144 results on '"Weiliang Wu"'
Search Results
2. Assessment of osteopontin as an early nephrotoxicity indicator in human renal proximal tubule cells and its application in evaluating lanthanum-induced nephrotoxicity
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Yingsi Chen, Feifei Xu, Xiaoxuan Xiao, Huiqin Chi, Yuefei Lai, Xiuqin Lin, Qiuyun Li, Jia Song, Weiliang Wu, Ziyin Li, and Xingfen Yang
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IPSCs ,HK-2 ,Nephrotoxicity ,In vitro cell model ,Osteopontin ,Lanthanum ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
Nephrotoxicity is a common adverse effect induced by various chemicals, necessitating the development of reliable toxicity screening models for nephrotoxicity assessment. In this study, we assessed a group of nephrotoxicity indicators derived from different toxicity pathways, including conventional endpoints and kidney tubular injury biomarkers such as clusterin (CLU), kidney injury molecule-I (KIM-1), osteopontin (OPN), and neutrophil gelatinase-associated lipocalin (NGAL), using HK-2 and induced pluripotent stem cells (iPSCs)-derived renal proximal tubular epithelial-like cells (PTLs). Among the biomarkers tested, OPN emerged as the most discerning and precise marker. The predictive potential of OPN was tested using a panel of 10 nephrotoxic and 5 non-nephrotoxic compounds. The results demonstrated that combining OPN with the half-maximal inhibitory concentration (IC50) enhanced the diagnostic accuracy in both cellular models. Additionally, PTLs cells showed superior predictive efficacy for nephrotoxicity compared to HK-2 cells in this investigation. The two cellular models were utilized to evaluate the nephrotoxicity of lanthanum. The findings indicated that lanthanum possesses nephrotoxic properties; however, the degree of nephrotoxicity was relatively low, consistent with the outcomes of in vivo experiments.
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- 2024
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3. The Bioaccessibility and Bioavailability of Pentachlorophenol in Five Animal-Derived Foods Measured by Simulated Gastrointestinal Digestion
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Quan Zhou, Huiming Chen, Liangliang Li, Yongning Wu, Xingfen Yang, Aimin Jiang, and Weiliang Wu
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pentachlorophenol ,in vitro digestive model ,bioaccessibility ,bioavailability ,culinary treatment ,food matrix ,Chemical technology ,TP1-1185 - Abstract
Pentachlorophenol (PCP) is a ubiquitous emerging persistent organic pollutant detected in the environment and foodstuffs. Despite the dietary intake of PCP being performed using surveillance data, the assessment does not consider the bioaccessibility and bioavailability of PCP. Pork, beef, pork liver, chicken and freshwater fish Ctenopharyngodon Idella-fortified by three levels of PCP were processed by RIVM and the Caco-2 cell model after steaming, boiling and pan-frying, and PCP in foods and digestive juices were detected using isotope dilution–UPLC-MS/MS. The culinary treatment and food matrix were significantly influenced (p < 0.05) in terms of the bioaccessibility and bioavailability of PCP. Pan-frying was a significant factor (p < 0.05) influencing the digestion and absorption of PCP in foods, with the following bioaccessibility: pork (81.37–90.36%), beef (72.09–83.63%), pork liver (69.11–78.07%), chicken (63.43–75.52%) and freshwater fish (60.27–72.14%). The bioavailability was as follows: pork (49.39–63.41%), beef (40.32–53.43%), pork liver (33.63–47.11%), chicken (30.63–40.83%) and freshwater fish (17.14–27.09%). Pork and beef with higher fat content were a key factor in facilitating the notable PCP bioaccessibility and bioavailability (p < 0.05). Further, the exposure of PCP to the population was significantly reduced by 42.70–98.46% after the consideration of bioaccessibility and bioavailability, with no potential health risk. It can improve the accuracy of risk assessment for PCP.
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- 2024
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4. Effects of different drop height training on lower limb explosive and change of direction performance in collegiate Sanda athletes
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Gen Li, Weiliang Wu, Kai Zhen, Shiyan Zhang, Zhizhou Chen, Yuanyuan Lv, Xiao Hou, and Laikang Yu
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Sports medicine ,Physics ,Engineering ,Science - Abstract
Summary: The purpose of the present study was to examine the effects of 6 weeks of 40-, 60-, or 80-cm drop jump (DJ) training on lower limb explosive and change of direction (CoD) performance in collegiate Sanda athletes. Repeated-measure ANOVA revealed that there was a significant group × time interaction for standing long jump test (p = 0.006), counter movement jump test (p = 0.026), Illinois agility test (p = 0.003), square test (p = 0.018), Nebraska test (p = 0.027), t test (p = 0.032), and hexagon test (p = 0.012) due to the best performance observed at post-test compared with pre-test for DJ60 (effect size = 0.89–2.89), and the improvement was higher than that of the other groups. These findings suggest that 6 weeks of DJ training could improve the lower limb explosive and CoD performance in collegiate Sanda athletes and that 60 cm may be the optimal drop height.
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- 2023
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5. Hyperplastic Human Macromass Cartilage for Joint Regeneration
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Ya Wen, Yishan Chen, Weiliang Wu, Hong Zhang, Zhi Peng, Xudong Yao, Xianzhu Zhang, Wei Jiang, Youguo Liao, Yuan Xie, Xilin Shen, Heng Sun, Jiajie Hu, Hua Liu, Xiao Chen, Jiansong Chen, and Hongwei Ouyang
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cell plasticity ,customized expansion ,joint regeneration ,macromass cartilage ,Science - Abstract
Abstract Cartilage damage affects millions of people worldwide. Tissue engineering strategies hold the promise to provide off‐the‐shelf cartilage analogs for tissue transplantation in cartilage repair. However, current strategies hardly generate sufficient grafts, as tissues cannot maintain size growth and cartilaginous phenotypes simultaneously. Herein, a step‐wise strategy is developed for fabricating expandable human macromass cartilage (macro‐cartilage) in a 3D condition by employing human polydactyly chondrocytes and a screen‐defined serum‐free customized culture (CC). CC‐induced chondrocytes demonstrate improved cell plasticity, expressing chondrogenic biomarkers after a 14.59‐times expansion. Crucially, CC‐chondrocytes form large‐size cartilage tissues with average diameters of 3.25 ± 0.05 mm, exhibiting abundant homogenous matrix and intact structure without a necrotic core. Compared with typical culture, the cell yield in CC increases 2.57 times, and the expression of cartilage marker collagen type II increases 4.70 times. Transcriptomics reveal that this step‐wise culture drives a proliferation‐to‐differentiation process through an intermediate plastic stage, and CC‐chondrocytes undergo a chondral lineage‐specific differentiation with an activated metabolism. Animal studies show that CC macro‐cartilage maintains a hyaline‐like cartilage phenotype in vivo and significantly promotes the healing of large cartilage defects. Overall, an efficient expansion of human macro‐cartilage with superior regenerative plasticity is achieved, providing a promising strategy for joint regeneration.
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- 2023
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6. A strontium/vancomycin composite coating on titanium implants for preventing bacterial infection and improving osseointegration
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Yiguo Shen, Kun Wang, Weiliang Wu, Wei Feng, Jiansong Chen, and Qiang Gao
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Titanium ,Strontium ,Vancomycin ,Osseointegration ,Anti-infection ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Postoperative bacterial infection and poor osseointegration with surrounding bone tissue are not only the critical causes of implant failure but also the major challenges in clinical application. Hereby, Titania (TiO2) microporous/nanofiber coating was firstly prepared on the surface of titanium (Ti) material by alkali-heat treatment process, and strontium (Sr)-doped coating (TS) was then prepared by hydrothermal synthesis. Finally, polydopamine-assisted co-deposition technology was utilized to deposit vancomycin (Van) onto TS surface (TSV), thus obtaining a medical composite coating with antibacterial property and improved osseointegration ability. According to antibacterial test, TSV has not only a 99.99% killing effect on methicillin-resistant Staphylococcus aureus (MRSA), but also a good anti-biofilm ability. The cell experiments showed that TSV could enhance the adhesion and proliferation of osteoblasts. Compared with pure Ti and TS, TSV induces the formation of bone apatite in simulated body fluid (SBF) test quickly, indicating that the coating has good biological activity. According to histopathological observation and bone morphology analysis, TSV showed highly effective infection prevention and excellent osteointegration in vivo. These test results suggested that it is feasible to prevent implant-associated infection and promote osseointegration by functionalization of the implant, so as to provide a fresh direction for clinical research.
