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COVID-19 sequelae are long-term symptoms of COVID-19. Cardiovascular disease is not only a risk factor for the occurrence of COVID-19 sequelae but also a potential result directly or indirectly caused by COVID-19 infection.

Infections caused by multi-drug resistant organisms(MDRO)is one of the most common nosocomial infection worldwide. Scientific nursing interventions are effective to reduce the incidence of MDRO infection in patients and improve patient satisfaction during treatment. It is also crucial to improve the awareness and importance of MDRO infection among nursing staff, in order to take targeted nursing measures to reduce the incidence of MDRO infection and improve patient satisfaction. This article reviewed studies on the integrated Traditional Chinese and western medicine nursing in prevention and treatment of MDRO infection, and provided reference for clinical prevention and control of MDRO infection. (多重耐药菌(MDRO)感染是全球最常见的医院感染之一。科学有效的护理干预措施是降低MDRO感染发生率和提高患者满意度的有效手段。同时, 提高护理人员对MDRO感染的认知度和重视度, 采取针对性护理措施, 对降低MDRO感染发生率和提高患者满意度至关重要。本文对中西医结合护理干预在MDRO感染预防和治疗中的应用研究进行综述, 旨在为临床制定医院感染预防措施提供参考。)

The main components of sandalwood heartwood essential oil are terpenoids, approximately 80% of which are α-santalol and β-santalol. In the synthesis of the main secondary metabolites of sandalwood heartwood, the key gene, santalene synthase (SaSSY), can produce α-santalene and β-santalene by catalyzed (E, E)-FPP. Furthermore, santalene is catalyzed by the cytochrome monooxygenase SaCYP736A167 to form sandalwood essential oil, which then produces a fragrance. However, the upstream regulatory mechanism of the key gene santalene synthase remains unclear. In this study, SaSSY (Sal3G10690) promoter transcription factors and SaSSY cis-elements were screened. The results showed that the titer of the sandalwood cDNA library was 1.75 × 107 CFU/mL, 80% of the inserted fragments identified by PCR were over 750 bp in length, and the positivity rate of the library was greater than 90%. The promoter region of the SaSSY gene was shown to have the structural basis for potential regulatory factor binding. After sequencing and bioinformatics analysis, we successfully obtained 51 positive clones and identified four potential SaSSY transcriptional regulators. Sal6G03620 was annotated as the transcription factor MYB36-like, and Sal8G07920 was annotated as the small heat shock protein HSP20 in sandalwood. Sal1G00910 was annotated as a hypothetical protein of sandalwood. Sal4G10880 was annotated as a homeobox-leucine zipper protein (ATHB-15) in sandalwood. In this study, a cDNA library of sandalwood was successfully constructed using a yeast one-hybrid technique, and the transcription factors that might interact with SaSSY gene promoters were screened. This study provides a foundation for exploring the molecular regulatory mechanism involved in the formation of sandalwood heartwood. [ABSTRACT FROM AUTHOR]

The major mechanism of antibody-mediated neutralization of the Middle East respiratory syndrome coronavirus (MERS-CoV) involves competition with the cellular receptor dipeptidyl peptidase 4 (DPP4) for binding to the receptor-binding domain (RBD) of the spike (S) glycoprotein. Here, we report a unique epitope and unusual neutralizing mechanism of the isolated human antibody MERS-4. Structurally, MERS-4 approached the RBD from the outside of the RBD-DPP4 binding interface. Such binding resulted in the folding of the β5-β6 loop toward a shallow groove on the RBD interface critical for accommodating DPP4. The key residues for binding are identified through site-directed mutagenesis. Structural modeling revealed that MERS-4 binds to RBD only in the "up" position in the S trimer. Furthermore, MERS-4 demonstrated synergy with several reported antibodies. These results indicate that MERS-4 neutralizes MERS-CoV by indirect rather than direct competition with DPP4. This mechanism provides a valuable addition for the combined use of antibodies against MERS-CoV infection.

The aim of this study is to improve the compressive strength of oil absorbent concrete (OAC) and to encourage its use in slope protection projects. This study used fly ash and slag produced in thermal power plants to substitute cement in significant amounts to prepare oil absorbent concrete (OAC). The water–cement ratios were set at 0.4, 0.5, and 0.6 and the sand rates were set at 30%, 35%, and 40% to investigate the effects of these factors on the oil absorption properties of the concrete, the variation of the oil absorption rate over time, and the compressive strengths at 28 days, 60 days, and 90 days. The compressive strength of oil absorbent concrete was improved by incorporating seashell powder (SC), alkali-modified seashell powder (SSC), and acid–base-modified seashell powder (CSC). The results showed that the optimal water–cement ratio for comprehensive oil absorption performance and compressive strength was 0.5, while the optimal sand ratio was 0.35. Compared with ordinary concrete, the oil absorption performance improved by 58.69%. The oil absorption rate decreased gradually over time. However, the oil absorption time could be effectively extended and the oil absorption performance could be improved by the addition of a silane modifier. The best method for seashell modification was acid–base modification. The compressive strength reached 14.32 Mpa at 28 days and 17.45 Mpa at 90 days, which was 19.62% higher than that of OAC. Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), and X-ray diffraction (XRD) were used to analyze the microstructure of OAC. It was discovered that the inclusion of CSC caused a reaction with hydrocalumite in the concrete, resulting in the formation of alumohydrocalcite. Additionally, Ca(OH)2 in CSC facilitated the hydration reaction of mineral admixtures like fly ash and slag. At 28 days, more amorphous gels (C-S-H, C-(A)-S-H) and Aft were produced. The three components were combined to enhance the bonding between the cementitious materials and the aggregates, resulting in a denser internal structure of the OAC and improving its strength. This study promotes the use of OAC in slope protection projects. [ABSTRACT FROM AUTHOR]