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It is still a serious challenge to develop a simple synthesized method of SAPO‐34 sieve modified by Cu with high NH3‐SCR performance. The Cu/SAPO‐34 samples with hierarchical structure were synthesized for the first time using cupric citrate as copper source by one‐pot hydrothermal method which showed excellent NH3‐SCR performance for NO removal. Among them, the 0.15Cu/SAPO‐34 catalyst with a Cu/Al ratio of 0.15 exhibited the widest temperature operating window (160–500 °C) and better hydrothermal stability. The physicochemical properties of 0.15Cu/SAPO‐34 was measured to understand the impacts of the Cu species on the NH3‐SCR of NO activity by various characterization methods including XRD, NH3‐TPD, N2 adsorption‐desorption, XPS, EPR, UV‐vis, NH3‐oxidation, NMR and in situ DRIFTS. The results reveal that the good activity is assigned to the isolated Cu species located at the site (I) in SAPO‐34, and the co‐existence of surface Cu2+ and Cu+ species with almost equal amounts. In addition, 0.15Cu/SAPO‐34 also possesses suitable acidic sites which are conducive to catalytic activity. The results of in situ DRIFTS suggest that the NH3‐SCR reaction follows Eley‐Rideal mechanism over 0.15Cu/SAPO‐34. [ABSTRACT FROM AUTHOR]

Summary: The plant flavonoid dogma proposes that labile plant flavonoid carbocations (PFCs) play vital roles in the biosynthesis of proanthocyanidins (PAs). However, whether PFCs exist in plants and how PFCs function remain unclear. Here, we report the use of an integrative strategy including enzymatic assays, mutant analysis, metabolic engineering, isotope labeling and metabolic profiling to capture PFCs and demonstrate their functions. In anthocyanidin reductase (ANR) assays, an (−)‐epicatechin conjugate was captured in protic polar nucleophilic methanol alone or methanol−HCl extracts. Tandem mass spectrum (MS/MS) analysis characterized this compound as an (−)‐epicatechin‐4‐O‐methyl (EOM) ether, which resulted from (−)‐epicatechin carbocation and the methyl group of methanol. Acid‐based catalysis of procyanidin B2 and B3 produced four compounds, which were annotated as two EOM and two (+)‐catechin‐4‐O‐methyl (COM) ethers. Metabolic profiling of seven PA pathway mutants showed an absence or reduction of two EOM ether isomers in seeds. Camellia sinensis ANRa (CsANRa), leucoanthocyanidin reductase c (CsLARc), and CsMYB5b (a transcription factor) were independently overexpressed for successful PA engineering in tobacco. The EOM ether was remarkably increased in CsANRa and CsMYB5b transgenic flowers. Further metabolic profiling for eight green tea tissues revealed two EOM and two COM ethers associated with PA biosynthesis. Moreover, an incubation of (−)‐epicatechin or (+)‐catechin with epicatechin carbocation in CsANRa transgenic flower extracts formed dimeric procyanidin B1 or B2, demonstrating the role of flavan‐3‐ol carbocation in the formation of PAs. Taken together, these findings indicated that flavan‐3‐ol carbocations exist in extracts and are involved in the biosynthesis of PAs of plants. Significance Statement: Although the dogma of plant flavonoids proposes that labile plant flavonoids carbocations (PFCs) exist to associate with the biosynthesis of proanthocyanidins (PAs), supportive evidence is lacking. Data from biochemical analysis, isotope labeling, genetic analysis, metabolic engineering and metabolic profiling demonstrate that PFCs are involved in the biosynthesis of PAs. [ABSTRACT FROM AUTHOR]

A short and efficient synthesis of (+)-subersic acid in 6 steps with an overall yield of 8.6% from (−)-Sclareol was presented using Mitsunobu reaction and Claisen rearrangement as critical steps. This elegant protocol gave a convenient access to gram scales of (+)-subersic acid, which could be a precursor for its structural analogues and for further bioactivity researches. [ABSTRACT FROM AUTHOR]

