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In the last years coating of surfaces in the presence of dopamine or other catecholamines in oxidative conditions to yield "polydopamine" films has become a popular, easy and versatile coating methodology. Polydopamine(s) offer(s) also a rich chemistry allowing to post-functionalize the obtained coatings with metal nanoparticles with polymers and proteins. However, the interactions either of covalent or non-covalent nature between polydopamine and biomolecules has only been explored more recently. They allow polydopamine to become a material, in the form of nanoparticles, membranes and other assemblies, in its own right not just as a coating. It is the aim of this review to describe the most recent advances in the design of composites between polydopamine and related eumelanin like materials with biomolecules like proteins, nucleotides, oligosaccharides and lipid assemblies. Furthermore, the interactions between polydopamine and living cells will be also reported.

© 2017 American Chemical Society.The design and use of materials in the nanoscale size range for addressing medical and health-related issues continues to receive increasing interest. Research in nanomedicine spans a multitude of areas, including drug delivery, vaccine development, antibacterial, diagnosis and imaging tools, wearable devices, implants, high-throughput screening platforms, etc. using biological, nonbiological, biomimetic, or hybrid materials. Many of these developments are starting to be translated into viable clinical products. Here, we provide an overview of recent developments in nanomedicine and highlight the current challenges and upcoming opportunities for the field and translation to the clinic.

Live cell imaging is an important technique applied to a number of Drosophila tissues used as models to investigate topics such as axis specification, cell differentiation and organogenesis (1). Correct preparation of the experimental samples is a crucial, often neglected, step. The goal of preparation is to ensure physiological relevance and to establish optimal imaging conditions. To maintain tissue viability, it is critical to avoid dehydration, hypoxia, overheating or medium deterioration (2). The Drosophila egg chamber is a well established system for examining questions relating, but not limited, to body patterning, mRNA localization and cytoskeletal organization (3,4). For early- and mid-stage egg chambers, mounting in halocarbon oil is good for survival in that it allows free diffusion of oxygen, prevents dehydration and hypoxia and has superb optical properties for microscopy. Imaging of fluorescent proteins is possible through the introduction of transgenes into the egg chamber or physical injection of labeled RNA, protein or antibodies (5-7). For example, addition of MS2 constructs to the genome of animals enables real time observation of mRNAs in the oocyte (8). These constructs allow for in vivo labeling of mRNA through utilization of the MS2 bacteriophage RNA stem loop interaction with its coat protein (9). Here, we present a protocol for the extraction of ovaries as well as isolating individual ovarioles and egg chambers from the female Drosophila. For a detailed description of Drosophila oogenesis see Allan C. Spradling (1993, reprinted 2009) (10).

Medication errors including inappropriate prescriptions and drug omissions are one of the causes of adverse drug events in children. Our aim was to develop a preliminary screening tool to detect omissions and inappropriate prescriptions in pediatrics based on French and international guidelines.Disease classification was based on the prevalence rate of pathology and hospital statistics. The criteria were obtained by reviewing many French and international references. The Delphi consensus technique was used to establish the content validity of POPI. The level of agreement and the proposals of healthcare professionals was noted on a nine-point Likert scale.The criteria were categorized according to the main physiological systems (gastroenterology, respiratory infections, pain, neurology, dermatology, and miscellaneous). They were distributed to 16 French pediatric panelists (eight pharmacists, eight pediatricians who were hospital-based [50%] or working in the community [50%]). After two rounds of the Delphi process, 101 of 108 criteria were chosen with strong consensus (76 inappropriate prescriptions and 25 omissions).POPI is the first screening tool to detect inappropriate prescriptions and omissions in pediatrics. It is now necessary to conduct a prospective study to determine inter-rater reliability and the tool's detection capacity.

Since our public officials now lack the courage and political will to raise taxes or to constrain Medicare and Medicaid benefits, we can expect that: 1) the private sector and future generations will pay an increasing share of these entitlements, 2) major cutbacks in Medicare, Medicaid, and health maintenance organization (HMO) reimbursement will result in the aged and poor experiencing decreased access to care and will hasten the current thrust of physicians, hospitals, and insurers forming powerful health networks, 3) these new regional alliances functioning as virtual monopolies will result in the public demanding that state-sponsored health services commissions be established, and 4) the weakest health networks, often in underserved areas, will be fiscally squeezed by inadequate reimbursement, so that by the turn of the century, many of these facilities and HMOs will seek bankruptcy protection as a means of restructuring their long-term debt.

The nation's health maintenance organizations, preferred-provider organizations, independent practice associations, and similar managed-care efforts are not well positioned to take a leadership role in a nationwide universal access or national health insurance plan. They--with the possible exception of some large staff and group health maintenance organizations--have been unable to show uniformly that they can contain costs, provide better access or higher quality of care, and achieve greater patient satisfaction than fee-for-service endeavors. As the United States pursues universal access as a step toward national health insurance, the managed-care plans will continue to increase their numbers of subscribers. They will not, however, be able to enroll large numbers of the young, low-income employees and their dependents who account for most of the 63 million people uninsured sometime during each year. Under national health insurance, there might be an option for some health maintenance organizations to negotiate capitated payments. The vast majority of the nation's physicians, however, will reluctantly embrace a centrally managed fee-for-service approach rather than a salary or capitated reimbursement method, leaving only a trace of the competitive managed-care plan theme in a future, primarily monolithic, national health care system.