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34 results on '"Weingartl, Hana M."'

6. Treatment with Ad5-Porcine Interferon-α Attenuates Ebolavirus Disease in Pigs

Author

Senthilkumaran, Chandrika, primary, Kroeker, Andrea L., additional, Smith, Gregory, additional, Embury-Hyatt, Carissa, additional, Collignon, Brad, additional, Ramirez-Medina, Elizabeth, additional, Azzinaro, Paul A., additional, Pickering, Bradley S., additional, Diaz-San Segundo, Fayna, additional, Weingartl, Hana M., additional, and de los Santos, Teresa, additional

Published
2022
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10. Susceptibility of pigs and chickens to SARS coronavirus

Author

Weingartl, Hana M., Copps, John, Drebot, Michael A., Marszal, Peter, Smith, Greg, Gren, Jason, Andonova, Maya, Pasick, John, Kitching, Paul, and Czub, Markus

Abstract

An outbreak of severe acute respiratory syndrome (SARS) in humans, associated with a new coronavirus, was reported in Southeast Asia, Europe, and North America in early 2003. To address speculations [...]

Published
2004

12. Paramyxoviruses

Author

Kirkland, Peter D., primary, Stephano, Alberto, additional, and Weingartl, Hana M., additional

Published
2019
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14. Evidence of exposure to henipaviruses in domestic pigs in Uganda

Author

Atherstone, Christine, primary, Diederich, Sandra, additional, Weingartl, Hana M., additional, Fischer, Kerstin, additional, Balkema‐Buschmann, Anne, additional, Grace, Delia, additional, Alonso, Silvia, additional, Dhand, Navneet K., additional, Ward, Michael P., additional, and Mor, Siobhan M., additional

Published
2019
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18. Hendra and Nipah viruses: pathogenesis, animal models and recent breakthroughs in vaccination

Author

Weingartl,Hana M and Weingartl,Hana M

Abstract

Hana M Weingartl National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada Abstract: Hendra and Nipah viruses are two highly pathogenic zoonotic members of the genus Henipavirus, family Paramyxoviridae, requiring work under biosafety level 4 conditions due to a lack of effective therapy and human vaccines. Several vaccine candidates were protective in animal models: recombinant vaccinia virus expressing Nipah virus (NiV) F and G proteins in hamsters against NiV; recombinant ALVAC–NiV F and G in swine against NiV; recombinant Hendra virus (HeV) soluble G protein (sGHeV) against HeV and NiV in cats, ferrets, horses, and African green monkeys (AGM); recombinant vesicular stomatitis virus-based vectors expressing NiV F or G against NiV in hamsters and ferrets; measles virus-based NiV G vaccine candidate in hamsters and AGMs against NiV; and adenoassociated virus expressing NiG protein, which protected hamsters against NiV. The sGHeV was licensed for use in horses (Equivac HeV®) in 2012. It is the first vaccine candidate licensed against a biosafety level 4 agent. With the development of suitable animal models (ferret, hamster and, importantly, AGM), progress can be made toward development of a human vaccine.Keywords: henipavirus, equine, swine, human infection, animal models, vaccine candidates

Published
2015

23. Genetic and pathobiologic characterization of pandemic H1N1 2009 influenza viruses from a naturally infected swine herd

Author

Weingartl, Hana M., Berhane, Yohannes, Hisanaga, Tamiko, Neufeld, James, Kehler, Helen, Emburry-Hyatt, Carissa, Hooper-McGreevy, Kathleen, Kasloff, Samantha, Dalman, Brett, Bystrom, Jan, Alexandersen, Soren, Li, Yan, Pasick, John, Weingartl, Hana M., Berhane, Yohannes, Hisanaga, Tamiko, Neufeld, James, Kehler, Helen, Emburry-Hyatt, Carissa, Hooper-McGreevy, Kathleen, Kasloff, Samantha, Dalman, Brett, Bystrom, Jan, Alexandersen, Soren, Li, Yan, and Pasick, John

Published
2010

28. Genetic and Pathobiologic Characterization of Pandemic H1N1 2009 Influenza Viruses from a Naturally Infected Swine Herd

Author

Weingartl, Hana M., primary, Berhane, Yohannes, additional, Hisanaga, Tamiko, additional, Neufeld, James, additional, Kehler, Helen, additional, Emburry-Hyatt, Carissa, additional, Hooper-McGreevy, Kathleen, additional, Kasloff, Samantha, additional, Dalman, Brett, additional, Bystrom, Jan, additional, Alexandersen, Soren, additional, Li, Yan, additional, and Pasick, John, additional

