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14. Classification of PRSS1 variants responsible for chronic pancreatitis: An expert perspective from the Franco-Chinese GREPAN Study Group

Author

Abrantes, Amandine, Aguilera Munoz, Lina, Albouys, Jérémie, Alric, Laurent, Amiot, Xavier, Archambeaud, Isabelle, Audiau, Solène, Bastide, Laetitia, Baudon, Julien, Bellaiche, Guy, Bellon, Serge, Bertrand, Valérie, Bideau, Karine, Billiemaz, Kareen, Billioud, Claire, Bonnefoy, Sabine, Borderon, Corinne, Bournet, Barbara, Breton, Estelle, Brugel, Mathias, Buscail, Louis, Cadiot, Guillaume, Camus, Marine, Causse, Xavier, Chamouard, Patrick, Chaput, Ulriikka, Cholet, Franck, Ciocan, Dragos Marius, Clavel, Christine, Coffin, Benoit, Coimet-Berger, Laura, Creveaux, Isabelle, Culetto, Adrian, Daboussi, Oussama, De Mestier, Louis, Degand, Thibault, D'Engremont, Christelle, Denis, Bernard, Dermine, Solène, Desgrippes, Romain, Drouet D'Aubigny, Augustin, Enaud, Raphaël, Fabre, Alexandre, Gargot, Dany, Gelsi, Eve, Gentilcore, Elena, Gincul, Rodica, Ginglinger-Favre, Emmanuelle, Giovannini, Marc, Gomercic, Cécile, Gondran, Hannah, Grainville, Thomas, Grandval, Philippe, Grasset, Denis, Grimaldi, Stéphane, Grimbert, Sylvie, Hagege, Hervé, Heissat, Sophie, Hentic, Olivia, Herber-Mayne, Anne, Hervouet, Marc, Hoibian, Solene, Jacques, Jérémie, Jais, Bénédicte, Kaassis, Mehdi, Koch, Stéphane, Lacaze, Elodie, Lacroute, Joël, Lamireau, Thierry, Laurent, Lucie, Le Guillou, Xavier, Le Rhun, Marc, Leblanc, Sarah, Levy, Philippe, Lievre, Astrid, Lorenzo, Diane, Maire, Frédérique, Marcel, Kévin, Matias, Clément, Mauillon, Jacques, Morgant, Stéphanie, Moussata, Driffa, Muller, Nelly, Nambot, Sophie, Napoleon, Bertrand, Olivier, Anne, Pagenault, Maël, Pelletier, Anne-laure, Pennec, Olivier, Pinard, Fabien, Pioche, Mathieu, Prost, Bénédicte, Queneherve, Lucille, Rebours, Vinciane, Reboux, Noemi, Rekik, Samia, Riachi, Ghassan, Rohmer, Barbara, Roquelaure, Bertrand, Rosa Hezode, Isabelle, Rostain, Florian, Saurin, Jean-Christophe, Servais, Laure, Stan-Iuga, Roxana, Subtil, Clément, Texier, Charles, Thomassin, Lucie, Tougeron, David, Tsakiris, Laurent, Valats, Jean-Christophe, Vuitton, Lucine, Wallenhorst, Timothée, Wangerme, Marc, Zanaldi, Hélène, Zerbib, Frank, Bai, Chen-Guang, Bian, Yun, Cai, Zhen-Zhai, Chang, Xiao-Yan, Chen, Guo-Dong, Cheng, Li, Chen, Yu, Guo, Jin-Tao, Guo, Tao, Han, Jun-Ling, He, Chao-Hui, Hu, Liang-Hao, Huang, Hao-Jie, Huang, Li, Huang, Li-Ya, Huang, Si-Lin, Huang, Wei, Jiang, Fei, Jiang, Hui, Lu, Feng-Chun, Lu, Guo-Tao, Lu, Zi-Peng, Li, Hui-Ping, Li, Jing, Li, Le, Li, Qiang, Li, Xiao-Yu, Lin, Qing, Lin, Yu-Li, Liu, Gai-Fang, Liu, Jie-Min, Liu, Li-Xin, Liu, Pi, Liu, Yi-Pin, Lu, Dong, Shao, Xiao-Dong, Shao, Zhuo, Song, Xu-Rui, Wang, Lei, Wang, Li-Juan, Wang, Li-Sheng, Wang, Lin, Wang, Wei, Wang, Zheng, Wen, Li, Wu, Xi, Xin, Lei, Xue, Jing, Yang, Hong, Yang, Jian-Feng, Yin, Tao, Zhang, Bei-Ping, Zhang, Guo-Wei, Zhang, Hong, Zhang, Rong-Chun, Zhao, Yi-Jun, Zhou, Si-Si, Zhu, Ke-Xiang, Masson, Emmanuelle, Zou, Wen-Bin, Pu, Na, Génin, Emmanuelle, Wu, Hao, Lin, Jin-Huan, Wang, Yuan-Chen, Li, Zhao-Shen, Cooper, David N., Férec, Claude, Liao, Zhuan, and Chen, Jian-Min