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- 2023
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7. Bioaccessibility of Cadmium in Common Vegetables in Typical Environmental High Cadmium Region and the Health Risk Assessment
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Feifei XU, Yueqi LI, Jun LIN, Yuefei LAI, Ziyin LI, Xiuqin LIN, Yingsi CHEN, Weiliang WU, and Xingfen YANG
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cadmium ,vegetables ,bioaccessibility ,dietary exposure ,risk assessment ,Food processing and manufacture ,TP368-456 - Abstract
In order to assess the health risk of consumption of local planted vegetables in high-level Cd contaminated region, inductively coupled plasma mass spectrometry (ICP-MS) was employed to determine the elements of Cd, Mg, K, Ca, Cu and Zn in the commonly consumed vegetables collected from this region. The effects of the cooking methods, the process of gastrointestinal digestion, and the concentrations of elements on the bioaccessibility of Cd were investigated using in vitro RIVM model. In addition, the health risk of Cd exposure via vegetable consumption for the local residents were evaluated using the target hazard quotients (THQs). The results showed that the average contents of Cd in vegetables were ranged from 0.02 to 0.30 mg/kg. Cooking methods did influence the concentration of Cd in the samples, indicating that boiling significantly reduced the concentration of Cd in vegetables (P0.05). In contrast, boiling significantly increased the bioaccessibility of Cd (P0.05). The results of correlation analysis showed that Cd bioaccessibility had significantly negative correlation with K and Cu (P
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- 2022
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8. An emerging role of inflammasomes in spinal cord injury and spinal cord tumor
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Jiansong Chen, Yiguo Shen, Xiaobo Shao, and Weiliang Wu
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spinal cord injury ,spinal cord tumor ,inflammasome ,caspase-1 ,immune response ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Spinal cord injury (SCI) and spinal cord tumor are devastating events causing structural and functional impairment of the spinal cord and resulting in high morbidity and mortality; these lead to a psychological burden and financial pressure on the patient. These spinal cord damages likely disrupt sensory, motor, and autonomic functions. Unfortunately, the optimal treatment of and spinal cord tumors is limited, and the molecular mechanisms underlying these disorders are unclear. The role of the inflammasome in neuroinflammation in diverse diseases is becoming increasingly important. The inflammasome is an intracellular multiprotein complex and participates in the activation of caspase-1 and the secretion of pro-inflammatory cytokines such as interleukin (IL)-1β and IL-18. The inflammasome in the spinal cord is involved in the stimulation of immune-inflammatory responses through the release of pro-inflammatory cytokines, thereby mediating further spinal cord damage. In this review, we highlight the role of inflammasomes in SCI and spinal cord tumors. Targeting inflammasomes is a promising therapeutic strategy for the treatment of SCI and spinal cord tumors.
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- 2023
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9. A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration
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Yishan Chen, Yeke Yu, Ya Wen, Juan Chen, Junxin Lin, Zixuan Sheng, Wenyan Zhou, Heng Sun, Chengrui An, Jiansong Chen, Weiliang Wu, Chong Teng, Wei Wei, and Hongwei Ouyang
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Biology (General) ,QH301-705.5 ,Physiology ,QP1-981 - Abstract
Abstract Articular cartilage damage is a universal health problem. Despite recent progress, chondrocyte dedifferentiation has severely compromised the clinical outcomes of cell-based cartilage regeneration. Loss-of-function changes are frequently observed in chondrocyte expansion and other pathological conditions, but the characteristics and intermediate molecular mechanisms remain unclear. In this study, we demonstrate a time-lapse atlas of chondrocyte dedifferentiation to provide molecular details and informative biomarkers associated with clinical chondrocyte evaluation. We performed various assays, such as single-cell RNA sequencing (scRNA-seq), live-cell metabolic assays, and assays for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), to develop a biphasic dedifferentiation model consisting of early and late dedifferentiation stages. Early-stage chondrocytes exhibited a glycolytic phenotype with increased expression of genes involved in metabolism and antioxidation, whereas late-stage chondrocytes exhibited ultrastructural changes involving mitochondrial damage and stress-associated chromatin remodeling. Using the chemical inhibitor BTB06584, we revealed that early and late dedifferentiated chondrocytes possessed distinct recovery potentials from functional phenotype loss. Notably, this two-stage transition was also validated in human chondrocytes. An image-based approach was established for clinical use to efficiently predict chondrocyte plasticity using stage-specific biomarkers. Overall, this study lays a foundation to improve the quality of chondrocytes in clinical use and provides deep insights into chondrocyte dedifferentiation.
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- 2022
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10. The Effects of Drop Jump Height on Post-Activation Performance Enhancement at Different Ambient Temperatures
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Weiliang Wu, Zhizhou Chen, Chaoqun Chen, Dengpan Xue, Yuanyuan Lv, and Laikang Yu
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ambient temperature ,room temperature ,low temperature ,post-activation performance enhancement ,drop jump height ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
This study aimed to determine whether drop jump height will affect the post-activation performance enhancement (PAPE) effect at room temperature (RT) and low temperature (LT) conditions. Twelve male strength-trained males participated in this study. Prior to warm-up, subjects underwent a 30 min period of environmental adaptation. Different warm-up protocols were used at different ambient temperatures to help subjects achieve a level of muscle activity and body temperature similar to their daily training. After the pre-test, each subject participated in six experimental sessions at either RT or LT conditions, respectively, which were separated by at least 72 h and conducted at the same time of day to control for circadian influences on metabolism. After the conditioning activities, vertical jump (VJ) performance was re-tested at 4 min, 8 min, and 12 min of passive recovery to assess transient potentiation effects. Under RT conditions, 60 cm and 90 cm drop jumps could induce PAPE, with the PAPE effect being most significant at 4 min (p < 0.01) and 8 min (p < 0.01), respectively, while a 30 cm drop jump could not induce PAPE. Under LT conditions, 30 cm and 90 cm drop jumps could not induce PAPE, and VJ performance gradually declined over time (p < 0.01). However, although a 60 cm drop jump could not induce PAPE, VJ performance was not affected by LT at 8 min and declined at 12 min (p < 0.05). This study demonstrated that 60 cm and 90 cm drop jumps can be used to induce PAPE at RT conditions. LT can impair PAPE induction, while a 60 cm drop jump can be utilized for warm-up at LT conditions to counteract the effects of LT on athletic performance.
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- 2023
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11. Extracellular vesicles as novel therapeutic targets and diagnosis markers
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Yu Zhang, Weiliang Wu, Xiangbin Pan, Yanli Wang, Chengjie Wu, Lin Lu, Xi-Yong Yu, and Yangxin Li
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Extracellular vesicles ,ncRNA ,Diagnosis ,Therapeutic targets ,Biology (General) ,QH301-705.5 ,Medicine (General) ,R5-920 - Abstract
Extracellular vesicles (EVs) are membranous vesicles secreted from cells to the outside and are widely present in body fluids. The most commonly used method to isolate EVs is ultracentrifugation. EVs contain lipids, proteins, genetic material, and organelles, which can be used as communication carriers between different cells and potential disease biomarkers. EVs secreted by different cells (cancer cells, adipocytes, mesenchymal stem cells, immune cells et al.) play different functions. With the development of technology, EVs can also be engineered to deliver drugs. EVs also play an important role in the progression, prevention, diagnosis, and treatment of Corona Virus Disease 2019 (COVID-19).
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- 2022
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12. Bioaccessibility of methylmercury from marine fish commonly consumed in Guangdong Province and its application in dietary exposure assessment
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Ping WANG, Ruixia ZHAO, Zihui CHEN, Weiliang WU, Shaowei CHEN, Rui HUANG, Weilun HUO, Weixiong HUANG, and Xingfen YANG
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methylmercury ,bioaccessibility ,exposure assessment ,marine fish ,Food processing and manufacture ,TP368-456 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Objective To characterize methylmercury(MeHg) bioaccessibility from commonly consumed species of marine fish in Guangdong Province, and to investigate the effects of bioaccessibility on MeHg exposure of dietary marine fish. Methods A total of 36 samples of 12 species of marine fish were purchased from seafood trading market in Guangzhou City. The bioaccessibility of MeHg from raw, fried, grilled and steamed fish samples was determined by dual-phase in vitro gastrointestinal digestion based on the physiologically based extraction test. The bioavailability of MeHg in different sea fish and cooking method was compared, and bioaccessibility-corrected MeHg exposure assessment was conducted. Results The bioaccessibility of MeHg from raw fish ranged from 26.5%(Trichiurus lepturus) to 84.3%(Cynoglossus). Frying and grilling reduced MeHg bioaccessibility by 29.0% and 30.7%(P0.05). A significantly negative correlation was observed between initial MeHg concentration and MeHg bioaccessiblity(r=-0442, P
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- 2021
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13. Low concentration flufenamic acid enhances osteogenic differentiation of mesenchymal stem cells and suppresses bone loss by inhibition of the NF-κB signaling pathway
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Xuenan Liu, Zheng Li, Hao Liu, Yuan Zhu, Dandan Xia, Siyi Wang, Ranli Gu, Weiliang Wu, Ping Zhang, Yunsong Liu, and Yongsheng Zhou
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Flufenamic acid ,Mesenchymal stem cells ,Osteogenesis ,Osteoporosis ,Nuclear factor-κB ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background As the representative of fenamic acids, an important group of NSAIDs, flufenamic acid (FFA) has been used for anti-inflammation and analgesia in the clinic. Recently, researches have focused on the role of some members of NSAIDs in promoting osteogenesis. However, little attention has been paid to the subgroup of fenamic acids, and it remains unclear whether FFA and other fenamic acids could regulate mesenchymal stem cells’ (MSCs) lineage commitment and bone regeneration. Methods Here we treated two kinds of human MSCs with FFA at different concentrations in vitro and examined the effect of FFA on osteogenic differentiation of human MSCs. This was followed by heterotopic bone formation assay in nude mice. In addition, ovariectomized and aged mice were used as osteoporotic models to test the effect of FFA on osteoporosis. Besides, activators and inhibitor of nuclear factor-κB (NF-κB) signaling pathway and western blot were used to clarify the mechanism of the promoting effect of low concentration FFA on osteogenesis. Results Our results indicated that low concentrations of FFA could significantly enhance osteogenic differentiation of human MSCs in vitro, as well as in vivo. In addition, FFA treatment suppressed bone loss in ovariectomized and aged mice. Mechanistically, FFA at low concentrations promoted osteogenesis differentiation of human MSCs by inhibition of the NF-κB signaling pathway. Conclusions Collectively, our study suggested that low concentration FFA could be used in bone tissue engineering or osteoporosis by promoting osteogenic differentiation of human MSCs.
