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5. Methyl formate as a hydrogen energy carrier

Author

Sang, Rui, Wei, Zhihong, Hu, Yuya, Alberico, Elisabetta, Wei, Duo, Tian, Xinxin, Ryabchuk, Pavel, Spannenberg, Anke, Razzaq, Rauf, Jackstell, Ralf, Massa, Jonas, Sponholz, Peter, Jiao, Haijun, Junge, Henrik, and Beller, Matthias

Published
2023
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8. Complete genome sequence of Nguyenibacter sp. L1, a phosphate solubilizing bacterium isolated from Lespedeza bicolor rhizosphere

Author

Xiao Li Li, Xin Yang Lv, Jun Bin Ji, Wei Duo Wang, Ji Wang, Cong Wang, Hai Bin He, Ai Ling Ben, and Ting Li Liu

Subjects
Nguyenibacter sp. L1, whole genome, gene functions, nitrogen fixation, phosphate dissolution, Microbiology, QR1-502
Abstract

Phosphorus (P) deficiency is a predominant constraint on plant growth in acidified soils, largely due to the sequestration of P by toxic aluminum (Al) compounds. Indigenous phosphorus-solubilizing bacteria (PSBs) capable of mobilizing Al-P in these soils hold significant promise. A novel Al-P-solubilizing strain, Al-P Nguyenibacter sp. L1, was isolated from the rhizosphere soil of healthy Lespedeza bicolor plants indigenous to acidic terrains. However, our understanding of the genomic landscape of bacterial species within the genus Nguyenibacter remains in its infancy. To further explore its biotechnological potentialities, we sequenced the complete genome of this strain, employing an amalgamation of Oxford Nanopore ONT and Illumina sequencing platforms. The resultant genomic sequence of Nguyenibacter sp. L1 manifests as a singular, circular chromosome encompassing 4,294,433 nucleotides and displaying a GC content of 66.73%. The genome was found to host 3,820 protein-coding sequences, 12 rRNAs, and 55 tRNAs. Intriguingly, annotations derived from the eggNOG and KEGG databases indicate the presence of genes affiliated with phosphorus solubilization and nitrogen fixation, including iscU, glnA, and gltB/D associated with nitrogen fixation, and pqqBC associated with inorganic phosphate dissolution. Several bioactive secondary metabolite genes in the genome, including pqqCDE, phytoene synthase and squalene synthase predicted by antiSMASH. Moreover, we uncovered a complete metabolic pathway for ammonia, suggesting an ammonia-affinity property inherent to Nguyenibacter sp. L1. This study verifies the nitrogen-fixing and phosphate-dissolving abilities of Nguyenibacter sp. L1 at the molecular level through genetic screening and analysis. The insights gleaned from this study offer strategic guidance for future strain enhancement and establish a strong foundation for the potential incorporation of this bacterium into agricultural practices.

Published
2023
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30. A Lysine‐Based System for CO2 Capture and Catalytic Utilization to Produce Formates: Irreproducibility and Conflicting Reactivity.

Author

Wei, Duo, Kotwica, David, Nardi, Giulia, Moazezbarabadi, Ayeshe, Spannenberg, Anke, Ludwig, Ralf, Junge, Henrik, and Beller, Matthias

Subjects
*CARBON sequestration, *FORMATES, *AMINO acids
Abstract

Recently, we have investigated the amino acid L‐lysine promoted CO2 capture and in‐situ hydrogenation to produce formate applying ruthenium‐ and manganese‐based catalysts (Chem. Sci. 2021, 12, 6020, Nature Energy2022, 7, 438). Noteworthy, following this process CO2 can be captured from ambient air and directly converted to formate (TON>50,000). In this Comment, we report how hydrochloride impurities in the amino acid affect the CO2 capture efficiency and therewith lead to an ostensibly different behaviour of D‐ and L‐lysine in the CO2 capture. The results demonstrate the importance of careful analysis of all educts. [ABSTRACT FROM AUTHOR]

