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To study the effect of Angelica sinensis polysaccharide (ASP) on the expression of Sirt1/p53/p21 in radiation-induced aging hematopoietic stem cells (HSCs) of mice, and to explore the possible mechanism of ASP regulating the aging of HSCs. Methods: C57BL/6J mice were randomly divided into control group, model group and ASP group. The model group was irradiated with X-ray 3.0Gy/8F to establish the aging model of HSCs in mice. ASP group was given ASP intragastric administration during irradiation; The control group and the model group were given equal volume of normal saline. HSCs were sorted by immunomagnetic beads, and cell cycle was detected by flow cytometry The aging cells were observed by α-galactosidase staining. The directional differentiation ability of HSCs was observed by mixed colony culture (CFU-Mix). The expression of Sirtl, p53 and p21 protein was detected by Western blot. Results: Compared with the control group. X-ray can significantly increase the proportion of HSCs Gi cells, the rate of SA-α-Gal positive cells and the expression of p53 and p21 protein in aging group (PPPP

Consumer behavior, by definition, is concerning with various actions adopted by a person to acquire, use and dispose of consumer goods and services, including the decision-making process that precedes and determines these actions. In real practice, such behavior can be altered with the introduction of various internet-based financing services. Especially for college students, who have relatively weak knowledge of personal finance and insufficient vigilance of excessive consumption, their consumption behavior can be easily manipulated by internet-based financing service providing firms. In this paper, the objective is to determine the dominant factors adopted by internet-based financing service providers to modify the consumption behavior of college students. From 571 college students’ questionnaires, the collected data are subjected to factor and logistic regression analysis to verify what factors are likely to stimulate the willingness of college students to actively employing the internet-based financing services to fulfill their advanced consumption demands. The obtained result indicates the service and convenience factors are likely to influence and alter their consumption behavior, and the corresponding recommendations on how to refine and regulate such market are proposed at the end of this paper.

Soil bacteria play a key role in the plant–soil system and can regulate the growth of Phoebe bournei seedlings under fertilization. However, there are few reports on how soil bacteria respond to fertilization and regulate seedling growth. This study adopted the “3414” field fertilization experiment, combined with soil microbial sequencing, nutrient contents, and biomass measurement, to explore the changes of soil chemical properties and bacterial structure under different NPK fertilization conditions and to establish the coupling relationship between soil bacteria, soil nutrients, and plant growth. The results showed that NPK fertilization decreased soil pH, increased soil N, P, and K content, reduced bacterial diversity and abundance, promoted the growth of dominant bacterial species, and enhanced Phoebe bournei seedlings’ soil N, P, and K elements. NPK fertilization promoted Proteobacteria growth, especially of three genera (Methylobacterium, Sphingobium, and Acinetobacter) and Actinobacteria, while it decreased Acidobacteria and Chloroflexi. By reducing the ratio of N to K and increasing P, NPK fertilization can slow soil acidification, promote bacterial reproduction, maintain P. bournei seedlings’ soil ecological stability, and balance the seedlings’ growth and sustainable soil utilization. AD3, Pseudomonas, and Rhodanobacter can be used as the marker species for N, P, and K fertilization, respectively, while Methylobacterium, Brevundimonas, Acinetobacter, and Sphingobium can be used as indicator species for soil pH and soil N, P, and K content changes, respectively. These results provided a theoretical basis and technical guidance for the effective fertilization and cultivation of robust P. bournei seedlings.

We investigate the effect of ozone (O3) oxidation of silicon carbide (SiC) on the flat-band voltage (V fb) stability of SiC metal–oxide–semiconductor (MOS) capacitors. The SiC MOS capacitors are produced by O3 oxidation, and their V fb stability under frequency variation, temperature variation, and bias temperature stress are evaluated. Secondary ion mass spectroscopy (SIMS), atomic force microscopy (AFM), and x-ray photoelectron spectroscopy (XPS) indicate that O3 oxidation can adjust the element distribution near SiC/SiO2 interface, improve SiC/SiO2 interface morphology, and inhibit the formation of near-interface defects, respectively. In addition, we elaborate the underlying mechanism through which O3 oxidation improves the V fb stability of SiC MOS capacitors by using the measurement results and O3 oxidation kinetics.

