188 results on '"Weihong He"'
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2. Age-related promoter-switch regulates Runx1 expression in adult rat hearts
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Jiawei Song, Xiaoling Zhang, Sinan Lv, Meng Liu, Xing Hua, Limin Yue, Si Wang, and Weihong He
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Runx1 ,Cardiovascular diseases ,Cardiac ageing ,Transcriptional regulation ,DNA methylation ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background Runt-related transcription factor-1 (RUNX1), a key member of the core-binding factor family of transcription factors, has emerged as a novel therapeutic target for cardiovascular disease. There is an urgent need to fully understand the expression pattern of Runx1 in the heart and the mechanisms by which it is controlled under normal conditions and in response to disease. The expression of Runx1 is regulated at the transcriptional level by two promoters designated P1 and P2. Alternative usage of these two promoters creates differential mRNA transcripts diversified in distribution and translational potential. While the significance of P1/P2 promoter-switch in the transcriptional control of Runx1 has been highlighted in the embryogenic process, very little is known about the level of P1- and P2-specific transcripts in adult hearts, and the underlying mechanisms controlling the promoter-switch. Methods To amplify P1/P2 specific sequences in the heart, we used two different sense primers complementary to either P1 or P2 5'-regions to monitor the expression of P1/P2 transcripts. DNA methylation levels were assessed at the Runx1 promoter regions. Rats were grouped by age. Results The expression levels of both P1- and P2-derived Runx1 transcripts were decreased in older rats when compared with that in young adults, paralleled with an age-dependent decline in Runx1 protein level. Furthermore, older rats demonstrated a higher degree of DNA methylation at Runx1 promoter regions. Alternative promoter usage was observed in hearts with increased age, as reflected by altered P1:P2 mRNA ratio. Conclusion Our data demonstrate that the expression of Runx1 in the heart is age-dependent and underscore the importance of gene methylation in the promoter-mediated transcriptional control of Runx1, thereby providing new insights to the role of epigenetic regulation in the heart.
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- 2023
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3. Application Value of Segond Fracture Imaging Features in Lateral Knee Instability
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Weihong HE, Xi FU, Tingsong FANG, Guining WANG, and Juan WANG
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magnetic resonance imaging ,knee joint ,anterolateral ligament ,injury ,Geophysics. Cosmic physics ,QC801-809 ,Medicine (General) ,R5-920 - Abstract
Objective: To evaluate the imaging features of the Segond fracture and explore its clinical value in lateral instability of the knee joint. Methods: We retrospectively analyzed 45 cases of the Segond fracture. This involved the analysis of the characteristics of the fracture, including the relationship between the fracture and the ligaments around the knee joint, meniscus injury, and the size and location of the fracture block. Moreover, the significance of the Segond fracture in clinical treatment was discussed. Results: Among 45 cases of the Segond fracture, 41 (91.11%) had anterior cruciate ligament (ACL) injury, 31 (68.89%) had anterolateral ligament (ALL) injury, and 12 (26.67%) had iliotibial bundle (ITB) injury. The ALL were all attached to the fracture block, and 25 cases (55.55%) were attached to the ITB. The larger the size of the fracture block and the smaller the distance from the center of Gerdy's nodule, the more likely that the fracture block was attached to the ITB. Conclusion: The Segond fracture is often associated with ACL injury, involving the ALL and ITB. The larger the fracture block and the closer to the center of Gerdy's nodule, the greater the possibility of ITB involvement. Therefore, patients with Segond fractures should be alert to lateral instability of the knee joint.
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- 2023
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4. Regeneration of Sesuvium portulacastrum through Indirect Shoot Organogenesis and Influence of an Endophytic Fungus on Rooting of Microshoots
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Xiuli Jiang, Dan Wang, Jianjun Chen, Weihong He, Boya Zhou, Ziling Li, Lingyan Chen, Donghui Peng, Qiang Chen, and Xiangying Wei
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adventitious shoots ,callus induction ,microshoots ,plant growth-promoting fungi ,plant regeneration ,Sesuvium portulacastrum L. ,Agriculture (General) ,S1-972 - Abstract
Sesuvium portulacastrum L. is a dicotyledonous halophyte belonging to the family Aizoaceae. Its young leaves are highly nutritious, and many ecotypes are used as leafy vegetable and medicinal crops. Additionally, due to their tolerance to soil salinity, flooding, and high temperatures, some ecotypes are used for the remediation of saline soils. As a result, there is an increasing need for a large number of disease-free S. portulacastrum propagules. This study developed an efficient protocol for the regeneration of S. portulacastrum through indirect shoot organogenesis. Leaf explants were cultured on Murashige and Skoog basal medium supplemented with different concentrations of zeatin (ZT) and indole-3-acetic acid (IAA). Callus was induced in all explants cultured with 1.5 mg/L ZT only or 1.5 mg/L ZT with 0.5 mg/L IAA. The callus was cut into small pieces and cultured on the same medium on which it was initially induced. ZT at 1.5 mg/L induced 73.7% of callus pieces to produce adventitious shoots, and the shoot numbers per callus piece were up to 20. To improve the in vitro rooting of adventitious shoots, commonly known as microshoots or microcuttings, an endophytic fungus, Cladosporium ‘BF-F’, was inoculated onto the rooting medium. ‘BF-F’ substantially enhanced rooting and plantlet growth, as the root numbers were three times more and plantlet heights were 70% greater than those without ‘BF-F’ inoculation. To detect the genes involved in the enhanced rooting and plantlet growth, qRT-PCR analysis was performed. Results showed that genes related to auxin responses and nitrogen uptake and metabolism were highly upregulated in ‘BF-F’-inoculated plantlets. Plants inoculated with ‘BF-F’ grew vigorously after being transplanted into a sand–soil substrate. Thus, this study not only established an efficient protocol for the regeneration of S. portulacastrum but also developed a novel method for improving the rooting of microshoots and plantlet growth. The established propagation system could be used for producing a large number of S. portulacastrum plantlets for commercial use and also for genetic transformation.
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- 2024
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5. Risk Factors of Vulnerable Coronary Plaque Formation in Type 2 Diabetes
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Weihong HE, Tingsong FANG, Xi FU, Meiling LAO, and Xiuyun XIAO
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type 2 diabetes ,vulnerable plaque ,ct angiography ,Geophysics. Cosmic physics ,QC801-809 ,Medicine (General) ,R5-920 - Abstract
Objective: To explore the related factors of coronary vulnerable plaque in type 2 diabetes (T2DM) and to provide an important basis for clinical prediction and management of coronary vulnerable plaque in patients with T2DM. Methods: A retrospective analysis of 150 patients was performed using T2DM-related clinical indicators and coronary plaque CT angiography (CTA) data, and by the presence of vulnerable plaque groups, to explore the independent risk factors of coronary vulnerable plaque in patients with T2DM and related factors of the ROC curve analysis of its diagnostic efficacy. Results: TG was an independent risk factor for vulnerable plaque in T2DM cases (OR=1.49, 95% CI 1.02~2.18); TIR (OR=0.95, 95% CI 0.92~0.97) and HDL (OR=0.32, 95% CI 0.13~0.78) were independent protection factors. The AUC of TIR, TG, and HDL was 0.71, 0.69, and 0.65, respectively. The AUC for joint prediction was 0.76, 95% CI was 0.68~0.83, sensitivity was 75%, and specificity was 70%. Conclusions: When TIR and HDL decrease and TG increases in patients with T2DM, clinicians should be alert to the possibility of a coronary vulnerable plaque.