This paper describes a concise and practical route to (+)-subersic acid from naturally occurring (−)-Sclareol in 7 steps. The key step of this route relied on the cross coupling of the diterpene with the arene fragment using the arylation of allylic acetate followed by β-acetoxy elimination type Heck reaction. [ABSTRACT FROM AUTHOR]

A new method has been developed for the copper-mediated trifluoromethylthiolation of allylic halides by using potassium fluoride, elemental sulfur, and (trifluoromethyl)trimethylsilane in anhydrous N, N-dimethylformamide. This protocol provides facile access to a variety of allylic trifluoromethyl thioethers in moderate to good yields under mild, ligand-free reaction conditions. [ABSTRACT FROM AUTHOR]

Background: The tea plant (Camellia sinensis (L.) O. Kuntze) is one of the most economically important woody crops. Plastic greenhouse covering cultivation has been widely used in tea areas of northern China. Chlorophyll is not only the crucial pigment for green tea, but also plays an important role in the growth and development of tea plants. Currently, little is known about the effect of plastic greenhouse covering cultivation on chlorophyll in tea leaves. Results: To investigate the effect of plastic greenhouse covering cultivation on chlorophyll in tea leaves, color difference values, chlorophyll contents, gene expression, enzyme activities and photosynthetic parameters were analyzed in our study. Sensory evaluation showed the color of appearance, liquor and infused leaves of greenhouse tea was greener than field tea. Color difference analysis for tea liquor revealed that the value of ∆L, ∆b and b/a of greenhouse tea was significantly higher than field tea. Significant increase in chlorophyll content, intracellular CO2, stomatal conductance, transpiration rate, and net photosynthetic rate was observed in greenhouse tea leaves. The gene expression and activities of chlorophyll-metabolism-related enzymes in tea leaves were also activated by greenhouse covering. Conclusion: The higher contents of chlorophyll a, chlorophyll b and total chlorophyll in greenhouse tea samples were primarily due to higher gene expression and activities of chlorophyll-metabolism-related enzymes especially, chlorophyll a synthetase (chlG), pheophorbide a oxygenase (PAO) and chlorophyllide a oxygenase (CAO) in tea leaves covered by greenhouse. In general, our results revealed the molecular basis of chlorophyll metabolism in tea leaves caused by plastic greenhouse covering cultivation, which had great significance in production of greenhouse tea. [ABSTRACT FROM AUTHOR]

Cu ion-exchanged SAPO-34 with hierarchical pore structure (Cu/SAPO-34-H) presents excellent NH 3 -SCR activity, which is attributed to the active Cu species located in D6R. • Preparation of Cu/SAPO-34-H with hierarchical pore structure by ion exchanged method. • High NH 3 -SCR of NO x activity over 2.7 wt%Cu/SAPO-34-H at 170–480 °C even under 10%H 2 O. • Excellent SCR activity due to Cu2+ mainly located in D6R in Cu/SAPO-34-H. • Hierarchical pore structure promote the generation stability and of active Cu species. • Much better hydrothermal stability at either high temperature or low temperature. SAPO-34 with hierarchical pore structure (SAPO-34-H) was first prepared by a hard-template method using CaCO 3 as template. The x Cu/SAPO-34-H (x = 1.1 wt%, 1.9 wt%, 2.7 wt% and 3.2 wt%) catalysts were synthesized by ion-exchanged method and applied for selective catalytic reduction (SCR) of NOx with NH 3. XRD, XRF, SEM, TEM, UV–vis-DRS, NH 3 -TPD, XPS, NMR, EPR and in situ DRIFTS were used to characterize the physiochemical properties of the catalysts. The SCR activity results demonstrate that 100% NOx conversion can be achieved over the 2.7 wt%Cu/SAPO-34-H catalyst at about 150 °C in the GHSV of 40,000 h−1. Furthermore, NOx conversion under the testing conditions of H 2 O and SO 2 was slightly influenced over the 2.7 wt%Cu/SAPO-34-H catalyst after high-temperature hydrothermal aging at 700 and 800 °C, respectively. NOx convention presents above 90% at 170–480 °C even introducing 10% H 2 O for 10 h, and it can be maintained 90% by introducing 200 ppm SO 2 and 12% H 2 O over 2.7 wt%Cu/SAPO-34-H. In the meantime, 2.7 wt%Cu/SAPO-34-H was investigated through the low-temperature hydration treatment at 80 °C, it also displays superior hydration treatment resistance for low-temperature. The outstanding performance is ascribed to the hierarchical pore structure of 2.7 wt%Cu/SAPO-34-H, which can promote the diffusion of reaction gas and generation of active sites. EPR results indicate that the Cu2+ species mainly locates the site I in 2.7 wt%Cu/SAPO-34-H, while the Cu2+ species mainly locates the site III for the conversional 2.7 wt%Cu/SAPO-34. Moreover, the in situ DRIFTS results indicate that the Lewis acid sites are easy to participate in NH 3 -SCR reaction for 2.7 wt%Cu/SAPO-34-H, which mainly follows the Eley-Rideal (E-R) reaction mechanism at 200 °C for 2.7 wt%Cu/SAPO-34-H. [ABSTRACT FROM AUTHOR]