The complete chloroplast genome of Cryptomeria japonica var. sinensis Miquel was assembled and analyzed. The chloroplast genome of C. japonica var. sinensis Miquel did not have a typical quadripartite structure with the inverted repeats (IR) absent, and the size of C. japonica var. sinensis is 131,412 bp. The overall GC content was 35.4%. The genome encoded a set of 119 genes, containing 83 protein-coding genes, 32 tRNA genes, and 4 rRNA genes. Phylogenomic analysis indicated that C. japonica var. sinensis is sister to C. japonica (Thunb. ex L. f.) D. Don.

Plagiodera versicolora (Laicharting) is a leaf-eating pest widely distributed in the world. In this study, the first complete mitochondrial genome of P. Versicolora (Laicharting) was assembled and analyzed. The complete mitochondrial genome of P. Versicolora (Laicharting) is 16,857 bp with 22.39% GC containing, 13 protein-coding genes, 22 transfer RNA (tRNA), 2 ribosomal RNA (rRNA), as well as an AT-rich region. Phylogenomic analysis indicated that P. Versicolora (Laicharting) is sister to Chrysomela populiThis study provides useful information for the identification of this species and the study of genetic evolution with other species of Chrysomelidae.

In this study, the first complete mitochondrial genome of Chrysolina aeruginosa Fald was assembled and analyzed. The total length of this mitochondrial genome is 16,335 base pairs. It consists of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and an AT-rich region. Phylogenomic analysis indicated that C. aeruginosa Fald is sister to Chrysodinopsis sp. This study provides new molecular data for the further taxonomic and phylogenetic studies of the Chrysomelidae of Coleoptera.

To explore the regularity of traditional Chinese medicine(TCM) prescriptions for cardio-cerebrovascular diseases,the core drug groups with common therapeutic effects on cerebrovascular diseases represented by stroke and cardiovascular diseases represented by coronary artery disease were extracted,and their consistency and difference in the treatment of different diseases were analyzed.A total of 388 Chinese patent medicines were collected for the treatment of cerebrovascular diseases,cardiovascular diseases and cardio-cerebrovascular diseases.The dominant and recessive patterns of Chinese patent medicines in clinical use were found by "frequency analysis","compatibility analysis" and "network analysis" respectively.According to the findings of the three parts,Salviae Miltiorrhizae Radix et Rhizoma,Chuanxiong Rhizoma,Carthami Flos and Astragali Radix have a high frequency of use in the treatment of brain disease,heart disease and both,with frequent combined medication.Data mining confirmed the core drug combinations for the treatment of cerebral and cardiac vascular diseases,so as to reveal the similarities and differences in the drug use of Chinese medicine for these diseases,and provide a basis for the rational use of traditional Chinese medicine in clinical practice.This analysis also defines a new direction for the future development of prescription combinations for different indications of cerebral and cardiac diseases.

Public Goods Game (PGG) originates from “the Tragedy of Commons”, which is argued by the American scholar Hardin. PGG is a type of evolution game that these individuals in this game can affect each other, and reached a stable state. This paper demonstrates a new version PGG model which is an exclusion PGG model. Besides, combing Parrondo’s Paradox with this PGG model, and it also shows a similar “winning” outcome. Parrondo’s Paradox shows that two individual-losing games, when alternate, yield a winning outcome. Moreover, the additional periodic switching mechanism also applies to the PGG model and obtains a similar result when compared with that of conventional Parrondo’s Paradox. The biggest contribution of this paper is that Parrondo’s Paradox can fully applicable to the exclusion PGG model, and that simulation result also presents an effective result.

Candida haemulonii forms part of the non-albicans Candida species. The candidemia caused by C. haemulonii is extremely rare but fatal in neonates. We reported the first two neonates with C. haemulonii candidemia in China which were treated with fluconazole and reviewed two papers previously reported. Our report adds further awareness on C. haemulonii candidemia in critical neonates and points out the importance of an early diagnosis and treatment of this fatal infection.La Candida haemulonii forma parte de la especie Candida no albicans. La candidemia por C. haemulonii es sumamente infrecuente, pero mortal, en los recién nacidos. Se informa sobre los dos primeros recién nacidos con candidemia por C. haemulonii en China tratados con fluconazol y se revisan dos artículos informados con anterioridad. Nuestro informe incrementa la sensibilización sobre la candidemia por C. haemulonii en recién nacidos críticos y resalta la importancia de un diagnóstico y un tratamiento tempranos de esta infección mortal.