Published
2010
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30. Experimental Infection of Pigs with the Human 1918 Pandemic Influenza Virus

Author

Weingartl, Hana M., primary, Albrecht, Randy A., additional, Lager, Kelly M., additional, Babiuk, Shawn, additional, Marszal, Peter, additional, Neufeld, James, additional, Embury-Hyatt, Carissa, additional, Lekcharoensuk, Porntippa, additional, Tumpey, Terrence M., additional, García-Sastre, Adolfo, additional, and Richt, Jürgen A., additional

Published
2009
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32. Immunopathogenesis of Severe Acute Respiratory Disease in Zaire ebolavirus-Infected Pigs.

Author

Nfon, Charles K., Leung, Anders, Smith, Greg, Embury-Hyatt, Carissa, Kobinger, Gary, and Weingartl, Hana M.

Subjects
SARS disease, EBOLA virus, VIRUS diseases in swine, RNA viruses, HEMORRHAGE, DISEASE susceptibility, DNA microarrays
Abstract

Ebola viruses (EBOV) are filamentous single-stranded RNA viruses of the family Filoviridae. Zaire ebolavirus (ZEBOV) causes severe haemorrhagic fever in humans, great apes and non-human primates (NHPs) with high fatality rates. In contrast, Reston ebolavirus (REBOV), the only species found outside Africa, is lethal to some NHPs but has never been linked to clinical disease in humans despite documented exposure. REBOV was isolated from pigs in the Philippines and subsequent experiments confirmed the susceptibility of pigs to both REBOV and ZEBOV with predilection for the lungs. However, only ZEBOV caused severe lung pathology in 5–6 weeks old pigs leading to respiratory distress. To further elucidate the mechanisms for lung pathology, microarray analysis of changes in gene expression was performed on lung tissue from ZEBOV-infected pigs. Furthermore, systemic effects were monitored by looking at changes in peripheral blood leukocyte subsets and systemic cytokine responses. Following oro-nasal challenge, ZEBOV replicated mainly in the respiratory tract, causing severe inflammation of the lungs and consequently rapid and difficult breathing. Neutrophils and macrophages infiltrated the lungs but only the latter were positive for ZEBOV antigen. Genes for proinflammatory cytokines, chemokines and acute phase proteins, known to attract immune cells to sites of infection, were upregulated in the lungs, causing the heavy influx of cells into this site. Systemic effects included a decline in the proportion of monocyte/dendritic and B cells and a mild proinflammatory cytokine response. Serum IgM was detected on day 5 and 6 post infection. In conclusion, a dysregulation/over-activation of the pulmonary proinflammatory response may play a crucial role in the pathogenesis of ZEBOV infection in 5–6 weeks old pigs by attracting inflammatory cells to the lungs. [ABSTRACT FROM AUTHOR]

Published
2013
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33. PB1-F2 Attenuates Virulence of Highly Pathogenic Avian H5N1 Influenza Virus in Chickens

Author

Bernard Delmas, Christophe Chevalier, Olivier Leymarie, Luc Jouneau, Ronan Le Goffic, Marco Moroldo, Hana M. Weingartl, Yohannes Berhane, Carissa Embury-Hyatt, Bruno Da Costa, Weingartl, Hana M., Le Goffic, Ronan, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), National Centre for Foreign Animal Disease, Canadian Food Inspection Agency (CFIA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Medical Microbiology, University of Manitoba [Winnipeg], Conseil regional d'Ile-de-France through DIM Malinf ('Domaine d'Interet Majeur Maladies Infectieuses, parasitaires et nosocomiales emergentes') [dim100157], OECD Co-operative Research Programme [TAD/PROG 67049], 'DIM Malinf' doctoral fellowships - 'Conseil regional d'Ile-de-France', Unité de recherche Virologie et Immunologie Moléculaires (VIM), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], viruses, lcsh:Medicine, Chick Embryo, Pathogenesis, Virus Replication, Pathology and Laboratory Medicine, medicine.disease_cause, Mice, Emerging Viral Diseases, Zoonoses, Medicine and Health Sciences, Influenza A virus, Cluster Analysis, lcsh:Science, Antigens, Viral, Lung, Immune Response, Avian influenza A viruses, Innate Immune System, Multidisciplinary, Virulence, virus diseases, Medical microbiology, Virus Shedding, 3. Good health, Infectious Diseases, Veterinary Diseases, Host-Pathogen Interactions, Research Article, animal structures, Immunology, Biology, Microbiology, Virus, Viral Proteins, Immune system, Orthomyxoviridae Infections, Virology, medicine, Animals, Influenza viruses, Viral shedding, Inflammation, Influenza A Virus, H5N1 Subtype, Biology and life sciences, Gene Expression Profiling, lcsh:R, Immunity, Viral pathogens, Wild type, biochemical phenomena, metabolism, and nutrition, Influenza A virus subtype H5N1, Microbial pathogens, Animal Models of Infection, Gene Expression Regulation, Viral replication, Influenza in Birds, Immune System, Mutation, lcsh:Q, Veterinary Science, Chickens, Orthomyxoviruses
Abstract