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- 2019
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14. Gene mining, codon optimization and analysis of binding mechanism of an aldo-keto reductase with high activity, better substrate specificity and excellent solvent tolerance.
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Wei Jiang, Xiaoli Fu, and Weiliang Wu
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Medicine ,Science - Abstract
The biosynthesis of chiral alcohols has important value and high attention. Aldo-keto reductases (AKRs) mediated reduction of prochiral carbonyl compounds is an interesting way of synthesizing single enantiomers of chiral alcohols due to the high enantio-, chemo- and regioselectivity of the enzymes. However, relatively little research has been done on characterization and apply of AKRs to asymmetric synthesis of chiral alcohols. In this study, the AKR from Candida tropicalis MYA-3404 (C. tropicalis MYA-3404), was mined and characterized. The AKR shown wider optimum temperature and pH. The AKR exhibited varying degrees of catalytic activity for different substrates, suggesting that the AKR can catalyze a variety of substrates. It is worth mentioning that the AKR could catalytic reduction of keto compounds with benzene rings, such as cetophenone and phenoxyacetone. The AKR exhibited activity on N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine (DKTP), a key intermediate for biosynthesis of the antidepressant drug duloxetine. Besides, the AKR still has high activity whether in a reaction system containing 10%-30% V/V organic solvent. What's more, the AKR showed the strongest stability in six common organic solvents, DMSO, acetonitrile, ethyl acetate, isopropanol, ethanol, and methanol. And, it retains more that 70% enzyme activity after 6 hours, suggesting that the AKR has strong solvent tolerance. Furthermore, the protein sequences of the AKR and its homology were compared, and a 3D model of the AKR docking with coenzyme NADPH were constructed. And the important catalytic and binding sites were identified to explore the binding mechanism of the enzyme and its coenzyme. These properties, predominant organic solvents resistance and extensive substrate spectrum, of the AKR making it has potential applications in the pharmaceutical field.
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- 2021
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15. Correction: A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration
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Yishan Chen, Yeke Yu, Ya Wen, Juan Chen, Junxin Lin, Zixuan Sheng, Wenyan Zhou, Heng Sun, Chengrui An, Jiansong Chen, Weiliang Wu, Chong Teng, Wei Wei, and Hongwei Ouyang
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Biology (General) ,QH301-705.5 ,Physiology ,QP1-981 - Published
- 2022
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16. Silicon based solar cells using a multilayer oxide as emitter
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Jie Bao, Weiliang Wu, Zongtao Liu, and Hui Shen
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Physics ,QC1-999 - Abstract
In this work, n-type silicon based solar cells with WO3/Ag/WO3 multilayer films as emitter (WAW/n-Si solar cells) were presented via simple physical vapor deposition (PVD). Microstructure and composition of WAW/n-Si solar cells were studied by TEM and XPS, respectively. Furthermore, the dependence of the solar cells performances on each WO3 layer thickness was investigated. The results indicated that the bottom WO3 layer mainly induced band bending and facilitated charge-carriers separation, while the top WO3 layer degraded open-circuit voltage but actually improved optical absorption of the solar cells. The WAW/n-Si solar cells, with optimized bottom and top WO3 layer thicknesses, exhibited 5.21% efficiency on polished wafer with area of 4 cm2 under AM 1.5 condition (25 °C and 100 mW/cm2). Compared with WO3 single-layer film, WAW multilayer films demonstrated better surface passivation quality but more optical loss, while the optical loss could be effectively reduced by implementing light-trapping structures. These results pave a new way for dopant-free solar cells in terms of low-cost and facile process flow.
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- 2016
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17. Abdominal Muscle Activity during Mechanical Ventilation Increases Lung Injury in Severe Acute Respiratory Distress Syndrome.
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Xianming Zhang, Weiliang Wu, Yongcheng Zhu, Ying Jiang, Juan Du, and Rongchang Chen
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Medicine ,Science - Abstract
It has proved that muscle paralysis was more protective for injured lung in severe acute respiratory distress syndrome (ARDS), but the precise mechanism is not clear. The purpose of this study was to test the hypothesis that abdominal muscle activity during mechanically ventilation increases lung injury in severe ARDS.Eighteen male Beagles were studied under mechanical ventilation with anesthesia. Severe ARDS was induced by repetitive oleic acid infusion. After lung injury, Beagles were randomly assigned into spontaneous breathing group (BIPAPSB) and abdominal muscle paralysis group (BIPAPAP). All groups were ventilated with BIPAP model for 8h, and the high pressure titrated to reached a tidal volume of 6ml/kg, the low pressure was set at 10 cmH2O, with I:E ratio 1:1, and respiratory rate adjusted to a PaCO2 of 35-60 mmHg. Six Beagles without ventilator support comprised the control group. Respiratory variables, end-expiratory volume (EELV) and gas exchange were assessed during mechanical ventilation. The levels of Interleukin (IL)-6, IL-8 in lung tissue and plasma were measured by qRT-PCR and ELISA respectively. Lung injury scores were determined at end of the experiment.For the comparable ventilator setting, as compared with BIPAPSB group, the BIPAPAP group presented higher EELV (427±47 vs. 366±38 ml) and oxygenation index (293±36 vs. 226±31 mmHg), lower levels of IL-6(216.6±48.0 vs. 297.5±71.2 pg/ml) and IL-8(246.8±78.2 vs. 357.5±69.3 pg/ml) in plasma, and lower express levels of IL-6 mRNA (15.0±3.8 vs. 21.2±3.7) and IL-8 mRNA (18.9±6.8 vs. 29.5±7.9) in lung tissues. In addition, less lung histopathology injury were revealed in the BIPAPAP group (22.5±2.0 vs. 25.2±2.1).Abdominal muscle activity during mechanically ventilation is one of the injurious factors in severe ARDS, so abdominal muscle paralysis might be an effective strategy to minimize ventilator-induce lung injury.
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- 2016
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18. Correction: Abdominal Muscle Activity during Mechanical Ventilation Increases Lung Injury in Severe Acute Respiratory Distress Syndrome.
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Xianming Zhang, Weiliang Wu, Yongcheng Zhu, Ying Jiang, Juan Du, and Rongchang Chen
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Medicine ,Science - Published
- 2016
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19. The orchestration of cell-cycle reentry and ribosome biogenesis network is critical for cardiac repair.
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Yanli Wang, Junchu Tu, Weiliang Wu, Yan Xu, Yujie Li, Xiangbin Pan, Bin Liu, Tonggan Lu, Qingfang Han, Huiling Zhang, Lijuan Jiao, Yu Zhang, Xi-Yong Yu, Zhenya Shen, and Yangxin Li
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- 2024
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20. A 120-kW electric vehicle DC charger with two charging guns
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Weiliang Wu and Xiping Liu
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Applied Mathematics ,Electrical and Electronic Engineering - Published
- 2023
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21. Atomic scale controlled tunnel oxide enabled by a novel industrial tube‐based PEALD technology with demonstrated commercial TOPCon cell efficiencies > 24%
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Baochen Liao, Weiliang Wu, Reuben J. Yeo, Xinyuan Wu, Sheng Ma, Qiang Wang, Yimao Wan, Xiaodong Su, Wenzhong Shen, Xiang Li, Weimin Li, Guoqiang Xing, and Bram Hoex
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Renewable Energy, Sustainability and the Environment ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2022
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22. Electromagnetic Characteristic Analysis and Optimization of a Novel Reverse Salient PMSM for Wide Speed Range.
