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4 results on '"Wang, Hekong"'

3. ROS-induced ZNF580 expression: a key role for H2O2/NF-κB signaling pathway in vascular endothelial inflammation

Author

Wang HeKong, Ren Dang-li, Zhang MingHua, Zhang Wen-cheng, and Liu JiQin

Subjects
Endothelium, medicine.medical_treatment, Clinical Biochemistry, Biology, chemistry.chemical_compound, Pyrrolidine dithiocarbamate, Cell Line, Tumor, medicine, Humans, Molecular Biology, Transcription factor, chemistry.chemical_classification, Inflammation, Reactive oxygen species, Interleukin-8, NF-kappa B, NF-κB, Cell Biology, General Medicine, Hydrogen Peroxide, Molecular biology, Cell biology, Up-Regulation, Endothelial stem cell, medicine.anatomical_structure, Cytokine, chemistry, Endothelium, Vascular, Signal transduction, Reactive Oxygen Species, Signal Transduction, Transcription Factors
Abstract

ZNF580, a newly found C2H2 zinc finger transcription factor, was first described by Zhang (GenBank ID: AF184939). Emerging evidence has suggested that reactive oxygen species (ROS) play an important role in redox-sensitive signal transduction, and the vascular endothelium plays a critical role in the vascular inflammatory response. In this communication, we present evidence for the potential role of ZNF580 in hydrogen peroxide (H2O2)-regulated inflammation-related signaling pathways. In a human endothelial cell hybridoma line (EA.hy926), ZNF580 levels were markedly upregulated with H2O2 stimulation in different concentrations (0–400 μM) and at different time-points (0–6 h). H2O2 promoted the rapid translocation of p65 from the cytoplasm into the nucleus according to immunocytochemistry staining. In subsequent research, inhibition of NF-κB by pyrrolidine dithiocarbamate (PDTC, a selective chemical inhibitor of NF-κB) was shown to block the upregulated expression of ZNF580 that was induced by H2O2. Furthermore, transient transfection of ZNF580 resulted in an increase of the pro-inflammatory cytokine interleukin-8 (IL-8) 3.01 ± 0.05 folds according to real-time RT-PCR and ELISA assays, which also showed significantly enhanced motility of human acute monocytic leukemia cells (THP-1). These results suggest that H2O2 upregulates the expression of ZNF580 via the NF-κB signaling pathway, and overexpression of ZNF580 plays a critical role in augmenting the release of pro-inflammatory cytokine IL-8.

Published
2011

4. m6A demethylase FTO facilitates tumor progression in lung squamous cell carcinoma by regulating MZF1 expression.

Author

Liu, Jiqin, Ren, Dangli, Du, Zhenhua, Wang, Hekong, Zhang, Hua, and Jin, Ying

Subjects
*LUNG cancer, *SQUAMOUS cell carcinoma, *DEMETHYLASE, *CANCER invasiveness, *CANCER cell proliferation
Abstract

N 6 -Methyladenosine (m 6 A) represents the most prevalent internal modification in mammalian mRNAs. Emerging evidences suggest that m 6 A modification is profoundly implicated in many biological processes, including cancer development. However, limited knowledge is available about the functional importance of m 6 A in lung cancer. In this study, by data mining The Cancer Genome Atlas (TCGA) database, we first identified fat mass- and obesity-associated protein (FTO) as a prognostic factor for lung squamous cell carcinoma (LUSC). Then we showed that FTO, but not other m 6 A modification genes including METTL3, METTL14 and ALKBH5, was the major dysregulated factor responsible for aberrant m 6 A modification in LUSC. Loss-of-function studies suggested that FTO knockdown effectively inhibited cell proliferation and invasion, while promoted cell apoptosis of L78 and NCI-H520 cells. Furthermore, overexpression of FTO, but not its mutant form, facilitated the malignant phenotypes of CHLH-1 cells. Mechanistically, FTO enhanced MZF1 expression by reducing m 6 A levels and mRNA stability in MZF1 mRNA transcript, leading to oncogenic functions. Taken together, our study demonstrates the functional importance of FTO in the tumor progression of LUSC and provides a potential therapeutic target for LUSC treatment. [ABSTRACT FROM AUTHOR]

Published
2018
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