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Abstract Background Tumor-associated macrophages (TAM) are critical elements of intercellular communication in tumor microenvironment (TME), and exosomes are key mediators between tumor cells and the TME. According to previous reports, miRNAs exert a pivotal role in ovarian cancer (OC) development. The purpose of this work was to explore the function of TAM-derived exosomal miR-589-3p in OC development and elucidate the underlying molecular mechanisms. Methods First, peripheral blood mononuclear cells (PBMC) were treated with IL-4 and IL-13 to polarize them into M2-type macrophages. Exosomes were separated from M2-type macrophages, and the physical properties of exosomes were evaluated using transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). Next, quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was applied to examine the expression of relevant genes. Subsequently, Targetscan and miRDB were utilized to predict miR-589-3p target genes, and then the interaction between miR-589-3p and BCL2L13 was verified by dual luciferase assay and RNA Binding Protein Immunoprecipitation (RIP) assay. Finally, Cell Counting Kit-8 (CCK-8) and flow cytometry experiments were employed to explore the changes in the proliferative and apoptotic abilities of OC cells. Results In this research, we demonstrated that TAM-derived exosomes facilitated OC cell proliferation and suppressed OC cell apoptosis. Then, qRT-PCR results indicated that miR-589-3p were markedly elevated after co-culture of TAM-derived exosomes with OC cells. In addition, we discovered that miR-589-3p was bound to BCL-2-like protein 13 (BCL2L13), which was confirmed through luciferase assay and RIP assay. Furthermore, functional analysis displayed that TAM-derived exosomes treated with miR-589-3p inhibitor attenuated the promotion of OC cell progression by exosomes. Conclusion TAM-derived exosomal miR-589-3p enhanced OC progression through BCL2L13, which offers a novel for OC therapy.

Abstract Background T1 mapping can quantify the longitudinal relaxation time of tissues. This study aimed to investigate the repeatability and reproducibility of T1 mapping radiomics features of lung cancer and the feasibility of T1 mapping-based radiomics model to predict its pathological types. Methods The chest T1 mapping images and clinical characteristics of 112 lung cancer patients (54 with adenocarcinoma and 58 with other types of lung cancer) were collected retrospectively. 54 patients underwent twice short-term T1 mapping scans. Regions of interest were manually delineated on T1 mapping pseudo-color images to measure the mean native T1 values of lung cancer, and radiomics features were extracted using the semi-automatic segmentation method by two independent observers. The patients were randomly divided into training group (77 cases) and validation group (35 cases) with the ratio of 7:3. Interclass correlation coefficients (ICCs), Student’s t-test or Mann-Whitney U tests and least absolute shrinkage and selection operator (LASSO) were used for feature selection. The optimum features were selected to establish a logistic regression (LR) radiomics model. Independent sample t-test, Mann Whitney U-test or chi square test were used to compare the differences of clinical characteristics and T1 values. Performance was compared by the area under the receiver operating characteristic (ROC) curve (AUC). Results In the training group, smoking history, lesion type and native T1 values were different between adenocarcinoma and non-adenocarcinoma patients (P = 0.004–0.038). There were 1035 (54.30%) radiomics features meet the intra-and inter-observer, and test-retest reproducibility with ICC > 0.80. After feature dimensionality reduction and model construction, the AUC of T1 mapping-based radiomics model for predicting the pathological types of lung cancer was 0.833 and 0.843, respectively, in the training and validation cohorts. The AUCs of T1 value and clinical model (including smoking history and lesion type) were 0.657 and 0.692 in the training group, and 0.722 and 0.686 in the validation group. Combined with T1 mapping radiomics, clinical model and T1 value to establish a combined model, the prediction efficiency was further improved to 0.895 and 0.915 in the training and validation groups. Conclusions About 50% of the T1 mapping-based radiomics features displayed relatively poor repeatability and reproducibility. While T1 mapping-based radiomics model is valuable in identification of histological types of lung cancer despite the measurement variability. Combination of T1 mapping radiomics model, clinical characteristics and native T1 value can improve the predictive value of pathological types of lung cancer.