To save time and economic cost of the synthesis of SAPO-34 zeolites, the effects of hydrothermal time on the structure and methanol-to-olefin (MTO) catalytic activity of SAPO-34 zeolites were investigated in detail. A series of SAPO-34 zeolites (SAPO-34-x h, x = 3, 4, 8, 12, 24 and 48) were obtained using the traditional hydrothermal method by changing the synthesis time at 210 °C. The obtained catalysts were characterized by XRD, N2 adsorption–desorption, SEM, TG-DTA, ICP, 29Si and 27Al MAS NMR and NH3-TPD techniques. Among these samples, SAPO-34-4 h with a higher Si/(Si + Al + P) ratio shows a much longer lifetime (518 min) and higher light olefin selectivity (82.0%) in the MTO reaction, which can be attributed to the unique Si distribution and low acidity. Moreover, the SAPO-34-4 h sample can maintain good MTO performance even under long time storage without complicated processing or after several cycles of regeneration. This facile synthesis procedure may be beneficial for large scale industrial catalyst production because of its short synthesis time. [ABSTRACT FROM AUTHOR]

An abstract of the article "Concise Synthesis of (+)-Subersic Acid from (-)-Sclareol" by P. Wang and colleagues is presented.

A convenient process is described to convert a variety of allylic chlorides into allylic trifluoromethyl thioethers. [ABSTRACT FROM AUTHOR]

A new method has been developed for the copper(I)-mediated trifluoromethylthiolation of α-bromoketone using commercial anhydrous potassium fluoride, elemental sulfur, and (trifluoromethyl)-trimethylsilane in anhydrous N , N -dimethylformamide. This protocol provides facile access to a variety of α-trifluoromethylthiolated carbonyl compounds in moderate to excellent yields under mild and ligand free conditions. [ABSTRACT FROM AUTHOR]