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- 2023
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6. A novel endophytic fungus strain of Cladosporium: its identification, genomic analysis, and effects on plant growth
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Nan Yang, Wenbin Zhang, Dan Wang, Dingding Cao, Yanyu Cao, Weihong He, Ziting Lin, Xiaofeng Chen, Guiping Ye, Zhiming Chen, Jianjun Chen, and Xiangying Wei
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Cladosporium ,genome sequencing ,IAA ,plant growth-promoting fungi (PGPF) ,BF-F ,PGP gene clusters ,Microbiology ,QR1-502 - Abstract
IntroductionEndophytic microorganisms are bacteria or fungi that inhabit plant internal tissues contributing to various biological processes of plants. Some endophytic microbes can promote plant growth, which are known as plant growth-promoting endophytes (PGPEs). There has been an increasing interest in isolation and identification of PGPEs for sustainable production of crops. This study was undertaken to isolate PGPEs from roots of a halophytic species Sesuvium portulacastrum L. and elucidate potential mechanisms underlying the plant growth promoting effect.MethodsSurface-disinfected seeds of S. portulacastrum were germinated on an in vitro culture medium, and roots of some germinated seedlings were contaminated by bacteria and fungi. From the contamination, an endophytic fungus called BF-F (a fungal strain isolated from bacterial and fungal contamination) was isolated and identified. The genome of BF-F strain was sequenced, its genome structure and function were analyzed using various bioinformatics software. Additionally, the effect of BF-F on plant growth promotion were investigated by gene cluster analyses.ResultsBased on the sequence homology (99%) and phylogenetic analysis, BF-F is likely a new Cladosporium angulosum strain or possibly a new Cladosporium species that is most homologous to C. angulosum. The BF-F significantly promoted the growth of dicot S. portulacastrum and Arabidopsis as well as monocot rice. Whole genome analysis revealed that the BF-F genome has 29,444,740 bp in size with 6,426 annotated genes, including gene clusters associated with the tryptophan synthesis and metabolism pathway, sterol synthesis pathway, and nitrogen metabolism pathway. BF-F produced indole-3-acetic acid (IAA) and also induced the expression of plant N uptake related genes.DiscussionOur results suggest that BF-F is a novel strain of Cladosporium and has potential to be a microbial fertilizer for sustainable production of crop plants. The resulting genomic information will facilitate further investigation of its genetic evolution and its function, particularly mechanisms underlying plant growth promotion.
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- 2023
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7. Association between serum NLRP3 and malignant brain edema in patients with acute ischemic stroke
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Yanan Wang, Hexiao Huang, Weihong He, Shihong Zhang, Ming Liu, and Simiao Wu
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NLRP3 ,Malignant brain edema ,Acute ischemic stroke ,Biomarkers ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background We aimed to explore the association of serum level of the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) and its related inflammatory biomarkers (hypoxia inducible factor-1α, cathepsin B, caspase-1 and matrix metalloproteinase-9) with malignant brain edema (MBE) in patients with acute ischemic stroke. Methods We prospectively enrolled patients with acute ischemic stroke admitted
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- 2021
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8. A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper
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Shaoliang Mou, Qianqian Meng, Feng Gao, Tingting Zhang, Weihong He, Deyi Guan, and Shuilin He
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Capsicum annuum ,CaCRK5 ,Ralstonia solanacearum ,Immune response ,Botany ,QK1-989 - Abstract
Abstract Background Cysteine-rich receptor-like kinases (CRKs) represent a large subfamily of receptor-like kinases and play vital roles in diverse physiological processes in regulating plant growth and development. Results CaCRK5 transcripts were induced in pepper upon the infection of Ralstonia solanacearum and treatment with salicylic acid. The fusions between CaCRK5 and green fluorescence protein were targeted to the plasma membrane. Suppression of CaCRK5 via virus-induced gene silencing (VIGS) made pepper plants significantly susceptible to R. solanacearum infection, which was accompanied with decreased expression of defense related genes CaPR1, CaSAR8.2, CaDEF1 and CaACO1. Overexpression of CaCRK5 increased resistance against R. solanacearum in Nicotiana benthamiana. Furthermore, electrophoretic mobility shift assay and chromatin immunoprecipitation coupled with quantitative real-time PCR analysis revealed that a homeodomain zipper I protein CaHDZ27 can active the expression of CaCRK5 through directly binding to its promoter. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) analyses suggested that CaCRK5 heterodimerized with the homologous member CaCRK6 on the plasma membrane. Conclusions Our data revealed that CaCRK5 played a positive role in regulating immune responses against R. solanacearum infection in pepper.
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- 2021
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9. Transcriptome analysis reveals molecular mechanisms underlying salt tolerance in halophyte Sesuvium portulacastrum
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Dan Wang, Nan Yang, Chaoyue Zhang, Weihong He, Guiping Ye, Jianjun Chen, and Xiangying Wei
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salt tolerance ,Sesuvium portulacastrum ,transcriptomic analysis ,differentially expressed genes ,ion transport ,Plant culture ,SB1-1110 - Abstract
Soil salinity is an important environmental problem that seriously affects plant growth and crop productivity. Phytoremediation is a cost-effective solution for reducing soil salinity and potentially converting the soils for crop production. Sesuvium portulacastrum is a typical halophyte which can grow at high salt concentrations. In order to explore the salt tolerance mechanism of S. portulacastrum, rooted cuttings were grown in a hydroponic culture containing ½ Hoagland solution with or without addition of 400 mM Na for 21 days. Root and leaf samples were taken 1 h and 21 days after Na treatment, and RNA-Seq was used to analyze transcript differences in roots and leaves of the Na-treated and control plants. A large number of differentially expressed genes (DEGs) were identified in the roots and leaves of plants grown under salt stress. Several key pathways related to salt tolerance were identified through KEGG analysis. Combined with physiological data and expression analysis, it appeared that cyclic nucleotide gated channels (CNGCs) were implicated in Na uptake and Na+/H+ exchangers (NHXs) were responsible for the extrusion and sequestration of Na, which facilitated a balance between Na+ and K+ in S. portulacastrum under salt stress. Soluble sugar and proline were identified as important osmoprotectant in salt-stressed S. portulacastrum plants. Glutathione metabolism played an important role in scavenging reactive oxygen species. Results from this study show that S. portulacastrum as a halophytic species possesses a suite of mechanisms for accumulating and tolerating a high level of Na; thus, it could be a valuable plant species used for phytoremediation of saline soils.
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- 2022
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10. Trophic structure of fishes and macroinvertebrates in relation to environmental indicators in artificial reef ecosystems of Pearl River Estuary
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Weiguo Zhou, Peng Xu, Dewen Ding, Anning Suo, Mujiao Xie, Li Zhang, Weizhong Yue, and Weihong He
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Artificial reef ,Stable isotopes ,Functional groups ,Trophic structure ,Trophic pathway ,Environmental indicators ,Ecology ,QH540-549.5 - Abstract
Artificial reefs are frequently deployed in marine ecosystems to increase fishery yields and enhance the production of reef-associated fauna. However, information regarding trophic structures and pathways of faunal assemblages in artificial reef ecosystems in Pearl River Estuary were still in scare. To this end, we compared the abundances and isotopes of functional groups of fishes and macroinvertebrates in three offshore islands, including Wanshan Island with shallow artificial reefs, Miaowan Island with deep artificial reefs, and Wailingding Island with no reef. The results showed that high connection between pelagic and benthic origin in Wanshan and Wailingding Islands, with pelagic production dominating in Wanshan Island and benthic production dominating in Wailingding Island, respectively. The δ13C value of fishes was significantly lower in Wanshan Island compared to Miaowan Island and Wailingding Island, whilst the δ 15N value of macroinvertebrates was highest in Miaowan Island. Based on the trophic niches, Wanshan Island displayed higher trophic diversity and lower trophic redundancy in fish assemblages, while Miaowan Island was the same in macroinvertebrate assemblages. In addition, four key environmental indicators (i.e., salinity, pH, phosphate, and chemical oxygen demand) and five key environmental indicators (i.e., depth, dissolved oxygen, chlorophyll a, total phosphate, and transparency) were the best at explaining the niche variation of fishes and macroinvertebrates, respectively. Our findings provide an understanding of ecosystem functioning within and around the artificial reef systems to further enhance effective management.