The Jiamusi section of the Songhua River is one of the first 17 model river construction sections in China. The implementation of river health assessments can determine the health dynamics of rivers and test the management's effectiveness. Targeting seven rivers, this study conducted river zoning and monitoring point deployment to conduct sufficient field research and monitoring. The authors selected hydrological and water resources, physical structure, water quality, aquatic life, social service functions, and management as guideline layers and 15 indicator layers. Subsequently, the authors established an evaluation index system to evaluate and analyze the ecological status and social service status of each river. The results showed that the Yindamu, Alingda, and Gejie rivers scored well as healthy rivers, with health evaluation scores of 78.98, 76.06, and 75.83, respectively. The Wangsanwu, Lujiagang, and Lingdangmai rivers are generally sub-healthy rivers with scores of 71.55, 67.97, and 60.7, respectively. The Yinggetu River has a score of 54.52 and is therefore assessed as unhealthy. Based on the scientific evaluation index method, this study analyses the current river health state in Jiamusi City to provide the basis for the evaluation of the river chief's work and future river management. HIGHLIGHTS Established a river health evaluation system using a graded index scoring method.; Assessed the health status of seven rivers in Jiamusi City.; Some measures and suggestions for the future management of water resources are given.;

This study systematically investigated the stable configurations and oxygen reduction reaction (ORR) catalytic activity of PN co-doped graphene using first-principles methods. We found that PN co-doped graphene substrates are generally highly stable. The adsorption energy of adsorbates is linearly positively correlated with the number of electrons obtained from the substrate. The P atoms serve as catalytic activity sites, the co-doping of N significantly enhances the adsorption energies of intermediate species in the ORR process, facilitating the direct dissociation of O2 and O2H. The solvation effect has a non-negligible impact on the adsorption energy of adsorbates, especially for O2. Due to the excessive adsorption of O, it poisons and inhibits the catalytic activity of P active sites for ORR. However, after O adsorption, the C atoms neighboring the PN impurity atoms in the P-Nn-Gra (n=2,3) substrates exhibit better catalytic activity than that of graphene doped with P/N alone. The P-Nn-defect-Gra (n=2,3,4) substrates are potential catalysts with good HER catalytic activity.

The hydrological cycle is altered by climate change and human activities, amplifying extreme precipitation and heightening the flood risk regionally and globally. It is imperative to explore the future possible alterations in flood risk at the regional scale. Focusing on the Hanjiang river basin (HRB), this study develops a framework for establishing a scientific assessment of spatio-temporal dynamics of future flood risks under multiple future scenarios. In this framework, a GCMs statistical downscaling method based on machine learning is used to project future precipitation, the PLUS model is used to project future land use, the digitwining watershed model (DWM) is used to project future runoff, and the entropy weight method is used to calculate risk. Six extreme precipitation indices are calculated to project the spatio-temporal patterns of future precipitation extremes in the HRB. The results of this study show that the intensity (Rx1day, Rx5day, PRCPTOT, SDII), frequency (R20m), and duration (CWD) of future precipitation extremes will be consistently increasing over the HRB during the 21st century. The high values of extreme precipitation indices in the HRB are primarily located in the southeast and southwest. The future annual average runoff in the upper HRB during the near-term (2023–2042) and mid-term (2043–2062) is projected to decrease in comparison to the baseline period (1995–2014), with the exception of that during the mid-term under the SSP5-8.5 scenario. The high flood risk center in the future will be distributed in the southwestern region of the upper HRB. The proportions of areas with high and medium–high flood risk in the upper HRB will increase significantly. Under the SSP5-8.5 scenario, the area percentage with high flood risk during the future mid-term will reach 24.02%. The findings of this study will facilitate local governments in formulating effective strategic plans for future flood control management.

Urban water dissipation is a significant part of the urban hydrologic cycle and has a typical natural–social dualistic attribute. Besides natural evaporation, the water dissipation in people's daily life and production process cannot be ignored. This study developed an urban water dissipation model based on different land uses and applied it in urban-built areas in Beijing. The results showed that the water dissipation of buildings and green spaces occupied the dominant position, and the water dissipation intensity of each district exceeded 500 mm, among which the six core districts were 700–1,100 mm. Comparing the water dissipation contribution rate and area rate of each underlying surface, it showed that the water dissipation intensity from strong to weak was building, water surface, green spaces, and hardened ground. According to the dualistic analysis of urban water dissipation, the contribution rates of social water dissipation in the six core districts were 45.3–69.1%, which was higher than the 17.8–36.1% of other suburbs obviously. This study reflected that the higher the degree of regional urbanization, the greater the water dissipation intensity, and artificial water dissipation was the main influencing factor. HIGHLIGHTS Developed a city-scale water dissipation calculation model based on urban land types.; Urban water dissipation includes traditional natural evapotranspiration and artificial water dissipation.; Buildings and green spaces are the major part of water dissipation in urban areas.; Regions with a high degree of urbanization in urban areas have greater water dissipation intensity.;

