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In view of problems of poor ability of automation cutting, bad stability and difficult real-time identification of dynamic load of cutting of extra-heavy type rock roadheader, a kind of dynamic load identification device for cutting mechanism of rock roadheader based on RBF neural network was designed according to characteristics of mining roadway, and designs of hardware and software of the identification device were introduced in details. To take industrial computer as analysis center, the device can real-timely monitor signals of cutting motor current and cutting head vibration, and uses multi-sensor fusion technology and RBF neural network to realize signal processing and intelligent analysis, so as to realize feature extraction and recognition of dynamic load of cutting rock and achieve effective distinction of rocks with different hardness.

In 2020, field trials were conducted in Datonghu District in Hunan province according to the split zone design, using rice varieties Taiyou390 and Hyou518 as materials, with conventional machine transplanting (T1), manual seedling throwing (T2) and orderly mechanical seedling-broadcasting (T3), to determine the seedling quality, yield and yield composition, stem dynamics and growth period under different planting methods, and to explore the influence of different planting methods on the growth and development characteristics, grain yield and yield components of the late season rice under the influence of autumn low temperature(ALT). Find out planting methods that have more stable yield and disaster reduction effects under the influence of ALT, and explore their disaster reduction mechanisms to provide a theoretical basis for rice production and disaster prevention and reduction strategies. The results showed that: (1) the seedling quality of the orderly mechanical seedling-broadcasting planting model cultivated by the pot seedling raising technology was significantly better than that of the blanket seedling raising technology. (2) The orderly mechanical seedling-broadcasting model obsessed the highest growth recovery and tillering speed than other models. (3) The orderly mechanical seedling-broadcasting shortened the growth duration of rice, which was reduced by 4-12d and 3-8d, respectively for Taiyou390 and Hyou518 as compared with other models. (4) Under ALT, the panicle-tiller rate, grain filling percentage, harvest index, panicle number, grain weight and grain yield of rice was improved under orderly mechanical seedling-broadcasting model. When compared with T1 and T2, the grain yield under orderly mechanical seedling-broadcasting was increased by 21.7% and 273.5% for Taiyou390, and was increased by 12.9% and 19.6% for Hyou518, respectively. In summary, the orderly mechanical seedling-broadcasting pronouncedly enhanced the performance of late season rice regarding seedling quality, growth recovery and early tillering speed, panicle numbers, grain filling percentage, grain weight and grain yield especially under ALT. The present study demonstrated that the orderly mechanical seedling-broadcasting is a safety rice mechanical production method to guarantee the safe production of late season rice under severely chilling stress. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the safety, efficacy and patient acceptability of burn therapy with Chinese Medicinals in treating pain induced by lumbar intervertebral disc herniation (LDH) in community. Methods: All 684 cases of LDH were provided with Chinese Medicinal burn therapy treatment in this research. The visual analogue scoring (VAS) was applied to evaluate the lumbago and the quality of life of patients before and after treatment. Moreover, logistic regression analysis was applied to analyze the influence factors of curative effect. Results: The total effective rate of analgesia was 77.2%. The total effective rate of patients with severe pain and disease course ≤6 months before the treatment was 91.8 and 90.9%, which was significantly higher than that of patients with mild and moderate pain group (70.7%) with the course of disease >6 months (76.1%). The more severe the lower back pain, the better the curative effect (OR = 4.877), and the shorter the disease course of lumbago, the better (OR = 0.256) the curative effect. The overall effective rate of improvement was 79.7, 80.2 and 80.3%, respectively on the effects of daily life, daily work and walking ability. The incidence of adverse reactions was 3.5%. The patients' satisfaction and approval degree of technical treatment were 84.0 and 86.3%. Conclusions: The treatment with Chinese Medicinal burn therapy to relieve pain induced by LDH was safe and effective. Moreover, patient's compliance was better; the degree of acceptance was high. In addition, the technique is easy to master by doctors in a basic hospital setting, and is suitable for promotion in primary health care institutions. [ABSTRACT FROM AUTHOR]