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Ruipan Lu, Zhangqi Liu, Xiping Liu, Jianwei Liang, Weiliang Wu, and Wenrui Wang
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To address the issues associated with the conventional permanent magnet synchronous machine, particularly difficulties in adjusting the air-gap flux barrier and the limited range of constant power speed regulation, this paper introduces a novel approach. It combines the intensifying-flux effect with the permanent magnet synchronous machine to propose a new design known as the reverse salient permanent magnet synchronous machine (RS-PMSM) with a multilayered flux barrier. This innovation serves to enhance the working performance of the permanent magnets. The paper's structure includes an initial introduction to the RS-PMSM, outlining its structure and operational principles. Following this, an optimization approach employing NSGA-II is used to define the RS-PMSM's optimization model. The objectives of this optimization encompass torque, torque ripple, and the reverse salient pole ratio. The study then proceeds to conduct a comprehensive set of performance analyses and comparisons, involving the initial machine, optimized machine, and a conventional machine. The performance metrics considered include no-load air-gap flux density, reverse electromotive force, torque characteristics, speed range, and efficiency. Finally, the study verifies the design rationality of the RS-PMSM, highlighting its potential to address the challenges posed by traditional permanent magnet synchronous motors. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Tube‐type plasma‐enhanced atomic layer deposition of aluminum oxide: Enabling record lab performance for the industry with demonstrated cell efficiencies >24%
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Baochen Liao, Xinyuan Wu, Weiliang Wu, Changming Liu, Sheng Ma, Shaozhou Wang, Tong Xie, Qiang Wang, Zheren Du, Wenzhong Shen, Xiang Li, Weimin Li, and Bram Hoex
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Renewable Energy, Sustainability and the Environment ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2022
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24. A DC Charging Pile for New Energy Electric Vehicles
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Weiliang Wu, Xiping Liu, and Chaozhi Huang
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Electrical and Electronic Engineering - Published
- 2023
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25. Cover Image
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Baochen Liao, Weiliang Wu, Reuben J. Yeo, Xinyuan Wu, Sheng Ma, Qiang Wang, Yimao Wan, Xiaodong Su, Wenzhong Shen, Xiang Li, Weimin Li, Guoqiang Xing, and Bram Hoex
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Renewable Energy, Sustainability and the Environment ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2023
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26. Cover Image
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Baochen Liao, Xinyuan Wu, Weiliang Wu, Changming Liu, Sheng Ma, Shaozhou Wang, Tong Xie, Qiang Wang, Zheren Du, Wenzhong Shen, Xiang Li, Weimin Li, and Bram Hoex
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Renewable Energy, Sustainability and the Environment ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2022
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27. Suppression of potential-induced degradation in monofacial PERC solar cells with gradient-designed capping layer
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Lan Wang, Li Junjun, Tao Chen, Yuelong Huang, Jian Yu, Weiliang Wu, He Jialong, Li Bingchuan, Su Rong, Pu Wang, and Xianhui Cui
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Materials science ,Silicon oxynitride ,Renewable Energy, Sustainability and the Environment ,business.industry ,Potential induced degradation ,chemistry.chemical_compound ,Surface coating ,Silicon nitride ,chemistry ,Optoelectronics ,General Materials Science ,Crystalline silicon ,business ,Silicon oxide ,p–n junction ,Common emitter - Abstract
The potential-induced degradation (PID) of p-type crystalline silicon passivated emitter and rear cell (PERC) is a critical issue causing severe output power loss. In this paper, a gradient-designed capping layer consisting of silicon nitride/silicon oxynitride/silicon oxide (SiNx/SiNxOy/SiOx) is proposed as the surface coating for p-type PERC solar cells. The capping layer improves the short wavelength spectral response due to its better anti-reflection effect. An impressive efficiency of 23.52% has been achieved on mass production line. A long-term PID evolution up to 672 h is performed for glass/back sheet PERC cell modules under stringent conditions with the ambient temperature of 85 °C, relative humidity of 85%, and the applied voltage of 1500 V. The current–voltage (light I-V, dark I-V) parameters of the modules were measured, as well as the electroluminescence, to evaluate the effect of sodium ion migration in PERC solar cells. It is shown that monofacial PERC solar modules suffer from shunt of PN junction under PID stress, which can be obviously suppressed by gradient-designed capping layers. The optimized PERC solar modules show excellent PID resistance, showing great potential for lower levelized cost of energy (LCOE) of photovoltaic power generation.
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- 2021
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28. Drop Analyses and Verification of the Spent Nuclear Fuel Transportation Cask Under Hypothetical Accident Conditions
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Guangdong Liu, Weiliang Wu, He Zhu, Qingjun Ma, and Qipeng Li
- Abstract
According to IAEA regulations, the spent nuclear fuel transportation cask must be able to withstand a free drop from the height of 9 meters on an unyielding surface. The impact limiter of the cask absorbs most of the impact energy and reduces the deceleration loads on the cask and contents. In order to accurately estimate the safety of the cask and the spent nuclear fuel, the calculation of drop acceleration is very important. In this study, based on the absorption of energy by an impact limiter is equal to the work done in crushing a volume, the maximum deceleration is estimated during collision process between cask and the ground. Then using the LS-DYNA computer program to simulate the cask drop behavior of 9 meters free drop. By comparing the results of theoretical analysis, numerical simulation and actual drop tests, the cask maximum acceleration and the impact limiter deformation are in good agreement, which prove the validity of the spent nuclear fuel transportation cask drop analysis. The stress intensity results for 9 meters free drop loading cases satisfy the allowable stress limit criteria as presented in “ASME Boiler and Pressure Vessel Code”. The spent nuclear fuel transportation cask meets the requirements of IAEA in the 9m free drop hypothetical accident conditions.
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- 2022
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29. circHIPK3 prevents cardiac senescence by acting as a scaffold to recruit ubiquitin ligase to degrade HuR
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Fengzhi Ding, Lin Lu, Chengjie Wu, Xiangbin Pan, Bin Liu, Yu Zhang, Yanli Wang, Weiliang Wu, Bing Yan, Yuqing Zhang, Xi-Yong Yu, and Yangxin Li
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Heart Diseases ,Ubiquitin ,Ubiquitin-Protein Ligases ,Medicine (miscellaneous) ,RNA, Circular ,beta-Transducin Repeat-Containing Proteins ,Mice ,Animals ,Humans ,RNA ,Myocytes, Cardiac ,RNA, Messenger ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Cellular Senescence - Published
- 2022
30. A promising insight into the inhibition of lipid oxidation, protein degradation and biogenic amine accumulation in postmortem fish: Functional glazing layers of modified bio-polymer
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Xiaodan Hui, Yu Wan, Hao Dong, Jian Peng, Weiliang Wu, Xingfen Yang, and Qi He
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Food Science - Published
- 2023
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31. A FEW EQUIVALENT STATEMENTS OF A HILBERT-TYPE INTEGRAL INEQUALITY WITH THE RIEMANN-ZETA FUNCTION
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Bicheng Yang and Weiliang Wu
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Pure mathematics ,Weight function ,symbols.namesake ,Operator (computer programming) ,Real analysis ,Plane (geometry) ,General Mathematics ,symbols ,Function (mathematics) ,Expression (computer science) ,Type (model theory) ,Mathematics ,Riemann zeta function - Abstract
By using the way of real analysis and the weight functions, a few equivalent statements of a Hilbert-type integral inequality in the whole plane with the internal variables and the best possible constant factor related to the Riemann-zeta function is proved. The operator expression and some particular cases are considered.
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- 2020
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32. Associations of long-term cadmium exposure with peripheral white blood cell subtype counts and indices in residents of cadmium-polluted areas
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Lili Xiong, Cuihua Fan, Jia Song, Yu Wan, Xiuqin Lin, Zujian Su, Jianmin Qiu, WeiLiang Wu, Zhini He, Yongning Wu, and Xingfen Yang
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Leukocyte Count ,Environmental Engineering ,Cross-Sectional Studies ,Neutrophils ,Health, Toxicology and Mutagenesis ,Creatinine ,Public Health, Environmental and Occupational Health ,Environmental Chemistry ,Humans ,General Medicine ,General Chemistry ,Pollution ,Cadmium - Abstract
Experimental evidence suggests that exposure to cadmium (Cd) could affect immune cells in vivo and in vitro. However, the associations of long-term Cd exposure with white blood cell (WBC) subtype counts and hemogram-derived indices have been rarely investigated. Therefore, we evaluated these relationships in residents of cadmium-polluted areas.This cross-sectional study included 431 participants aged 45-75 years without occupational exposure histories from Cd-contaminated areas of southern China. We detected WBC, neutrophil, lymphocyte, and monocyte counts using routine blood tests and calculated neutrophil-lymphocyte ratio (NLR), systemic inflammation response index (SIRI), and lymphocyte-monocyte ratio (LMR). Urinary Cd (U-Cd) was measured with inductively coupled plasma mass spectrometry and adjusted for creatinine. To evaluate the associations of U-Cd with peripheral WBC subtype counts and indices, we performed multivariate linear regression, logistic regression and subgroup analyses using U-Cd categorized into quartiles.In models adjusted for all potential confounders, U-Cd was negatively associated with WBC, neutrophil, and monocyte counts in Q2, compared with Q1 of U-Cd (p 0.05). A similar relationship was observed between U-Cd and NLR and SIRI, whereas the corresponding association for LMR was positive (p 0.05). In subgroup analyses, U-Cd was negatively associated with neutrophil count, except for never smokers, after full adjustment.U-Cd was negatively associated with WBC count, neutrophil count, monocyte count, NLR, and SIRI, and positively associated with LMR. Therefore, neutrophil count could be a potential indicator of long-term Cd exposure-associated immunosuppressive effect.