Abstract Positive human epidermal growth factor receptor 2 (HER2) expression is associated with an increased risk of metastases especially those to the brain in patients with advanced breast cancer (BC). Neratinib as a tyrosine kinase inhibitor can prevent the transduction of HER1, HER2 and HER4 signaling pathways thus playing an anticancer effect. Moreover, neratinib has a certain efficacy to reverse drug resistance in patients with BC with previous HER2 monoclonal antibody or targeted drug resistance. Neratinib, as monotherapy and in combination with other therapies, has been tested in the neoadjuvant, adjuvant, and metastatic settings. Neratinib with high anticancer activity is indicated for the prolonged adjuvant treatment of HER2-positive early BC, or in combination with other drugs including trastuzumab, capecitabine, and paclitaxel for the treatment of advanced HER2-positive BC especially cancers with central nervous system (CNS) metastasis to reduce the risk of BC recurrence. This article reviewed the pharmacological profiles, efficacy, safety, tolerability, and current clinical trials pertaining to neratinib, with a particular focus on the use of neratinib in patients with metastatic breast cancer (MBC) involving the CNS. We further discussed the use of neratinib for HER2-negative and HER2-mutant breast cancers, and mechanisms of resistance to neratinib. The current evidence suggests that neratinib has promising efficacy in patients with BC which is at least non-inferior compared to previous therapeutic regimens. The most common AE was diarrhea, and the incidence, severity and duration of neratinib-related grade 3 diarrhea can be reduced with loperamide. Of note, neratinib has the potential to effectively control and prevent brain metastasis in patients with advanced BC, providing a therapeutic strategy for HER2-positive BC.

Abstract Climate change significantly impacted on the survival, development, distribution, and abundance of living organisms. The Chinese serow Capricornis milneedwardsii, known as the “four unlike,” is a Class II nationally protected species in China. In this study, we predicted the geographical suitability of C. milneedwardsii under current and future climatic conditions using MaxEnt. The model simulations resulted in area under the receiver operating characteristic curve (AUC) values above 0.9 for both current and future climate scenarios, indicating the excellent performance, high accuracy, and credibility of the MaxEnt model. The results also showed that annual precipitation (Bio12), slope, elevation, and mean temperature of wettest quarter (Bio8) were the key environmental variables affecting the distribution of C. milneedwardsii, with contributions of 31.2%, 26.4%, 11%, and 10.3%, respectively. The moderately and highly suitable habitats were mainly located in the moist area of China, with a total area of 34.56 × 104 and 16.61 × 104 km2, respectively. Under future climate change scenarios, the areas of suitability of C. milneedwardsii showed an increasing trend. The geometric center of the total suitable habitats of C. milneedwardsii would show the trend of northwest expansion and southeast contraction. These findings could provide a theoretical reference for the protection of C. milneedwardsii in the future.

With accelerated urbanization and climate change, urban flooding is becoming more and more serious. Flood risk assessment is an important task for flood management, so it is crucial to map the spatial and temporal distribution of flood risk. This paper proposed an urban flood risk assessment method that takes into account the influences of hazard, vulnerability, and exposure, by constructing a multi-index urban flood risk assessment framework based on Geographic Information System (GIS). To determine the weight values of urban flood risk index factors, we used the analytic hierarchy process (AHP). Also, we plotted the temporal and spatial distribution maps of flood risk in Zhengzhou City in 2000, 2005, 2010, 2015, and 2020. The analysis results showed that, the proportion of very high and high flood risk zone in Zhengzhou City was 1.362%, 5.270%, 4.936%, 12.151%, and 24.236% in 2000, 2005, 2010, 2015, and 2020, respectively. It is observed that the area of high flood risk zones in Zhengzhou City showed a trend of increasing and expanding, of which Dengfeng City, Xinzheng City, Xinmi City, and Zhongmu County had the fastest growth rate and the most obvious increase. The flood risk of Zhengzhou City has been expanding with the development of urbanization. The method is adapted to Zhengzhou City and will have good adaptability in other research areas, and its risk assessment results can provide a scientific reference for urban flood management personnel. In the future, the accuracy of flood risk assessment can be further improved by promoting the accuracy of basic data and reasonably determining the weight values of index factors. The risk zoning map can better reflect the risk distribution and provide a scientific basis for early warning of flood prevention and drainage.

