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16 results on '"Wang, Xi-le"'

16. [Immunogenicity of envelope glycoprotein gene of reticuloendotheliosis virus expressed in insect cell].

Author

Wang XL, Zhang Z, Jiang SJ, and Cui ZZ

Subjects
Animals, Antibodies, Viral blood, Blotting, Western, Chickens, Fluorescent Antibody Technique, Indirect, Recombinant Proteins immunology, Reticuloendotheliosis virus genetics, Spodoptera cytology, Spodoptera genetics, Genes, env, Reticuloendotheliosis virus immunology, Vaccines, Synthetic immunology, Viral Envelope Proteins immunology, Viral Vaccines immunology
Abstract

A recombinant baculovirus expressing reticuloendotheliosis virus env gene was constructed with Bac-to-Bac Baculovirus Expression Systems. After transfecting the recombinant virus into Sf9 cells for 3 days, REV env can be detected by indirect immunofluorescence antibody assay (IFA) and Western blot with specific monoclonal antibodies of REV. The oil-water emulsion vaccine was then produced using this infected Sf9 cells lycates and inoculated SPF chickens to validate the immunogenicity for REV. The results show that special anti-REV antibody can maintain more than 45 days and resist the infection of REV viruses. This is the first success to induce anti-REV antibody in chickens by none-live viruses.

Published
2005

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