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Background: Docosahexaenoic Acid (DHA) is an extensively used nutrition supplement in dairy food because of its beneficial effects on cognition. To find an effective DHA intervention for the synapses in the cortex during this period, this study aimed to use targeted lipidomics to evaluate the lipid composition of prefrontal-cortex (PFC) tissue in different DHA interference methods. Methods: Analyzed samples were taken from interfering feeding Bama pigs (BPs) (3 months) fed with soybean oil (Group B), blended oil (Group M), naturally DHA-supplemented milk with blended oil (Group OM), and DHA from fish oil (FO) with blended oil (Group Y). We also examined the protein expression levels of BDNF, GAP43, and MBP. Results: The lipidomics analysis identified 80 different related negative-ion lipid content and filtered the biomarker lipids in PFC tissue. We observed significant lipid composition changes between group Y and other groups, especially for content levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), and sphingomyelin (SM). The same observations were made from mRNA and protein expressions related to lipid transportation, phosphatidylserine (PS) synthetase, and synaptic plasticity in PFC tissues between group Y and other groups, including the mRNA expression levels of CD36, BDNF, and PTDSS1. The analysis of protein expression levels showed that the metabolism mode of DHA intervention from FO benefited the PFC, PS metabolism, and PFC synaptic plasticity of infants. Conclusions: The results highlight further prospects for the DHA intervention mode, which provides new routes for other studies on polyunsaturated-fatty-acid (PUFA) interference for infants. [ABSTRACT FROM AUTHOR]

Introduction: Synbiotics have revealed the possibility of improving constipation through gut microbiota. The synergistic efficacy of Bifidobacterium animalis subsp. lactis BL-99 (BL-99) and fructooligosaccharide (FOS) on constipation have not been investigated. Methods: Loperamide-induced constipated mice model was established to explore the effect of BL-99, FOS, and BL-99+FOS on changes of defecation-related parameters, gut microbiota and metabolites. Results and discussion: The results showed that BL-99, FOS, and BL-99+FOS each alleviated constipation, with the synbiotic showing significant efficacy in the first black stool defecation time, fecal number, fecal weight, and the gastrointestinal transit rate (P < 0.05). Additionally, significant increased in serum 5-HT and IL-10 were observed in the BL-99+FOS group, alongside an increased relative abundance of Lachnospiraceae_NK4A136_group, Blautia , and Clostridium sensu stricto 1 , while significantly reducing the relative abundance of Alistipes and Bacteroides. These changes facilitated alterations in short-chain fatty acids (SCFAs) metabolism, and were closely associated with the expression of genes related to the 5-HT pathway and the modulation of serum inflammatory factors. This study provides a theoretical basis for BL-99 and FOS synbiotics to improve constipation by regulating the gut microbiota and metabolites. [ABSTRACT FROM AUTHOR]

Chicken yolk immunoglobulin (IgY) is a natural immunologically active antibody extracted from egg yolk and can be used as a natural dietary supplement for the treatment of inflammation and damage to the intestines. In our study, IgY was embedded in a double emulsion (W/O/W; DE) to explore the therapeutic effect of the embedded IgY on Lipopolysaccharide (LPS)-induced jejunal injury in mice. The results showed that W/O/W-embedded IgY as a dietary supplement (IgY + DE) attenuated LPS-induced damage to mouse small intestinal structures and protected the integrity of the jejunal mucosal barrier. IgY + DE increased the amount of related transcription factors (Math1, Spdef, Elf3, and Klf4) and promoted thrush cell differentiation. IgY + DE ameliorated LPS-induced reduction in mucin quantity and markers. It promoted the expression of Muc1 and Muc2 and increased the mRNA expression levels of Muc1, Muc2, Muc3, Muc4, Muc13, and Agr2 (p < 0.05). IgY + DE increased the expression of several glycosyltransferases involved in mucin glycosylation. IgY + DE also neutralized the LPS attack on the expression of jejunal inflammatory factors IL-1β, IL-6, IL-4, and TNF-α. In conclusion, the IgY-embedded double emulsion can be used as a dietary supplement for immunotherapy to prevent LPS-induced jejunal injury in mice. [ABSTRACT FROM AUTHOR]