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- 2022
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11. Reconstructed ocean plate stratigraphy sequences from the Permian subduction-accretionary complex in the Qinghai-Tibet Plateau
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Kexin Zhang, Jiaxuan Wang, Weihong He, Shengdong Wang, Yadong Xu, Xionghua Zhang, Bowen Song, Xiaohu Kou, and Guocan Wang
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Subduction-accretionary complex ,Permian ,Ocean plate stratigraphy ,Sequence reconstruction ,Qinghai-Tibet Plateau ,Physical geography ,GB3-5030 - Abstract
The Permian Tethys subduction-accretionary complex (SAC) in the Qinghai-Tibet Plateau (QTP) is distributed in six geotectonic provinces, which are known as the Zongwulong-Ganjia, the Western Kunlun, the Eastern Kunlun, the Ganzi-Litang, the Xijinwulan-Jinshajiang-Ailaoshan and the Longmucuo-Shuanghu-Nujiang from north to south. The SAC is a set of mélange generated by subduction of oceanic crust at trenches, which consists of relics from mid-ocean ridge, abyssal plains, oceanic island-seamounts, intra-oceanic arcs and trench turbidites. In this paper, we apply the ocean plate stratigraphy (OPS) theory to reconstruct the OPS sequence of the Permian SAC in the QTP. We divided the Permian SAC in the QTP into two types, i.e., convergent zone and accretionary zone according to their tectonic environments. Six relics from mid-ocean ridge, five relics from abyssal plain, five relics from seamount, two relics from intra-oceanic arc, two relics from fore-arc trench, and seven relics from continental fore-arc trench were recognized and then the OPS sequences from different tectonic environments were reconstructed. The spatial-temporal distributions and evolution of ocean basins during the Permian represented by six SACs are discussed.
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- 2022
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12. New Permian radiolarians from east Asia and the quantitative reconstruction of their evolutionary and ecological significances
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Yifan Xiao, Noritoshi Suzuki, Tsuyoshi Ito, and Weihong He
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Medicine ,Science - Abstract
Abstract The biostratigraphically important Permian radiolarian genera Pseudoalbaillella sensu stricto and Follicucullus (Follicucullidae, Polycystinea) are discriminated by morphological gaps in their wings and segmentation. Previous statistical analyses demonstrated that Longtanella fills morphological gaps between these two genera. Longtanella has long been regarded as a junior synonym of Parafollicucullus, and only a few species have been described. Herein several true Longtanella species are recognized from South China, and eight new species and five indeterminate species are described and illustrated to prove the validity of the genus Longtanella. In addition, a new genus, Parafollicucullinoides gen. nov., is described. Their palaeogeographic distributions and living environments are explored by applying correspondence analysis (CA), with occurrence datasets of selected fusulinacean genera from the Japanese Islands, China and Sundaland. CA results indicate that Longtanella was present to a limited extent in warmer conditions in the fusulinacean Province B and C during Kungurian–Roadian time, and possibly lived above the thermocline and below the deepest limit of fusulinaceans. The Pseudoalbaillella and the Follicucullus group preferred open ocean conditions, living below the thermocline and distributed not only in the ‘Equatorial Warm Water Province’, but also the northern peri-Gondwana Cool Water Province and the southern North Cool Water Province.
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- 2021
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13. CaLRR-RLK1, a novel RD receptor-like kinase from Capsicum annuum and transcriptionally activated by CaHDZ27, act as positive regulator in Ralstonia solanacearum resistance
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Shaoliang Mou, Feng Gao, Lei Shen, Sheng Yang, Weihong He, Wei Cheng, Yang Wu, and Shuilin He
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CaLRR-RLK1 ,CaHDZ27 ,Pepper ,Ralstonia solanacearum ,Plant immunity ,Botany ,QK1-989 - Abstract
Abstract Background Bacterial wilt caused by Ralstonia solanacearum is one of the most important diseases in pepper worldwide, however, the molecular mechanism underlying pepper resistance to bacterial wilt remains poorly understood. Results Herein, a novel RD leucine-rich repeat receptor-like kinase, CaLRR-RLK1, was functionally characterized in immunity against R. solanacearum. CaLRR-RLK1 was targeted exclusively to plasma membrane and was up-regulated by R. solanacearum inoculation (RSI) as well as by the exogenous application of salicylic acid (SA), methyl jasmonate (MeJA) or ethephon (ETH). The silencing of CaLRR-RLK1 led to enhanced susceptibility of pepper plants to RSI, accompanied by down-regulation of immunity-related genes including CaACO1, CaHIR1, CaPR4 and CaPO2. In contrast, transient overexpression of CaLRR-RLK1 triggered hypersensitive response (HR)-like cell death and H2O2 accumulation in pepper leaves, manifested by darker trypan blue and DAB staining respectively. In addition, the ectopic overexpression of CaLRR-RLK1 in tobacco plants enhanced resistance R. solanacearum, accompanied with the immunity associated marker genes including NtPR2, NtPR2, NtHSR203 and NtHSR515. Furthermore, it was found that CaHDZ27, a positive regulator in pepper response to RSI in our previous study, transcriptionally activated CaLRR-RLK1 by direct targeting its promoter probably in a CAATTATTG dependent manner. Conclusion The study revealed that CaLRR-RLK1 confers pepper resistance to R. solanacearum as the direct targeting of CaHDZ27.
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- 2019
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14. Sesuvium portulacastrum-Mediated Removal of Nitrogen and Phosphorus Affected by Sulfadiazine in Aquaculture Wastewater
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Chaoyue Zhang, Dan Wang, Weihong He, Hong Liu, Jianjun Chen, Xiangying Wei, and Jingli Mu
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aquaculture wastewater ,nitrogen ,phosphors ,rhizosphere microorganisms ,Sesuvium portulacastrum ,sulfadiazine ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Plant-based removal of nitrogen (N) and phosphorus (P) from water bodies is an important method for remediation of aquaculture wastewater. In order to acquire knowledge as to how antibiotic residues in wastewater might affect the microbial community and plant uptake of N and P, this study investigated N and P removal by a coastal plant Sesuvium portulacastrum L. grown in aquaculture wastewater treated with 0, 1, 5, or 50 mg/L sulfonamide antibiotics (sulfadiazine, SD) for 28 days and compared the microbial community structure between the water and rhizosphere. Results showed that SD significantly decreased N removal rates from 87.5% to 22.1% and total P removal rates from 99.6% to 85.5%. Plant fresh weights, root numbers, and moisture contents as well as activities of some enzymes in leaves were also reduced. SD changed the microbial community structure in water, but the microbial community structure in the rhizosphere was less affected by SD. The microbial diversity in water was higher than that in the rhizosphere, indicating microbial community differences. Our results showed that the commonly used antibiotic, SD, in aquaculture can inhibit plant growth, change the structure of microbial community, and reduce the capacity of S. portulacastrum plants to remove N and P from wastewater, and also raised alarm about detrimental effects of antibiotic residues in phytoremediation of wastewater.
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- 2022
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15. In Vitro Shoot Culture of Sesuvium portulacastrum: An Important Plant for Phytoremediation
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Weihong He, Dan Wang, Nan Yang, Dingding Cao, Xiaofeng Chen, Jianjun Chen, and Xiangying Wei
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axillary shoots ,disease-free plants ,in vitro rooting ,micropropagation ,phytoremediation ,Sesuvium portulacastrum L. ,Agriculture (General) ,S1-972 - Abstract
Sesuvium portulacastrum L., a member of the family Aizoaceae, is an important coastal halophyte. Due to its adaptability to salinity and heavy metals, S. portulacastrum has now been widely used for the phytoremediation of saline soils and wastewater and the protection of the coast from erosion. The increasing use of this plant requires a large number of propagules. Stem cutting propagation and seed germination cannot meet this demand, and such propagations can initiate and spread diseases. A recent occurrence of Bipolaris sesuvii J.Z. Zhang and Gibbago trianthemae E.G. Simmons in S. portulacastrum resulted in the substantial loss of the plants during the remediation of aquaculture wastewater. Thus, there is an urgent need for establishing efficient methods of propagating disease-free starting materials. In the present study, we evaluated different growth regulators in the induction of axillary shoots from nodal explants cultured on Murashige and Skoog medium and identified that zeatin (ZT) and α-naphthaleneacetic acid (NAA) was an appropriate combination for inducing high numbers of axillary shoots. The nodal explants were then cultured on MS medium supplemented with different concentrations of ZT and NAA, and the combination of ZT at 1.0 mg L−1 and NAA at 0.3 mg L−1 induced more than 12 axillary shoots per explant. The axillary shoots were excised to produce microcuttings or microshoots, which were rooted on half-strength MS medium supplemented with different concentrations of indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA). The results showed that IBA at 0.6 mg L−1 induced 91.7% of the microcuttings to root with root numbers of over 36 per cutting. The rooted plantlets were healthy and true-to-type and grew vigorously in plug trays or plastic containers with a 100% survey rate in a greenhouse. Thus, this established protocol could be used for the rapid propagation of genetically identical and disease-free plants of S. portulacastrum for phytoremediation and the protection of shoreline soils from erosion.