Plants would encounter various biotic and abiotic stresses during the growth and development. WRKY transcription factors (TFs) as plant-specific TFs, play an important role in responding to various adverse circumstances. Despite some advances were achieved in functional studies of WRKY TFs in tea plants, systematic analysis of the involvement of CsWRKY TFs when facing cold, salt, drought stresses and pathogen and insect attack was lacked. In present study, a total of 78 CsWRKY TFs were identified following the genomic and transcript databases. The expression patterns of CsWRKYs in various organs of tea plants and the expression profiles in response to biotic and abiotic stresses were investigated by examining representative RNA-seq data. Moreover, the effects of hormone treatments (SA and MeJA) on the transcription levels of WRKY TFs were also investigated. The phylogenetic tree of CsWRKY TFs from different species indicated the functional diversity of WRKY TFs was not closely related to their protein classification. Concurrently, CsWRKY70-2 TF was identified as a positive regulator in response to drought stress. This study provided solid and valuable information, helping us better understand the functional diversity of CsWRKY TFs, and laid the foundation for further research on the function of key WRKY genes in tea plants. In this study, 78 WRKY transcription factors had been identified in tea plants as accurately as possible based on the transcriptome and genomic data. At the transcriptomic level, the expression patterns of CsWRKYs in various organs of tea plants and the expression profiles in response to biotic (Ectropis oblique, tea leafhopper and gray-blight attack), abiotic stresses (Cold, drought, salt) and exogenous hormones (SA and MeJA) were investigated. Expression pattern analysis showed that most Cs WRKYs were involved in the process of tea plants resisting biotic and abiotic stresses. Phylogenetic analysis indicated that the functional diversity of CsWRKY TFs was not closely related to their protein classification. In addition, CsWRKY70-2 TF had been proved to play a positive regulatory role in drought stress tolerance. This study provided solid and valuable information, helping better understand the functional diversity of CsWRKY TFs, and laid the foundation for further research on the function of key WRKY genes in tea plants. [ABSTRACT FROM AUTHOR]

Drying is an important factor in the formation of green tea flavor. In this study, metabolomic analysis were used to detect characteristic components of four green teas with clean aroma (CA), chestnut-like aroma (CLA), bean-like aroma (BLA) and high-fired aroma (HFA) produced by adjusting drying temperature. The sensory evaluation results showed that the tea dried at 90°C, 110°C, 140°C and 160°C had a clean aroma, chestnut-like aroma, bean-like aroma and high-fired aroma respectively. A total of 95 volatile compounds were identified. Linalool, naphthalene, 2-methyl-naphthalene, 1-octen-3-ol contributed most to the clean aroma. 1,2-dihydro-1,1,6-trimethyl-naphthalene was the key component of the chestnut-like aroma. 2,3-diethyl-5-methyl-pyrazine, 2-methyl-butanal, 3-ethyl-2,5-dimethyl-pyrazine had the biggest contribution to the bean-like aroma. 2,3-diethyl-5-methyl-pyrazine, 3-ethyl-2,5-dimethyl-pyrazine and 2-ethyl-5-methyl-pyrazine contributed most to the high-fired aroma. A total of 838 non-volatile compounds were detected and amino acids, saccharides decreased while catechins, flavonols increased during the increasing of drying temperature. It's found that they were related to the taste of umami, astringency and bitterness. This result provides a guidance and theoretical basis for the processing of green tea with different aroma types. • Four aroma types of green teas were manufactured by different drying temperatures. • Naphthalene and its derivatives contributed most to clean and chestnut-like aroma. • Pyrazine derivatives contributed most to bean-like and high-fired aroma green teas. • The higher the drying temperature, the more bitter and more astringent of green tea. [ABSTRACT FROM AUTHOR]

In this study, gas chromatography–mass spectrometry (GC–MS) and gas chromatography–olfactometry (GC–O) analysis was performed to figure out the characteristic aroma compounds of high-latitude black teas and select the suitable cultivar for producing high-aroma black tea. Sensory evaluation revealed that Jinxuan presented an obviously floral aroma and got the highest score, followed by Longjing Changye, Zhongcha 302, Yingshuang, Baihaozao and Huangshan Quntizhong. Furthermore, a total of 135 volatiles were identified in all black teas and 12 components can be used to distinguish different latitude black teas by principal component analysis (PCA). Cluster analysis (CA) showed six samples were divided into two clusters and Jinxuan was classified separately of cluster B. Based on GC–O analysis, 41 volatile organic compounds (VOCs) were found to promote the aroma of high-latitude black teas. Among them, phenylethyl alcohol, geraniol, linalool, α-ionone, cis-3-hexenyl hexanoate and methyl salicylate were the major contributors to the floral fragrance of black tea and accounted for a relative high proportion in Jinxuan, Longjing Changye and Baihaozao. In summary, Jinxuan and Longjing Changye were suitable for producing high-quality black tea in high-latitude area. [ABSTRACT FROM AUTHOR]