Montmorillonite is characterized by small particle size, large specific surface area and strong water sensitivity, which results in blocking the pore and throat of the reservoir and in turn affecting its quality. Dissolution characteristics of montmorillonite under alkaline conditions are common concerns in the development of oil fields. Mass contrast, X-ray Diffraction, solution concentration analysis and value analysis of pH methods are commonly used to characterize dissolution of montmorillonite. However, the quality comparison method is complex. Calculation of diffraction pattern by the X-ray Diffraction involves large manual operation errors. The ion concentration analysis method shows a large detection error for infusible elements. Value analysis of the pH method is influenced by temperature changes. The specific surface area, pore volume, pore diameter change, adsorption-desorption curve measured by nitrogen adsorption method was used to characterize the dissolution of montmorillonite under alkaline conditions and is reported in this paper. The experimental conditions were optimized, including the concentration of NaOH solution and filling time (reaction time). The best concentration of NaOH solution was 0.05 mol/L and the best reaction time was 3 h. Results show that the dissolved results of montmorillonite by the nitrogen adsorption method characterized by specific surface area, pore volume, pore diameter and adsorption desorption curve are consistent with the results by the change of the pH value and the concentration of the solution (such as Si4+). Therefore, the method is feasible and can be used to characterize the dissolution of minerals with multiple pores and large specific surface area.

Background: Traditional radiography angles do not adequately reveal the shape and position of the right ventricular pacing electrode., Objective: This study aimed to explore better radiography angles with the help of cardiac computed tomography (CT)., Methods: We analyzed the cardiac CT images of consecutive outpatients from 2018 to 2019. The right anterior oblique (RAO) 30° and the left anterior oblique (LAO) 40° were found to sufficiently display the shape and position of the right ventricular pacing electrode., Results: A total of 214 consecutive outpatients were enrolled, whose average age was 55.0 ± 13.0 years, and 151 were male (70.6%). Through analyzing the cardiac CT images, the α angle (33.7° ± 6.1) and the γ angle (38.8° ± 8.0) were determined. Furthermore, we verified these angles in 48 patients after pacemaker implantation. The results showed that the ratio of the length of right ventricular electrode using the RAO α angle (≈30°) to the posterior-anterior position (PA position) was 1.099 ± 0.157 vs. 1.053 ± 0.182 (the ratio using the traditional RAO 45°) (P < 0.001). We observed that the relationship between the right ventricular active electrode and the ventricular septum was better identified using the LAO γ angle (≈40°) than the traditional 60° angle., Conclusion: With the help of cardiac CT, we found that RAO 30° could better show the shape and length of the right ventricular pacing electrode, and LAO 40° could better show the positional relationship between the pacing electrode and the ventricular septum., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Because of the changed metabolic behaviors of cancer cells, tumor cells uptake a corresponding larger amount of glucose in physiological condition when compared with normal cells. And they were prone to metabolize glucose for generating energy in anaerobic glycolysis ways in order to grow quickly. Anaerobic glycolysis consumes more glucose than aerobic way when the same amount of energy is obtained, which also results in large demand of glucose in tumor cells. This review briefly describes therapy methods related to characteristic mentioned above, and summarizes the research progress of drugs, diagnostic reagents and carriers conjugated with glucose, glucose derivatives or other kinds of sugars for cancer targeting. Furthermore, typically relative research reports from 2012 till now were listed and analyzed.

