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Coffee pectin hydrolysate as an additional carbon source, the denitrification performance was explored by analyzing the denitrification effect in denitrifying biofilter(DNBF) reactor under different HRT and m(COD)/m(TN) (C/N) and microbial community characteristics, and compared with the traditional carbon source of sodium acetate. The results showed that the optimal HRT for the coffee pectin hydrolysate system was 1 h, and the optimal C/N was 6. Under these conditions, when the influent TN and NH4+-N were (33.97±2.06) mg/L and (3.09±0.19) mg/L respectively, coffee pectin hydrolysate as carbon source could reduce the effluent TN and NH4+-N to (3.12±0.59) mg/L and(2.19±0.14) mg/L, respectively. The effluent NO3--N was stabilized below 1 mg/L, and the removal rate reached 97.13%. NO2--N had no obvious accumulation. The NO3--N removed by denitrification per unit COD was 0.22 g/g, which was close to that of the control group sodium acetate. The microbial diversity in the system of coffee pectin hydrolysate was better than that of sodium acetate. There were a large quantities of microorganisms with organic degradation function and denitrification function, such as Zoogloea, Ignavibacterium, unclassified_prolixibacitraceae and Longilinea, which ensured the denitrification effect of coffee pectin hydrolysate as a carbon source.

ObjectiveTo provide suggestions for the improvement of the standards of China， the management situation of international organizations and other countries on temperature requirements of food cold chain process was studied.MethodsThe standards between China and Codex Alimentarius Commission （CAC）， International Organization for Standardization （ISO）， the European Union， the United States， Canada and Australia/New Zealand were compared and analyzed. And the issues of most concerning were raised. The suggestion of cold chain logistics temperature control of China was put forward.ResultsThe standards of other countries were basically recommended operating requirements. There were fewer requirements for specific temperatures. However， the standards system in our country was a combination of compulsory and recommended and there were more requirements for specific temperatures. In both domestic and foreign standards， temperature fluctuation was allowed， and the importance of vehicle precooling， monitoring， transportation tools， and so on were emphasized. However， foreign standards were more emphasized on process management. In the future， China should strengthen the establishment of recommendatory operation rules.ConclusionThe standard management on temperature requirement of the food cold chain in China conforms to international standards. The food safety standards are relatively reasonable. China should take good measures to the quality standards， and promote healthy and harmonious development of this industry.

Following the derivation of multiple reducts,an ensemble based classification framework can be constructed,which has been demonstrated to be useful in improving the performance of subsequent learning tasks.The approach called super-reduct is exactly suggested with such thinking.Generally,multiple super-reducts are obtained by randomly adding more extra attributes into the fundamental reduct.Therefore,how to search fundamental reduct is the key to performing super-reduct.In view of this,considering both efficiency and effectiveness,not only attribute group but also ensemble selector is introduced into the mechanism of super-reduct:the device of attribute group is used to speed up the process of searching fundamental reduct,the device of ensemble selector is used to find more robust attributes in the procedure of searching reduct.Comprehensive experiments on 20 UCI data sets show that compared with 4 popular strategies,our approach can not only significantly reduce the computational cost but also provide superior stabilities and accuracies for classification tasks.

