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Discusses strategies used to analyze the content of human Chromosome 7 for the International Human Genome Project. Significance of identifying the parts of the genome; Description of the clone-based hierarchical shotgun strategy; Use of the fluorescence-based DNA sequencing instruments in the analysis.

The recently initiated human genome project is a large international effort to elucidate the genetic architecture of the genomes of man and several model organisms. The initial phases of this endeavor involve the establishment of rough blueprints (maps) of the genetic landscape of these genomes, with the long-term goal of determining their precise nucleotide sequences and identifying the genes. The knowledge gained by these studies will provide a vital tool for the study of many biologic processes and will have a profound impact on clinical medicine. [ABSTRACT FROM AUTHOR]

A soluble antigen has been obtained from sheep erythrocytes by sonication. This material is not sedimented by centrifugation by 3.5 × 106 g minutes. but is excluded from Sephadex G-200 gel. The soluble preparation elicits an antibody response in vivo and reacts with antibody prepared against intact sheep erythrocytes. The soluble antigen is as effective as erythrocytes in inducing delayed-type hypersensitivity and in eliciting the delayed-type reaction. [ABSTRACT FROM AUTHOR]