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Sheath blight disease (ShB) has a severe impact on the production of rice. ABI3/VP1-like 1 (RAVL1) negatively regulated the rice defense mechanism against ShB, however, this regulatory mechanism is not clearly understood. In this study, we identified that indeterminate domain 3 (IDD3) was positively regulated by RAVL1. Further, chromatin immunoprecipitation (ChIP) assay, yeast one-hybrid assay and transient expression assay indicated a direct binding between RAVL1 and the IDD3 promoter region. IDD3 was ubiquitously expressed in different tissues and at different stages, and its expression was significantly enhanced by Rhizoctonia solani infection. IDD3 exhibited transcription activation activity in yeast and IDD3-GFP was found to be localized in the nucleus. IDD3 mutants exhibited no significant differences in response to ShB, while IDD3 overexpressors were more susceptible to ShB compared with wild type (WT) plants. Furthermore, IDD3 repressors were less susceptible to R. solani than WT plants. Interestingly, the expression of brassinosteroid-related genes (D2, D11 and BRI1) was lower in IDD3 repressors and higher in IDD3 overexpressors compared with WT. However, the ChIP assay revealed that IDD3 did not directly bind to the D2 and D11 promoters. Overexpression of IDD3 in BRI1 mutant d61-1 inhibited the activity of IDD3, reducing its susceptibility to ShB compared with IDD3 overexpressor and WT plants, indicating that IDD3 negatively regulated the rice defense mechanism against ShB by activating the BR signaling pathway. Thus, our analyses provided information to enhance the understanding of the rice defense mechanism against ShB.

Plasmodiophora brassicae is an obligate intracellular parasitic protist that causes clubroot disease on cruciferous plants. So far, some low-molecular-weight secreted proteins from P. brassicae have been reported to play an important role in plant immunity regulation, but there are few reports on its high-molecular-weight secreted proteins. In this study, 35 putative high-molecular-weight secreted proteins (>300 amino acids) of P. brassicae (PbHMWSP) genes that are highly expressed during the infection stage were identified using transcriptome analysis and bioinformatics prediction. Then, the secretory activity of 30 putative PbHMWSPs was confirmed using the yeast signal sequence trap system. Furthermore, the genes encoding 24 PbHMWSPs were successfully cloned and their functions in plant immunity were studied. The results showed that ten PbHMWSPs could inhibit flg22-induced reactive oxygen burst, and ten PbHMWSPs significantly inhibited the expression of the SA signaling pathway marker gene PR1a. In addition, nine PbHMWSPs could inhibit the expression of a marker gene of the JA signaling pathway. Therefore, a total of 19 of the 24 tested PbHMWSPs played roles in suppressing the immune response of plants. Of these, it is worth noting that PbHMWSP34 can inhibit the expression of JA, ET, and several SA signaling pathway marker genes. The present study is the first to report the function of the high-molecular-weight secreted proteins of P. brassicae in plant immunity, which will enrich the theory of interaction mechanisms between the pathogens and plants. [ABSTRACT FROM AUTHOR]

Although coping with an abusive boss can be psychologically demanding, those who suffer from leader abuse often stay in these unpleasant relationships, actively managing the way they are viewed in the eyes of their abusive leader (source of the abuse) and coworkers (observers of the abuse). Accordingly, the abusive supervision literature has relied almost exclusively on an emotional appraisal perspective to study the self-image implications following leader abuse. The present study seeks to add to this emerging line of scholarly conversations by presenting a novel theoretical alternative. Specifically, we integrate self-presentation and self-concept orientation perspectives to portray individuals' identity-driven self-image maintenance following leader abuse. We argue that only those with a stronger relational self-concept are likely to be motivated to preserve their identity-consistent self-image and present themselves in positive and socially desirable ways toward both their coworkers and leader, following leader abuse. Using survey data collected from working professionals in China across two field studies, we found support for our hypotheses that when employees with a stronger relational self-concept experienced abusive supervision, they were motivated to help their coworkers as a result of their relational reputation maintenance concerns, and to use ingratiation tactics toward their leader due to their image preservation motives. We also offer insights about both the theoretical and practical implications of our research and discuss study limitations and directions for future research. [ABSTRACT FROM AUTHOR]

Considering their drawbacks of environmental pollution, biodegradable cellulose-based materials are becoming one of the most promising alternative candidates for conventional petroleum-based polymers, which are considered the fundamental materials for dynamical units in human–machine interaction systems. Using an up-to-date hydrogen bond replacement strategy, which means using the highly electronegative F− in polyvinylidene fluoride (PVDF) to replace the intramolecular hydrogen bonds in cellulose for weakening the self-assembly behavior, herein, multilayer-structured piezoelectric nanogenerators (PENGs) composed of cellulose, a small amount of PVDF, and Ba0.7Ca0.3Zr0.2Ti0.8O3 (BCZT) fillers were fabricated via modified tape-casting technology. Due to the hydrogen bond network, which was confirmed using multiple characterization methods, the fillers dispersed uniformly in the matrix. Through changing the inner layer thickness, the output performance of the PENGs can be subtly modulated, which is revealed to be caused by the synergistic effect between the trapped electrons and the inter-squeezing between adjacent particles by employing the band theory. When applied to a pedometer, one of the essential devices for monitoring human health, such a modulation can significantly improve its sensitivity. The water contact angle test also indicates their potential for use in humid environments. Compared with some typical cellulose-based PENGs, our device shows outstanding performance in PD/F, defined as the power density triggered by unit force, indicating our PENG's low energy consumption characteristic. [ABSTRACT FROM AUTHOR]

Polymer-based dielectric capacitors, which have two main branches of PVDF-based and PI-based systems, show the advantages of ease of processing and good energy storage capacity over bulk and epitaxy thin films. Nevertheless, both suffer from the drawbacks of being derived from petroleum-based materials and polluting the environment during the post-treatment process. As the most abundant natural polymer on earth, cellulose, which has eco-friendly characteristics and good voltage endurance, has been studied as the best candidate. Owing to the strong self-assembly behavior of the hydrogen bond network in cellulose chains, it is challenging to prepare multilayered cellulose-based films, which have caused a bottleneck in their development. Herein, using the strategy of hydrogen bond replacement, such behavior was weakened, and the sandwich-structured cellulose-based dielectric capacitors of BaTiO3@cellulose/PVDF were successfully fabricated through a modified tape-casting technology. The structure characterization revealed that the cellulose, PVDF, and BaTiO3 particles were bonded through hydrogen bonds, and an energy storage density as high as 27.2 J cm−3 was obtained. Both the band theory and finite element simulation were employed to reveal the excellent electric breakdown strength, which is thought to be the main reason for such a good performance. The reliability measurements showed great potential in practical application, and good power density was also achieved. Besides, the sandwich-structured BaTiO3@cellulose/PVDF has better mechanical properties than the single-layered one. Our study provides a solid theoretical basis for fabricating high-performance biomass-based dielectric capacitors. [ABSTRACT FROM AUTHOR]