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- 2022
33. Effect of dexamethasone on levels of inflammatory factors and EGF mRNA in rabbits suffering from oral ulcers
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Weiliang Wu, Jianyong Ruan, Daxu Li, Hong Tao, Chunni Deng, Rui Wang, Wei Cheng, Zhe Zhang, and Le Ren
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Pharmaceutical Science ,Pharmacology (medical) ,Oral ulcer, Dexamethasone, Inflammatory factors, Epidermal growth factors - Abstract
Purpose: To investigate the therapeutic effect of dexamethasone on rabbits suffering from oral ulcers, and the underlying mechanism(s) of action. Methods: A rabbit model of oral ulcer was established by applying 40 % glacial acetic acid solution to the oral buccal membranes of the animals. Three groups of rabbits were used. Changes in area of the oral ulcer were recorded after dexamethasone administration. Levels of epidermal growth factor (EGF) were assayed using reverse transcription PCR (RT-PCR), while MDA levels and expression levels of IL- 6, IL-8 and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA). Local histopathological changes were examined histologically with the aid of hematoxylin and eosin (H & E) staining. Results: There were reductions in ulcer areas in group C on the 2nd, 4th and 7th days of dexamethasone administration, when compared with group B (p < 0.05). The EGF levels in the buccal mucosa of rabbits in groups B and C were significantly higher than those in group A (p < 0.05), while the highest EGF level was in group C (p < 0.05). The levels of MDA, IL-6, IL-8, and TNF-α significantly increased in groups B and C (p < 0.05). Results from H & E staining showed lower levels of inflammatory cells in group C than in group B, with visible proliferation of fibroblast cells and epithelial cells in group C after dexamethasone administration. Conclusion: Dexamethasone accelerates healing of oral ulcer by regulating EGF levels. This finding provides a new approach to the treatment of oral ulcers.
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- 2022
34. Hyperoside attenuates Cd-induced kidney injury via inhibiting NLRP3 inflammasome activation and ROS/MAPK/NF-κB signaling pathway in vivo and in vitro
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Ziyin Li, Weizuo Liao, Xinxin Yin, Lili Liu, Zhiqiang Zhao, Xiaodan Lu, Feifei Xu, Xiuqin Lin, Yingsi Chen, Jia Song, Zhini He, Qinzhi Wei, Weiliang Wu, Yongning Wu, and Xingfen Yang
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General Medicine ,Toxicology ,Food Science - Abstract
Cadmium accumulates in the kidney and causes inflammation. The NLRP3 inflammasome has been linked to the pathogenesis of inflammation. Hyperoside (HYP) possesses potent nephroprotective properties against of kidney injury. This study aimed to research the effects and related mechanism of HYP on Cd-induced kidney damage. Wide-type and NLRP3
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- 2023
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35. Exploration And Practice Of Blended Teaching Based On Mobile Internet And Matlab : Taking The Course Of Mathematical Analysis As Example
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Yunhu Ye and Weiliang Wu
- Published
- 2021
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36. Reproducibility of fluid-phase measurements in PBS-treated sputum supernatant of healthy and stable COPD subjects
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Lili Guan, Kuimiao Deng, Luqian Zhou, Jianhua Chen, Weiliang Wu, Yuqiong Yang, Fengyan Wang, Mei Jiang, Weijuan Shi, Rongchang Chen, and Zhenyu Liang
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COPD ,biology ,business.industry ,Autoantibody ,General Medicine ,medicine.disease ,Molecular biology ,Cytokeratin ,Antigen ,biology.protein ,medicine ,Sputum ,Tumor necrosis factor alpha ,Interleukin 8 ,medicine.symptom ,business ,Heat shock protein 47 - Abstract
Purpose The purpose of this study was to investigate the reproducibility of fluid-phase measurements in PBS-treated sputum supernatant, processed using the two-step method, of healthy and stable COPD individuals. Methods Nine healthy subjects and 23 stable COPD patients provided sputum twice within 6 days. A two-step sputum processing method was used to obtain PBS-treated supernatant and sputum cells. Soluble protein markers and IgG and IgM autoantibody profiles in PBS supernatant were analyzed using customized microarrays. Repeatability of measurements was assessed by paired-sample testing and an intraclass correlation coefficient, then graphically reported by Bland-Altman plot. Results There was no significant difference between the repeated detection of 8/10 types of soluble protein markers, all 13 types of IgG autoantibodies, and 12/13 types of corresponding IgM autoantibodies in PBS supernatant. The repeatability of measurements in PBS supernatant was substantial to very good for interleukin 6 (IL6), IL8, IL13, IL10, IL33, vascular endothelial growth factor, soluble receptor for advanced glycation end-products, and tumor necrosis factor-α; for IgG autoantibodies against aggrecan, centromere protein B (CENP-B), collagen II, collagen IV, cytochrome C, elastin, heat shock protein 47 (HSP47), HSP70, and La/Sjogren syndrome type B antigen; for IgM autoantibodies against CENP-B, collagen I, collagen II, collagen IV, cytokeratin 18, and HSP70; and for sputum neutrophils, macrophages and eosinophils count. Bland-Altman plots suggested good consistency within repeated measurements. Stable COPD patients differed from healthy subjects in the proportion of neutrophils and eosinophils; relative fluorescence intensity of anti-cytochrome C IgG, anti-aggrecan IgM, and anti-cytochrome C IgM. There was a significant positive correlation for stable COPD patients between sputum anti-collagen II IgG and post-bronchodilator FEV1%. Conclusion We confirmed fluid-phase measurements in PBS-treated sputum supernatant by high-throughput techniques with good repeatability. We demonstrated the presence of IgG and IgM autoantibodies to multiple antigens in the airways of COPD patients.
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- 2019
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37. Food fraud vulnerability assessment in the edible vegetable oil supply chain: A perspective of Chinese enterprises
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Zhaohui Yang, Quan Zhou, Weiliang Wu, Duo Zhang, Lifen Mo, Jiali Liu, and Xingfen Yang
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Food Science ,Biotechnology - Published
- 2022
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38. High-resolution architecture of human epiphysis formation
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Jiansong Chen, Weiliang Wu, Chengrui An, Tian Qin, Heng Sun, Hongwei Ouyang, Ya Wen, Ting Gang Chew, Chunmei Fan, Junfeng Ji, and Yishan Chen
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medicine.anatomical_structure ,Ossification ,Epiphysis ,Regeneration (biology) ,Cartilage ,medicine ,High resolution ,medicine.symptom ,Ossification center ,Biology ,Endochondral ossification ,Chondrocyte ,Cell biology - Abstract
SummaryHuman limb skeletal system consists of both bone and cartilage which originated from fetal cartilage. However, the roadmap of chondrocyte divergent differentiation to bone and articular cartilage has yet to be established. Epiphysis possesses articular cartilage, growth plate and the secondary ossification center (SOC), making it an ideal model to uncover the trajectory of chondrocyte divergent differentiation. Here, we mapped differentiation trajectory of human chondrocyte during postnatal finger epiphysis development by using single-cell RNA sequencing. Our results uncovered that chondroprogenitors have two differentiation pathways to hypertrophic chondrocytes during ossification, and one pathway to articular chondrocytes for formation of cartilages. Interestingly, we found that, as an addition to the known typical endochondral ossification path from resting, proliferative to hypertrophic chondrocytes, there was a bypass by which chondroprogenitors differentiate into hypertrophic chondrocytes without proliferative stage. Furthermore, our results revealed two new chondrocyte subpopulations (bypass chondrocytes as it appeared in the ossification bypass, and ID1+ chondroblasts in articular chondrocyte path) during postnatal epiphysis development in addition to six well-known subpopulations. Overall, our study provides a comprehensive roadmap of chondrocyte differentiation in human epiphysis thereby expanding the knowledge of bone and articular cartilage, which could be utilized to design biotherapeutics for bone and articular cartilage regeneration.
