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In this study, free radical oxidation was chosen to induce covalent reaction of gliadin (GL) with epigallocatechin gallate. The effects of different ultrasound (US) powers on the enhancement of the covalent reaction were compared with the aim of improving the bioavailability of polyphenols in protein modification. Covalent modification of polyphenols was verified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), high-performance liquid chromatography (HPLC) and the Folin-Ciocalteu assay. The structural changes of the covalent complexes were analyzed by ultraviolet (UV) spectroscopy and circular dichroism (CD) spectroscopy. The results showed that US disrupted weak intra-protein forces, which caused molecular depolymerization. The hydrophilic/hydrophobic amino acid residues of the protein were shifted and the tertiary structure tended to be looser, and this process altered the distribution of groups on the molecule surface and increased the water solubility of the protein. Furthermore, the shift in the spatial structure of GL resulted in the exposure of more enzyme cleavage sites, making its digestion easier. The synergistic effect of US and free radical oxidation increased the loading of antioxidant molecules onto the covalent complexes, resulting in stronger free radical scavenging activity.

Exploring the role of novel cancer gene BZW1 in lung adenocarcinoma (LUAD) and unveiling associated signalling pathways. Firstly, we conducted a pan‐cancer analysis of BZW1 using multiple databases. Subsequently, leveraging single‐cell data from LUAD, we successfully uncovered potential oncological processes associated with BZW1 and further validated them through experimentation. Simultaneously, we continued to investigate the potential molecular mechanisms underlying the oncological processes mediated by BZW1. Additionally, we employed various machine learning algorithms to construct prognostic models concerning BZW1 and the epithelial‐mesenchymal transition (EMT) process. Our research firstly demonstrated the elevated expression of BZW1 in various cancer cells. Leveraging single‐cell data from LUAD, we identified that BZW1 regulates the occurrence of EMT in LUAD, a phenomenon validated across multiple LUAD cell lines. Moreover, we further discovered that BZW1 regulates LUAD's EMT process through the Wnt/β‐catenin signalling pathway. Lastly, we successfully constructed prognostic models using BZW1‐related genes and EMT genes. [ABSTRACT FROM AUTHOR]

The intratumor microbiota results in the immunosuppressive microenvironment and facilitates tumor growth and metastasis. However, developing a synergistic therapy with antitumor, antibacterial, and antimetastatic effects faces enormous challenges. Here, we propose an innovative metal chelation therapy to effectively eliminate tumor and intratumor bacteria and suppress tumor metastasis. Different from traditional chelation therapy that only consumes metal elements, this therapy not only eliminates the crucial metal elements for tumor metabolism but also releases new metal ions with antitumor and antibacterial properties. Based on the high demand for copper in breast cancer, we prepare a fibrous therapeutic nanoagent (Zn‐PEN) by chelating the copper chelator D‐Penicillamine (D‐PEN) with Zn2+. Firstly, Zn‐PEN achieves dual inhibition of oxidative phosphorylation (OXPHOS) and glycolysis metabolism in breast cancer through copper depletion and Zn2+ activated cGAS‐STING pathway, thus inducing tumor cell death. Secondly, Zn‐PEN has the capability to eradicate Fusobacterium nucleatum (F. nucleatum) in breast cancer, thereby mitigating its immunosuppressive impact on the tumor microenvironment. Finally, Zn‐PEN effectively inhibits tumor metastasis through multiple routes, including the inhibition of epithelial‐mesenchymal transition (EMT) process, activation of cGAS‐STING pathway, and elimination with F. nucleatum. Therefore, we verify the feasibility of Zn‐PEN mediated metal chelation therapy in a 4T1 model infected with F. nucleatum, providing a new therapeutic strategy for inhibiting tumor metastasis. [ABSTRACT FROM AUTHOR]