Oxidative stress is closely linked to aging. Probiotics, whether viable or heat-inactivated, have shown antioxidant properties; however, their effect and mechanism of action in reducing oxidative stress during aging remains underexplored. This study examined the effects of viable and heat-inactivated Lactiplantibacillus plantarum TY-Y10 (L. plantarum TY-Y10) on D-galactose (D-gal)-induced aging in mice, aiming to uncover potential anti-aging mechanisms. Mice were induced to age with D-gal injections, then treated with sodium ascorbate (positive control) or varying doses of L. plantarum TY-Y10 for eight weeks. After treatment, oxidative stress markers, gut microbiota, and liver health were analyzed. Results showed that L. plantarum TY-Y10 decreased malondialdehyde (MDA) and inflammatory markers while increasing antioxidant levels (glutathione, superoxide dismutase, catalase and glutathione peroxidase). Liver damage was reduced, and expression of Nrf2 and related antioxidant enzymes improved. Additionally, L. plantarum TY-Y10 enhanced the abundance of short-chain fatty acid-producing bacteria, boosting fecal short-chain fatty acid levels. In short, both viable and heat-inactivated L. plantarum TY-Y10 mitigated oxidative stress in aging mice by modulating gut microbiota and activating liver antioxidant pathways through the gut-liver axis. [ABSTRACT FROM AUTHOR]

As an important part of public buildings, the school’s energy consumption and carbon emissions have received widespread attention. Relying on the existing building energy conservation situation and the energy consumption of Tianjin Yaohua High school, this paper puts forward the existing problems and energy saving space of high school. Based on the goal of low-carbon transformation, the roof and other utilizable conditions were analyzed, the transformation scheme of low-carbon energy system was brought forward, which realizes clean heating, electric energy replacement and domestic hot water supply with PVT coupling with heat pump. According to calculation, it can realize 70% replacement of school electricity, 100% clean heating and 100% consumption of renewable energy. Based on economic analysis, it has strong feasibility, which provides a reference for the subsequent low-carbon transformation of existing high school.

The existing campus has a large building area, a low level of energy saving, less renewable energy utilization, higher energy consumption, and obvious energy-saving space. Based on the energy audit of Nankai University, this paper proposes a low-carbon campus transformation technology system combining building energy saving, clean heat supply and distributed photovoltaic in view of its problems such as high municipal heating energy consumption, low comfort, high electricity bill, and tight electricity consumption. Because the campus has both winter holidays and there is tidal phenomenon in teaching buildings and dormitory buildings, etc., the heating demand is reduced, so its heating and energy-saving space is large. At the same time, the number of campus building floors is low, the roof is conducive to the installation of photovoltaic, it is estimated that the annual power generation of rooftop photovoltaic installation can reach more than 70% of the campus electricity consumption, combined with energy-saving transformation and smart energy management, it is easy to achieve its low-carbon goals.

Non-alcoholic fatty liver disease (NAFLD) is now recognized as the most prevalent liver disease globally. Pea albumin (PA) has demonstrated positive impacts on reducing obesity and improving glucose metabolism. In this research, a mouse model of NAFLD induced by a high-fat diet (HFD) was employed to examine the impact of PA on NAFLD and explore its potential mechanisms. The findings revealed that mice subjected to a HFD developed pronounced fatty liver alterations. The intervention with PA significantly lowered serum TC by 26.81%, TG by 43.55%, and LDL-C by 57.79%. It also elevated HDL-C levels by 1.2 fold and reduced serum ALT by 37.94% and AST by 31.21% in mice fed a HFD. These changes contributed to the reduction in hepatic steatosis and lipid accumulation. Additionally, PA improved insulin resistance and inhibited hepatic oxidative stress and inflammatory responses. Mechanistic studies revealed that PA alleviated lipid accumulation in HFD-induced NAFLD by activating the phosphorylation of AMPKα and ACC, inhibiting the expression of SREBF1 and FASN to reduce hepatic lipogenesis, and increasing the expression of ATGL, PPARα, and PPARγ to promote lipolysis and fatty acid oxidation. These results indicate that PA could serve as a dietary supplement for alleviating NAFLD, offering a theoretical foundation for the rational intake of PA in NAFLD intervention. [ABSTRACT FROM AUTHOR]