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- 2020
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39. Characterization and Catalytic-Site-Analysis of an Aldo-Keto Reductase with Excellent Solvent Tolerance
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Weiliang Wu, Shu-Feng Zhou, Libing Tian, Wei Jiang, Pei Rui, and Yuqian Zhang
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0301 basic medicine ,enzyme assay ,Acetylacetone ,Reductase ,lcsh:Chemical technology ,01 natural sciences ,Catalysis ,lcsh:Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,aldo-keto reductase ,lcsh:TP1-1185 ,Physical and Theoretical Chemistry ,organic solvents ,substrate spectrum ,010405 organic chemistry ,Enantioselective synthesis ,Substrate (chemistry) ,Combinatorial chemistry ,0104 chemical sciences ,Solvent ,030104 developmental biology ,chemistry ,lcsh:QD1-999 ,Methanol ,Enantiomer ,chiral alcohol - Abstract
Aldo-keto reductases (AKRs) mediated stereoselective reduction of prochiral carbonyl compounds is an efficient way of preparing single enantiomers of chiral alcohols due to their high chemo-, enantio-, and regio-selectivity. To date, the application of AKRs in the asymmetric synthesis of chiral alcohols has been limited, due to the challenges of cloning and purifying. In this work, the aldo-keto reductase (AKR3-2-9) from Bacillus sp. was obtained, purified and proved to be NADPH-dependent. It exhibits good bioactivity and stability at 37 °, C, pH 6.0. AKR3-2-9 is catalytically active on 11 pairs of substrates such as 3-methylcyclohexanone and methyl pyruvate, among which it showed the highest catalytic activity for acetylacetone. In addition, AKR3-2-9 was able to be resistant to five common organic solvents such as methanol and ethanol, it retained high catalytic activity even in a reaction system containing 10% v/v organic solvent for 6 h, which indicates its broad substrate spectrum and exceptional organic solvent tolerance. Furthermore, its three-dimensional structure was constructed and catalytic-site-analysis of the enzyme was conducted. Notably, it was capable of catalyzing the reaction of the key intermediates of duloxetine. The extensive substrate spectrum and predominant organic solvents resistance makes AK3-2-9 a promising enzyme which can be potentially applied in medicine synthesis.
- Published
- 2020
40. 疫情严重地区居民对COVID-19的知识、态度、行为调查
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Youkun, Hu, Gaoqiang, Zhang, Zhihao, Li, Jiani, Yang, Lijun, Mo, Xiru, Zhang, Lili, Xiong, Weiliang, Wu, Chen, Mao, and Xingfen, Yang
- Subjects
Betacoronavirus ,Attitude ,SARS-CoV-2 ,Pneumonia, Viral ,COVID-19 ,Humans ,Female ,Coronavirus Infections ,Pandemics ,流行病学调查 - Abstract
OBJECTIVE: To survey the residents for their understanding of Coronavirus Disease 2019 (COVID-19)-related knowledge, attitude and practices (KAP) in two hard hit provinces of China to facilitate the governmental decisions on strategies against the disease. METHODS: We invited the participants from Hubei and Henan Provinces of China for an internetbased survey starting from 12:00 on February 21, 2020 to 12:00 on February 23. The survey included the general conditions, KAP of COVID-19, psychological status and living conditions of the residents. RESULTS: The effective response rate of the questionnaire was 98.9%. The mean (P25, P75) age of the participants was 19 (16, 40) years, and 54.3% of them were students. Social media were the most important source of information concerning the pandemic of the respondents. The respondents had a high awareness of person-to-person transmission of the virus through the respiratory tract or droplets but showed a relatively low level of awareness of the population susceptible to COVID-19 and its specific symptoms. The results of multivariate analysis showed that women, undergraduate students (including college students) and higher degree holders had better knowledge of COVID-19 (P < 0.05); the proportion of respondents who expressed to have different levels of psychological stressed such as worry, anxiety and panic reached 77.2%; 16.7% of the responders considered psychological interventions necessary for their psychological conditions; 63.6% of the respondents confessed a bias against the people returning from Hubei and Henan provinces, while 22.4% worried that they might be biased because of their residence in Hubei and Henan. The rate of personal protective equipment shortage was as high as 69.4%; the rates of the responders who would "covering the mouth and nose when coughing or sneezing", "properly use masks in accordance with regulations", "maintain proper hand hygiene ", "avoid gatherings with relatives and friends" and "refrain from going to public places" were 92.4%, 95.9%, 93.5%, 88.8% and 93.1%, respectively. Women and groups with good knowledge of the disease reported better protective behaviors against the diseases (P < 0.05). CONCLUSIONS: The residents in Hubei and Henan Provinces have generally good KAP related to COVID-19, and the online platforms plays a positive role to in circulating epidemic-related information. It is essential to further increase the supply of the protective materials and pay more attention to the mental health of the residents during the pandemic, and psychological counseling and psychological protection should be provided if necessary.
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- 2020
41. Novel hole selective CrOx contact for dopant-free back contact silicon solar cells
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Zhirong Yao, Bao Jie, Zongcun Liang, Zongtao Liu, Lun Cai, Hui Shen, Youjun Deng, Kaifu Qiu, Wenjie Lin, and Weiliang Wu
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Materials science ,Silicon ,chemistry.chemical_element ,02 engineering and technology ,01 natural sciences ,law.invention ,chemistry.chemical_compound ,law ,0103 physical sciences ,Solar cell ,General Materials Science ,Work function ,Common emitter ,010302 applied physics ,Chromium trioxide ,Dopant ,business.industry ,Mechanical Engineering ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Ambient air ,Silicon based ,chemistry ,Mechanics of Materials ,Optoelectronics ,0210 nano-technology ,business - Abstract
The dopant-free back contact solar cells are demonstrated based on hole selective contact material, chromium trioxide (CrO x , x x is first applied in silicon based solar cell as the emitter. Integrating 5 nm CrO x and 2 nm LiF x into solar cell as the emitter and the back surface filed, resulted in a device efficiency of 13.6%. For further improvement, the back contact solar cell reaching an efficiency of 15.8% was fabricated, by implementing the multilayer films of CrO x (5 nm)/Au (4 nm)/ CrO x (5 nm) as the emitter. The formation of Cr(OH) 3 in ambient condition, resulted in a lower work function (4.8 eV) of the CrO x film. Furthermore, the multilayer back contact solar cell demonstrated a high stability due to CrO x covered with 500 nm Ag, when stored in ambient air longer than 170 days.
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- 2018
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42. Chinese version of the severe respiratory insufficiency questionnaire for patients with COPD receiving long-term oxygen therapy
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Xiaoying Li, Jiawen Xu, Yating Huo, Bingpeng Guo, Lili Guan, Luqian Zhou, Weiliang Wu, Yuqiong Yang, Jianhua Chen, and Rongchang Chen
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Male ,medicine.medical_specialty ,China ,Time Factors ,Concurrent validity ,International Journal of Chronic Obstructive Pulmonary Disease ,Severity of Illness Index ,chronic obstructive pulmonary disease ,severe respiratory insufficiency questionnaire ,03 medical and health sciences ,Pulmonary Disease, Chronic Obstructive ,0302 clinical medicine ,Quality of life ,Cronbach's alpha ,Cost of Illness ,Predictive Value of Tests ,Surveys and Questionnaires ,Positive airway pressure ,medicine ,Content validity ,Humans ,030212 general & internal medicine ,Prospective Studies ,Lung ,Original Research ,Aged ,COPD ,business.industry ,Oxygen Inhalation Therapy ,Construct validity ,Reproducibility of Results ,General Medicine ,Middle Aged ,Translating ,medicine.disease ,long-term oxygen therapy ,Confirmatory factor analysis ,health-related quality of life ,Treatment Outcome ,030228 respiratory system ,Physical therapy ,Quality of Life ,Female ,business ,Respiratory Insufficiency - Abstract
Lili Guan,1,* Jiawen Xu,1,* Weiliang Wu,1,* Jianhua Chen,1,* Xiaoying Li,2,* Bingpeng Guo,1 Yuqiong Yang,1 Yating Huo,1 Luqian Zhou,1 Rongchang Chen1 1State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; 2The First Affiliated Hospital, School of Clinical Medicine of Guangdong Pharmaceutical University, Guangzhou, China *These authors contributed equally to this work Purpose: Patients with advanced-stage COPD often experience severe hypoxemia. Treatment with long-term oxygen therapy (LTOT) may relieve patients’ symptoms and increase survival. As COPD is incurable, improving patients’ health-related quality of life is important. The Chinese version of the Severe Respiratory Insufficiency Questionnaire (SRI) is valid for patients with hypercapnic COPD undergoing noninvasive positive airway pressure ventilation at home. However, the reliability and validity of the Chinese SRI for patients with COPD undergoing LTOT have not been investigated. Patients and methods: We analyzed reliability using Cronbach’s α coefficient. Construct validity was assessed with principal, exploratory, and confirmatory factor analysis. Concurrent validity was evaluated through the correlation between SRI domains and Chronic Respiratory Disease Questionnaire (CRQ) domains. Content validity was assessed by calculating the correlation between each SRI item score and the total score for the relevant domain. Results: In total, 161 patients participated in this study. The Cronbach’s α coefficient for all SRI domains was >0.7, except for the attendant symptoms and sleep domain. Exploratory and confirmatory factor analysis showed a good model fit for each domain, but the factors extracted from each domain were correlated. SRI and CRQ domains correlated well with respect to similar aspects of health-related quality of life, indicating good concurrent validity. Content validity was indirectly shown by a good correlation between each item score and the total score of the relevant domain. Conclusion: The Chinese version of the SRI has a good reliability and validity for patients with COPD undergoing LTOT in China. Keywords: severe respiratory insufficiency questionnaire, long-term oxygen therapy, health-related quality of life, chronic obstructive pulmonary disease