Published
2023
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17. Nanoparticles Binding to Lipid Membranes: from Vesicle-Based Gels to Vesicle Inversion and Destruction

Author

Wood, Derek A., Zuraw-Weston, Sarah, Torres, Ian K., Lee, YiWei, Wang, Li-Sheng, Jiang, Ziwen, Lázaro, Guillermo R., Wang, ShiYu, Rodal, Avital A., Hagan, Michael F., Rotello, Vincent M., and Dinsmore, Anthony D.

Subjects
Condensed Matter - Soft Condensed Matter
Abstract

Cells offer numerous inspiring examples where proteins and membranes combine to form complex structures that are key to intracellular compartmentalization, cargo transport, and specialization of cell morphology. Despite this wealth of examples, we still lack the design principles to control membrane morphology in synthetic systems. Here we show that even the relatively simple case of spherical nanoparticles binding to lipid-bilayer membrane vesicles results in a remarkably rich set of morphologies that can be controlled quantitatively via the particle binding energy. We find that when the binding energy is weak relative to a characteristic membrane-bending energy, the vesicles adhere to one another and form a soft solid, which could be used as a useful platform for controlled release. When the binding energy is larger, the vesicles undergo a remarkable destruction process consisting first of invaginated tubules, followed by vesicles turning inside-out, yielding a network of nanoparticle-membrane tubules. We propose that the crossover from one behavior to the other is triggered by the transition from partial to complete wrapping of nanoparticles. This model is confirmed by computer simulations and by quantitative estimates of the binding energy. These findings open the door to a new class of vesicle-based, closed-cell gels that are more than 99% water and can encapsulate and release on demand. Our results also show how to intentionally drive dramatic shape changes in vesicles as a step toward shape-responsive particles. Finally, they help us to unify the wide range of previously observed responses of vesicles and cells to added nanoparticles., Comment: 13 pages, 8 figures, and supplemental materials

Published
2018

21. Advances in the self-organized total synthesis of natural products.

Author

Wang, Li-Sheng, Xiang, Jia-Chen, and Wu, An-Xin

Subjects
*NATURAL products, *ORGANIC synthesis, *BIOMASS chemicals, *SERENDIPITY, *RESEARCH teams
Abstract

Natural product total synthesis has trailblazed in the era of multistep synthesis. The strategic application of existing synthetic methodologies and the stepwise construction that revolves around newly developed, tailored key steps, are two basic tactics in the principle of classic retrosynthetic analysis. However, a new synthetic model, termed self-organized total synthesis, has emerged in recent years, enabling the rapid creation of specific natural products by a one-pot reaction. Distinct from conventional analysis associated with certain bond disconnections, the design of self-organized total synthesis focuses on seeking a series of self-organized reaction sequences which can be integrated compatibly under a uniform condition, therefore allowing the entire sequence to proceed in one pot, and most importantly, starting from commercially available feedstocks or biomass materials. Whilst dauntingly challenging, this synthetic strategy is more consistent with the biogenetic pathway of natural products compared with conventional counterparts, and will hopefully provide the shortest synthesis for such natural products. Through this rational analysis, one-pot total synthesis is no longer in the way of serendipity but can be precisely designed and manipulated. In this review, we account for the definition, delimitation, and categorization of self-organized total synthesis and then elucidate a comprehensive understanding of this synthetic strategy based on our intensive explorations. We also highlight the contributions of other research groups in this growing field and anticipate that it will give rise to advancing new methodologies, as well as new concepts within organic synthesis. [ABSTRACT FROM AUTHOR]

Published
2024
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24. I2–DMSO‐Mediated Multicomponent [2+1+1+1] Annulation Reaction via Ethyl Nitroacetate C—NO2 Bond Cleavage as a C1 Synthon: A Route to Multisubstituted β‐Pyrrolidinones Derivatives with a Quaternary Center.