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- 2021
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16. Applications and limitations of micro-XCT imaging in the studies of Permian radiolarians: A new genus with bi-polar main spines
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Yifan Xiao, Noritoshi Suzuki, and Weihong He
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Radiolaria ,taxonomy ,micro-XCT ,Permian ,Changhsingian ,China ,Fossil man. Human paleontology ,GN282-286.7 ,Paleontology ,QE701-760 - Abstract
Microfocus X-ray Computed Tomography (micro-XCT) has been employed recently in radiolarian studies, though so far primarily to generate high quality tomographic images. Although micro-XCT technique cannot always produce high-quality tomographic images, it frequently can provide valuable information on the internal structure of spongy polycystines. Here we employ micro-XCT to understand internal skeletal structures of several Permian specimens of polycystine radiolarians. Structural inferences from micro-XCT images are compared to images of the same specimens made with SEM and transmitted light microscopy (TLM). The utility of micro-XCT for imaging internal structures is first confirmed by examining the spongy, flat, four-spined species Tetraspongodiscus stauracanthus. Micro-XCT method is then used to examine the internal structures of a spherical to elliptical polycystine with two bi-polar main spines, Dalongicaepa bipolaris Xiao and Suzuki gen. et sp. nov., from the Dalong Formation (Changhsingian) of South China. The new genus is characterized by four to seven densely concentric shells with a large spherical hollow in the center and two cylindrical spines at both poles of the cortical shell, and belongs to the family Spongotortilispinidae. Spherical to elliptical polycystines with bi-polar main spines are similar in external appearance, and their phylogenetic relationships are only determinable by examination of the internal structures. We therefore analyzed all Permian and Mesozoic spherical to elliptical polycystines with bi-polar main spines showing internal structures, using cluster analysis to measure similarity. The results show distinctive differences in internal structures and suggest that family level relationships should be revised in the future.
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- 2017
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17. Seasonal Variation of Dissolved Oxygen in the Southeast of the Pearl River Estuary
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Guangping Liu, Weihong He, and Shuqun Cai
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dissolved oxygen (DO) ,hypoxia ,Pearl River Estuary ,Hydraulic engineering ,TC1-978 ,Water supply for domestic and industrial purposes ,TD201-500 - Abstract
Dissolved oxygen (DO) concentration in estuaries is highly variable at different spatial and temporal scales, which is affected by physical, chemical and biological processes. This study analyzed the spatial–temporal distributions of dissolved oxygen concentration and bottom hypoxia in the southeast of the Pearl River Estuary (PRE) using monthly water quality monitoring and hydrographic data covering the period 2000–2017. The seasonal spatial–temporal variation of DO concentration was studied using various methods, such as rotated empirical orthogonal functions, harmonic analysis, and correlation analysis. The results showed that DO stratification was significant in summer, but it was not distinct in winter, during which DO concentration peaked. DO stratification exhibited a significantly positive correlation with water stratification. In the south and west of Hong Kong (SHK and WHK, respectively), DO concentration fields exhibited distinct seasonal changes in the recent 18 years. In SHK, the main periods of the surface DO variation were 24, 12, and 6 months, whereas the main period was 12 months in WHK. The main period of the bottom DO variation was 12 months in both SHK and WHK. In SHK, the spatial–temporal variations in surface and bottom DO were highly related to the variations of salinity, dissolved inorganic nitrogen (DIN), and active phosphorus, and the variation of surface DO was also connected to the variation of temperature and chlorophyll a. In WHK, the variations in surface and bottom DO were highly related to the variations of salinity and temperature, and the variation of surface DO was also connected to the variation of DIN. The river discharge and wind had a different important influence on the temporal variability of DO in WHK and SHK. These findings suggested that the variations of DO may be controlled by coupled physical and biochemical processes in the southeast of PRE. From 2000 to 2017, bottom hypoxia in the southeast of PRE occurred in the summers of 7 years. SHK appeared to be more vulnerable to hypoxia than WHK.
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- 2020
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18. Two New Coumarins from Micromelum falcatum with Cytotoxicity and Brine Shrimp Larvae Toxicity
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Si Zhang, Yongzhong Huang, Qiao Liu, Qingxin Li, Hao Yin, Xiongming Luo, and Weihong He
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Micromelum falcatum ,coumarins ,brine shrimp larvae toxicity ,cytotoxicity ,antibacterial activity ,Organic chemistry ,QD241-441 - Abstract
Two new coumarins, 7-methoxy-8-(2-hydroxmethyl-1-O-isovaleryl-4-butenyl)-coumarin (1) and 7-methoxy-8-(1-hydroxy-2-O-b-glucopyranosyl-3-methyl-4-butene-1-yl)coumarin (2), and twelve known coumarins 3–14 were isolated from the stem bark of Micromelum falcatum. The structures of compounds 1–14 were elucidated by extensive spectroscopic data analyses. The toxicity of compounds 1–14 was tested using a brine shrimp assay and in vitro antiproliferative assay against mammary cancer (F10) and lung cancer (HvEvc) cell lines by the MTT method. Some compounds had moderate activities. All compounds were also tested against the microorganisms Bacillus subtilis, Bacillus thuringiensis and Escherichia coli, but no activity was observed.
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- 2012
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19. A New Prenylated Flavanone from Derris trifoliata Lour.
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Hao Yin, Si Zhang, Zhihui Xiao, Ximin Qiu, Cheng Jiang, Xiongming Luo, Weihong He, and Shengzi Liu
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Derris trifoliate ,aerial part ,prenylated flavanone ,rotenoids ,brine shrimp toxicity ,Organic chemistry ,QD241-441 - Abstract
A new flavanone, 4′,5,7-trihydroxy-6,8-di-(2-hydroxy-3-methylbut-3-enyl)- flavanone, was isolated from the aerial parts of Derris trifoliate, together with eleven known compounds: rotenone, tephrosin, 12a-hydroxyrotenone, deguelin, 6a,12a-dehydro-rotenone, dehydrodeguelin, 7a-O-methyldeguelol, 7a-O-methylelliptonol, 5,7,3',4'-tetra-hydroxy-6,8-diprenylisoflavone, daidzein and 4'-hydroxy-7-methoxyflavanone. 7a-O-Methylelliptonol was isolated for the first time from the genus Derris. Their structures were characterized on the basis of spectral data. Eight of the isolated compounds were found to be significantly toxic to brine shrimp (LC50 range 0.06–9.95 μg/mL). The new compound showed weak toxicity (LC50 = 211.31 μg/mL).
- Published
- 2012
- Full Text
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20. Identifying Natural syNergist from Pongamia pinnata Using High-Speed Counter-Current Chromatography Combined with Isobolographic Analysis
- Author
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Hao Yin, Yubai Wei, Rouwen Chen, Si Zhang, Lijuan Long, Hang Yin, Xinpeng Tian, and Weihong He
- Subjects
high-speed counter-current chromatography (HSCCC) ,Pongamia pinnata ,natural synergists ,isobolographic analysis ,subtracted residue ,chemical subtraction ,Organic chemistry ,QD241-441 - Abstract
For identifying the synergistic compounds from Pongamia pinnata, an approach based on high-speed counter-current chromatography (HSCCC) combined with isobolographic analysis was designed to detect the synergistic effects in the complex mixture [...]
- Published
- 2017
- Full Text
- View/download PDF
21. MULABS: Multi-Task Learning with Attention-Based Scoring for Click-Through Rate Prediction on Sparse Data in Healthcare Real-World Scenarios.
- Author
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Tu Tu, Jing Zhang, Yue Wang, Hui Jin, and Megan Weihong He
- Published
- 2022
- Full Text
- View/download PDF
22. Multilevel Clustering-Evolutionary Random Support Vector Machine Cluster Algorithm-Based Functional Magnetic Resonance Imaging in Diagnosing Cerebral Ischemic Stroke.