Explosive welding is widely applied to joining Ti with steel in recent years. However, joining the explosion-bonded CP-Ti/Q345 bimetallic sheets has not been thoroughly investigated. In this paper, explosion-bonded CP-Ti/Q345 bimetallic sheets were applied as base metal and intermediate materials were employed to join the bimetallic sheets. Based on the element metallurgical compatibility, Cu/V based filler metals (solid wire and flux-cored wire) were designed as intermediate layers for TIG welding method. To determine the feasibility of these filler metals, microstructures were analyzed by optical microscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD). Microhardness tests were used to compare the brittleness of the intermetallics in the weld joints. Tensile strength test was performed to evaluate the strength of the joint. The results showed that the feasibility of the Cu/V based solid wires was poor due to metallurgical incompatibilities. The joint filled with Cu/V solid wires suffered severe cracks. Such defects reduced if a Cu–V flux-cored wire was used. It was attributed to continuously distributed microstructures in weld joint and low heat input with the Cu–V flux-cored wire.

Objective: To investigate the efficacy, safety, and clinical benefit of prolonged-release trazodone (Trittico) in the treatment of major depressive disorder (MDD). Methods: In this study, 363 Chinese patients with MDD were randomized 1:1 to receive either prolonged-release trazodone (150-450 mg) or placebo treatment for 6 weeks. The primary efficacy measurement was the change of the 17-item Hamilton Depression Rating Scale (HAMD-17) total score from baseline to the end of the study. The secondary efficacy measurements were the response and remission rates, the Clinical Global Impression - Improvement of Illness (CGI-I) score at the end of the study, and the change of the HAMD-14 total score and quality of sleep [evaluated by the Pittsburgh Sleep Quality Index (PSQI) scale] during the study period. Results: The mean maximum daily dose was 273.11 mg for the trazodone group and 290.92 mg for the placebo group. At the end of the study, there was a significant difference between the two groups in the HAMD-17 change score (trazodone vs. placebo: -11.07 vs. -8.29, p < 0.001). Trazodone showed advantages at 1 week of treatment, and the effect lasted until the end of the study (week 6). The response and remission rates of the trazodone group were significantly higher than those in the placebo group (response rate: 59.6 vs. 37.2%, p < 0.001; remission rate: 35.5 vs. 22.2%, p = 0.005). The majority of the adverse reactions of trazodone were mild to moderate, and the most frequent adverse reactions (≥5%) were dizziness, dry mouth, somnolence, and nausea. Conclusions: Prolonged-release trazodone was more effective than placebo in MDD and was well tolerated. i 2014 S. Karger AG, Basel

A quick HPLC-ESI-MS/MS method was established for simultaneous determination of three chemical compositions in Usnea, including usnic acid, diffractaic acid, and ramalic acid. The separation was performed on a chromatographic column of Agilent ZORBAX SB-C, (4.6 mm x 250 mm, 5 µm), and the mobile phase was methanol (0.05% formic acid)-0.05% formic acid solution (4 mmol ammonium acetate), with an isocratic elution at a flow rate of 0.8 ml · min⁻¹. Multiple reaction monitoring scanning mode (MRM) was performed combined with the ion switching technology in positive and negative ion switching mode to apply for the quantitative determination. The calibration curves for the above three compounds were linear in corresponding injection amount. Their average recoveries were 95.0%-105.1%, with RSDs of 1.1%-5.2%. The method was simple, rapid, accurate with high repeatability, which could provide a reference for overcalling evaluation the quality of Usnea.

A newly developed coal flotation collector EDO and its flotation characteristics for three Shanxi different coals were introduced in this paper. EDO was an emulsion composed by diesel oil, water and independent development surfactant PGS. Compared flotation experiments show that, at the same dosage, EDO gives 2~5% combustile material recovery promotion than typical collector with only a slightly ash content increment, but saving over 70% diesel oil, and improves the floatation speed. EDO could promote the dispersion of diesel oil in the slurry, so that the efficiency of floatation was improved. The flotation results showed that the EDO had the characteristics of excellent performance, high selectivity and good stability.

This paper introduced a newly developed coal flotation collector ZFC and its flotation characteristics for Shanxi three different coal samples. ZFC was an emulsion composed by 27% diesel oil, 70% water and 3% homemade surfactant PGS. The surfactant PGS was synthesized with environmental friendly reproducible oil and several surfactants. The flotation results showed that the ZFC had the characteristics of excellent performance, high selectivity and good stability. ZFC could promote the dispersion of diesel oil in the slurry, so that the efficiency of floatation was improved and about 70% diesel oil was saved.