With the implementation of China’s two-carbon strategy, how to achieve clean heating, especially the construction of a heating system with electricity as the core, has become a research hotpot in the industry. Among them, air source heat pumps, ground source heat pumps and energy storage have become important technical measures. In view of the capacity increase of urban heat exchange stations, combined with regenerative electric boilers and electricity price reform, this paper uses valley electricity for heat storage, and based on economic analysis, the municipal heat network is increased in flexibility and cleanliness, air energy, coupled energy storage clean and low-carbon heating transformation strategy.

Background A decade ago, we proposed an index of physiological dysregulation based on Mahalanobis distance (DM) that measures how far from the norm an individual biomarker profile is. While extensive validation has been performed, focus was mostly on Western populations with little comparison to developing countries, particularly at a physiological system level. The degree to which the approach would work in other sociocultural contexts and the similarity of dysregulation signatures across diverse populations are still open questions. Methods Using 2 data sets from China and 3 from Western countries (United States, United Kingdom, and Italy), we calculated DM globally and per physiological system. We assessed pairwise correlations among systems, difference with age, prediction of mortality and age-related diseases, and sensitivity to interchanging data sets with one another as the reference in DM calculation. Results Overall, results were comparable across all data sets. Different physiological systems showed distinct dysregulation processes. Association with age was moderate and often nonlinear, similarly for all populations. Mahalanobis distance predicted most health outcomes, although differently by physiological system. Using a Chinese population as the reference when calculating DM for Western populations, or vice versa, led to similar associations with health outcomes, with a few exceptions. Conclusions While small differences were noticeable, they did not systematically emerge between Chinese and Western populations, but rather diffusively across all data sets. These findings suggest that DM presents similar properties, notwithstanding sociocultural backgrounds, and that it is equally effective in capturing the loss of homeostasis that occurs during aging in diverse industrial human populations. [ABSTRACT FROM AUTHOR]

The energy use behavior analysis method can dig out the user’s energy use behavior rules from the energy use big data, thereby improving the quality of the grid-side management service in the integrated energy system. Firstly, it summarizes the characteristics of the integrated energy system and constructs the integrated energy system service system; secondly, it summarizes the data-driven electricity consumption behavior analysis research model. Then, it elaborates on the collection and aggregation of electricity consumption information, and refined user classification. Next, the comprehensive application of energy consumption behavior analysis in load forecasting, demand response modeling and other typical scenarios is deeply analyzed. Finally, the challenges that may be encountered in further research are clarified and the follow-up work is prospected.

Sulfamethoxazole (SMX) is a commonly used antibiotic for both human and animals. The frequent detection of SMX in natural water bodies and sediment has become an issue of great environmental concern due to its potential risk to induce antibiotic resistance in pathogenic bacteria. In the present work, the catalytic wet peroxide oxidation (CWPO) was investigated to remove SMX with sludge-derived carbon (SC) as a cheap alternative catalyst. Different acids were used to modify SC. It was found that SC modified with sulphuric acid (SC-H2SO4) demonstrated the best catalytic activity. The removal efficiency of SMX and TOC was 97.7% and 65.7%, respectively, after 260 min, at pH 5 with a dosage of 220 mg/L H2O2. The effects of temperature, initial pH and H2O2 dosage were also investigated. The study demonstrated that the increase of temperature could significantly improve the degradation of SMX from 10.0% at 20°C to 94.7% at 60°C. [ABSTRACT FROM AUTHOR]