- Author
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Kun Fan, Ting Zhang, and Weihong He
- Published
- 2021
- Full Text
- View/download PDF
23. Multi-scale patches based image denoising using weighted nuclear norm minimisation.
- Author
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Yuli Fu 0001, Junwei Xu, Youjun Xiang, Zhen Chen 0010, Tao Zhu 0002, Lei Cai, and Weihong He
- Published
- 2020
- Full Text
- View/download PDF
24. Fluxes of riverine nutrient to the Zhujiang River Estuary and its potential eutrophication effect
- Author
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Li Zhang, Yumin Yang, Weihong He, Jie Xu, and Ruihuan Li
- Subjects
Aquatic Science ,Oceanography - Published
- 2022
25. Use of Passive Sampling in Edna Metabarcoding Technology: Monitoring of Fish Diversity in the Jiangmen Coastal Waters
- Author
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Li Zhang, Weiguo Zhou, Mengyu Jiao, Tian Xie, Mujiao Xie, Hanying Li, Anning Suo, Weizhong Yue, Weihong He, and Dewen Ding
- Published
- 2023
26. Experimental Investigation on Void and Compressive Response of Cnts Reinforced Filament-Wound Riser with Metal Liner
- Author
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Zan Liu, Jian Guo, Chao Kang, Jun Zhan, Bo Deng, Weihong He, Shuang Ye, Jinfeng Liu, Zhen Chen, and Yong Chen
- Subjects
History ,Environmental Engineering ,Polymers and Plastics ,Ocean Engineering ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2023
27. Regulatory Mechanism of MicroRNA-9 / Long Non-Coding RNA XIST Expression on Mouse Macrophage RAW264.7 Apoptosis Induced by Oxidized Low Density Lipoprotein
- Author
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Xiaoling, Zhang, Si, Wang, Yuyan, Cai, Weihong, He, Qing, Yang, and Chen, Li
- Subjects
proliferation ,fungi ,mir-9 ,apoptosis ,Bioengineering ,General Medicine ,Applied Microbiology and Biotechnology ,lncrna xist ,macrophages ,Lipoproteins, LDL ,Mice ,MicroRNAs ,Animals ,RNA, Long Noncoding ,TP248.13-248.65 ,Cell Proliferation ,Biotechnology - Abstract
It aims to analyze the influential mechanism of microRNA-9 (miR-9) and long non-coding RNA XIST (lncRNA XIST) expression on the proliferation and apoptosis of macrophages induced by oxidized-low density lipoprotein (ox-LDL). Firstly, lncRNA XIST overexpression vector was constructed, and then RAW264.7 cells were used as the research object. Methylthiazolyl tetrazolium (MTT) method, flow cytometry, and Western blot were used to detect and compare the differences of cell proliferation, apoptosis, and the expression levels of apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), matrix metalloproteinase-9 (MMP-9), and B-cell lymphoma-2 (Bcl-2) after ox-LDL induction and transfection of miR-9 mimic, miR-9 inhibitor and XIST expression vector, respectively. The results showed that lncRNA XIST overexpression vector was successfully constructed and transfected into cells, wh5ich can inhibit the expression level of miR-9. Compared with the normal control group, ox-LDL can inhibit cell proliferation, promote cell apoptosis, and increase the expression level of target protein. Moreover, transfection of XIST expression vector based on ox-LDL induction can significantly enhance the inhibition of cell proliferation, and promote cell apoptosis and the expression of target protein. Transfection of miR-9 mimic can improve the biological changes induced by ox-LDL. After co-transfection of miR-9 mimic and XIST expression vector based on ox-LDL induction, cell proliferation, apoptosis, and target protein expression level were not significantly different from those induced by ox-LDL alone. In summary, the increased expression level of miR-9 can inhibit the apoptosis of macrophages induced by ox-LDL. lncRNA XIST can positively regulate the apoptosis of macrophages induced by ox-LDL.
- Published
- 2022
28. Stagewise collapse of biotic communities and its relations to oxygen depletion along the north margin of Nanpanjiang Basin during the Permian–Triassic transition
- Author
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Han Wang, Weihong He, Yifan Xiao, Tinglu Yang, Kexin Zhang, Huiting Wu, Yafei Huang, Xingfang Peng, and Shunbao Wu
- Subjects
Paleontology ,Oceanography ,Ecology, Evolution, Behavior and Systematics ,Earth-Surface Processes - Published
- 2023
29. Integrated Radiolarian and Conodont Biostratigraphy of the Middle to Late Permian Linghao Formation in Northwestern Guangxi, South China
- Author
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Qinglai Feng, Taniel Danelian, Shan Chang, Maliha Zareen Khan, Lei Zhang, Weihong He, Qiangfen Ma, Ito Tsuyoshi, Jun Wu, Dong-xun Yuan, Marie Béatrice Forel, and Martial Caridroit
- Subjects
Paleontology ,South china ,biology ,Permian ,Geology ,Biostratigraphy ,biology.organism_classification ,Conodont ,Radiolaria - Published
- 2021
30. In Vitro Shoot Culture of Sesuvium portulacastrum: An Important Plant for Phytoremediation
- Author
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Weihong He, Dan Wang, Nan Yang, Dingding Cao, Xiaofeng Chen, Jianjun Chen, and Xiangying Wei
- Subjects
micropropagation ,Agriculture (General) ,axillary shoots ,food and beverages ,Plant Science ,disease-free plants ,in vitro rooting ,phytoremediation ,Sesuvium portulacastrum L ,shoot culture ,Agronomy and Crop Science ,Food Science ,S1-972 - Abstract
Sesuvium portulacastrum L., a member of the family Aizoaceae, is an important coastal halophyte. Due to its adaptability to salinity and heavy metals, S. portulacastrum has now been widely used for the phytoremediation of saline soils and wastewater and the protection of the coast from erosion. The increasing use of this plant requires a large number of propagules. Stem cutting propagation and seed germination cannot meet this demand, and such propagations can initiate and spread diseases. A recent occurrence of Bipolaris sesuvii J.Z. Zhang and Gibbago trianthemae E.G. Simmons in S. portulacastrum resulted in the substantial loss of the plants during the remediation of aquaculture wastewater. Thus, there is an urgent need for establishing efficient methods of propagating disease-free starting materials. In the present study, we evaluated different growth regulators in the induction of axillary shoots from nodal explants cultured on Murashige and Skoog medium and identified that zeatin (ZT) and α-naphthaleneacetic acid (NAA) was an appropriate combination for inducing high numbers of axillary shoots. The nodal explants were then cultured on MS medium supplemented with different concentrations of ZT and NAA, and the combination of ZT at 1.0 mg L−1 and NAA at 0.3 mg L−1 induced more than 12 axillary shoots per explant. The axillary shoots were excised to produce microcuttings or microshoots, which were rooted on half-strength MS medium supplemented with different concentrations of indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA). The results showed that IBA at 0.6 mg L−1 induced 91.7% of the microcuttings to root with root numbers of over 36 per cutting. The rooted plantlets were healthy and true-to-type and grew vigorously in plug trays or plastic containers with a 100% survey rate in a greenhouse. Thus, this established protocol could be used for the rapid propagation of genetically identical and disease-free plants of S. portulacastrum for phytoremediation and the protection of shoreline soils from erosion.