In this paper, the nonlinear matrix equation X + A ∗ X q A = Q ( q > 0) is investigated. Some necessary and sufficient conditions for existence of Hermitian positive definite solutions of the nonlinear matrix equations are derived. An effective iterative method to obtain the positive definite solution is presented. Some numerical results are given to illustrate the effectiveness of the iterative methods.

Using a new type of emulsified flotation reagent ZFC for flotation research of Qianjiaying and Huaixiao coal samples, experimental results show that the reagent has excellent performance, good stability and high selectivity, which promotes the coal flotation. Compared with the flotation with diesel at the similar clean ash content, the ZFC can increase clean coal recovery and combustible material recovery of 0.39%-14.75% and 0.36%-17.52%, respectively. The dosage of ZFC is less than the used diesel oil and the oil saving rate reaches up 70%. The flotation mechanism of ZFC was also studied by measuring the wetting heat and Zeta potential.

To improve slurryability, high power ultrasonic energy was used to treat coal slurry. The influence of power on the slurryability of raw coals and on the maximum hydro-content (MHC) was investigated by measuring the apparent viscosity before and after treatment. The experimental results show that the effect of treatment on sluryability is different for different rank coals under the same treatment conditions. The coals metamorphosed to lower degree are more sensitive to the ultrasonic treatment. The apparent viscosity of slurries from coal metamorphosed to medium degree clearly decreases after the ultrasonic treatment. The ultrasonic treatment makes MHC decrease, which improves the slurryability. For Shenmu or Hebi coal slurry after 3 min. treatment the maximum fixed-viscosity concentration is increased by about 0.4% and 0.3%, respectively, when the ultrasonic power is 100 W. The maximum fixed-viscosity concentration increased 2.4% and 1.7%, respectively, when the ultrasonic power was 300 W, but it decreased by about 0.4% and 0.7%, respectively, when the power was 500 W.

Wei-Wei Xie,1,2,* Lin Zhang,1,* Ren-Rong Wu,1 Yan Yu,3 Jing-Ping Zhao,1 Le-Hua Li1 1Mental Health Institute of the Second Xiangya Hospital, National TechnologyInstitute of Psychiatry, Key Laboratory of Psychiatry and Mental Health of Hunan Province, Central South University, Changsha, Hunan, 2Department of Psychiatry, Ningbo Kangning Hospital, Ningbo, 3People’s Hospital of Hunan Province, Changsha, People’s Republic of China *These authors contributed equally tothis work Background: Human P-glycoprotein encoded by the ATP-binding cassette sub-family B member 1 (ABCB1) gene is expressed in the blood–brain barrier. ABCB1 protects the brain from many drugs and toxins such as glucocorticoids through the efflux pump. Recent evidence suggests that a specific allele of theABCB1gene confers susceptibility to major depressive disorder (MDD) in the Japanese population. The aim of this study was to explore the association ofABCB1gene polymorphisms with MDD in a local Chinese Han population.Methods: Two hundred and ninety-two MDD patients and 208 unrelated individuals were matched by age and sex and examined using a case-control design. Six single nucleotide polymorphisms (SNPs) of theABCB1gene, including rs1045642, rs2032583, rs2032582, rs2235040, rs1128503, and rs2235015, were genotyped by ligase detection reaction and multiplex polymerase chain reaction. Linkage disequilibrium and haplotype analysis were investigated in the two study groups. Results: Significant protection for MDD individuals carrying the TG haplotype of rs1045642–rs2032582 was observed (odds ratio 0.470, 95% confidence interval 0.251–0.897, P=0.01).The rs2032582 (G2677T) and rs1128503 (C1236T) SNPs ofABCB1showed nominal associations with MDD; the other four SNPs of theABCB1gene were not associated with MDD.Conclusion: Chinese individuals carrying the TG haplotype of rs1045642–rs2032582 had a nearly 53% lower risk of developing MDD. To the best of our knowledge, this is the first report to analyze the effect ofABCB1polymorphism on the risk of MDD in a Chinese population. Keywords: major depressive disorder,ABCB1gene, single nucleotide polymorphism, pharmacogenetics