Author

Yu, Zhi‐Cheng, Shen, Xi, Zhou, You, Chen, Xiang‐Long, Wang, Li‐Sheng, Wu, Yan‐Dong, and Wu, An‐Xin

Abstract

Comprehensive Summary: A [2 + 1 + 1 + 1] cyclization reaction has been developed for the synthesis of multisubstituted β‐pyrrolidinones from commercially available aryl methyl ketones, primary amines, and ethyl nitroacetate. In this I2–DMSO‐meditated process, the C—NO2 bond of ethyl nitroacetate is cleaved, affording a C1 synthon, and the formation of two C—C and two C—N bonds and a quaternary carbon center are constructed in one pot. This method has good substrate compatibility and permits the late‐stage modification of pharmaceutical compounds. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Mineral Composition and Genesis of Black Quartzite Jade from Linwu County, Hunan Province

Author

MIAO Xu, SHI Miao, and WANG Li-sheng

Subjects
quartzite jade, mineral composition, mineralization, polarizing microscope, x-ray powder diffraction, x-ray fluorescence spectrometry, inductively coupled plasma-mass spectrometry, Geology, QE1-996.5, Ecology, QH540-549.5
Abstract

BACKGROUND Black quartzite jade is a type of commonly and commercially available jade. Many areas produce this gem. OBJECTIVES To analyze the gemological characteristics and mineral composition of the jade samples and to discuss their genesis. METHODS Conventional gemological tests, infrared spectrum tests, polarizing microscopy, X-ray powder diffraction analysis, X-ray fluorescence spectrometry, and inductively coupled plasma mass spectrometry were used. RESULTS Black quartzite jade from the Linwu County, Hunan Province, showed a medium-fine grain texture. The polarizing microscopy results revealed many crytalloblastic or palimpsest textures. The content of the main mineral quartz was 44.7%, the content of secondary minerals (mica and feldspar) was 31.0%, and the content of clay minerals was 12.7%. Minor organic carbon, iron minerals, andalusite, almandine, and pyrite were also observed. CONCLUSIONS Based on the main textures, structures, configuration features, and chemical compositions, the studied samples are regarded as typical low-to-moderate temperature hydrothermal regional metamorphic rocks, which are classified as greenschist facies. The source rock was composed of sedimentary rocks abundant with aluminum, quartz, and feldspar. The tectonic environment for its formation belonged to the continental margin. This study provides technical support for the identification of the mineral composition of quartzite jades from the Linwu County, Hunan Province.

Published
2021
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32. Difluorocarbene-Enabled Trifluoromethylation and Cyclization for the Synthesis of 3-(Trifluoromethyl)-4H-pyrans

Author

Wu, Chun-Yan, Chen, Xiang-Long, Yang, Dong-Sheng, Tang, Yong-Xing, Wang, Li-Sheng, Du, Yong-Dong, Wu, Yan-Dong, and Wu, An-Xin

Abstract

A three-component annulation reaction and trifluoromethylation for the construction of 3-(trifluoromethyl)-4H-pyrans using β-CF3-1,3-enynes, BrCF2CO2Et, and sulfoxonium ylides as readily available substrates has been developed. This metal-free process involves two C–F bond cleavages of β-CF3-1,3-enynes and a CF3group generated in situfrom BrCF2CO2Et. This method is applicable to the late-stage modification of pharmaceutically active molecules.

