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旨在为生物处理黄曲霉毒素污染的粮食饲料提供技术支持，以活性炭（SH）为原料进行改性制备磁性活性炭（CSH），选用能高效降解黄曲霉毒素B1（AFB1）的芽孢杆菌属菌株WTX1与CSH载体共培养构建磁性菌载体（FCSH），脱除受污染玉米中的AFB1，对CSH进行BET比表面积、氮吸附-脱附等温线及其对AFB1的等温吸附曲线分析，考察FCSH构建条件和FCSH吸附条件对AFB1脱除效果的影响，并利用傅里叶变换红外光谱（FTIR）和扫描电镜对吸附AFB1前后的FCSH进行表征。结果表明：CSH为大孔结构，其对AFB1的等温吸附符合Freundlich方程；在固定化时间16 h、固定化转速和吸附转速160 r/min、固定化温度和吸附温度35 ℃、吸附pH 8、振荡时间48 h条件下，FCSH对AFB1脱除率为97.45%；通过FTIR及扫描电镜证实了FCSH对AFB1的吸附作用，且FCSH对AFB1的吸附效果强于CSH。综上，所制备的FCSH能高效脱除玉米中的AFB1。Aiming to provide technical support for biological treatment of aflatoxin-contaminated grain feed, magnetic activated carbon (CSH) was prepared through modification from activated carbon(SH), and the strain of Bacillus spp. WTX1, which was capable of efficiently degrading aflatoxin B1 (AFB1), was selected to co-culture with the CSH carrier to construct the magnetic bacterial carrier (FCSH) for the removal of AFB1 from contaminated maize. BET specific surface area, nitroqen adsorption-desorption isotherms and isotherm adsorption curves for AFB1 of CSH were analyzed, and the effect of FCSH construction conditions and FCSH adsorption conditions on the effect of AFB1 removal were investigated. Fourier transform infrared spectrum (FTIR) and scanning electron microscope were used to characterize FCSH before and after adsorption of AFB1. The results showed that CSH had a macroporous structure, and its isothermal adsorption of AFB1 conformed to the Freundlich equation. The removal rate of AFB1 by FCSH could reach 97.45% under the conditions of immobilization time 16 h, immobilization and adsorption speed 160 r/min, immobilization and adsorption temperature 35 ℃, adsorption pH 8, and shaking time 48 h. The adsorption of FCSH on AFB1 was confirmed by FTIR and scanning electron microscope, and the adsorption effect of FCSH on AFB1 was stronger than that of CSH.In conclusion, the prepared FCSH can effectively remove AFB1 from maize.

[Objective] The desert plant Karelinia caspia has a broad-spectrum of stress resistance, including salt/alkali tolerance and high temperature tolerance, which is a precious natural plant germplasm native to Xinjiang. It has been noted that mining the genes involved in extreme temperature and salt tolerance of K . caspia as well as analyzing their expression under stress is critical for using these genes to enhance crop stress resistance, increase yield, and promote the exploration and utilization of the genetic resources of the desert plants. [Methods] Potted K . caspia seedlings were subjected to different durations of high temperature (45 ℃), low temperature (4 ℃), and high salt (400 mmol/L Na+ ) stress. Root, stem, and leaf tissues were collected to analyze the expression of the HTR gene family (KcHTRs) by using RT-PCR and other techniques, and analyze their stress resistance and optimal induction conditions for protein expression by a prokaryotic expression system. [Results] (1) Seven of the KcHTR gene family members were screened and cloned from the transcriptomic data obtained from high temperature treatments of K . caspia. By constructing prokaryotic expression vectors and inducing expression, cDNA lengths of the KCHTR gene family were determined to be between 708 and 789 bp, and the molecular weights of the expressed protein was between 27 and 29 kD. (2) It was found that the optimal expression system of E. coli BL21 was 0.8 at OD600, IPTG concentration 0.5 mmol/L, and induction time 8-10 h at the optimum temperature of 37 ℃. (3) Recombinant E. coli was treated with simulated high temperature, low temperature and salt stress, and the results showed that KcHTRs enhanced the host tolerance to the stresses to certain extent. [Conclusion] The results showed that KcHTRs has broad-spectrum of stress resistance, mainly extreme temperature, and actively responds to stress conditions.

ObjectiveThe study utilized human transcriptome microarray to explore biomarkers for diagnosing drug-induced liver injury （DILI） caused by anti-tuberculosis drugs.MethodsA 6-month follow-up study was conducted on 152 patients treated with anti-tuberculosis drugs in designated hospitals in Shanghai. The blood samples were collected at the 0， 2， 4， 8， 12 and 24 weeks after treatment. According to the clinical biochemical indicators， the research subjects were divided into DILI cases （34 cases） and Control cases （118 cases）. Single factor analysis was conducted on the influencing factors between the two groups. In a 1∶1 matched DILI-control study， RNA samples of 13 pairs of cases were sequenced by the whole transcript expression mRNA array. Differentially expressed genes （DEGs） were screened by Hotelling's T2 value sequencing and the expression trend analysis of genes by STEM （short-time series expression miner）， and the functional enrichment and pathway analysis of DEGs were carried out.ResultsIn total 152 clinical cases， weight of patients was a risk factor for the occurrence of hepatotoxicity caused by anti-tuberculous drugs. Based on the analysis results of mRNA array， 513 DEGs were screened by Hotelling's T2 value sequencing method， which were enriched in 32 annotations of GO （Gene Ontology） analysis and 10 pathways of KEGG （Kyoto encyclopedia of genes and genomes） analysis. One differential expression pattern was screened by STEM， which was enriched in 2 biological process notes of GO. Among them， the key genes AIM2， CD86， CXCL10 and non-coding RNAs SCARNA10， SNHG10 and SNORD105 are potential biomarkers of DILI caused by anti-tuberculosis drugs.ConclusionIn this research for biomarkers conducted on cases with liver injury caused by anti-tuberculosis drugs， biological pathways associated with hepatotoxicity are identified and a series of key genes related with drug-induced liver injury are found， which provides the basis for mechanism study and searching for earlier and more sensitive biomarkers.