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In order to solve the problems of existing spiral fertilizer apparatuses, such as the variation in cavity filling rate with rotational speed, fluctuation of fertilizer discharge flow, and inability to discharge fertilizer precisely, a triple-head gradually reducing spiral fertilizer apparatus is designed, which gradually compresses fertilizer particles through the triple-head reducing fertilizer spiral structure to achieve complete cavity filling and uniform fertilizer discharge. The main factors that affect the particle motion state and the structural size of the spiral fertilizer through theoretical analysis are determined, and its theoretical fertilizer discharge amount and rotational speed are calculated. Using EDEM (Discrete Element Method Software 2022) to establish a simulation model of a single-head gradually reducing fertilizer apparatus, the spiral lead reduction percentage x1, spiral diameter reduction percentage x2, and rotational speed x3 are determined as experimental factors, and the filling rate μ and spiral torque Yaverage are used as experimental indicators to conduct a simulation study on the secondary universal rotation combination design experiment. The results show that when the rotational speed is 95 r/min, the spiral lead reduction percentage is 60.00~73.21%, the spiral diameter reduction percentage is 86.55~97.05%, the filling rate μ is greater than 95%, and the spiral torque Yaverage is less than 16 N·m. In order to further improve the uniformity of fertilizer discharge and ensure the controllable accuracy of fertilizer discharge, comparative verification experiments are conducted on single-, double-, and triple-head gradually reducing spiral fertilizer discharge devices and ordinary spiral fertilizer discharge devices. The results show that the precision of the gradually reducing spiral fertilizer apparatus is better than that of the ordinary spiral fertilizer apparatus. Moreover, it is determined that the three-head style performed best. The triple-head gradually reducing spiral fertilizer apparatus is also validated by randomly adjusting six rotational speeds. The experiment results show that the average deviation of the fertilizer discharge flow rate of the fertilizer apparatus from the preset value is 3.16%. The two have a minor deviation, and the fertilizer precision is high. Precise control of the amount of fertilizer discharged can be achieved by adjusting the rotational speed, and the research can provide a specific reference for the improved design and precise control of the spiral fertilizer apparatus. [ABSTRACT FROM AUTHOR]

Aims: To measure the therapeutic effect of an anti‐oxidant, edaravone (EDV), or neurotrophic treatment with nerve growth factor (NGF) as an add‐on treatment for alcohol‐related brain damage (ARBD). Design: Multi‐centre, randomised, single‐blinded, comparative clinical trial. Setting and participants: One hundred and twenty‐two inpatients recruited from seven hospitals in different regions of China, all diagnosed with ARBD and aged 18 to 65 years old; among them, only two were female. Intervention and comparator: Patients were randomly assigned to receive one of three treatments for 2 weeks: 40 patients, treatment as usual (TAU: a combination of intramuscular injections of thiamine, intravenous infusions of other B vitamins with vitamin C and oral medication with vitamin E per day); 40, EDV add‐on treatment to TAU (intravenous infusion with 30 mg of EDV twice per day); and 42, NGF add‐on treatment to TAU (intramuscular injection of 20 μg of NGF per day). The patients underwent follow‐up for 24 weeks. Measurements: The primary outcome was the composite score of executive cognitive function in the 2nd week after treatment, which was measured as the mean of the Z scores of the assessments, including the digit symbol substitute test (DSST), digit span memory test‐forward (DST‐F), digit span memory test‐reverse (DST‐R) and space span memory test (SSMT). The secondary outcomes were the composite scores at later follow‐ups, the score for each component of cognitive function, global cognitive function measured by the Montreal Cognitive Assessment (MoCA), craving for alcohol and the safety of the therapies. Findings EDV add‐on treatment improved the composite score of executive cognitive function better than TAU in the 2nd week (adjusted mean difference: 0.24, 95% confidence interval 0.06 to 0.41; P = 0.008), but NGF add‐on treatment did not (adjusted mean difference: 0.07, 95% confidence interval −0.09 to 0.24; P = 0.502). During the follow‐up to 24 weeks, EDV add‐on treatment improved the composite score of executive cognitive function and DST‐R score better than TAU (both P < 0.01). Craving for alcohol was relieved in all three groups. No severe adverse events were observed. Conclusion: The short‐term addition of edaravone to supplementary therapy treatment for alcohol‐related brain damage (ARBD) improved executive cognitive function in patients with ARBD. [ABSTRACT FROM AUTHOR]