Published
2024
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33. I2-Induced Umpolung: Synthesis of a 1,6-Dihydrofuro[3,2-b]pyrazolo[3,4-e][1,4]thiazine Skeleton via an Unconventional 1,4-Dithiane-2,5-diol Reaction Mode

Author

Yu, Zhi-Cheng, Zheng, Kai-Lu, Shen, Xi, Zhou, You, Chen, Xiang-Long, Wang, Li-Sheng, Wu, Yan-Dong, and Wu, An-Xin

Abstract

In this paper, novel sulfur-containing 1,6-dihydrofuro[3,2-b]pyrazolo[3,4-e][1,4]thiazine skeletons were constructed from the simple and readily available materials enaminone, 5-aminopyrazole, and 1,4-dithiane-2,5-diol. Furthermore, a novel 1,4-dithiane-2,5-diol reaction mode has been developed through a double-dipole-reversal process induced by iodine that results in the formation of six new bonds and two new rings in a one-pot reaction. This method shows good substrate compatibility, and the products can be further modified with a variety of pharmaceuticals. Additionally, this novel skeleton exhibits good fluorescence properties in solution, enabling bright and stable green fluorescence imaging in HeLa cells.

Published
2024
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34. N-Benzylhydroxylamine as a novel synthetic block in "C1N1" embedding reaction via α-C(sp3)–H activation strategy.

Author

Tang, Yong-Xing, Zhou, You, Wu, Hao-Xuan, Wang, Li-Sheng, Wu, Chun-Yan, Zhuang, Shi-Yi, and Wu, An-Xin

Subjects
AROMATIC amines, RING formation (Chemistry), MOLECULES
Abstract

A novel process using N-benzylhydroxylamine hydrochloride as a "C1N1 synthon" in [2+2+1] cyclization for the construction of 1,2,5-trisubstituted imidazoles has been described for the first time. The key to realizing this process lies in capturing arylamines by in situ generated novel acyl ketonitrone intermediates. Subsequent tautomerization activates the α-C(sp3)–H of N-benzylhydroxylamines, and thus breaks through its inherent reaction mode and achieves N, α-C site-selective cyclization. Furthermore, this method enables scale-up synthesis and late-stage modification of complex molecules. [ABSTRACT FROM AUTHOR]

Published
2024
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38. Structural Design of Electric Steering Gear Transmission Device of Aircraft

Author

Wang Li Sheng Zhixiao Cao Lei

Subjects
Electric steering gear, Mechanical engineering and machinery, TJ1-1570
Abstract

The cylindrical gears are used to drive the traditional electric steering gear. There are many problems such as heavy weight，complex space structure and more transmission series，so the transmission efficiency is low. In view of the above problems，harmonic gear transmission is used instead of cylindrical gear transmission to determine the overall scheme，component structure. The Creo software is used to build the three-dimensional solid model of the transmission device，and the trial-manufacture of the principle prototype is completed after processing，manufacturing， installation and commissioning. The study results show that the scheme of applying harmonic gear transmission on electric steering gear transmission device is feasible，a foundation for theoretical and experimental research of optimizing electric steering gear transmission is laid.

Published
2020
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43. Mesenchymal Stem Cells Ameliorate DSS-Induced Experimental Colitis by Modulating the Gut Microbiota and MUC-1 Pathway

Author

Wang,Han, Sun,Yang, Xiao,Feng-Jun, Zhao,Xia, Zhang,Wei-Yuan, Xia,Yu-Jun, and Wang,Li-Sheng

Subjects
Immunology, Immunology and Allergy, Journal of Inflammation Research
Abstract

