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1. Characterization of an Escherichia coli O157:H7 O-antigen deletion mutant and effect of the deletion on bacterial persistence in the mouse intestine and colonization at the bovine terminal rectal mucosa

Author

Haiqing Sheng, Ji Youn Lim, Watkins, Maryann K., Minnich, Scott A., and Hovde, Carolyn J.

Subjects
Escherichia coli -- Genetic aspects, Mutation (Biology) -- Analysis, Polysaccharides -- Structure, Intestines -- Microbiology, Intestines -- Research, Biological sciences
Abstract

Escherichia coli O157:H7 mutant defective in the synthesis of the lipopolysaccharide side chain (O antigen) by deletion of a putative perosamine synthetase gene (per) in the rfb cluster was generated to study the role of the O antigen in persistence and colonization in the animal host. The data results suggested that the O polysaccharide side chain of Escherichia coli O157:H7 lipopolysaccharide (LPS) is a required factor for efficient bovine intestinal colonization.

Published
2008

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