Aims: Chronic alcohol exposure leads to persistent neurological disorders, which are mainly attributed to neuroinflammation and apoptosis. Stimulator of IFN genes (STING) is essential in the cytosolic DNA sensing pathway and is involved in inflammation and cellular death processes. This study was to examine the expression pattern and biological functions of STING signaling in alcohol use disorder (AUD). Methods: Cell‐free DNA was extracted from human and mouse plasma. C57BL/6J mice were given alcohol by gavage for 28 days, and behavior tests were used to determine their mood and cognition. Cultured cells were treated with ethanol for 24 hours. The STING agonist DMXAA, STING inhibitor C‐176, and STING‐siRNA were used to intervene the STING. qPCR, western blot, and immunofluorescence staining were used to assess STING signaling, inflammation, and apoptosis. Results: Circulating cell‐free mitochondrial DNA (mtDNA) was increased in individuals with AUD and mice chronically exposed to alcohol. Upregulation of STING signaling under alcohol exposure led to inflammatory responses in BV2 cells and mitochondrial apoptosis in PC12 cells. DMXAA exacerbated alcohol‐induced cognitive impairment and increased the activation of microglia, neuroinflammation, and apoptosis in the medial prefrontal cortex (mPFC), while C‐176 exerted neuroprotection. Conclusion: Activation of STING signaling played an essential role in alcohol‐induced inflammation and mitochondrial apoptosis in the mPFC. This study identifies STING as a promising therapeutic target for AUD. [ABSTRACT FROM AUTHOR]

Nursery trays are essential agricultural tools in rice production. Plastic nursery trays pose problems such as resource waste and environmental pollution. Biomass seedling trays are an effective way to achieve sustainable agricultural development. Previous research has been conducted on biomass seedling tray molding equipment and molding process, but the impact of raw material ratio on seedling tray molding quality and seedling growth is still unclear, and the ratio combination still needs further optimization. In this study, we used slurry concentration, pulp content, adhesive content, and the ratio of straw to cow manure as variables. We selected the bowl hole molding rate and the strong seedling index as evaluation indicators, and carried out biomass seedling tray forming experiments and seedling cultivation experiments. The response surface analysis method was used to optimize the raw material ratio of biomass seedling trays from the perspectives of forming effect and seedling quality. The results show that when the slurry concentration is 30%, the pulp content is 20%, the adhesive content is 530 g, and the mass ratio of straw to cow manure is 2:1; the bowl hole molding rate is 91.03%, and the strong seedling index is 0.22, indicating good molding effect and seedling growth. The verification test results indicate that the theoretical analysis results are accurate, and the model fitting is good. These research results provide a theoretical basis for the preparation of biomass seedling trays and technical support for achieving green development in agriculture. [ABSTRACT FROM AUTHOR]

Background and purpose: Glioma is a frequent primary brain tumor. MicroRNAs (miRNA) have been shown to potentially play a crucial part in tumor development. Based on miRNAs and clinical factors, a model was constructed to predict the glioma prognosis. Methods: The miRNA expression profiles of glioma come from The Cancer Genome Atlas (TCGA, training group) and Chinese Glioma Genome Atlas (CGGA, validation group). Regression analyses of Cox and Lasso were applied to identity miRNAs associated with glioma prognosis in the TCGA database. The miRNAs were combined with clinical factors to construct individualized prognostic prediction models, whose performance was validated in the CGGA database. The role of miRNA in glioma development was investigated by in vitro experiments. Results: We identified five key miRNAs associated with glioma prognosis and constructed a prediction model. The area under ROC curve for predicting 3-year survival of glioma patients in the TCGA and CGGA groups was 0.844 and 0.770, respectively. The nomogram constructed using the miRNA risk scores and clinical factors showed high accuracy of prediction in the TCGA group (C-index of 0.820) and the CGGA group (C-index of 0.722). The miR-196b-5p altered the migration, proliferation, invasion, and apoptosis of glioma cells by regulating target genes, according to in vitro experiments. Conclusions: A miRNA-based individualized prognostic prediction model was constructed for glioma and miR-196b-5p was identified as a potential biomarker of glioma development. [ABSTRACT FROM AUTHOR]