433 was sequenced by Illumina HiSeq and Oxford nanopore platforms, and mobile genetic elements associated with antibiotic resistance genes were analyzed by a series of bioinformatics methods. A. caviae K433 which was determined to produce class B carbapenemase, was resistant to most antibiotics tested except amikacin. The genome of K433 consisted of a chromosome cK433 (6,482-kb length) and two plasmids: pK433-qnrS (7.212-kb length) and pK433-NDM (200.855-kb length), the last being the first investigated bla NDM-carrying plasmid from Aeromonas spp. By comparison of the backbone and MDR regions from the plasmids studied, they involved a highly homologous sequence structure. This study provides in-depth genetic insights into the plasmids integrated with bla NDM-carrying genetic elements from Aeromonas spp. [ABSTRACT FROM AUTHOR]

Published
2022
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23. Chromosomal Integration of Huge and Complex bla NDM-Carrying Genetic Elements in Enterobacteriaceae.

Author

Luo, Xinhua, Yin, Zhe, Zeng, Lijun, Hu, Lingfei, Jiang, Xiaoyuan, Jing, Ying, Chen, Fangzhou, Wang, Dongguo, Song, Yajun, Yang, Huiying, and Zhou, Dongsheng

Subjects
MOBILE genetic elements, TRANSPOSONS, DNA insertion elements, ENTEROBACTERIACEAE, MULTIDRUG resistance, INTEGRONS, HEAVY metals
Abstract

In this study, a detailed genetic dissection of the huge and complex bla NDM-carrying genetic elements and their related mobile genetic elements was performed in Enterobacteriaceae. An extensive comparison was applied to 12 chromosomal genetic elements, including six sequenced in this study and the other six from GenBank. These 12 genetic elements were divided into five groups: a novel IME Tn 6588 ; two related IMEs Tn 6523 (SGI1) and Tn 6589 ; four related ICEs Tn 6512 (R391), Tn 6575 (ICE Pvu ChnBC22), Tn 6576 , and Tn 6577 ; Tn 7 and its derivatives Tn 6726 and 40.7-kb Tn 7 -related element; and two related IMEs Tn 6591 (GI sul2) and Tn 6590. At least 51 resistance genes, involved in resistance to 18 different categories of antibiotics and heavy metals, were found in these 12 genetic elements. Notably, Tn 6576 carried another ICE Tn 6582. In particular, the six bla NDM-carrying genetic elements Tn 6588 , Tn 6589 , Tn 6575 , Tn 6576 , Tn 6726 , and 40.7-kb Tn 7 -related element contained large accessory multidrug resistance (MDR) regions, each of which had a very complex mosaic structure that comprised intact or residual mobile genetic elements including insertion sequences, unit or composite transposons, integrons, and putative resistance units. Core bla NDM genetic environments manifested as four different Tn 125 derivatives and, notably, two or more copies of relevant Tn 125 derivatives were found in each of Tn 6576 , Tn 6588 , Tn 6589 , and 40.7-kb Tn 7 -related element. The huge and complex bla NDM-carrying genetic elements were assembled from complex transposition and homolog recombination. Firstly identified were eight novel mobile elements, including three ICEs Tn 6576 , Tn 6577 , and Tn 6582 , two IMEs, Tn 6588 and Tn 6589 , two composite transposons Tn 6580a and Tn 6580b , and one integron In1718. [ABSTRACT FROM AUTHOR]

Published
2021
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24. Optimum Application Time and Application Rate of Nitrogen Fertilizer in Brassica napus L

Author

HE, Junping, ZHANG, Shufen, ZHU, Jiacheng, CAO, Jinhua, WEN, Yancheng, CAI, Dongfang, ZHAO, Lei, WANG, Dongguo, and WANG, Jianping

Subjects
Brassica napus L., Nitrogen fertilizer application, Yield, Economic traits, Soil fertility, Agribusiness
Abstract

To find proper nitrogen fertilizer application mode of rapeseed (Brassica napus L.), promote the high and stable yield of rapeseed, field experiment was carried out to study the effect of nitrogen fertilizer application modes on the yield and economic traits of Fengyou 10 rapeseed planted in two different fertility soils, and characteristics and differences of responses of different fertility soils to nitrogen fertilizer application modes were compared and analyzed. The results showed that the yield and related economic traits of rapeseed treated by nitrogen fertilizer were higher than the control group; in the nitrogen fertilizer treatment group, when the nitrogen fertilizer was applied as the base fertilizer and wintering fertilizer at the ratio of 7:3, the rapeseed yield was higher than the control group and reached the highest; when the nitrogen fertilizer was applied as the base fertilizer, wintering fertilizer, and bud fertilizer at the ratio of 5:3:2, the rapeseed yield was significantly higher than the control group but was the lowest. These indicate that sufficient nitrogen supply is the basis for normal growth and development of rape seedlings. When the pure nitrogen fertilizer was at 192 kg/ha and the ratio of base fertilizer and wintering fertilizer was 7:3, it can better coordinate the high yield of rapeseed and overall use of nitrogen fertilizer, and obtain higher rapeseed yield.