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- 2018
43. Efficiency enhancement of bifacial PERC solar cells with laser-doped selective emitter and double-screen-printed Al grid
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Weiliang Wu, Wenjie Lin, Zhongwei Zhang, Hui Shen, Zongcun Liang, and Fei Zheng
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Materials science ,Renewable Energy, Sustainability and the Environment ,business.industry ,020209 energy ,Doping ,02 engineering and technology ,Condensed Matter Physics ,Laser ,Grid ,Electronic, Optical and Magnetic Materials ,law.invention ,law ,0202 electrical engineering, electronic engineering, information engineering ,Optoelectronics ,Crystalline silicon ,Electrical and Electronic Engineering ,business ,Common emitter - Published
- 2018
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44. Gene mining, codon optimization and analysis of binding mechanism of an aldo-keto reductase with high activity, better substrate specificity and excellent solvent tolerance
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Xiaoli Fu, Weiliang Wu, and Wei Jiang
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viruses ,Aldo-Keto Reductases ,Coenzymes ,Biochemistry ,Substrate Specificity ,chemistry.chemical_compound ,Computational Chemistry ,hemic and lymphatic diseases ,Enzyme Chemistry ,Materials ,chemistry.chemical_classification ,Multidisciplinary ,biology ,Organic Compounds ,Chemistry ,Biochemical Cofactors ,Chemical Synthesis ,Ketones ,Enzymes ,Molecular Docking ,Solutions ,Physical Sciences ,Medicine ,Organic Solvents ,Research Article ,Stereochemistry ,Science ,Materials Science ,Biosynthesis ,Research and Analysis Methods ,Catalysis ,Cofactor ,Fungal Proteins ,Candida tropicalis ,Codon ,Aldo-keto reductase ,Binding Sites ,Organic Chemistry ,Chemical Compounds ,Enantioselective synthesis ,Biology and Life Sciences ,Proteins ,Substrate (chemistry) ,Enzyme ,Docking (molecular) ,Alcohols ,Mixtures ,Solvents ,Enzymology ,biology.protein ,Enantiomer ,Acids - Abstract
The biosynthesis of chiral alcohols has important value and high attention. Aldo–keto reductases (AKRs) mediated reduction of prochiral carbonyl compounds is an interesting way of synthesizing single enantiomers of chiral alcohols due to the high enantio-, chemo- and regioselectivity of the enzymes. However, relatively little research has been done on characterization and apply of AKRs to asymmetric synthesis of chiral alcohols. In this study, the AKR from Candida tropicalis MYA-3404 (C. tropicalis MYA-3404), was mined and characterized. The AKR shown wider optimum temperature and pH. The AKR exhibited varying degrees of catalytic activity for different substrates, suggesting that the AKR can catalyze a variety of substrates. It is worth mentioning that the AKR could catalytic reduction of keto compounds with benzene rings, such as cetophenone and phenoxyacetone. The AKR exhibited activity on N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine (DKTP), a key intermediate for biosynthesis of the antidepressant drug duloxetine. Besides, the AKR still has high activity whether in a reaction system containing 10%-30% V/V organic solvent. What’s more, the AKR showed the strongest stability in six common organic solvents, DMSO, acetonitrile, ethyl acetate, isopropanol, ethanol, and methanol. And, it retains more that 70% enzyme activity after 6 hours, suggesting that the AKR has strong solvent tolerance. Furthermore, the protein sequences of the AKR and its homology were compared, and a 3D model of the AKR docking with coenzyme NADPH were constructed. And the important catalytic and binding sites were identified to explore the binding mechanism of the enzyme and its coenzyme. These properties, predominant organic solvents resistance and extensive substrate spectrum, of the AKR making it has potential applications in the pharmaceutical field.
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- 2021
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45. Improved indium oxide transparent conductive thin films by hydrogen annealing
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Weiliang Wu, Lun Cai, Wang Xuemeng, Kaifu Qiu, Hui Shen, Shenghao Li, Bing Gao, and Zhirong Yao
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010302 applied physics ,Materials science ,Hydrogen ,Annealing (metallurgy) ,Mechanical Engineering ,Metallurgy ,Oxide ,Recrystallization (metallurgy) ,chemistry.chemical_element ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,X-ray photoelectron spectroscopy ,Mechanics of Materials ,0103 physical sciences ,General Materials Science ,Thin film ,0210 nano-technology ,Forming gas ,Indium - Abstract
In 2 O 3 thin films were prepared by reactive evaporation with post-annealing treatment at forming gas of N 2 and H 2 . After post-annealing treatment, the films annealed at forming gas achieved lowest resistivity of 2.70 × 10 −4 Ω cm. The XRD results show that the In 2 O 3 films annealed in forming gas and N 2 with good recrystallization. In the forming gas annealing process, hydrogen plays a key role in the generation of oxygen vacancies and enhances the formation of In–OH bond which may influence the electrical and optical properties of the In 2 O 3 films.
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- 2017
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46. Correlation and compatibility between surface respiratory electromyography and transesophageal diaphragmatic electromyography measurements during treadmill exercise in stable patients with COPD
- Author
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Rongchang Chen, Lili Guan, Luqian Zhou, Weiliang Wu, Yuqiong Yang, Xiaoying Li, Fengyan Wang, Bingpeng Guo, Zhenyu Liang, and Lin Lin
- Subjects
Adult ,Male ,medicine.medical_specialty ,Time Factors ,surface sternocleidomastoid EMG ,surface parasternal intercostal muscle EMG ,Intraclass correlation ,Diaphragm ,Diaphragmatic breathing ,neural respiratory drive ,Electromyography ,International Journal of Chronic Obstructive Pulmonary Disease ,030204 cardiovascular system & hematology ,03 medical and health sciences ,Pulmonary Disease, Chronic Obstructive ,0302 clinical medicine ,Physical medicine and rehabilitation ,Predictive Value of Tests ,Internal medicine ,Forced Expiratory Volume ,medicine ,transesophageal diaphragmatic EMG ,Humans ,surface diaphragmatic EMG ,Lung ,Original Research ,Aged ,Aged, 80 and over ,COPD ,Exercise Tolerance ,medicine.diagnostic_test ,business.industry ,Respiration ,Reproducibility of Results ,General Medicine ,Middle Aged ,medicine.disease ,medicine.anatomical_structure ,030228 respiratory system ,Control of respiration ,Parasternal line ,Predictive value of tests ,Cardiology ,Exercise Test ,Feasibility Studies ,Female ,business ,Intercostal muscle - Abstract
Weiliang Wu,1,* Lili Guan,1,* Xiaoying Li,2,* Lin Lin,1 Bingpeng Guo,1 Yuqiong Yang,1 Zhenyu Liang,1 Fengyan Wang,1 Luqian Zhou,1 Rongchang Chen1 1Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 2Department of Respiratory Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China *These authors contributed equally to this work Purpose: To evaluate the compatibility and correlation between noninvasive surface respiratory electromyography and invasive transesophageal diaphragmatic electromyography measurements as facilitating indicators of neural respiratory drive (NRD) evaluation during treadmill exercise.Patients and methods: Transesophageal diaphragmatic electromyogram activity (EMGdi,es) and surface inspiratory electromyogram (EMG) activity, including surface diaphragmatic EMG activity (EMGdi,sur), surface parasternal intercostal muscle EMG activity (EMGpara), and surface sternocleidomastoid EMG activity (EMGsc), were detected simultaneously during increasing exercise capacity in 20 stable patients with COPD. EMGdi,es, EMGdi,sur, EMGpara, and EMGsc were quantified using the root mean square (RMS) and were represented as RMSdi,es, RMSdi,sur, RMSpara, and RMSsc, respectively.Results: There was a significant association between EMGdi,es and EMGdi,sur (r=0.966, p
- Published
- 2017
47. Effects of two types of equal-intensity inspiratory muscle training in stable patients with chronic obstructive pulmonary disease: A randomised controlled trial
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Xiaoying Li, Rongchang Chen, Lili Guan, Xianming Zhang, Weiliang Wu, Luqian Zhou, Lin Lin, Bingpeng Guo, Yuqiong Yang, and Yonger Ou
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Male ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Maximal Respiratory Pressures ,Health Status ,Vital Capacity ,Pulmonary disease ,Breathing Exercises ,law.invention ,Pulmonary Disease, Chronic Obstructive ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,Quality of life ,law ,Forced Expiratory Volume ,Humans ,Medicine ,Muscle Strength ,030212 general & internal medicine ,Aged ,COPD ,Exercise Tolerance ,business.industry ,Confounding ,Inspiratory muscle training ,Middle Aged ,Exercise capacity ,medicine.disease ,Respiratory Muscles ,Intensity (physics) ,Dyspnea ,Treatment Outcome ,Inhalation ,030228 respiratory system ,Anesthesia ,Quality of Life ,cardiovascular system ,Physical therapy ,Female ,business - Abstract
We conducted a randomised controlled trial to assess the effects of daily breathing pattern changes to stable patients with COPD excluding the confounding factors of inspiratory muscle mobilization, by ensuring the load intensities of two inspiratory training devices were equal.Sixty patients with COPD were randomised to three groups: resistive-IMT group (T-IMT, 21 patients), threshold-IMT (R-IMT, 19 patients), and a control group (20 patients). Inspiratory load intensity for both methods was set at 60% of maximal inspiratory pressure (MIP), a measure of inspiratory muscle strength, which, along with health-related quality of life (HRQoL), degree of dyspnoea, and exercise capacity, were conducted before and after 8 weeks of daily IMT.At 8 weeks, there was no significantly difference of MIP between the R- and T-IMT groups (P 0.05). Chronic Respiratory Disease Questionnaire and Transition Dyspnea Index scores improved significantly after each training program compared with controls (P 0.05), and R-IMT was significant better (P 0.05). R-IMT was better than T-IMT in performance of exercise (P 0.05).In summary, in clinically stable patients with COPD, 8 weeks of R-IMT was superior to 8 weeks of equal-intensity T-IMT in improving HRQoL, degree of dyspnoea, and exercise capacity.
