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4 results on '"Wei-Dih Sy"'

1. Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells

Author

Wei Dih Sy, Chih Hsien Wu, Shyr Ming Sheen-Chen, Chung Ren Lin, Yi Shen Chen, Kuan Hung Chen, Jiin Tsuey Cheng, and Chien Hui Yang

Subjects
Programmed cell death, Cell type, Cell Survival, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Genetic Vectors, Green Fluorescent Proteins, lcsh:Medicine, Biology, Transfection, Viral vector, Dorsal root ganglion, Tubulin, Ganglia, Spinal, medicine, Animals, Pharmacology (medical), Ultrasonics, Molecular Biology, Cells, Cultured, Biochemistry, medical, Neurons, Liposome, Research, lcsh:R, Biochemistry (medical), fungi, Lentivirus, Gene Transfer Techniques, General Medicine, Cell Biology, Molecular biology, Recombinant Proteins, Rats, medicine.anatomical_structure, Liposomes, biology.protein, Sonoporation
Abstract

Background Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. Conclusions Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons.

Published
2010

2. Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells.

Author

Chung-Ren Lin, Kuan-Hung Chen, Chien-Hui Yang, Jiin-Tsuey Cheng, Shyr-Ming Sheen-Chen, Chih-Hsien Wu, Wei-Dih Sy, and Yi-Shen Chen

Subjects
GENETIC transformation, SONICATION, GANGLIA, NEURONS, LIPOSOMES
Abstract

Background: Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods: Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results: The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. Conclusions: Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons. [ABSTRACT FROM AUTHOR]

Published
2010
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3. Altered neuronatin expression in the rat dorsal root ganglion after sciatic nerve transection.

Author

Kuan-Hung Chen, Chien-Hui Yang, Jiin-Tsuey Cheng, Chih-Hsien Wu, Wei-Dih Sy, and Chung-Ren Lin

Subjects
AXONS, GENETIC regulation, GENES, IMMUNOHISTOCHEMISTRY, SCIATIC nerve, HYPERALGESIA
Abstract

Background: Several molecular changes occur following axotomy, such as gene up-regulation and down-regulation. In our previous study using Affymetrix arrays, it was found that after the axotomy of sciatic nerve, there were many novel genes with significant expression changes. Among them, neuronatin (Nnat) was the one which expression was significantly up-regulated. Nnat was identified as a gene selectively expressed in neonatal brains and markedly reduced in adult brains. The present study investigated whether the expression of Nnat correlates with symptoms of neuropathic pain in adult rats with transected sciatic nerve. Methods: Western blotting, immunohistochemistry, and the Randall and Selitto test were used to study the protein content, and subcellular localization of Nnat in correlation with pain-related animal behavior. Results: It was found that after nerve injury, the expression of Nnat was increased in total protein extracts. Unmyelinated C-fiber and thinly myelinated A-δ fiber in adult dorsal root ganglions (DRGs) were the principal subpopulation of primary afferent neurons with distributed Nnat. The increased expression of Nnat and its subcellular localization were related to mechanical hyperalgesia. Conclusions: The results indicated that there was significant correlation between mechanical hyperalgesia in axotomy of sciatic nerve and the increased expression of Nnat in C-fiber and A-δ fiber of adult DRG neurons. [ABSTRACT FROM AUTHOR]

Published
2010
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4. Altered neuronatin expression in the rat dorsal root ganglion after sciatic nerve transection

Author

Chien Hui Yang, Kuan Hung Chen, Chung Ren Lin, Wei Dih Sy, Chih Hsien Wu, and Jiin Tsuey Cheng

Subjects
Male, Pain Threshold, medicine.medical_specialty, medicine.medical_treatment, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, lcsh:Medicine, Gene Expression, Nerve Tissue Proteins, Biology, Nerve Fibers, Myelinated, Rats, Sprague-Dawley, Dorsal root ganglion, Internal medicine, Ganglia, Spinal, Gene expression, Threshold of pain, medicine, Animals, Pharmacology (medical), Molecular Biology, Biochemistry, medical, Nerve Fibers, Unmyelinated, Research, lcsh:R, Biochemistry (medical), Membrane Proteins, Axotomy, General Medicine, Anatomy, Cell Biology, Immunohistochemistry, Sciatic Nerve, Rats, medicine.anatomical_structure, Endocrinology, Hyperalgesia, Neuropathic pain, Neuralgia, Neuronatin, Sciatic nerve, medicine.symptom
Abstract

Background Several molecular changes occur following axotomy, such as gene up-regulation and down-regulation. In our previous study using Affymetrix arrays, it was found that after the axotomy of sciatic nerve, there were many novel genes with significant expression changes. Among them, neuronatin (Nnat) was the one which expression was significantly up-regulated. Nnat was identified as a gene selectively expressed in neonatal brains and markedly reduced in adult brains. The present study investigated whether the expression of Nnat correlates with symptoms of neuropathic pain in adult rats with transected sciatic nerve. Methods Western blotting, immunohistochemistry, and the Randall and Selitto test were used to study the protein content, and subcellular localization of Nnat in correlation with pain-related animal behavior. Results It was found that after nerve injury, the expression of Nnat was increased in total protein extracts. Unmyelinated C-fiber and thinly myelinated A-δ fiber in adult dorsal root ganglions (DRGs) were the principal sub-population of primary afferent neurons with distributed Nnat. The increased expression of Nnat and its subcellular localization were related to mechanical hyperalgesia. Conclusions The results indicated that there was significant correlation between mechanical hyperalgesia in axotomy of sciatic nerve and the increased expression of Nnat in C-fiber and A-δ fiber of adult DRG neurons.

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