Published
2018
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28. CircPUM1 promotes hepatocellular carcinoma progression through the miR‐1208/MAP3K2 axis.

Author

Zhang, Yaqiong, Wang, Dongguo, Zhu, Tao, Yu, Jin, Wu, Xiaoyu, Lin, Weidong, Zhu, Minqi, Dai, Yingjie, and Zhu, Jie

Subjects
HEPATOCELLULAR carcinoma, DIAGNOSIS, PROTEIN expression, ONCOGENES, IN vivo studies
Abstract

Hepatocellular carcinoma (HCC) is a common disease with a significant mortality, and there is no effective treatment for advanced patients. Growing evidence indicates that circRNAs are closely related to HCC progression, may be used as biomarkers and targets for the diagnosis and treatment of HCC. Recent researches have shown that circPUM1 may play an oncogene role in a variety of human cancers, but its role in HCC development has not been reported. Our study found that circPUM1 could promote the proliferation, migration and invasion of HCC cells in vitro. In addition, in vivo studies showed that circPUM1 could increase the development of HCC tumours and regulate the expression of EMT‐related proteins. Furthermore, we demonstrated that circPUM1 could promote the development of HCC by up‐regulating the expression of MAP3K2 via sponging miR‐1208. Our study suggested that circPUM1 may be a potential therapeutic target for HCC. [ABSTRACT FROM AUTHOR]

Published
2021
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29. Physiological and anatomical changes in two rapeseed (Brassica napusL.) genotypes under drought stress conditions

Author

Zhu, Jiacheng, Cai, Dongfang, Wang, Jianping, Cao, Jinhua, Wen, Yancheng, He, Junping, Zhao, Lei, Wang, Dongguo, and Zhang, Shufen

Abstract

Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed (Brassica napusL.) varieties. The present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes, P287 (drought-tolerant) and T88 (drought-sensitive) under three intensities of drought stress. All physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress conditions. At the fourth-leaf stage, the relative water content, chlorophyll content, protein content, malondialdehyde content, and the activities of peroxidase and catalase in P287 were significantly higher than those in T88, particularly under severe drought conditions. After rehydration, all physiological indexes recovered rapidly, especially in P287. In addition, under drought stress, compared with T88, P287 had thicker palisade tissue, thinner spongy tissue, higher ratio of chloroplast length to chloroplast width, higher stomatal density and stomatal closure rate. Overall, the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions.

Published
2021
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46. Fine Mapping of Lobed‐Leaf Genes in Two Brassica napusLines Using SLAF Sequencing

Author

Wen, Yancheng, Zhang, Shufen, Wang, Jianpin, He, Junping, Cai, Dongfang, Zhao, Lei, and Wang, Dongguo

Abstract

Plant ideotypes may be useful for increasing yield in Brassica napusL., and leaves play an important role in the development of ideotypes. Previously, we bred two lobed‐leaf B. napuslines, ll1 and ll2. In the present study, we report the fine mapping of lobed‐leaf genes in ll1and ll2and annotate their putative functions. We conduct specific‐locus amplified fragment sequencing (SLAF‐seq) to determine the candidate intervals. Crosses between two lobed‐leaf lines ll1 and ll2 and their corresponding wild types el1 and el2 were used to generate two sets of F1and F2populations. Genome‐wide comparison of single‐nucleotide polymorphism profiles between lobed‐ and entire‐leaf bulks identified ll1and ll2candidate intervals on ChrA10. Lobed‐leaf gene ll1was located in two regions within genomic positions 16,055,155 to 16,158,378 (100 kb in size) and 16,651,387 to 17,120,610 (470 kb in size), whereas the ll2gene was located within the genomic region of 16,767,957 to 17,024,885 (260 kb in size), which overlapped with the second region of ll1. These results suggest that the lobed‐leaf candidate genes for ll1and ll2are alleles. Basic Local Alignment Search Tool (BLAST) analysis identified 139 genes and 49 genes in the associated regions of ll1and ll2, respectively. Approximately 135 genes in ll1 and 49 genes in ll2 could be functionally annotated, with the majority playing important roles in the regulation of leaf margin and leaf growth. These studies provide useful information for constructing ideotypes and breeding high‐yielding B. napushybrids.

Published
2018
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