In this work, four kinds of silica with similar textural properties but different particle size (PS) from 6 to 60 μm were selected as support to prepare silyl‐chromate/VOx/silica bimetallic catalysts for ethylene polymerization, thus making it possible to conduct convincing investigation on the effects of catalyst PS over catalytic behaviors and polymer properties. It is found, owing to mass‐ and heat‐transfer limitations, the catalysts with smaller PS showed higher initial activity, while the deactivation in the later stage was more remarkable. Meanwhile, catalysts with smaller PS synthesized bimodal ultrahigh molecular weight polyethylene/high‐density polyethylene in‐reactor blends (UHMWPE/HDPE IRBs) with higher molecular weight (MW) and narrower molecular weight distribution (MWD). Four PE sample couples with MW increasing from 5 × 105 to 10 × 105 g mol−1 and PS from 40 to 460 μm were selected for tensile and rheological tests. For each couple with similar MW and MWD, the sample with smaller PS always had larger tensile strength and breaking elongation. The cryogenically fractured surface of the samples made by smaller PE particles showed fewer delamination defects and unfused small particles. The rheological results indicate that higher homogeneity was achieved for the sample with smaller PS, which was reflected by the unexpected higher viscosity and storage modulus. [ABSTRACT FROM AUTHOR]

Autophagy, a lysosome‐involved degradation pathway, as a self‐protective cellular process, always weakens the efficiency of tumor therapies. Herein, for the first time, the biodegradable copper (Cu) ions doped layered double hydroxide (Cu‐LDH) nanoparticles are reported for cancer immunotherapy via lysosomal rupture‐mediated "Broken Window Effect". Only injection of Cu‐LDH single therapeutic agent achieves various organelles destruction after lysosomal rupture, as well as the abnormal aggregation of Cu ions in tumor cells for cuproptosis and pyroptosis. More importantly, autophagy inhibition caused by lysosomal rupture improves Cu ions overload‐mediated cuproptosis and pyroptosis by blocking the lysosome‐mediated bulk degradation pathway, leading to good anti‐tumor immune responses and ultimately high‐efficiency tumor growth inhibition. This lysosomal rupture‐mediated "Broken Window Effect" provides a new paradigm for the autophagy enhanced tumor therapy. [ABSTRACT FROM AUTHOR]

In the framework of the Polyakov quark-meson model with two flavors, the bubble dynamics of a first-order phase transition in the region of high density and low temperature are investigated by using the homogeneous thermal nucleation theory. In mean-field approximation, after obtaining the effective potential with the inclusion of the fermionic vacuum term, we build a geometric method to search two existing minima, which can be actually connected by a bounce interpolated between a local minimum to an adjacent global one. For both weak and strong first-order hadron quark phase transitions, as fixing the chemical potentials at  μ = 306 MeV  and  μ = 310 MeV , the bubble profiles, the surface tension, the typical radius of the bounce, and the saddle-point action as a function of temperature are numerically calculated in the presence of a nucleation bubble. It is found that the surface tension remains at a very small value even when the density is high. It is also noticed that the deconfinement phase transition does not change the chiral phase transition dramatically for light quarks and phase boundaries for hadron and quark matter should be resized properly according to the saddle-point action evaluated on the bounce solution. [ABSTRACT FROM AUTHOR]

Floating structures are subject to complex marine conditions. To ensure their safety, reliability analysis needs to be conducted during the design phase. However, because of the complexity of traditional full long-term analysis, the environmental contour method (ECM) based on the inverse reliability method, which can combine accuracy and efficiency, is extensively used. Due to the unique environment in the South China Sea, the probabilistic characteristics of three-dimensional (3D) environmental parameters of wind, wave and current are investigated. The ECs of the target sea are established via the ECM based on both the inverse first-order reliability method (IFORM) and inverse second-order reliability method (ISORM). It is found that the sea state forecasted by ISORM is more extreme and may lead to a more conservative design than IFORM. Furthermore, the wind–wave–current combination coefficient matrixes developed using the 3D ECs are proposed for the design of FOWTs in the South China Sea. The validity and practicality of the contours and matrixes are tested by using a floating offshore wind turbine (FOWT) as a numerical example. Then, the short-term response of the structure under the combined wind, wave and current conditions is calculated, providing a theoretical reference for the design of FOWTs. [ABSTRACT FROM AUTHOR]