Tea is one of the most widely consumed nonalcoholic beverages worldwide. Polyphenols are nutritional compounds present in the leaves of tea plants. Although numerous genes are functionally characterized to encode enzymes that catalyze the formation of diverse polyphenolic metabolites, transcriptional regulation of those different pathways such as late steps of the proanthcoyanidin (PA) pathway remains unclear. In this study, using different tea transcriptome databases, we screened at least 140 R2R3-MYB transcription factors (TFs) and grouped them according to the basic function domains of the R2R3 MYB TF superfamily. Among 140 R2R3 TFs, CsMYB5a and CsMYB5e were chosen for analysis because they may be involved in PA biosynthesis regulation. CsMYB5a- overexpressing tobacco plants exhibited downregulated anthocyanin accumulation but a high polymeric PA content in the flowers. Overexpression of CsMYB5e in tobacco plants did not change the anthocyanin content but increased the dimethylaminocinnamaldehyde-stained PA content. RNA-seq and qRT-PCR analyses revealed that genes related to PA and anthocyanin biosynthesis pathways were markedly upregulated in both CsMYB5a - and CsMYB5e -overexpressing flowers. Three UGTs and four GSTs were identified as involved in PA and anthocyanin glycosylation and transportation in transgenic plants. These results provide new insights into the regulation of PA and anthocyanin biosynthesis in Camellia sinensis . [ABSTRACT FROM AUTHOR]

[Display omitted] • Tar induced severe deactivation of SCR catalyst is first reported in China. • Proposed pyrolysis effectively removes the poisoning substances. • Heating rate is the critical factor to keep a superior mechanical property. • The generated catalyst show activity as high as the fresh catalyst. • Regeneration of SCR catalyst shows favorable environmental and economic benefits. Tar species on catalyst's surface promote the deposition of NH 4 HSO 4 /(NH 4) 2 SO 4 leading to the complete deactivation of V 2 O 5 -MoO 3 /TiO 2 catalyst utilized in a glass kiln. The proposed pyrolysis method in this work effectively removes the poisoning substances. TG-IR and TG-MS results indicate that the pyrolysis products are mainly CO 2 and SO 2 representing the pyrolysis of tar species and NH 4 HSO 4 /(NH 4) 2 SO 4. The heating rate and pyrolysis temperature are the critical factors to keep a superior mechanical property. The axial and transverse compressive strength remained 1.77 MPa and 0.53 Mpa falling only by 4.8% and 13.1% respectively through the optimized treatment at 420 °C for 2 h with heating rate of 3 K min−1. The characterizations indicated that the surface area, pore volume and acid property were apparently restored after the heat treatment. As a result, the regenerated powder catalyst showed 100% NO conversion and more than 94% N 2 selectivity at 250 – 300 °C and the regenerated honeycomb catalyst exhibited 91.6% NO conversion at 260 °C. The TPR and XPS results indicated changes of the redox behavior, which contracted the temperature range of excellent performance. Typically, the redox cycles shifting to high temperatures narrowed this temperature range, while the excessive oxidation ability decreased the N 2 selectivity. The schematic of tar induced deactivation and regeneration was proposed. Overall, the tar induced deactivated catalyst was successfully regenerated by proper pyrolysis and can be reutilized in the original glass kiln at 260 °C. [ABSTRACT FROM AUTHOR]