- Published
- 2022
31. Inhibition of myocardial cathepsin-L release during reperfusion following myocardial infarction improves cardiac function and reduces infarct size
- Author
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Elspeth B. Elliott, Aadil Shaukat, Weihong He, Kristopher Ford, Lisa McArthur, Katrin Nather, Mitchell Lindsay, Colin Berry, Stuart Hood, Ali Abdullah I. Zaeri, Catherine Hawksby, Tamara P. Martin, John D. McClure, Keith G. Oldroyd, David Carrick, Paul Rocchiccioli, Hany Eteiba, Keith M. Channon, Andrew Davie, Dylan O'Toole, Margaret McEntegart, Mark C. Petrie, Kenneth Mangion, Charlotte S. McCarroll, Stuart Watkins, Mathew M Y Lee, Stuart A. Nicklin, David Corcoran, Christopher M. Loughrey, Richard Good, Alexandra Riddell, Godfrey L. Smith, and Study, Oxford Acute Myocardial Infarction (OxAMI)
- Subjects
0301 basic medicine ,Cardiac function curve ,medicine.medical_specialty ,Physiology ,medicine.medical_treatment ,Myocardial Infarction ,chemistry.chemical_element ,Myocardial Reperfusion ,Myocardial Reperfusion Injury ,030204 cardiovascular system & hematology ,Calcium ,Cathepsin L ,03 medical and health sciences ,Percutaneous Coronary Intervention ,0302 clinical medicine ,Physiology (medical) ,Internal medicine ,medicine ,Humans ,Myocardial infarction ,biology ,business.industry ,Percutaneous coronary intervention ,medicine.disease ,Cathepsins ,Treatment Outcome ,030104 developmental biology ,chemistry ,Apoptosis ,Heart failure ,Reperfusion ,Cardiology ,biology.protein ,ST Elevation Myocardial Infarction ,Cardiology and Cardiovascular Medicine ,business ,Reperfusion injury - Abstract
Aims: \ud Identifying novel mediators of lethal myocardial reperfusion injury that can be targeted during primary percutaneous coronary intervention (PPCI) is key to limiting the progression of patients with ST-elevated myocardial infarction (STEMI) to heart failure. Here we show through parallel clinical and integrative preclinical studies the significance of the protease cathepsin-L on cardiac function during reperfusion injury.\ud \ud Methods and Results: \ud We found that direct cardiac release of cathepsin-L in STEMI patients (n = 76) immediately post-PPCI leads to elevated serum cathepsin-L levels and that serum levels of cathepsin-L in the first 24 hour post-reperfusion are associated with reduced cardiac contractile function and increased infarct size. Preclinical studies, demonstrate that inhibition of cathepsin-L release following reperfusion injury with CAA0225 reduces infarct size and improves cardiac contractile function by limiting abnormal cardiomyocyte calcium handling and apoptosis.\ud \ud Conclusion: \ud Our findings suggest that cathepsin-L is a novel therapeutic target that could be exploited clinically to counteract the deleterious effects of acute reperfusion injury after an acute STEMI.\ud \ud Translational perspective: \ud New therapeutic targets are urgently required to limit myocardial damage after reperfusion injury. We identified cardiac release of the protease cathepsin-L among patients following primary percutaneous coronary intervention (PPCI). Elevated serum levels of cathepsin-L were associated with reduced contractile function and increased infarct size at 24 hour and 6 months post-PPCI. Work conducted using animal models indicated that cardiac release of cathepsin-L mediated cardiac dysfunction following reperfusion injury. Specific inhibition of cathepsin-L prevented abnormal calcium handling, reduced infarct size and improved contractile function. These novel findings offer the prospect of targeting cathepsin-L-mediated cardiac dysfunction after PPCI.
- Published
- 2021
32. Origin and age of the Shenshan tectonic mélange in the Jiangshan-Shaoxing-Pingxiang Fault and late Early Paleozoic juxtaposition of the Yangtze Block and the West Cathaysia terrane, South China
- Author
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Leiming Yin, Lijun Wang, Weihong He, Kexin Zhang, and Shoufa Lin
- Subjects
Paleontology ,geography ,South china ,geography.geographical_feature_category ,Paleozoic ,Block (telecommunications) ,Geology ,Mélange ,Fault (geology) ,Terrane - Abstract
When and how the Yangtze Block (Yangtze) and the West Cathaysia terrane (West Cathaysia) in South China were amalgamated are critical to a better understanding of the Neoproterozoic to early Paleozoic tectonic evolution of South China and remain highly debatable. A key to this debate is the tectonic significance of the Jiangshan-Shaoxing-Pingxiang (JSP) Fault, the boundary between Yangtze and West Cathaysia. The Shenshan mélange along the JSP Fault has the typical block-in-matrix structure and is composed of numerous shear zone-bounded slivers/lenses of rocks of different types and ages that formed in different tectonic environments, including middle to late Tonian volcanic and volcanogenic sedimentary rocks (turbidite) of arc/back-arc affinity, a series of middle Tonian ultramafic to mafic plutonic rocks of oceanic island basalt affinity, a carbonaceous shale that was deposited in a deep marine environment, and a red mudstone. U-Pb zircon ages and acritarch assemblages (Leiosphaeridia-Brocholaminaria association) found in the turbidite confirm its Tonian age, and fossils from the carbonaceous shale (Asteridium-Comasphaeridium and Skiagia-Celtiberium-Leiofusa) constrains its age to the Early to Middle Cambrian. Field relationships and available age data leave no doubt that the ultramafic-mafic rocks are exotic blocks (rather than intrusions) in the younger metasedimentary rocks. We conclude that the Shenshan mélange is not an ophiolitic mélange, but rather a tectonic mélange that formed as a result of movement along the JSP Fault in the early Paleozoic. We suggest that Yangtze and West Cathaysia were two separate microcontinents, were accreted to two different parts of the northern margin of Gondwana in the early Early Paleozoic, and juxtaposed in the late Early Paleozoic through strike-slip movement along the JSP Fault. We further suggest that the ca. 820 Ma collision in the Jiangnan Orogen took place between Yangtze and a (micro)continent that is now partly preserved as the Huaiyu terrane and was not related to West Cathaysia. We compare our model for South China with the accretion of terranes in the North American Cordillera and propose a similar model for the relationship between the Avalon and Meguma terranes in the Canadian Appalachians, i.e., the two terranes were accreted to two different parts of the Laurentian margin and were later juxtaposed through margin-parallel strike slip faulting.
- Published
- 2021
33. An Adaptive Batched Patch Caching Scheme for Multimedia Streaming.
- Author
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ShaoHua Qin, Weihong He, Zimu Li, and Jianping Hu
- Published
- 2004
- Full Text
- View/download PDF
34. Multi‐scale patches based image denoising using weighted nuclear norm minimisation
- Author
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Weihong He, Zhen Chen, Yuli Fu, Lei Cai, Junwei Xu, Youjun Xiang, and Tao Zhu
- Subjects
business.industry ,Computer science ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Matrix norm ,020206 networking & telecommunications ,Pattern recognition ,02 engineering and technology ,Minimisation (clinical trials) ,Image texture ,Computer Science::Computer Vision and Pattern Recognition ,Signal Processing ,0202 electrical engineering, electronic engineering, information engineering ,020201 artificial intelligence & image processing ,Computer Vision and Pattern Recognition ,Artificial intelligence ,Electrical and Electronic Engineering ,Image denoising ,business ,Software ,Image restoration - Abstract
As a prior knowledge, non-local self-similarity (NSS) has been widely utilised in ill-posed problems. Actually, similar textures appear not only in a single scale, but also in different scales. Unlike most existing patch-based methods that only explore NSS in the same scale, a multi-scale patches based image denoising algorithm is proposed in this study. The authors have designed a multi-scale strategy to expand the search space of block-matching, which will increase the probability of finding more similar patches. After that, the weighted nuclear norm minimisation (WNNM) algorithm is employed to reveal latent clean patches. With the join of the multi-scale framework, the performance of WNNM can be improved. The proposed algorithm can be used to solve NSS-based image restoration tasks. In this study, mainly image denoising is studied, and its effectiveness is derived through experiments on widely used test images.
- Published
- 2020
35. Verifiability of genus-level classification under quantification and parsimony theories: a case study of follicucullid radiolarians
- Author
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Michael J. Benton, Noritoshi Suzuki, Weihong He, Chenyang Cai, Tinglu Yang, and Yifan Xiao
- Subjects
010506 paleontology ,0303 health sciences ,Multivariate statistics ,Ecology ,Phylogenetic tree ,Paleontology ,Biology ,Linear discriminant analysis ,01 natural sciences ,Maximum parsimony ,03 medical and health sciences ,Discriminant ,Genus ,Evolutionary biology ,Taxonomy (biology) ,General Agricultural and Biological Sciences ,Categorical variable ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,0105 earth and related environmental sciences - Abstract
The classical taxonomy of fossil invertebrates is based on subjective judgments of morphology, which can cause confusion, because there are no codified standards for the classification of genera. Here, we explore the validity of the genus taxonomy of 75 species and morphospecies of the Follicucullidae, a late Paleozoic family of radiolarians, using a new method, Hayashi's quantification theory II (HQT-II), a general multivariate statistical method for categorical datasets relevant to discriminant analysis. We identify a scheme of 10 genera rather than the currently accepted 3 genera (Follicucullus,Ishigaconus, andParafollicucullus). As HQT-II cannot incorporate stratigraphic data, a phylogenetic tree of Follicucullidae was reconstructed for 38 species using maximum parsimony. Six lineages emerged, roughly in concordance with the results of HQT-II. Combined with parsimony ancestral state reconstruction, the ancestral group of this family isHaplodiacanthus. Five other groups were discriminated, theParafollicucullus,Curvalbaillella,Pseudoalbaillella,Longtanella, andFollicucullus–Cariverlineages. The morphological evolution of these lineages comprises a minimum essential list of eight states of four traits. HQT-II is a novel discriminant analytical multivariate method that may be of value in other taxonomic problems of paleobiology.