Published
2023
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45. Sigma-1 receptor is involved in diminished ovarian reserve possibly by influencing endoplasmic reticulum stress-mediated granulosa cells apoptosis

Author

Shao-di Zhang, Dongjun Fu, Lile Jiang, Jin-Quan Cui, Wei Duo, Cuilian Zhang, and Juan-ke Xie

Subjects
Agonist, Adult, Aging, medicine.medical_specialty, endocrine system, Thapsigargin, medicine.drug_class, Dehydroepiandrosterone, chemistry.chemical_compound, Internal medicine, Intracellular receptor, medicine, Humans, Receptors, sigma, Receptor, Ovarian Reserve, Sigma-1 receptor, Granulosa Cells, Endoplasmic reticulum, fungi, Ovary, apoptosis, Cell Biology, Endoplasmic Reticulum Stress, Endocrinology, chemistry, sigma-1 receptor, Apoptosis, Female, diminished ovarian reserve, Research Paper
Abstract

Sigma non-opioid intracellular receptor 1 (sigma-1 receptor), a non-opioid transmembrane protein, is located on cellular mitochondrial membranes and endoplasmic reticulum. Current research has demonstrated that sigma-1 receptor is related to human degenerative diseases. This study is focused on the effects of sigma-1 receptor on the pathophysiological process of diminished ovarian reserve (DOR) and granulosa cells (GCs) apoptosis. Sigma-1 receptor concentration in follicular fluid (FF) and serum were negatively correlated with basal follicle-stimulating hormone (FSH) and positively correlated with anti-mullerian hormone (AMH), antral follicle count (AFC). Sigma-1 receptor reduction in GCs was accompanied by endoplasmic reticulum stress (ERS)-mediated apoptosis in women with DOR. Plasmid transfection was used to establish SIGMAR1-overexpressed and SIGMAR1-knockdown human granulosa-like tumor (KGN) cell and thapsigargin (TG) was used to induce ERS KGN cells. We found that KGN cells treated with endogenous sigma-1 receptor ligand dehydroepiandrosterone (DHEA) and sigma-1 receptor agonist PRE-084 showed similar biological effects to SIGMAR1-overexpressed KGN cells and opposite effects to SIGMAR1-knockdown KGN cells. DHEA may improve DOR patients' pregnancy outcomes by upregulating sigma-1 receptor and downregulating ERS-mediated apoptotic genes in GCs. Thus, sigma-1 receptor may be a potential ovarian reserve biomarker, and ligand-mediated sigma-1 receptor activation could be a future approach for DOR therapy.

Published
2020

46. Effect of p-GaN Spacer Layer on Emission Spectrum of Yellow-green Dual Wavelength LED

Author

郑畅达 Zheng Chang-da, 王小兰 Wang Xiao-lan, 吴小明 Wu Xiao-ming, 魏铎垒 Wei Duo-lei, 刘军林 Liu Jun-lin, 张建立 Zhang Jian-li, and 江风益 Jiang Feng-yi

Subjects
Radiation, Materials science, business.industry, Optoelectronics, Dual wavelength, Emission spectrum, Condensed Matter Physics, business, Layer (electronics), Electronic, Optical and Magnetic Materials
Published
2020

49. Improved CO2 Capture and Catalytic Hydrogenation Using Amino Acid Based Ionic Liquids.

Author

Moazezbarabadi, Ayeshe, Wei, Duo, Junge, Henrik, and Beller, Matthias

Subjects
CARBON sequestration, IONIC liquids, CATALYTIC hydrogenation, COUNTER-ions, ARGININE
Abstract

A series of alkyl ammonium (or imidazolium) based ionic liquids was tested as novel and potentially green absorbent for CO2 capture and utilization. By exploiting various amino acids as counter ions for ionic liquids, CO2 capture and hydrogenation to formate occur with high activity and excellent productivity utilizing arginine. The reaction was easily scalable without any significant drop in formate production, and the catalyst was reused for five consecutive runs leading to an overall TON of 12,741 for the formation of formate salt. [ABSTRACT FROM AUTHOR]

Published
2022
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