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The human induced pluripotent stem cell (iPSC) line ZJUCHi002-A was established from renal epithelial cells present in urine (urinary cells) collected from an 8-year-old Charcot-Marie-Tooth disease type 2A (CMT2A) patient carrying point mutation in MFN2 (c.752C > T). Urinary cells were reprogrammed by retrovirus vectors containing reprogramming factors: OCT4, SOX2, KLF4 and c-MYC. The pluripotency, capacity of differentiation into 3 germ layers, silence of reprogramming factors and normal karyotype were all confirmed in this study.

The human iPSC cell line ZJUCHi001-A was established from renal epithelial cells present in urine (urinary cells) harvested from a 2-year-old Charcot-Marie-Tooth disease type 1B (CMT1B) patient carrying point mutation in MPZ (c.292C>T). Urinary cells were reprogrammed by retrovirus vectors containing reprogramming factors: OCT4, SOX2, KLF4 and c-MYC. The pluripotency, capacity of differentiation into 3 germ layers, silence of reprogramming factors and normal karyotype for this cell line were all confirmed in this study.

Mass Customization (MC) is a flexible manufacturing system with features of Mass Production (MP) and Customization Production (CP). However, there is few researches about competition & cooperation between the upstream MP firm (module manufacturer) and downstream CP firm (assembler) under MC supply chain scenario. From supply chain perspective, this paper first develops the base models considering the influences of return policy, modularity level, production lead time and pricing factors. Furthermore, according to the different decision-making situations, three kinds of MC supply chain models in competitive or cooperative environment (i.e. simultaneous-move game, sequential-move game and the cooperative game) have been built, then, the optimal solution of each model have been analyzed and compared, and coordination mechanism is design to cooperate in MC supply chain via profit-sharing with Nash bargaining power. Finally, through the numerical analysis, we find the highest profit is from the cooperative setting, then followed by in simultaneous-move and sequential-move one, the reason is that the lowest product price and the largest market demand easily occurs in the cooperative game compared with the others, the upstream module manufacturer takes advantage of MP to increase the modularity level and decrease manufacturing cost for the whole supply chain, the downstream assembler task is to shorten the lead time according to customer’s needs, while the wholesale price in cooperative game higher than simultaneous-move game and sequential-move game can ensure each firm’s benefits, effectively prevent from the effect of double marginalization and obtain Pareto optimality.

Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase catalyzes the transfer of electrons from NADPH to O2, which is the main source of reactive oxygen species (ROS) in nonphagocytic cells. Excess ROS are toxic; therefore, keeping ROS in homeostasis in cells can protect cells from oxidative damage. It is meaningful to further understand the molecular mechanism by which ROS homeostasis is mediated. Human protein HSCARG is a newly identified oxidative sensor and a negative regulator of NF-κB. Here, we find that HSCARG represses the cellular ROS generation through inhibiting mRNA and protein expression of p47phox, a subunit of NADPH oxidase. In contrast, shRNA-mediated HSCARG knockdown increases endogenous p47phox expression level. And HSCARG has no obvious effect on ROS production in p47phox-depleted cells. Furthermore, HSCARG regulates p47phox through inhibition of NF-κB activity. Our findings identify HSCARG as a novel regulator in regulation of the activity of NADPH oxidase and ROS homeostasis.