- Published
- 2020
36. Observation system simulation experiments using an ensemble-based method in the northeastern South China Sea
- Author
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Gengxin Chen, Xiaoli Zou, Qiang Xie, Danian Liu, Ruiwen Wang, Weihong He, Chuanyu Liu, Feng Cheng, Zhaozheng Wang, Dong Ye, Wu Geng, and Yeqiang Shu
- Subjects
Observation system ,South china ,Climatology ,Vertical direction ,Sea-surface height ,Oceanography ,Cyclonic gyre ,Geology ,Water Science and Technology - Abstract
An ensemble-based method for the observation system simulation experiment (OSSE) is employed to design optimal observation stations and assess the present observation stations in the northeastern South China Sea (SCS). We employed the 20-year (1992–2012) sea surface height (SSH) data to design an array to monitor the intraseasonal to interannual variability. The results show that the most key region was found located at the northwest of Luzon Island (LI) where the energetic Luzon cyclonic gyre (LCG) occurs; other key regions include the edge of the LCG, the northwest of the Luzon Strait (LS), and the southwest of Taiwan, China. By contrast, we found that the present observation stations might oversample at the northwest of the LS and undersample at the northwest of LI. In addition, the optimal stations perform better in a larger area than the present stations. In vertical direction, the key layer is located within the upper 200-m depth, of which the surface and subsurface layers are most valuable to the observing system.
- Published
- 2020
37. The palaeoenvironmental and palaeobiogeographical significance of the Late Permian deep-water brachiopod fauna from Dongpan, South China, including descriptions ofMicromartiniaHe & Weldon gen. nov. (Micromartiniidae He & Weldon fam. nov.) andMinutomarginiferanom. nov
- Author
-
Weihong He, Yongbiao Wang, Han Wang, Tinglu Yang, Yifan Xiao, Elizabeth A. Weldon, Kexin Zhang, Shunbao Wu, and Huiting Wu
- Subjects
0106 biological sciences ,Systematics ,010506 paleontology ,South china ,Permian ,Fauna ,Paleontology ,Biostratigraphy ,010603 evolutionary biology ,01 natural sciences ,Archaeology ,Deep water ,Geography ,Genus ,Paleoecology ,0105 earth and related environmental sciences - Abstract
A new genus and species, Micromartinia kwangsiella gen. et sp. nov., is erected based on material from the Talung Formation (upper Changhsingian), Guangxi, South China. Based on a phylogenetic anal...
- Published
- 2019
38. A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper
- Author
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Qianqian Meng, Weihong He, Tingting Zhang, Deyi Guan, Shaoliang Mou, Feng Gao, and Shuilin He
- Subjects
Capsicum annuum ,China ,Nicotiana benthamiana ,Plant Science ,Bimolecular fluorescence complementation ,Gene Expression Regulation, Plant ,Pepper ,Electrophoretic mobility shift assay ,Cysteine ,Immune response ,Protein kinase A ,Disease Resistance ,Ralstonia solanacearum ,CaCRK5 ,biology ,Kinase ,fungi ,Botany ,food and beverages ,biology.organism_classification ,Cell biology ,QK1-989 ,Capsicum ,Chromatin immunoprecipitation ,Protein Kinases ,Research Article - Abstract
Background Cysteine-rich receptor-like kinases (CRKs) represent a large subfamily of receptor-like kinases and play vital roles in diverse physiological processes in regulating plant growth and development. Results CaCRK5 transcripts were induced in pepper upon the infection of Ralstonia solanacearum and treatment with salicylic acid. The fusions between CaCRK5 and green fluorescence protein were targeted to the plasma membrane. Suppression of CaCRK5 via virus-induced gene silencing (VIGS) made pepper plants significantly susceptible to R. solanacearum infection, which was accompanied with decreased expression of defense related genes CaPR1, CaSAR8.2, CaDEF1 and CaACO1. Overexpression of CaCRK5 increased resistance against R. solanacearum in Nicotiana benthamiana. Furthermore, electrophoretic mobility shift assay and chromatin immunoprecipitation coupled with quantitative real-time PCR analysis revealed that a homeodomain zipper I protein CaHDZ27 can active the expression of CaCRK5 through directly binding to its promoter. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) analyses suggested that CaCRK5 heterodimerized with the homologous member CaCRK6 on the plasma membrane. Conclusions Our data revealed that CaCRK5 played a positive role in regulating immune responses against R. solanacearum infection in pepper.
- Published
- 2021
39. Association between serum NLRP3 and malignant brain edema in patients with acute ischemic stroke
- Author
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Shihong Zhang, Weihong He, Ming Liu, Yanan Wang, Simiao Wu, and Hexiao Huang
- Subjects
Male ,medicine.medical_specialty ,Neurology ,Acute ischemic stroke ,Brain Edema ,Logistic regression ,Gastroenterology ,Severity of Illness Index ,Brain Ischemia ,Basal (phylogenetics) ,Midline shift ,NLRP3 ,Internal medicine ,NLR Family, Pyrin Domain-Containing 3 Protein ,Medicine ,Humans ,Neurochemistry ,RC346-429 ,Stroke ,Malignant brain edema ,Ischemic Stroke ,business.industry ,Research ,Brain ,General Medicine ,Hypoxia (medical) ,medicine.disease ,Neurology (clinical) ,Neurosurgery ,Neurology. Diseases of the nervous system ,medicine.symptom ,business ,Biomarkers - Abstract
Background We aimed to explore the association of serum level of the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) and its related inflammatory biomarkers (hypoxia inducible factor-1α, cathepsin B, caspase-1 and matrix metalloproteinase-9) with malignant brain edema (MBE) in patients with acute ischemic stroke. Methods We prospectively enrolled patients with acute ischemic stroke admitted Results 200 patients (69.3 ± 14.3 years; male 55 %) were included for analysis, of whom 26 patients developed MBE (median time from stroke onset to MBE 32.5 h). Compared with patients without MBE, those with MBE had higher level of serum concentration of NLRP3 (median time from onset to blood collection 3 h, 1.85 ng/ml vs. 1.11 ng/ml, P = 0.026). NLRP3 level was positively correlated with NIHSS on admission (Spearman ρ = 0.18, P = 0.01) and the association between NLRP3 and MBE was attenuated (OR 1.47, 95 % CI 0.88–2.46, P = 0.138) after adjusting for age and NIHSS. There was no significant difference in other biomarkers between MBE and non-MBE groups. Conclusions There was a trend of association between a higher level of serum concentration of NLRP3 and an increased risk of MBE after ischemic stroke, possibly confounded by the severity of stroke, which is worth further validation in large cohort studies.
- Published
- 2021
40. Size variation of brachiopods from the Late Permian through the Middle Triassic in South China: Evidence for the Lilliput Effect following the Permian-Triassic extinction
- Author
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Fengyu Wang, Jing Chen, Shunbao Wu, Jinnan Tong, Weihong He, and Haijun Song
- Subjects
Extinction event ,010506 paleontology ,South china ,Extinction ,Permian ,Early Triassic ,Paleontology ,social sciences ,010502 geochemistry & geophysics ,Oceanography ,01 natural sciences ,humanities ,Evolution of brachiopods ,Ecology, Evolution, Behavior and Systematics ,Permian–Triassic extinction event ,Geology ,0105 earth and related environmental sciences ,Earth-Surface Processes ,Invertebrate - Abstract
Whether body size reduction (the Lilliput Effect) occurred in the Early Triassic invertebrates remains a matter of debate. Here, we investigate the size evolution of brachiopods spanning the Late Permian through the Early to Middle Triassic based on 3316 brachiopod specimens from South China. Our results show that the maximum and median size among species decreased dramatically from the latest Permian (Changhsingian) to the earliest Triassic (Griesbachian), and then increased during the Olenekian and Anisian. Our data support the Lilliput Effect on brachiopods during the Permian-Triassic mass extinction and its immediate aftermath. Size plots at species, genus, and family levels suggest that the earliest Triassic size reduction resulted from two factors: the preferential extinction of large brachiopod taxa (size-selective extinction), and the size reductions in the survivors. Persistent increases in brachiopod size in the Olenekian and Anisian are likely the result of the extinction of small survivors, the appearance of large-sized species and the Cope's rule that the new lineages tend to increase in body size over the early evolutionary time. The cause of the earliest Triassic size reduction of brachiopods might be environmental pressures such as global warming, oceanic anoxia, and increased water turbidity.
