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1. Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity

Author

Fei Ma, Chun-yang Zhang, Su Jiang, Wei Tantai, Meng Liu, Qinfeng Xu, and Xiaoran Zou

Subjects
chemistry.chemical_classification, biology, Aptamer, Metals and Alloys, food and beverages, Endogeny, General Chemistry, Histone acetyltransferase, Aptamers, Nucleotide, Molecular biology, Catalysis, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Histones, Enzyme, chemistry, Gene Expression Regulation, Rolling circle replication, Cancer cell, Materials Chemistry, Ceramics and Composites, biology.protein, Histone acetyltransferase activity, Label free, Histone Acetyltransferases
Abstract

We develop for the first time an aptamer-mediated rolling circle amplification approach for label-free and sensitive detection of histone-modifying enzyme (HME) activity. This method can achieve femtomolar sensitivity for histone acetyltransferase Tip60 assay, which is the most sensitive HME assay reported so far. It can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.

Published
2021

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