Highly pathogenic avian influenza virus (HPAIV) is a permanent threat due to its capacity to cross species barriers and generate severe infections and high mortality in humans. Recent findings have highlighted the potential role of PB1-F2, a small accessory influenza protein, in the pathogenesis process mediated by HPAIV in mammals. In this study, using a recombinant H5N1 HPAIV (wt) and its PB1-F2-deleted mutant (Delta F2), we studied the effects of PB1-F2 in a chicken model. Unexpectedly, when using low inoculation dose we observed that the wt-infected chickens had a higher survival rate than the Delta F2-infected chickens, a feature that contrasts with what is usually observed in mammals. High inoculation dose had similar mortality rate for both viruses, and comparison of the bio-distribution of the two viruses indicated that the expression of PB1-F2 allows a better spreading of the virus within chicken embryos. Transcriptomic profiles of lungs and blood cells were characterized at two days post-infection in chickens inoculated with the wild type (wt) or the Delta F2 mutant viruses. In lungs, the expression of PB1-F2 during the infection induced pathways related to calcium signaling and repressed a large panel of immunological functions. In blood cells, PB1-F2 was associated with a gene signature specific for mitochondrial dysfunction and down-modulated leucocytes activation. Finally we compared the effect of PB1-F2 in lungs of chickens and mice. We identified that gene signature associated to tissue damages is a PB1-F2 feature shared by the two species; by contrast, the early inhibition of immune response mediated by PB1-F2 observed in chickens is not seen in mice. In summary, our data suggest that PB1-F2 expression deeply affect the immune response in chickens in a way that may attenuate pathogenicity at low infection dose, a feature differing from what was previously observed in mammal species.

Published
2014

34. Comparison of assays for the detection of West Nile virus antibodies in chicken serum.

Author

Weingartl HM, Drebot MA, Hubálek Z, Halouzka J, Andonova M, Dibernardo A, Cottam-Birt C, Larence J, and Marszal P

Subjects
Animals, Chickens, Enzyme-Linked Immunosorbent Assay methods, Female, Hemagglutination Inhibition Tests methods, Immunoglobulin G blood, Immunoglobulin M blood, Neutralization Tests methods, Poultry Diseases blood, Poultry Diseases diagnosis, Poultry Diseases immunology, Sensitivity and Specificity, Specific Pathogen-Free Organisms, West Nile Fever blood, West Nile Fever diagnosis, West Nile Fever immunology, West Nile Fever veterinary, Antibodies, Viral blood, Enzyme-Linked Immunosorbent Assay veterinary, Hemagglutination Inhibition Tests veterinary, Neutralization Tests veterinary, West Nile virus immunology
Abstract

Six tests for the detection of West Nile virus (WNV) antibodies in the serum of experimentally infected chickens were compared. The tests included the hemagglutination-inhibition test (HIT), immunoglobulin M (IgM)-capture enzyme-linked immunosorbent assay (ELISA) with WNV-infected mouse brain antigen, immunoglobulin G (IgG) indirect ELISA with tickborne encephalitis viral antigen, the microtitre virus neutralization test, the standard plaque reduction neutralization test (PRNT), and the microtitre PRNT (micro-PRNT). Thirty adult chickens, intravenously and intramuscularly inoculated with 10(7) plaque-forming units (PFU) of WNV strain Egypt 101, were bled and given a booster of 10(7) PFU at 7,15, and 21 d postinoculation; the final blood collection was on day 28. Although the micro-PRNT is capable of detecting the highest antibody titres during both early and late infection, because of the technical complexity and time requirements of this test a combination of IgM and IgG ELISAs is recommended for serologic screening. Serum samples that give positive results in the ELISAs can then be tested by the micro-PRNT to determine the specificity of antibodies to WNV.

Published
2003

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