Genotoxicity of aldicarb and methomyl was explored. The aldicarb and methomyl were diluted by the deionized water respectively, and then five concentrations of aldicarb were generated as 0.002, 0.02, 0.2, 2, 20 microg/L, methomyl as 0.02, 0.2, 2, 20, 200 microg/L. The micronucleus of carp erythrocyte was counted by micronucleus test. The mutation of bacteria was assessed by Ames test. The DNA damage of human lymphocytes was tested by comet assay. The genotoxicity of aldicarb and methomyl was estimated by the three toxicology tests mentioned above. The results showed that, in the micronucleus test, both any concentration of two pesticides were not able to induce higher frequency of micronucleus in carp erythrocyte (p > 0.05). Under condition of metabolic inactivation, although the number of colony with back mutation in any concentration of two pesticides did not exceed the double number of those with spontaneous mutation, the revertants of TA97 strains in the aldicarb 2-20 microg/L and the methomyl 20-200 microg/L were (129.17 +/- 17.00), (129.50 +/- 18.28), (109.83 +/- 10.80) and (114.17 +/- 9.37) entries/plate, respectively, they were significantly greater than those in spontaneous mutation (p < 0.05, p < 0.01). In the methomyl 200 microg/L group, the revertants of TA100 and TA102 strains were (147.83 +/- 23.29) and (275.83 +/- 20.63) entries/plate, respectively, they are significantly higher than that of the control group under condition of metabolic activation (p < 0.05). In comet assay, both the high concentration groups of aldicarb and methomyl resulted in different degrees of DNA damage of human peripheral blood lymphocytes. Compared with deionized water group, all of three indexes of comet assay in the aldicarb 20 microg/L groups and the methomyl 200 microg/L groups were significantly higher (p < 0.01). Despite that both aldicarb and methomyl did not results in damaging chromosome carp erythrocyte and producing apparent mutagenicity, the effect of mutagenicity and DNA damage in human lymphocytes were observed in high concentration groups of both aldicarb and methomyl. Water polluted by aldicarb and methomyl may have the potential adverse effects on the environment and human health.

Objective: To explore the role of bone marrow (BM) imprint in the diagnosis of hematological diseases., Methods: Between January 2002 and June 2008, a total of 3024 cases with BM smears, imprints and sections conducted simultaneously were recruited. There were 1667 males and 1357 females with a median age of 55 years old (range: 7 to 92). The cellularity on imprint and smear was evaluated with the standard cellularity on BM section. With the integrative diagnosis (including all examinations and clinical outcomes) as the standard, the diagnostic accuracy of hematological diseases were compared between BM imprint, smear and section groups. Another 79 cases of lymphoma and 114 cases of plasma cell myeloma (PCM) were selected for a correlation analysis of tumor cell infiltration patterns., Results: BM imprint contained hematopoietic and non-hematopoietic regions and cells retained integrated structure. The cellularity evaluation by imprint was superior to smear overall. In BM imprint group, the diagnostic accuracy for hypersplenism (n = 130), metastatic carcinoma (n = 67), refractory anemia with excess blasts, myeloproliferative neoplasm (n = 174), and PCM (n = 94) were better than smear group (96.9% vs 80.7%, 91.0% vs 76.1%, 92.6% vs 81.5%, 92.5% vs 76.4%, and 97.8% vs 92.6% respectively, all P < 0.05); And the diagnostic accuracy for megaloblastic anemia (n = 69), acute myeloid leukemia (n = 104), refractory cytopenia with unilineage dysplasia (n = 15), refractory cytopenia with multilineage dysplasia (n = 22), and lymphoplasmacytic lymphoma (n = 12) were higher than biopsy section group (100% vs 84.0%, 91.3% vs 74.0%, 86.7% vs 60.0%, 90. 9% vs 72.7%, and 66.6% vs 50.0% respectively, all P < 0.05); And the diagnostic accuracy for myelodysplastic/myeloproliferative neoplasm (n = 26) was higher than smear group (76.3%, P < 0.05) and biopsy section group (78.2%, P < 0.05). Excellent correlations existed between BM imprint and section of the patients with lymphoma or with PCM (r = 0.90, r = 0.78, both P < 0.05)., Conclusions: BM imprint contains the characteristics of both smear and section. BM imprint is superior to smear for an evaluation of cellularity. And it is also better than section for an analysis of cytological changes.

