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Sea cucumber-derived fungi have attracted much attention due to their capacity to produce an incredible variety of secondary metabolites. Genome-wide information on Aspergillus micronesiensis H39 obtained using third-generation sequencing technology (PacBio-SMRT) showed that the strain contains nonribosomal peptide synthetase (NRPS)-like gene clusters, which aroused our interest in mining its secondary metabolites. 11 known compounds (1–11), including two γ-aromatic butenolides (γ-AB) and five cytochalasans, were isolated from A. micronesiensis H39. The structures of the compounds were determined by NMR and ESIMS, and comparison with those reported in the literature. From the perspective of biogenetic origins, the γ-butyrolactone core of compounds 1 and 2 was assembled by NRPS-like enzyme. All of the obtained compounds showed no inhibitory activity against drug-resistant bacteria and fungi, as well as compounds 1 and 2 had no anti-angiogenic activity against zebrafish. [ABSTRACT FROM AUTHOR]

If municipal wastewater can be treated by the completely autotrophic nitrogen removal over nitrite (CANON) process, it will greatly reduce the energy consumption of municipal wastewater treatment. The CANON reactor with a fiber carrier was started up by seeding nitrosation sludge and anaerobic ammonia oxidation (ANAMMOX) sludge in the continuously stirred tank reactor (CSTR). An ABR decarbonization system was added to the front of the CANON system to build the ABR decarbonization-CANON coupling process to examine carbon and nitrogen removal characteristics of the whole system. The high throughput sequencing technology of MiSeq was also employed to analyze the structure of the microbial community before and after the reactivation. The results showed that mixing nitrosation sludge and ANAMMOX sludge in the CSTR reactor under controlled parameters (DO of 0.5-2 mg·L -1 ; HRT for 6 h; pH of 8) allowed the CANON system to successfully start within 55 d, with a TN removal rate of 81%-87% and ammonia nitrogen load of 0.195 kg·(m 3 ·d) -1 . The effluent COD concentration of the ABR decarbonizing system did not adversely affect the subsequent CANON system, and the TN removal rate of the ABR decarbonization-CANON process was between 74% and 87%. Additionally, the average concentration of COD in the effluent was 40 mg·L -1 . At the same time, the Proteobacteria gate significantly improved after the CANON system began, and the proportion of Sphingobacteria decreased to 6.8%. Nitrifying bacteria and anaerobic ammonia oxidizing bacteria in the CANON system continuously eliminated the inferior bacterial groups to become the dominant group in the reactor. The integrated ABR decarbonization-CANON process had a positive effect on the denitrification and decarbonization of urban sewage.