Seismic landslide hazard assessment plays a very important guiding role during urgent earthquake relief. In August 2017, an Ms 7.0 earthquake in Jiuzhaigou County, Sichuan Province, China, triggered thousands of landslides. Based on the analysis of geological settings and coseismic landslide characteristics, the Newmark model is used to complete the seismic landslide hazard assessment. Three seismic motion parameters, namely, peak ground acceleration (PGA), traditional Arias intensity (Arias_P), and improved Arias intensity (Arias_C), are adopted. A publicly published coseismic landslide catalog is used as the validation samples. The results show that the coseismic landslides are mainly distributed in the deep gullies and steep mountainous slopes on the north and south sides of the epicenter. The seismic landslide hazard accuracy based on Arias_C is the best, followed by that based on PGA and Arias_P. The spatial distribution of seismic landslide hazards based on Arias_C shows an almost standard elliptical ring and is in good agreement with that of coseismic landslides. These results fully reflect the combined influence of the epicenter and seismogenic fault on landslide development. The middle seismic landslide hazard and over are mainly located at areas with seismic intensity of VII degree and above. The Arias intensity is very suitable for rapid seismic landslide hazard assessment in emergency situations. The study results can provide scientific and technological support for rapid earthquake relief and have reference significance for future seismic landslide hazard assessment.

Abstract Camera surveillance systems can record urban waterlogging processes. Objects with regular shapes and fixed sizes captured by the camera can be utilized to calculate urban waterlogging depths based on geometric principles. In this study, we propose a machine learning‐based method to measure urban waterlogging depths using wheels and traffic buckets captured in video images as reference objects. This method is validated through laboratory experiments and observed data. The results demonstrate that: (1) the urban waterlogging depths calculated using urban reference objects show high consistency with the observed water level data; (2) in the laboratory scenario, the probability of error within 3 cm for measurements based on the hub, tire, and traffic bucket are 99.07%, 99.38%, and 81.55%, respectively; (3) in the real‐world scenario, the probability of error within 3 cm for measurements based on car hubs and pickup truck hubs are 97.30% and 95.14%, respectively. In conclusion, urban waterlogging depths can be accurately measured using reference objects with regular shapes. The proposed method can help obtain waterlogging data with higher temporal and spatial resolution at lower economic costs, which is of great significance for urban flood control.

Large earthquakes occur frequently around the world. Due to the fragility of loess, the effects of seismic events on loess tunnel projects have received considerable in-depth research attention. In this paper, we systematically review recent seismic research on loess tunnels and highlight deficiencies that require further study with a focus on the following topics: site selection, tunneling through landslides, shallow-buried tunnels with bias pressure, dynamic analysis, and seismic liquefaction. For tunnels constructed through loess landslides, comprehensive prevention measures should be implemented to ensure the stability of the landslide and tunnel. For shallow-buried loess tunnels with bias pressure, the lining of the deep-buried side should receive more attention than that of the shallow-buried side. It is necessary to consider the combined effects of seismic activity, rainfall, and train load on the stability and fortification of loess tunnels. The stress state of the surrounding loess should also be considered in further studies on tunnel liquefaction. Finally, the laboratory planar strain method should be simplified to address the discrepancy between laboratory and field results.