Han Wang,1,2,* Yang Sun,1,2,* Feng-Jun Xiao,3,* Xia Zhao,2,4 Wei-Yuan Zhang,1,2 Yu-Jun Xia,1 Li-Sheng Wang1,2 1School of Basic Medicine, Qingdao University, Qingdao, 266071, People’s Republic of China; 2Laboratory of Molecular Diagnosis and Regenerative Medicine, The Affiliated Hospital of Qingdao University, Qingdao, 266000, People’s Republic of China; 3Beijing Institute of Radiation Medicine, Beijing, 100850, People’s Republic of China; 4Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao, 266000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Li-Sheng Wang, Laboratory of Molecular Diagnosis and Regenerative Medicine, The Affiliated Hospital of Qingdao University, Qingdao, 266000, People’s Republic of China, Email lishengwang@qdu.edu.cn Yu-Jun Xia, School of Basic Medicine, Qingdao University, Qingdao, 266071, People’s Republic of China, Email xiayujun62@163.comPurpose: Mesenchymal stem cells (MSCs) have become novel therapeutic agents for the treatment of inflammatory bowel diseases (IBDs). However, the precise cellular and molecular mechanisms by which MSCs restore intestinal tissue homeostasis and repair the epithelial barrier have not been well elucidated. This study aimed to investigate the therapeutic effects and possible mechanisms of human MSCs in the treatment of experimental colitis.Methods: We performed an integrative transcriptomic, proteomic, untargeted metabolomics, and gut microbiota analyses in a dextran sulfate sodium (DSS)-induced IBD mouse model. The cell viability of IEC-6 cells was determined by Cell Counting Kit-8 (CCK-8) assay. The expression of MUC-1 and ferroptosis-related genes were determined by immunohistochemical staining, Western blot, and real-time quantitative polymerase chain reaction (RT-qPCR).Results: Mice treated with MSCs showed notable amelioration in the severity of DSS-induced colitis, which was associated with reduced levels of proinflammatory cytokines and restoration of the lymphocyte subpopulation balance. Treatment with MSC restored the gut microbiota and altered their metabolites in DSS-induced IBD mice. The 16s rDNA sequencing showed that treatment with MSC modulated the composition of probiotics, including the upregulation of the contents of Firmicutes, Lactobacillus, Blautia, Clostridia, and Helicobacter bacteria in mouse colons. Protein proteomics and transcriptome analyses revealed that pathways related to cell immune responses, including inflammatory cytokines, were suppressed in the MSC group. The ferroptosis-related gene, MUC-1, was significantly upregulated in the MSC-treated group. MUC-1-inhibition experiments indicated that MUC-1 was essential for epithelial cell growth. Through overexpression of MUC-1, it showed that upregulation of SLC7A11 and GPX4, and downregulation of ACSL4 in erastin and RSL3-treated IEC-6 cells, respectively.Conclusion: This study described a mechanism by which treatment with MSCs ameliorated the severity of DSS-induced colitis by modulating the gut microbiota, immune response, and the MUC-1 pathway.Keywords: inflammatory bowel disease, mesenchymal stem cells, gut microbiota, MUC-1, multi-omics

Published
2023

47. Classification of PRSS1 variants responsible for chronic pancreatitis: An expert perspective from the Franco-Chinese GREPAN Study Group

