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Contrapose the highly integrated, complex working conditions and many kinds of faults of aircraft electro hydrostatic actuator(EHA), to diagnose the typical fault of EHA effectively, a fault diagnosis algorithm based on convolutional neural networks (CNN) and support vector machine(SVM) was proposed. Firstly, the fault date sets are entered on CNN for adaptive feature extraction, then the output of the fully connected layer of CNN are classified by using SVM. To improve the performance of SVM, dynamic inertia weight adaptive particle swarm optimization (IWAPSO) was used to optimize the SVM parameters. Finally, the sensitivity of SVM to noise was reduced by introducing ramp loss function. The results show that the accuracy of SVM after parameter optimization is 12.6% higher than that of standard SVM and 17.3% higher than CNN. The SVM based on the ramp loss function showed better robustness when using noisy test sets.

WWP2 is a HECT-type E3 ubiquitin ligase that regulates various physiological and pathological activities by binding to different substrates, but its role in atherosclerosis (AS) remains largely unknown. The objective of the present study is to investigate the role and underlying molecular mechanisms of WWP2 in endothelial injury. We found that WWP2 expression is significantly decreased in Apolipoprotein E (ApoE)–/– mice. Overexpression of WWP2 attenuates oxidative stress and inflammation in AS mice, while knockdown of WWP2 has opposite effects. WWP2 overexpression alleviates oxidized low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cell (HUVEC) injury, evidenced by the decreased oxidative stress levels and the secretion of inflammatory cytokines. Programmed cell death 4 (PDCD4) is identified as a potential substrate of WWP2. Co-immunoprecipitation (Co-IP) further demonstrates that WWP2 interacts with PDCD4, which is enhanced by ox-LDL treatment. Furthermore, the level of PDCD4 ubiquitination is significantly increased by WWP2 overexpression under the condition of MG132 treatment, while WWP2 knockdown shows opposite results. Subsequently, rescue experiments demonstrate that WWP2 knockdown further aggravates oxidative stress and inflammation in ox-LDL-treated HUVECs, while knockdown of PDCD4 alleviates this effect. Moreover, the use of sn-protoporphyrin (SnPP), an inhibitor of HO-1 pathway, confirms that PDCD4 enhances endothelial injury induced by ox-LDL through inhibiting HO-1 pathway. In conclusion, our results suggest that WWP2 protects against atherosclerosis progression via the PDCD4/HO-1 pathway, which may provide a novel treatment strategy for atherosclerosis.

Frying is a critical process in the food industry, where selecting appropriate vegetable oils is key to achieving optimal results. In this study, French fries were fried at 175 °C with five different oils, the changes in the physicochemical indexes and free radical scavenging rate of the oils during the frying process were investigated, and the most suitable oils for frying were identified through comparative analysis using principal component analysis (PCA). We assessed the frying performances of hot-pressed high-oleic-acid rapeseed oil (HHRO), cold-pressed high-oleic-acid rapeseed oil (CHRO), soybean oil, rice bran oil, and palm oil utilizing principal component analysis over an 18 h period. The HHRO and CHRO showed lower acid values (0.31, 0.26 mg/g), peroxide values (2.09, 1.96 g/100 g), p-anisidine values (152.48, 178.88 g/mL), and total polar compound percentages (27.60%, 32.10%) than other oils. Furthermore, both the HHRO and CHRO demonstrated enhanced free radical scavenging abilities, indicative of their higher antioxidant capacities, as corroborated by the PCA results. Benzopyridine, 3-monochloropropane-1,2-diol ester, squalene, tocopherols, and polyphenol from the HHRO and CHRO during frying were compared. A comprehensive examination of harmful substances versus nutrient retention during frying revealed that the HHRO contained fewer hazardous compounds, while CHRO retained more nutrients. Therefore, this study analyzes the oxidation regulation of HHRO in frying applications, highlights the prospects of HHRO for frying in terms of health and economy, and contributes valuable insights for informed vegetable oil selection within the food industry. [ABSTRACT FROM AUTHOR]

Competition in China's electric vehicle industry has intensified significantly in recent years. The production mode of power batteries, serving as the pivotal component in these vehicles, has emerged as a critical challenge for electric vehicle manufacturers. We considered a system comprising an electric vehicle (EV) manufacturer with power battery production technology and another EV manufacturer lacking power battery production technology. In the context of carbon trading policy, we constructed and solved Cournot competitive game models and asymmetric Nash negotiation game models in the CC, PC, and WC modes. We examined the decision-making process of electric vehicle manufacturers regarding power battery production modes and carbon emission reduction strategies. Our research indicates the following: (1) The reasonable patent fee for power batteries and the wholesale price of power batteries can not only compensate power battery production technology manufacturers for the losses caused by market competition but can also strengthen the cooperative relationship between manufacturers. (2) EV manufacturers equipped with power battery production technology exhibit higher profitability within the framework of a perfectly competitive power battery production mode. Conversely, manufacturers lacking power cell production technology demonstrate greater profitability when operating under a more collaborative power cell production mode. (3) Refraining from blindly persisting with and advocating for carbon emission reduction measures is advisable for manufacturers amidst rising carbon trading prices. [ABSTRACT FROM AUTHOR]

Aims The main purpose of this study was to study the therapeutical effect of oroxylin A glucuronide (OAG) on methicillin-resistant Staphylococcus aureus (MRSA). Methods and results By substrate peptide reaction-based fluorescence resonance energy transfer (FRET) screening, we identified that OAG was an efficient inhibitor of Sortase A (SrtA) with an IC50 of 45.61 μg mL−1, and achieved efficacy in the treatment of Staphylococcus aureus (S. aureus) infections. We further demonstrated that OAG inhibited the adhesion of the S. aureus to fibrinogen, the surface protein A anchoring and diminished biofilm formation. Results obtained from fluorescence quenching assay elucidated a direct interaction between OAG and SrtA. Employing molecular dynamics simulations, we proved that OAG binds to the binding sites of R197, G192, E105, and V168 in the SrtA. Notably, OAG exhibited a robust therapeutic effect in a MRSA-induced pneumonia model. Conclusions We identified that OAG as a novel class of reversible inhibitors of SrtA, combats MRSA-induced Infections. [ABSTRACT FROM AUTHOR]