Background and purpose: Oral squamous cell carcinoma (OSCC) is the most common subtype of head and neck squamous cell carcinoma (HNSCC), and its pathogenesis is unclear. Nucleolar protein 8 (NOL8), as one of the RNA-binding protein (RBP), plays a key role in the occurrence and development of many kinds of tumors, however its role in OSCC is not clear. This study aimed to investigate the expression level of NOL8 in OSCC and its effects on the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of OSCC. Methods: The expression of NOL8 in HNSCC was analyzed online by gene expression profiling interactive analysis 2 (GEPIA2), tumor immune estimation resource (TIMER), the University of Alabama at Birmingham cancer data analysis portal (UALCAN) and the encyclopedia of RNA interactomes (ENCORI). The mRNA expression level of NOL8 in OSCC cells was detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). siRNA interference technique was used to knock down the expression of NOL8 in CAL-27 cells to form NOL8 knockdown group (si-NOL8-1, si-NOL8-2) and negative control group. CAL-27 and HN6 cells were overexpressed with NOL8 by lentivirus transfection technique to form NOL8 overexpression group and negative control group. Cell counting kit-8 (CCK-8) assay, scratch healing assay and transwell assay were used to detect the effect of NOL8 expression on the proliferation, migration and invasion of OSCC cells. Western blot assay was used to detect the effect of NOL8 on the expression of EMT-related genes including E-cadherin, vimentin and N-cadherin. The effect of NOL8 on the proliferation of OSCC cells in vivo was examed by xenograft formation assays. Results: The online analysis of GEPIA2, TIMER, UALCAN and ENCORI showed that the expression of NOL8 was higher in HNSCC than in normal tissues, and the expression of NOL8 in OSCC was significantly higher than in normal control cells. The relative expression of NOL8 in CAL-27 cells transfected with si-NOL8-1 and si-NOL8-2 was significantly lower compared with the negative control group. The results of CCK-8 assay, scratch healing assay and transwell assay showed that the proliferative ability, cell migration rate and invasion number of CAL-27 cells were significantly decreased after knockdown of NOL8 expression. The relative expression of NOL8 in CAL-27 and HN6 cells transfected with NOL8 was significantly higher compared with the control group. The proliferative ability, cell migration rate and invasion number of CAL-27 and HN6 cells in the overexpression NOL8 group were significantly higher compared with the negative control group. The results of Western blot showed that in CAL-27 cells, the expression of E-cadherin increased and the expressions of N-cadherin and vimentin decreased after NOL8 knockdown, while in CAL-27 and HN6 cells, the expression of E-cadherin decreased and the expressions of N-cadherin and vimentin increased after NOL8 overexpression. The xenograft formation assays showed that the weight of tumor was significantly higher in NOL8 overexpression group than in NOL8 control group. Conclusion: NOL8 is highly expressed in OSCC and can promote the proliferation, migration and invasion of OSCC, which may be related to the process of EMT.

China possesses abundant pumice resources and thereby makes the utilization of pumice in the preparation of pumice aggregate concrete (PAC) a significant strategy for environmental protection and resource conservation. To obtain the effect of pumice pore structure variation on the compressive strength of PAC, PACs with strength classes LC20, LC30, and LC40 were prepared. Moreover, the pore structure of PAC was characterized using nuclear magnetic resonance to investigate the effect of pore structure variation on the compressive strength of PAC. Results showed that the higher the coarse aggregate content of PAC, the higher the percentage of large capillary and non-capillary pore sizes of PAC, corresponding to higher porosity and lower compressive strength. The hydration products in PAC continuously fill in the pore structure, the proportion of large capillary pores and non-capillary pore size gradually decreases, the proportion of small capillary pores and medium capillary pore size gradually increases, the pumice concrete matrix gradually becomes dense, and the compressive strength increases. The prediction model of the pore structure and compressive strength is established based on gray theory, and the relative error between predicted and tested values is not significant, which can effectively predict its compressive strength. It provides effective guidance for the engineering practical application of PAC.

This paper summarized the nursing experience in a case of chronic renal insufficiency treated with combination of Traditional Chinese Medicine (TCM) enema and Baduanjin exercise, mainly including nursing assessment, routine nursing, procedures of TCM enema and Baduanjin exercise, and matters need attention in the treatment. The combination treatment of TCM enema and Baduanjin exercise was effective to relieve the symptoms of constipation and fatigue. (本文总结中药灌肠联合健身八段锦治疗1例慢性肾功能不全患者的护理经验, 主要包括护理评估、常规护理、中药灌肠及八段锦操作方法和注意事项等, 通过开展中西医结合干预措施, 能够有效改善了患者便秘、乏力等症状。)

During the dehydration process, tissue cells in different locations undergo diffusion and evaporation of water, and other microstructural changes such as cell shrinkage, rupture, and pore formation often occur. Changes in the cell microstructure affect the moisture and temperature distributions in the material and the quality of the material. Therefore, studying the change law of the cell microstructure at different positions is vital for improving material quality. This study used microscopic observation and image processing technology to study the changes in the morphology of cells in different positions of the sample during the process of water migration, and the changes in size parameters (relative diameter, relative perimeter, relative area, and area) and shape parameters (roundness and elongation) of cells at different positions at 0 oC and 8 oC were analyzed. The results showed that during the dehydration process, the maximum value of the size parameter at different moments at 0 oC was obtained at the middle of the section center and the lower tissue edge. The maximum value at 8 oC was obtained at the center of the section. The relative cell diameter, relative perimeter, and relative area changes exhibited the same trend, but the relative area changed more. The shape parameters were distributed within a range without obvious uniformity. At 0 oC, the cell roundness was roughly distributed in the range of 0.85 to 0.95, and the cell elongation rate was concentrated in the range of 1.2 to 1.8. At 8 oC, the cell roundness was roughly distributed in the range of 0.80 to 0.90, and the cell elongation rate was concentrated between 1.2 and 1.9. At 0~30 min, the change in the dimensional parameters at each position increased with an increase in temperature. Compared to that at 0 oC, the shrinkage of the cells was significantly higher at 8 oC during the dehydration process, and the overall change trend of the cell shape was more uniform.