Author

Masson, Emmanuelle, primary, Zou, Wen-Bin, additional, Pu, Na, additional, Rebours, Vinciane, additional, Génin, Emmanuelle, additional, Wu, Hao, additional, Lin, Jin-Huan, additional, Wang, Yuan-Chen, additional, Li, Zhao-Shen, additional, Cooper, David N., additional, Férec, Claude, additional, Liao, Zhuan, additional, Chen, Jian-Min, additional, Abrantes, Amandine, additional, Aguilera Munoz, Lina, additional, Albouys, Jérémie, additional, Alric, Laurent, additional, Amiot, Xavier, additional, Archambeaud, Isabelle, additional, Audiau, Solène, additional, Bastide, Laetitia, additional, Baudon, Julien, additional, Bellaiche, Guy, additional, Bellon, Serge, additional, Bertrand, Valérie, additional, Bideau, Karine, additional, Billiemaz, Kareen, additional, Billioud, Claire, additional, Bonnefoy, Sabine, additional, Borderon, Corinne, additional, Bournet, Barbara, additional, Breton, Estelle, additional, Brugel, Mathias, additional, Buscail, Louis, additional, Cadiot, Guillaume, additional, Camus, Marine, additional, Causse, Xavier, additional, Chamouard, Patrick, additional, Chaput, Ulriikka, additional, Cholet, Franck, additional, Ciocan, Dragos Marius, additional, Clavel, Christine, additional, Coffin, Benoit, additional, Coimet-Berger, Laura, additional, Creveaux, Isabelle, additional, Culetto, Adrian, additional, Daboussi, Oussama, additional, De Mestier, Louis, additional, Degand, Thibault, additional, D'Engremont, Christelle, additional, Denis, Bernard, additional, Dermine, Solène, additional, Desgrippes, Romain, additional, Drouet D'Aubigny, Augustin, additional, Enaud, Raphaël, additional, Fabre, Alexandre, additional, Gargot, Dany, additional, Gelsi, Eve, additional, Gentilcore, Elena, additional, Gincul, Rodica, additional, Ginglinger-Favre, Emmanuelle, additional, Giovannini, Marc, additional, Gomercic, Cécile, additional, Gondran, Hannah, additional, Grainville, Thomas, additional, Grandval, Philippe, additional, Grasset, Denis, additional, Grimaldi, Stéphane, additional, Grimbert, Sylvie, additional, Hagege, Hervé, additional, Heissat, Sophie, additional, Hentic, Olivia, additional, Herber-Mayne, Anne, additional, Hervouet, Marc, additional, Hoibian, Solene, additional, Jacques, Jérémie, additional, Jais, Bénédicte, additional, Kaassis, Mehdi, additional, Koch, Stéphane, additional, Lacaze, Elodie, additional, Lacroute, Joël, additional, Lamireau, Thierry, additional, Laurent, Lucie, additional, Le Guillou, Xavier, additional, Le Rhun, Marc, additional, Leblanc, Sarah, additional, Levy, Philippe, additional, Lievre, Astrid, additional, Lorenzo, Diane, additional, Maire, Frédérique, additional, Marcel, Kévin, additional, Matias, Clément, additional, Mauillon, Jacques, additional, Morgant, Stéphanie, additional, Moussata, Driffa, additional, Muller, Nelly, additional, Nambot, Sophie, additional, Napoleon, Bertrand, additional, Olivier, Anne, additional, Pagenault, Maël, additional, Pelletier, Anne-laure, additional, Pennec, Olivier, additional, Pinard, Fabien, additional, Pioche, Mathieu, additional, Prost, Bénédicte, additional, Queneherve, Lucille, additional, Reboux, Noemi, additional, Rekik, Samia, additional, Riachi, Ghassan, additional, Rohmer, Barbara, additional, Roquelaure, Bertrand, additional, Rosa Hezode, Isabelle, additional, Rostain, Florian, additional, Saurin, Jean-Christophe, additional, Servais, Laure, additional, Stan-Iuga, Roxana, additional, Subtil, Clément, additional, Texier, Charles, additional, Thomassin, Lucie, additional, Tougeron, David, additional, Tsakiris, Laurent, additional, Valats, Jean-Christophe, additional, Vuitton, Lucine, additional, Wallenhorst, Timothée, additional, Wangerme, Marc, additional, Zanaldi, Hélène, additional, Zerbib, Frank, additional, Bai, Chen-Guang, additional, Bian, Yun, additional, Cai, Zhen-Zhai, additional, Chang, Xiao-Yan, additional, Chen, Guo-Dong, additional, Cheng, Li, additional, Chen, Yu, additional, Guo, Jin-Tao, additional, Guo, Tao, additional, Han, Jun-Ling, additional, He, Chao-Hui, additional, Hu, Liang-Hao, additional, Huang, Hao-Jie, additional, Huang, Li, additional, Huang, Li-Ya, additional, Huang, Si-Lin, additional, Huang, Wei, additional, Jiang, Fei, additional, Jiang, Hui, additional, Lu, Feng-Chun, additional, Lu, Guo-Tao, additional, Lu, Zi-Peng, additional, Li, Hui-Ping, additional, Li, Jing, additional, Li, Le, additional, Li, Qiang, additional, Li, Xiao-Yu, additional, Lin, Qing, additional, Lin, Yu-Li, additional, Liu, Gai-Fang, additional, Liu, Jie-Min, additional, Liu, Li-Xin, additional, Liu, Pi, additional, Liu, Yi-Pin, additional, Lu, Dong, additional, Shao, Xiao-Dong, additional, Shao, Zhuo, additional, Song, Xu-Rui, additional, Wang, Lei, additional, Wang, Li-Juan, additional, Wang, Li-Sheng, additional, Wang, Lin, additional, Wang, Wei, additional, Wang, Zheng, additional, Wen, Li, additional, Wu, Xi, additional, Xin, Lei, additional, Xue, Jing, additional, Yang, Hong, additional, Yang, Jian-Feng, additional, Yin, Tao, additional, Zhang, Bei-Ping, additional, Zhang, Guo-Wei, additional, Zhang, Hong, additional, Zhang, Rong-Chun, additional, Zhao, Yi-Jun, additional, Zhou, Si-Si, additional, and Zhu, Ke-Xiang, additional

Published
2023
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