The genetic diversity of picoeukaryotic phytoplankton in the 30 lakes along the middle-lower reaches of the Yangtze River was studied using flow cytometry combined with terminal restriction fragment length polymorphism (T-RFLP). The objectives of this study were to confirm the variance in the genetic diversity of picoeukaryotic phytoplankton among the lakes and to reveal its main affecting factors. Though the lakes investigated tended to or already were eutrophic, T-RFLP profiles of 18S rRNA gene fragments from picoeukaryotic phytoplankton showed high diversity. There were altogether 197 T-RFs detected from the lakes that we investigated, with 89 of them obtained in less than 3 lakes. Cluster analysis indicated their similarity was lower than 80%. Shannon-wiener diversity index showed that mesotrophic lakes contained more diverse picoeukaryotic phytoplankton community than eutrophic lakes. The canonical correspondence analysis revealed that the concentration of the total nitrogen had the most important impact on the picoeukaryotic phytoplankton communities (P =0. 006). The results showed that the genetic diversity of picoeukaryotic phytoplankton in the lakes were of great environmental heterogeneity and closely related to the trophic status. Two CCA axes can only explain 10.0% of the total variance in picoeukaryotic phytoplankton composition, suggesting that the evolution of picoeukaryotic phytoplankton was probably connected to the top-down manipulation as well.

A novel approach based on flow cytometric sorting followed by construction of 18S rRNA clone libraries was used to study the diversity of eukaryotic picophytoplankton of lakes. The composition of eukaryotic picophytoplankton community appeared highly variable in three lakes. Eukaryotic picophytoplankton was dominated by Cryptophyta in the Lake Xuanwu, and was mainly composed of Cryptophyta and Chrysophyta in the Lake Zixia. In the Lake Taihu, four phyla were discovered, including Cryptophyta, Chrysophyta, Bacillariophyta and Chlorophyta. Meanwhile, the diversity of eukaryotic picophytoplankton differed in various lake regions. In the Meiliang Bay, Chrysophyta was the dominant, and the other three phyla were found in the Gonghu Bay. In the central lake, all of those four phyla were discovered, implying this region contained the highest diversity. The canonical correspondence analysis between the diversity of eukaryotic picophytoplankton and environmental factors revealed the concentration of total phosphorus had the highest important impact on the eukaryotic picophytoplankton communities.

Arterial stiffness, considered an independent predictor of cardiovascular morbidity and mortality, is closely associated with hypertension. Futhermore, the role of ghrelin in the development of hypertension has been widely recognized. The purpose of the present study was to explore the potential relationship between circulating ghrelin and arterial stiffness in hypertensive subjects. A total of 192 patients with primary hypertension and 107 normotensive (NT) control subjects were enrolled in the present cross-sectional study. Plasma ghrelin was determined by ELISA. Arterial stiffness was assessed by brachial-ankle pulse wave velocity (baPWV) and the augmentation index (AIx). Both baPWV and AIx values were markedly higher in the hypertensive compared with NT group (P < 0.01). In contrast, plasma ghrelin concentrations were significantly lower in hypertensive patients compared with NT subjects (P < 0.01). Plasma ghrelin concentrations were negatively correlated with age (odds ratio (OR) -1.836; P < 0.001), smoking (OR -1.347; P = 0.042), baPWV (OR -1.762; P < 0.001) and AIx (OR -1.516; P = 0.005), but positively associated with fasting plasma glucose (OR 1.293; P = 0.047) and HbA1c (OR 1.413; P = 0.025). The inverse correlation between circulating ghrelin and the extent of arterial stiffness suggests that ghrelin is an independent determinant of arterial stiffness, even after adjustment for confounding cardiovascular risk factors, and it actively participates in the pathophysiology of arterial stiffness in hypertensive subjects.

Ultrathin VO2 nanobelts with rough alignment features are prepared on the induction layer-coated substrates by an ethylenediaminetetraacetic acid (EDTA)-mediated hydrothermal process. EDTA acts as a chelating reagent and capping agent to facilitate the one-dimensional (1D) preferential growth of ultrathin VO2 nanobelts with high crystallinities and good uniformities. The annealed induction layer and concentration of EDTA are found to play crucial roles in the formation of aligned and ultrathin nanobelts. Variation in EDTA concentration can change the VO2 morphology of ultrathin nanobelts into that of thick nanoplates. Mild annealing of ultrathin VO2 nanobelts at 350 °C in air results in the formation of V2O5 nanobelts with a nearly unchanged ultrathin structure. The nucleation and growth mechanism involved in the formations of nanobelts and nanoplates are proposed. The ethanol gas sensing properties of the V2O5 nanobelt networks-based sensor are investigated in a temperature range from 100 °C to 300 °C over ethanol concentrations ranging from 3 ppm to 500 ppm. The results indicate that the V2O5 nanobelt network sensor exhibits high sensitivity, good reversibility, and fast response-recovery characteristics with an optimal working temperature of 250 °C.