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A major risk factor for glaucoma, the first leading cause of irreversible blindness worldwide, is the decellularisation of the trabecular meshwork (TM) in the conventional outflow pathway. Stem cell‐based therapy, particularly the utilisation of induced pluripotent stem cells (iPSCs), presents an enticing potential for tissue regeneration and intraocular pressure (IOP) maintenance in glaucoma. We have previously observed that differentiated iPSCs can stimulate endogenous cell proliferation in the TM, a pivotal factor in TM regeneration and aqueous humour outflow restoration. In this study, we investigated the response of TM cells in vivo after interacting with iPSC‐derived cells and identified two subpopulations responsible for this relatively long‐term tissue regeneration: ATP Binding Cassette Subfamily G Member 2 (ABCG2)‐positive cells and Nestin (NES)‐positive cells. We further uncovered that alterations of these responsive cells are linked to ageing and different glaucoma etiologies, suggesting that ABCG2+ subpopulation decellularization could serve as a potential risk factor for TM decellularization in glaucoma. Taken together, our findings illustrated the proliferative subpopulations in the conventional outflow pathway when stimulated with iPSC‐derived cells and defined them as TM precursors, which may be applied to develop novel therapeutic approaches for glaucoma. [ABSTRACT FROM AUTHOR]

Hydraulic servo control systems mostly adopt servo valve and proportional valve as control components, which has the advantages of high power to weight ratio, high accuracy and fast response, and is widely used in industry, agriculture, light textile industry and transportation. However, the structure of servo valve and proportional valve is complex, the cost is high, and the quality of oil are required. Adopting incremental digital valve with the advantages of simple structure, low cost, strong anti-pollution ability and convenient maintenance as the control component of servo control system has a wide application prospect. Taking the incremental digital valve controlled cylinder position control system as research object, based on the structure and the operating principle of the incremental digital valve, the mathematical model of the position control system was established. And based on MATLAB/Simulink, the simulation model of the position control system was established. Then, the dynamic performance of the incremental digital valve controlled cylinder position control system with different input signals and structural parameters were compared and analyzed through simulation. Finally, the incremental digital valve-controlled cylinder position control system experiment platform was built to test the dynamic performance under different working conditions. Through combining simulation with experiment, the feasibility of incremental digital valve principle was verified, the dynamic response ability of the incremental digital valve control cylinder position control system under different conditions was analyzed, and the main factors influencing the dynamic response ability were found, which provides the theoretical and experimental basis for the structure improvement of incremental digital valve and the dynamic performance optimization. [ABSTRACT FROM AUTHOR]

A major risk factor for glaucoma, the first leading cause of irreversible blindness worldwide, is the decellularisation of the trabecular meshwork (TM) in the conventional outflow pathway. Stem cell-based therapy, particularly the utilisation of induced pluripotent stem cells (iPSCs), presents an enticing potential for tissue regeneration and intraocular pressure (IOP) maintenance in glaucoma. We have previously observed that differentiated iPSCs can stimulate endogenous cell proliferation in the TM, a pivotal factor in TM regeneration and aqueous humour outflow restoration. In this study, we investigated the response of TM cells in vivo after interacting with iPSC-derived cells and identified two subpopulations responsible for this relatively long-term tissue regeneration: ATP Binding Cassette Subfamily G Member 2 (ABCG2)-positive cells and Nestin (NES)-positive cells. We further uncovered that alterations of these responsive cells are linked to ageing and different glaucoma etiologies, suggesting that ABCG2 + subpopulation decellularization could serve as a potential risk factor for TM decellularization in glaucoma. Taken together, our findings illustrated the proliferative subpopulations in the conventional outflow pathway when stimulated with iPSC-derived cells and defined them as TM precursors, which may be applied to develop novel therapeutic approaches for glaucoma., (© 2024 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.)