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Supplementary Material 3

Supplementary Material 1

Additional file 1: Supplementary Table S1. Education and work experience of Participants. Supplementary Table S2. Stages and score of emergency rescue procedures for pediatric SE. Supplementary Table S3. The number of times each stage was completed by each group.

In metastasis of cancer cells, the epithelial-mesenchymal transition (EMT) is prerequired. Ferroptosis is an iron-mediated cellular death process, but whether it involves EMT regulation remains elusive. In addition, how stress responders (Nrf2) respond to the redox alteration and cross-talking between them needs to be determined. Our data revealed that DpdtbA (2,2′-di-pyridineketone hydrazone dithiocarbamate butyric acid ester) resisted TGF-β1-induced EMT in gastric cancer lines (SGC-7901 and MGC-823) through ferritinophagy-mediated ROS production. Furthermore, the depletion of Gpx4 and xCT as well as enhanced lipid peroxidation indicated that DpdtbA acted as Erastin did in ferroptosis induction, which thus provided chance to explore the causal relationship between ferroptosis and EMT. Our data illustrated that ferritinophagy-mediated ferroptosis promoted the EMT inhibition. In addition, activated Nrf2 involved the regulation on both ferroptosis and EMT in response to the alteration in the cellular redox environment. In brief, ferritinophagy-mediated ferroptosis and activation of the Keap1/Nrf2/HO-1 pathway were conducive to the EMT inhibition.

[Objectives] To optimize the ethyl acetate impurity removal method for extracting and isolating mangiferin from mango leaves, and provide raw materials and technical support for development and use of mangiferin related products. [Methods] Five steps (material crushing → ethyl acetate impurity removing → concentrated extract washing → extracting with methanol → crystallization and precipitation) were used. The single factor experiment and L9(34) orthogonal experiment was carried out to optimize the process parameters including extraction time, ultrasonic power, extraction times, and extraction temperature. [Results] The optimum process of ethyl acetate impurity removal method for extracting and isolating mangiferin from mango leaves was as follows: the mango leaves were crushed and sieved; 3 mL/g of ethyl acetate was added, sealed and soaked for 4 h, ultrasonically shaken for 20 min (50℃, 350 W), filtered at room temperature, filtered with 100 mesh sieve, and extracted three times; added 100% methanol to the residue at 3 mL/g, extract by ultrasonic vibration for 20 min (350 W, 55℃) for four times, filtered with 100 mesh sieve when it was still hot; mixed the extract of each time, condensed by vacuum decompression to get the extract; added 100% methanol at 4 mL/g, mixed and washed for 5 min at room temperature, placed for 10 min, filtered with 100 mesh sieve, washed 3 times repeatedly, and dried the filter residue at 60℃ to obtain the crude mangiferin; added 100% methanol at 4 mL/g, mixed and washed at 50℃ for 5 min, placed at 6℃ for 8 h, dried the filter residue at 60℃, and repeatedly crystallized two times. According to the above process, crude and pure mangiferin products could be obtained, the purity of mangiferin of the crude product was higher than 64.00%, the total recovery rate was 83.90%, and the purity of mangiferin of the pure product was higher than 98.00%, and the total recovery rate was about 66.40%. [Conclusions] The optimized ethyl acetate impurity removal method is easy in operation, low in cost, and high in efficiency for extracting and isolating mangiferin, and can be applied for actual production of mangiferin.

Supplemental Digital Content is available in the text, Background: Chronic stable angina (CSA) resulted in a considerable burden for both individuals and the society. In this study we aimed to critically evaluate the effectiveness and safety of Compound salvia pellet compared with nitrates in the treatment of Chronic Stable Angina (CSA) pectoris, and to provide more credible evidence for clinical practice. Methods: A comprehensive and exhaustive search strategy was formulated to identify potential RCTs of compound salvia pellet for CSA in international and Chinese databases from their inception to July 4th, 2018. We also searched the bibliographies of relevant studies. Two reviewers independently assessed the quality of included trials by using Cochrane Risk of Bias Tool. Results: The literature search yielded 1849 citations and 51 RCTs (n = 4732) were included for meta-analysis after titles, abstracts and full text selection according to eligibility criteria. The pooled results suggested that compound salvia pellet was much more effective than nitrates in the improvement of angina symptoms (therapy = 4 weeks, RR = 1.23, 95%CI = [1.17, 1.30], P 4 weeks, RR = 1.30, 95%CI[1.20, 1.42], P