The 2-oxoglutarate-dependent dioxygenase (2-OGD) superfamily is one of the largest protein families in plants. The main oxidation reactions they catalyze in plants are hydroxylation, desaturation, demethylation, epimerization, and halogenation. Four members of the 2-OGD superfamily, i.e., flavonone 3β-hydroxylase (F3H), flavones synthase I (FNS I), flavonol synthase (FLS), and anthocyanidin synthase (ANS)/leucoanthocyanidin dioxygenase (LDOX), are present in the flavonoid pathway, catalyzing hydroxylation and desaturation reactions. In this review, we summarize the recent research progress on these proteins, from the discovery of their enzymatic activity, to their functional verification, to the analysis of the response they mediate in plants towards adversity. Substrate diversity analysis indicated that F3H, FNS Ⅰ, ANS/LDOX, and FLS perform their respective dominant functions in the flavonoid pathway, despite the presence of functional redundancy among them. The phylogenetic tree classified two types of FNS Ⅰ, one mainly performing FNS activity, and the other, a new type of FNS present in angiosperms, mainly involved in C-5 hydroxylation of SA. Additionally, a new class of LDOXs is highlighted, which can catalyze the conversion of (+)-catechin to cyanidin, further influencing the starter and extension unit composition of proanthocyanidins (PAs). The systematical description of the functional diversity and evolutionary relationship among these enzymes can facilitate the understanding of their impacts on plant metabolism. On the other hand, it provides molecular genetic evidence of the chemical evolution of flavonoids from lower to higher plants, promoting plant adaptation to harsh environments. [ABSTRACT FROM AUTHOR]

In the present work, a serial of Cu 0.02 Fe 0.2 Ce y Ti 1-y O x catalysts are prepared by sol-gel method and applied for NH 3 -SCR of NO, meanwhile Cu 0.02 Fe 0.2 Ce 0.2 Ti 0.8 O x shows good low-temperature NH 3 -SCR performance with/without water and an outstanding water resistance. The bulk structure, redox ability, surface acidity and surface species of Cu 0.02 Fe 0.2 Ce y Ti 1-y O x are measured and discussed by series of characterization in details to illuminate the reasons for the good low-temperature activity and water resistance. The Ce modification can tune the surface acidic distribution, improve the surface oxygen content and surface oxidation reduction cycle (Ce4+ + Fe2+ ↔ Ce3+ + Fe3+), which contribute the good activity. In addition, the effect of water on NH 3 -SCR performance over Cu 0.02 Fe 0.2 TiO x and Cu 0.02 Fe 0·2 Ce 0·2 Ti 0.8 O x are investigated emphatically by in situ DRIFTS. Image 1 • Excellent low-temperature activity for NH 3 -SCR of NO over Cu 0·02 Fe 0·2 Ce 0·2 Ti 0.8 O x. • Ce addition significantly improves the water tolerance for the NH 3 -SCR reaction. • Enhancement of surface acidity for Cu 0·02 Fe 0·2 Ce 0·2 Ti 0.8 O x due to the Ce addition. • Increase in Lewis acid sites contributes the good water tolerance. [ABSTRACT FROM AUTHOR]

• Improving low temperature SCR activity and resistance to H 2 O/SO 2 on Sm a MnNi 2 Ti 7 O x due to Sm addition. • Sm 0.3 MnNi 2 Ti 7 Ox shows over 90% NO conversion and N 2 selectivity in range of 140–329 °C. • The better SCR activity is attributed to the enhanced surface area, redox ability and acid amount for Sm 0.3 MnNi 2 Ti 7 Ox • The excellent activity and resistance to H 2 O/SO 2 of Sm 0.3 MnNi 2 Ti 7 O x is significant for its practical applications in NO removal A series of Sm-modified Sm a MnNi 2 Ti 7 O x (a = 0.1, 0.2, 0.3, 0.4) catalysts were synthesized and showed better NH 3 -SCR activity than MnNi 2 Ti 7 O x , meanwhile, the Sm 0.3 MnNi 2 Ti 7 O x sample exhibited much higher low temperature NH 3 -SCR performance below about 200 °C, and good SO 2 and/or H 2 O durability. The bulk and surface physicochemical properties were measured to investigate the influence of the Sm addition on the activity by various characterization methods including NO/NH 3 oxidation, NH 3 -TPD, H 2 -TPR, XRD, N 2 adsorption-desorption, XPS. The results illustrate that the Sm addition can not only increase the adsorbed NO/NH 3 species, BET surface area, surface acid amount, Mn4+ content, surface adsorbed oxygen and the redox ability but also inhibit the generation of ammonium sulfate / bisulfate, which are beneficial to the NH 3 -SCR activity. Moreover, the in situ diffuse reflectance infrared Fourier transform spectroscopy was applied to analyze the reaction process, which reveals that the NH 3 -SCR reaction follows both E–R and L-H mechanisms over Sm 0.3 MnNi 2 Ti 7 O x. [ABSTRACT FROM AUTHOR]