Automobile exhaust always makes up a significant portion of the causes of air pollution, and new energy vehicles (NEV) are being investigated as a potential solution to the problem of urban air pollution. Through a number of policy incentives and infrastructure improvements, the Chinese government encourages the quick development of the new energy automotive sector. National air quality has also improved dramatically in recent years. In this context, the primary goal of this study is to explore whether there is a significant positive correlation between the popularization of NEVs and air quality. This study looks for data on the penetration rate of NEVs in various provinces and cities across the country, as well as the data on the number of days with air quality, and makes a visual chart to show the change trend from 2013 to 2022. Finally, policy recommendations are given. The government should adapt to local conditions and continue to boost the infrastructure of NEVs. In addition, clean energy power generation, sustainable production and recycling should be actively promoted from the perspective of the whole life cycle of NEVs.

A set in the complex plane which involves n parameters in [0, 1] is given to localize all eigenvalues different from 1 for stochastic matrices. As an application of this set, an upper bound for the moduli of the subdominant eigenvalues of a stochastic matrix is obtained. Lastly, we fix n parameters in [0, 1] to give a new set including all eigenvalues different from 1, which is tighter than those provided by Shen et al. (Linear Algebra Appl. 447 (2014) 74-87) and Li et al. (Linear and Multilinear Algebra 63(11) (2015) 2159-2170) for estimating the moduli of subdominant eigenvalues.

[Purpose/significance] Digital transformation has an increasingly deep and strong impact on all aspects of archives management, exploring innovative management of enterprise archives in such context would provide a reference to open up a new path of archive management in line with the digital age. In the context of innovation-driven development and digital transformation, exploring innovative management of enterprise archives can provide a reference for enterprises to open up a new path of archive management in line with the digital age and to achieve reform and innovation of their archives work. [Method/process] According to the characteristics of enterprise archives innovative management under digital transformation, this paper regarded the knowledge-based archive management innovation system as a basis, interpreting the 4 innovation dimensions comprised in this theoretical construct, and further summarizing the related studies to clarify the core points of each innovation dimension. In view of the constraints of institutional innovation and the necessity of process innovation, this paper added the dimension of “process innovation” on the basis of retaining the three original dimensions, including “concept innovation”, “management innovation” and “technology innovation”. Relying on this framework, it explored the concrete practical path of enterprise archives innovation management activities under the background of digital transformation. [Result/conclusion]It is found that innovative management of enterprise archives can be achieved by four ways in the context of digital transformation, namely moving from traditional management to green management, from absolute control to employee empowerment, from traditional technology to emerging technologies, and from serial processes to integrated processes.

Mass spectrometry imaging (MSI) is a high-throughput imaging technique capable of the qualitative and quantitative in situ detection of thousands of ions in biological samples. Ion image representation is a technique that produces a low-dimensional vector embedded with significant spectral and spatial information on an ion image, which further facilitates the distance-based similarity measurement for the identification of colocalized ions. However, given the low signal-to-noise ratios inherent in MSI data coupled with the scarcity of annotated data sets, achieving an effective ion image representation for each ion image remains a challenge. In this study, we propose DeepION, a novel deep learning-based method designed specifically for ion image representation, which is applied to the identification of colocalized ions and isotope ions. In DeepION, contrastive learning is introduced to ensure that the model can generate the ion image representation in a self-supervised manner without manual annotation. Since data augmentation is a crucial step in contrastive learning, a unique data augmentation strategy is designed by considering the characteristics of MSI data, such as the Poisson distribution of ion abundance and a random pattern of missing values, to generate plentiful ion image pairs for DeepION model training. Experimental results of rat brain tissue MSI show that DeepION outperforms other methods for both colocalized ion and isotope ion identification, demonstrating the effectiveness of ion image representation. The proposed model could serve as a crucial tool in the biomarker discovery and drug development of the MSI technique.