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- 2017
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48. Preparation of ZnS thin films and ZnS/p-Si heterojunction solar cells
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Depeng Qiu, Weiliang Wu, Lun Cai, Zongcun Liang, Hui Shen, Kaifu Qiu, and Shenghao Li
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Materials science ,Analytical chemistry ,02 engineering and technology ,01 natural sciences ,law.invention ,chemistry.chemical_compound ,X-ray photoelectron spectroscopy ,law ,0103 physical sciences ,Solar cell ,General Materials Science ,Thin film ,010302 applied physics ,business.industry ,Mechanical Engineering ,Doping ,Energy conversion efficiency ,Heterojunction ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Zinc sulfide ,Semiconductor ,chemistry ,Mechanics of Materials ,Optoelectronics ,0210 nano-technology ,business - Abstract
The morphology, optical and composition properties of Zinc sulfide (ZnS) thin films prepared by thermal evaporation method were investigated. The as-deposited ZnS thin films exhibited good smoothness and showed (1 1 1) preferential orientation. The atomic concentrations of Zn = 49.98% and S = 50.02% were calculated from the XPS spectrum. Band-gap of 3.72 eV was deduced from the optical transmittance spectra. The power conversion efficiency (PCE) of the hydrogen doped AZO (HAZO)/ZnS/textured p-Si heterojunction solar cells was η = 8.83%, which is the best so far to our knowledge. The solar cell without encapsulation exhibited no degradation for more than 2400 h, indicating the good stability of the device.
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- 2017
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49. Home noninvasive positive pressure ventilation with built-in software in stable hypercapnic COPD: a short-term prospective, multicenter, randomized, controlled trial
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Rongchang Chen, Xiaoying Li, Lili Guan, Yuanlin Song, Weiliang Wu, Jianhua Chen, Bingpeng Guo, Yating Huo, Zi-Qing Zhou, Xin Chen, Zhou Yuqi, Zhenyu Liang, Jie Tan, and Luqian Zhou
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Male ,Pediatrics ,Time Factors ,Pulmonary function testing ,law.invention ,Hypercapnia ,Positive-Pressure Respiration ,Pulmonary Disease, Chronic Obstructive ,0302 clinical medicine ,Randomized controlled trial ,law ,Prospective Studies ,030212 general & internal medicine ,Prospective cohort study ,Lung ,Original Research ,COPD ,Equipment Design ,General Medicine ,Middle Aged ,Home Care Services ,Intention to Treat Analysis ,Treatment Outcome ,Anesthesia ,Female ,medicine.symptom ,China ,medicine.medical_specialty ,Randomization ,International Journal of Chronic Obstructive Pulmonary Disease ,03 medical and health sciences ,medicine ,Humans ,chronic respiratory failure ,built-in software ,Positive pressure ventilation ,Aged ,Noninvasive Ventilation ,Ventilators, Mechanical ,Intention-to-treat analysis ,business.industry ,Carbon Dioxide ,medicine.disease ,long-term oxygen therapy ,030228 respiratory system ,Therapy, Computer-Assisted ,noninvasive positive pressure ventilation ,business ,Biomarkers ,Software - Abstract
Luqian Zhou,1,* Xiaoying Li,1,* Lili Guan,1,* Jianhua Chen,1 Bingpeng Guo,1 Weiliang Wu,1 Yating Huo,1 Ziqing Zhou,1 Zhenyu Liang,1 Yuqi Zhou,2 Jie Tan,3 Xin Chen,4 Yuanlin Song,5 Rongchang Chen1 1Department of Respiratory Medicine, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 2Department of Respiratory Medicine, The Third Affiliated Hospital of Sun-Yat Sen University, 3Department of Respiratory Medicine, The First Affiliated Hospital, School of Clinical Medicine of Guangzhou Pharmaceutical University, 4Department of Respiratory Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, 5Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China *These authors contributed equally to this work Background: The benefits of noninvasive positive pressure ventilation (NPPV) in patients with hypercapnic COPD are controversial. It is presumed that methodology and appropriate use of NIV ventilator might be crucial for the outcomes. With the new built-in software, the performance of NIV can be monitored at home, which can guarantee the compliance and appropriate use. This study investigated effects of home use of NIV in hypercapnia in COPD patients using the NIV ventilator with built-in software for monitoring. Methods: The current multicenter prospective, randomized, controlled trial enrolled patients with stable GOLD stages III and IV hypercapnic COPD. Patients were randomly assigned via a computer-generated randomization sequence, with a block size of four patients, to continue optimized treatment (control group) or to receive additional NPPV (intervention group) for 3months. The primary outcome was arterial carbon dioxide pressure (PaCO2). Data were derived from built-in software and analyzed every 4weeks. Analysis was carried out with the intention to treat. This study is registered with ClinicalTrials.gov, number NCT02499718. Results: Patients were recruited from 20 respiratory units in China from October 1, 2015, and recruitment was terminated with a record of the vital statistics on May 31, 2016. A total of 115 patients were randomly assigned to the NPPV group (n=57) or the control group (n=58). Patients complied well with NPPV therapy (mean [± standard deviation] day use 5.6±1.4h). The mean estimation of leaks was 37.99±13.71L/min. The changes in PaCO2 (-10.41±0.97 vs -4.32±0.68mmHg, P=0.03) and 6-min walk distance (6MWD) (38.2% vs 18.2%,P=0.02) were statistically significant in the NPPV group versus the control group. COPD assessment test (CAT) showed a positive trend (P=0.06) in favor of the NPPV group. Pulmonary function and dyspnea were not different between groups. Conclusion: Ventilators equipped with built-in software provided methodology for monitoring NIV use at home, which could facilitate the improvement of compliance and quality control of NIV use. It was shown that three months use of NIV at home could reduce the PaCO2 and improve exercise tolerance (6MWD) in chronic hypercapnic COPD patients. Keywords: COPD, noninvasive positive pressure ventilation, long-term oxygen therapy, chronic respiratory failure, built-in software
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- 2017
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50. Dopant-free multilayer back contact silicon solar cells employing V2Ox/metal/V2Ox as an emitter
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Hui Shen, Yifeng Chen, Zongtao Liu, Bao Jie, Weiliang Wu, Kaifu Qiu, Wenjie Lin, and Binhui Liu
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010302 applied physics ,Materials science ,Fabrication ,Silicon ,Dopant ,business.industry ,General Chemical Engineering ,chemistry.chemical_element ,Nanotechnology ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Evaporation (deposition) ,law.invention ,chemistry ,law ,0103 physical sciences ,Solar cell ,Optoelectronics ,Charge carrier ,Work function ,0210 nano-technology ,business ,Common emitter - Abstract
In this study, we present novel multilayer back contact (MLBC) solar cells employing V2Ox (8 nm)/metal/V2Ox (8 nm) (VMV) multilayers as dopant-free hole-selective contacts deposited using a thermal evaporation process at low temperature. The optimized V2Ox films have a high work function and reduced O-deficiency in-gap state energy owing to the introduction of a carefully controlled O2 partial pressure during the evaporation process. The contact resistivities of VMV (12 nm Ag) and VMV (4 nm Au) contacts with n-Si are 1.58 mΩ cm2 and 0.04 mΩ cm2, respectively, which are less than that of a 16 nm V2Ox/n-Si contact. Interestingly, VMV (Au)/n-Si MLBC solar cells demonstrate improved charge carrier transport, leading to an induced p–n junction. Moreover, the dominant interfacial charge carrier transport properties of MLBC solar cells with VMV (Ag)/n-Si and VMV (Au)/n-Si contacts correspond with the diffusion–recombination model, whereas, those of MLBC solar cells with V2Ox/n-Si and VMV (Ca)/n-Si contacts correspond with the multi-tunneling capture emission model at a high-forward-bias voltage. The use of VMV (4 nm Au) as an emitter achieves an efficiency of 19.02% for this type of MLBC solar cell, which is greater than that of V2Ox/n-Si solar cells (17.58%). This work has important implications for enabling the fabrication of low-performance dopant-free back contact solar cells with high stability using a simple fabrication process.
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- 2017
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