• Good activity in range of 210–420 °C with 100% NO conversion and N 2 selectivity. • The special activity due to more surface acid amount and suitable redox ability. • Monodentate nitrate and NH 2 species as main active intermediates. • H 2 O mainly restrains L-H reaction pathway leading to the deactivation of catalyst. A serial of novel Ni m Co 0.3-m Ti 0.7 O x (m = 0.05-0.25) mixed-oxide catalysts was synthesized by sol-gel method, and applied for selective catalytic reduction (SCR) of NO x by NH 3. Influences of the Ni/Co ratio on the physi co- chemical properties and SCR performance were characterized by various analytical techniques, including BET surface area, XRD, XPS, NH 3 -TPD, H 2 -TPR and in situ DRIFTS. The Ni 0.1 Co 0.2 Ti 0.7 O x catalyst shows optimum NH 3 -SCR activity and excellent N 2 selectivity with a broader activity temperature window (210–450 °C), which is associated with the much higher BET surface area, more surface acid amounts and the suitable redox ability derived from the strong interaction between Co and Ni (Co3+ + Ni2+ ↔ Ni3+ + Co2+). In situ DRIFTS results reveal that the formed monodentate nitrate and NH 2 species, as main active intermediates, are favor of the NH 3 -SCR reaction. The existence of H 2 O mainly restrains Langmuir-Hinshelwood reaction pathway which may be the main cause for the catalyst deactivation. [ABSTRACT FROM AUTHOR]

Flavan-3-ols and oligomeric proanthocyanidins (PAs) are the main nutritional polyphenols in green tea (Camellia sinensis), which provide numerous benefits to human health. To date, the regulatory mechanism of flavan-3-ol biosynthesis in green tea remains open to study. Herein, we report the characterization of a C. sinensis tryptophan-aspartic acid repeat protein (CsWD40) that interacts with myeloblastosis (MYB) and basic helix-loop-helix (bHLH) transcription factors (TFs) to regulate the biosynthesis of flavan-3-ols. Full length CsWD40 cDNA was cloned from leaves and was deduced to encode 342 amino acids. An in vitro yeast two-hybrid assay demonstrated that CsWD40 interacted with two bHLH TFs (CsGL3 and CsTT8) and two MYB TFs (CsAN2 and CsMYB5e). The overexpression of CsWD40 in Arabidopsis thaliana transparent testa glabra 1 (ttg1) restored normal trichome and seed coat development. Ectopic expression of CsWD40 alone in tobacco resulted in a significant increase in the anthocyanins of transgenic petals. CsWD40 was then coexpressed with CsMYB5e in tobacco plants to increase levels of both anthocyanins and PAs. Furthermore, gene expression analysis revealed that CsWD40 expression in tea plants could be induced by several abiotic stresses. Taken together, these data provide solid evidence that CsWD40 partners with bHLH and MYB TFs to form ternary WBM complexes to regulate anthocyanin, PA biosynthesis, and trichome development. [ABSTRACT FROM AUTHOR]