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The unfolded protein response ( UPR) plays important roles in plant development and plant-pathogen interactions, as well as in plant adaptation to adverse environmental stresses. Previously b ZIP28 and b ZIP60 have been identified as important UPR regulators for mitigating the endoplasmic reticulum ( ER) stress in Arabidopsis thaliana. Here we report the biological function of NAC103 in a novel transcriptional regulatory cascade, connecting b ZIP60 to the UPR downstream genes in Arabidopsis. Expression of NAC103 was induced by ER stress, and was completely abolished in the b ZIP60 null mutant. A new ER stress-responsive cis-element UPRE- III ( TCATCG) on the NAC103 promoter was identified, and trans-activation of UPRE- III by b ZIP60 was confirmed in both yeast cells and Arabidopsis protoplasts. The direct binding of b ZIP60 to UPRE- III-containing DNA was also demonstrated in an electrophoretic mobility shift assay. NAC103 formed homodimers in yeast two-hybrid and bimolecular fluorescence complementation assays. It had transcriptional activation activity and was localized in the nucleus. Over-expression of NAC103 had pleiotropic effects on plant growth, and induced expression of several UPR downstream genes in Arabidopsis under normal growth conditions. The activation of UPR gene promoters by NAC103 was also confirmed in effector/reporter protoplast assays. Thus, our study demonstrates a transcriptional regulatory cascade in which NAC103 relays ER stress signals from b ZIP60 to UPR downstream genes through a newly identified ER stress cis-element ( UPRE- III) and transcriptional activation activity of its encoded protein NAC103. [ABSTRACT FROM AUTHOR]

Sewage sludge was amended with calcium-based bentonite with addition of no more than 10% in dry weight during the 52-day aerobic composting process, the variations of temperature, pH, organic carbon, EC, total nitrogen, nitrate(NH 4 + -N) and ammonium(NO 3 - -N) were investigated, as well as the compost detoxification(germination test), heavy metals(Zn, Cu, Pb, Cd) passivation and estrone(E1) elimination. The results showed that the amendment facilitated the thermophilic phase, promoted the compost heat inactivation and brought the organic carbon mineral up to more than 15.27% -19.71%. During the composting, the compost pH increased at the beginning and then gradually decreased before reaching values of 6.76-7.05, while the amendments alleviated the dramatic pH value fluctuation. The bentonite amendment reduced the salinity of the compost with final product EC remarkably lower than 1132 μS·cm -1 of the control treatment, and the effect was enhanced with the increase of addition amount. The total nitrogen content increased with time, and there was a remarkable ammonia loss in the beginning stage for the control treatment, while the bentonite addition could facilitate the total nitrogen content increase by reducing the ammonia loss. With the composting variation, the contents of NH 4 + -N increased and then decreased while the NO 3 - -N content increased gradually. The bentonite addition had a slight inhibitory effect on the plant germination but did not influence the compost maturity and detoxification; meanwhile, the amendment improved the heavy metal passivation and reduced the E1 content, especially from 90.48 to 28.27 μg·kg -1 with 5% treatment during the composting. The study indicated that bentonite addition of lower than 5% was acceptable for the sludge compost amendment, which had great potential in sludge hygienization, detoxification, heavy metal passivation and E1 elimination.

Aiming to evaluate the influence of alkali solids amendment on the sewage sludge aerobic composting and to declare the potential of related composts on infertile soil amelioration, sewage sludge and sawdust mixture were amended with straw biochar, hardwood biochar, fly ash and lime by 10% addition ratio in dry weight during the aerobic composting process; finally, potential of the related composts on the infertile soil amelioration was investigated by pot experiment. The results showed that the alkali solids amendment could prolong the thermophilic phase, and promote the composting material heat inactivation. Addition of straw and hardwood biochar facilitated more than 21.65% and 18.16% organic matter degradation. During the composting, the compost pH decreased at the beginning and then gradually increased before reached values of 6.78-7.33, while the fly ash and lime amendments could lead to higher pH values in the beginning stage. The alkali solids amendment reduced the salinity of the compost with final products EC lower than 3000 μS·cm -1 . The addition of straw and hardwood biochar could increase the total nitrogen content by reducing the ammonia loss at the beginning, while the fly ash and lime amendments would result in ammonia loss in the beginning stage. Despite the composting variation, the contents of nitrate increased and the ammonium salt content decreased gradually. The biochar addition can accelerate the nitrate transformation while the fly ash and lime amendment had slight inhibitory effect on the nitrate transformation and plant germination. The pot experiment revealed that applying compost products could significantly improve the brassica growth, Cu and Zn micronutrients accumulation since the compost addition could increase the soil organic carbon content, as well as soil N, P, and K contents. Compared with the non-amended compost, the alkali solids amendment could slightly reduce the Cu and Zn micronutrients accumulation, while the fertile potentials were acceptable. The study indicated that straw and hardwood biochar were more suitable than fly ash and lime as compost amendment, and the related compost products had great potential on infertile soil amelioration.