- Published
- 2019
41. Impacts of conventional and biodegradable microplastics on juvenile Lates calcarifer: Bioaccumulation, antioxidant response, microbiome, and proteome alteration
- Author
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Mujiao, Xie, Peng, Xu, Weiguo, Zhou, Xiangrong, Xu, Hengxiang, Li, Weihong, He, Weizhong, Yue, Li, Zhang, Dewen, Ding, and Anning, Suo
- Subjects
Proteome ,Polyethylene ,Microbiota ,Microplastics ,Animals ,Aquatic Science ,Oceanography ,Bioaccumulation ,Plastics ,Pollution ,Antioxidants ,Water Pollutants, Chemical ,Perciformes - Abstract
Discarded plastic bag is a main component of marine debris, posing potential threats to marine biota. This study was conducted to assess the potential effects of microplastics on juvenile Lates calcarifer. Fish were exposed via diet to two microplastic types from conventional polyethylene (PE) and biodegradable (Bio) plastic bags for 21 days. Antioxidative enzymes activity, intestinal microbiome and proteome were determined. PE and Bio microplastics were found to accumulate in gastrointestinal tracts, and no mortality was observed. Microplastics exposure did not induce significant antioxidant response except for the glutathione reductase (GR) modulation. Intestinal microbiome diversity decreased significantly in PE group based on Simpson index. Both types of microplastics induced proteome modulation by down-regulating proteins associated with immune homeostasis. Bio microplastics maintained higher intestinal microbial diversity and induced more proteins alteration than PE microplastics. This study provides toxicological insights into the impacts of conventional and biodegradable microplastics on juvenile L. calcarifer.
- Published
- 2022
42. Review for 'The implications of the giant bivalve family Alatoconchidae for the end‐Guadalupian (Middle Permian) extinction event'
- Author
-
Weihong He
- Subjects
Paleontology ,Event (relativity) ,Geology ,Permian–Triassic extinction event - Published
- 2021
43. Review for 'Frasnian–Tournaisian (Late Devonian to Earliest Carboniferous) lithostratigraphy and biostratigraphy of Hainan Island, South China'
- Author
-
Weihong He
- Subjects
Tournaisian ,Paleontology ,South china ,Carboniferous ,Lithostratigraphy ,Late Devonian extinction ,Biostratigraphy ,Geology - Published
- 2020
44. Two pulses of increasing terrestrial input to marine environment during the Permian–Triassic transition
- Author
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Wei Liao, Guoshan Li, Hao Yang, Yongbiao Wang, Weihong He, Zhixing Yi, and Yafei Huang
- Subjects
Extinction event ,Extinction ,Permian ,Carbonate platform ,Paleontology ,Flux ,Oceanography ,Arctic ,Marine ecosystem ,Terrestrial ecosystem ,Ecology, Evolution, Behavior and Systematics ,Geology ,Earth-Surface Processes - Abstract
The destruction of both terrestrial and marine ecosystems generally shows a process of rapid extinction followed by the wipe out of the Permian-type relics in the Permian–Triassic mass extinction, but the relationship between the terrestrial and marine ecosystem collapses is not clear until now. The terrestrial input from the land to marine system is an important link between them. Previous study has demonstrated a substantial increase of terrestrial influx to the marine water most likely corresponding to the Permian–Triassic mass extinction. Our new geochemical data from the Laolongdong section in the shallow marine carbonate platform of South China, reveal that the elevated terrestrial input nearby the Permian–Triassic Boundary (PTB) is actually marked by a two-pulse scenario. The first pulse represents a ~ 3- to ~ 6-fold increase of the terrestrial flux. The onset of the increase took place in the extinction horizon at the basal part of microbialites at Laolongdong, basically corresponding to Bed 24a of Meishan section and prior to the end-Permian mass extinction zenith; then the increase continued and spanned the extinction zenith. The second pulse is characterized a ~ 21- to ~ 35-fold increase in the terrestrial flux, equivalent to the widespread, abundant presence of mudstones in the earliest Triassic and roughly corresponding to the stage where the Permian-type survivors were thoroughly wiped out. A two-pulse terrestrial input is also clearly recorded at Cili, Shangsi and Meishan of South China, western Australia and Arctic Canada. Two episodes of the enhanced terrestrial input provide strong evidence for the devastation of the terrestrial ecosystem and insights into the reasonable explanation for the relationship between terrestrial and marine ecosystem collapses nearby the PTB.
- Published
- 2022
45. Tonian Tectonic‐strata Regions and Geological Significance in China
- Author
-
Kexin Zhang, Fengli Yang, Bowen Song, Yang Yu, Weihong He, Xiaohu Kou, Mansheng Luo, Lijun Wang, Zhongjie Ma, and Yadong Xu
- Subjects
Tectonics ,Craton ,geography ,geography.geographical_feature_category ,Tonian ,Geochemistry ,Geology ,Sedimentary rock ,China ,Palaeogeography - Published
- 2020
46. An early Paleozoic tectonic mélange at the western margin of West Cathaysia: constraints from organic‐walled microfossils
- Author
-
Lijun Wang, Weihong He, Shoufa Lin, Kexin Zhang, and Leiming Yin
- Subjects
Paleontology ,Paleozoic ,Margin (machine learning) ,Acritarch ,Geology ,Mélange - Published
- 2020
47. Stratigraphic Framework of the Cryogenian in China
- Author
-
Yang Yu, Qin Ye, Bowen Song, Yang Fengli, Weihong He, Wang Jiaxuan, Xu Yadong, Xiaohu Kou, and Kexin Zhang
- Subjects
Paleontology ,Geology ,Structural basin ,China ,Palaeogeography - Published
- 2020
48. Image compressed sensing recovery via adaptive dictionary learning
- Author
-
Youjun Xiang, Lei Cai, Tao Zhu, Junwei Xu, Weihong He, and Yuli Fu
- Subjects
Computer science ,business.industry ,media_common.quotation_subject ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Image (mathematics) ,Monotone polygon ,Compressed sensing ,Computer vision ,Extraction methods ,Quality (business) ,Artificial intelligence ,business ,Dictionary learning ,media_common - Abstract
This paper addresses the image compressed sensing recovery problem. To improve the recovery quality, instead of using a fixed dictionary that is generally a universal one trained in an off-line manner for sparse representations of image patches, we adopt an adaptive dictionary learning strategy. Inspired by the monotone fast iterative shrinkagethresholding algorithm, a dictionary learning algorithm is introduced in this work. Also, we abandon the classic method that breaks an image into fully overlapping patches, and propose a new overlapping patches extraction method, which decreases the number of patches and saves much run-time, while achieves similar recovery qualities.
- Published
- 2020
49. An E-commerce recommender system based on content-based filtering
- Author
-
Weihong, He and Yi, Cao
- Published
- 2006
- Full Text
- View/download PDF
50. The Changhsingian Foraminiferal Fauna of the Meishan D Section, Zhejiang, China, and their Ecostratigraphic Implications
- Author
-
Zongyan Zhang, Weihong He, Xiaohu Kou, Yi Wei, Liang Luo, and Xue Ke
- Subjects
Foraminifera ,Paleontology ,010504 meteorology & atmospheric sciences ,biology ,Section (archaeology) ,Fauna ,Geology ,010502 geochemistry & geophysics ,biology.organism_classification ,China ,01 natural sciences ,0105 earth and related environmental sciences - Published
- 2018
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