Objective: To study the expression changes of apoptosis related genes induced by cerulenin in multiple myeloma cell line U266 and explore its molecular mechanism., Methods: The expression changes of 96 apoptosis related genes were analyzed by superArray cDNA in U266 cells treated with cerulenin (20 microg/ml) for 12 h. Semi-quantitative RT-PCR was used to confirm the representative expression changes genes, Rip2, caspase 9 and TRAF2., Results: After treated with cerulenin for 12 h, 44 apoptosis related genes expression in the U266 cells were changed, among which 41 were over 2 fold increase and 3 over 2 fold decrease. The expression of caspase 9 was increased markedly, indicating that mitochondria pathway played a key role in cerulenin inducing apoptosis and TRAF2 expression change suggested that nuclear factor (NF) participates in cerulenin inducing apoptosis., Conclusion: The death acceptor signaling pathway and the death acceptor non-dependence signaling pathway co-regulate cerulenin inducing apoptosis in U266 cells. Mitochondria pathway played the key role and nuclear factor (NF) participates in the apoptosis process.

Objective: To determine whether fatty acid synthase (FAS) is expressed in human multiple myeloma( MM) cells and investigate the proliferation inhibition effect of fatty acid synthase inhibitor cerulenin on multiple myeloma cell line U266 and its mechanism., Methods: FAS mRNA expression in human MM cell line U266, RPMI8226 cell was assayed by RT-PCR. The proliferation inhibition rate of U266 cells was assayed by MTr analysis. Cell apoptosis and cycle distribution were evaluated by flow cytometry (FCM)., Results: FAS mRNA was highly expressed in human multiple myeloma cell lines as compared with healthy donor PBMNCs. After U266 cells were treated with cerulenin (the concentrations from 5 microg/ml to 640 microg/ ml) for 24 h, the cell proliferation was markedly inhibited with a dose related manner, while the inhibition rate of human skin fibroblast cells were all lower than 30%. When U266 cells were treated with 20 pjg/ml cerulenin for 12 h and 24 h, the early apoptosis rate revealed by Annexin V/PI were 56. 9% and 69. 3% respectively, being higher than that of the blank controls (4. 3% and 1.8%, P < 0. 01). Cell cycle analysis showed it was blocked in S phase. Conclusion FAS is highly expressed in human MM. Cerulenin could induce apoptosis and inhibit proliferation of U266 cells. FAS might be a new potential target for multiple myeloma treatment.

To investigate the morphological changes of megakaryocytes with nuclear extrusion and nucleocytoplasmic separation, the morphological characteristics of megakaryocytes in peripheral blood films, bone marrow smears, and bone marrow biopsies from 4 newly diagnosed patients with primary myelofibrosis (PMF), myelodysplastic syndrome (MDS), myeloblastic leukemia with maturation (M(2)) and erythroleukemia (M(6)) were studied by using light microscope. The results showed that many kinds of dysmegakaryocytes were observed in bone marrow smears of 4 cases, while in case A (PMF) and case D (M(6)) micromegakaryocytes were ripped apart; in case B (MDS) and case C (M(2)) megakaryocytes were accompanied by nuclear extrusion or nucleocytoplasmic separation, and their bodies were large or giant, the part of nucleus separated from their body and little cytoplasm remained as micromegakaryocytes. The nucleocytoplasmic separation could be displayed by immunocytochemistry stain. It is concluded that the phenomenon of nuclear extrusion and nucleocytoplasmic separation in megakaryocytes suggested the process that dispersed multinuclear releasing towards surround or even totally left the cell body during the megakaryocyte maturation. It also showed that the micromegakaryocytes may be the result of nucleocytoplasmic separation or splittings from multi-separated nucleus.

Making use of the high expression efficiency of maltose-binding protein (MBP) in E. coli, the soluble and the inclusion body forms of MBP were purified to homogeneity by affinity chromatography and by denaturation, refolding and gel filtration respectively. After immunized Balb/C mice with two forms of MBP respectively, 5 clones for each antigen form were found to secrete monoclonal antibodies. The binding experiments showed that two antibodies obtained by the immunization with the refolded form of MBP had high affinity to the denatured MBP.