Abstract Background Microsatellites are a ubiquitous occurrence in prokaryotic and eukaryotic genomes. Microsatellites have become one of the most popular classes of genetic markers due to their high reproducibility, multi-allelic nature, co-dominant mode of inheritance, abundance and wide genome coverage. We characterised microsatellites in the genomes and genes of two bat species, Pteropus vampyrus and Miniopterus natalensis. This characterisation was used for gene ontology analysis and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment of coding sequences (CDS). Results Compared to M. natalensis, the genome size of P. vampyrus is larger and contains more microsatellites, but the total diversity of both species is similar. Mononucleotide and dinucleotide repeats were the most diverse in the genome of the two species. In each bat species, the microsatellite bias was obvious. The microsatellites with the largest number of repeat motifs in P. vampyrus from mononucleotide to hexanucleotide were (A)n, (AC)n, (CAA)n, (AAAC)n, (AACAA)n and (AAACAA)n, with frequencies of 97.94%, 58.75%, 30.53%, 22.82%, 54.68% and 22.87%, respectively, while in M. natalensis were (A)n, (AC)n, (TAT)n, (TTTA)n, (AACAA)n and (GAGAGG)n, with of 92.00%, 34.08%, 40.36%, 21.83%, 25.42% and 12.79%, respectively. In both species, the diversity of microsatellites was highest in intergenic regions, followed by intronic, untranslated and exonic regions and lowest in coding regions. Location analysis indicated that microsatellites were mainly concentrated at both ends of the genes. Microsatellites in the CDS are thus subject to higher selective pressure. In the GO analysis, two unique GO terms were found only in P. vampyrus and M. natalensis, respectively. In KEGG enriched pathway, the biosynthesis of other secondary metabolites and metabolism of other amino acids in metabolism pathways were present only in M. natalensis. The combined biological process, cellular components and molecular function ontology are reflected in the GO analysis and six functional enrichments in KEGG annotation, suggesting advantageous mutations during species evolution. Conclusions Our study gives a comparative characterization of the genomes of microsatellites composition in the two bat species. And also allow further study on the effect of microsatellites on gene function as well as provide an insight into the molecular basis for species adaptation to new and changing environments.

Purpose The catheter-based ultrasound (CBUS) can reach the target tissue directly and achieve rapid treatment. The frequency shift keying (FSK) signal is proposed to regulate and evaluate tumor ablation by a miniaturized dual-frequency transducer.Methods A dual-frequency transducer prototype (3 × 7 × 0.4 mm) was designed and fabricated for the CBUS applicator (OD: 3.8 mm) based on the fundamental frequency of 5.21 MHz and the third harmonic frequency of 16.88 MHz. Then, the acoustic fields and temperature field distributions using the FSK signals (with 0, 25, 50, 75, and 100% third harmonic frequency duty ratios) were simulated by finite element analysis. Finally, tissue ablation and temperature monitoring were performed in phantom and ex vivo tissue, respectively.Results At the same input electrical power (20 W), the output acoustic power of the fundamental frequency of the transducer was 10.03 W (electroacoustic efficiencies: 50.1%), and that of the third harmonic frequency was 6.19 W (30.6%). As the third harmonic frequency duty ratios increased, the shape of thermal lesions varied from strip to droplet in simulated and phantom experimental results. The same trend was observed in ex vivo tests.Conclusion Dual-frequency transducers excited by the FSK signal can control the morphology of lesions.Significance The acoustic power deposition of CBUS was optimized to achieve precise ablation.

Abstract Projecting precipitation changes is essential for researchers to understand climate change impacts on hydrological cycle. This study projected future precipitation over the Hanjiang River Basin (HRB) based on the multimodel ensemble (ME) of six global climate models from Phase 6 of the Coupled Model Intercomparison Project (CMIP6). An ME method using the fusion of four machine learning (ML) algorithms (random forest [RF], K‐nearest neighbors [KNN], extra tree [ET], and gradient boosting decision tree [GBDT]) was proposed in this study. The future precipitation changes were investigated during 2023–2042 (Near‐term), 2043–2062 (Mid‐term), and 2081–2100 (Long‐term) periods, with reference to the base period 1995–2014, under three integrated scenarios (SSP1‐2.6, SSP2‐4.5, and SSP5‐8.5) of the Shared Socioeconomic Pathways (SSPs) and the representative concentration pathways (RCPs). The results show that: (1) the proposed ME method performs better than the ME mean and individual ML algorithms, with a correlation coefficient value reaching 0.88 and Taylor skill score reaching 0.764. (2) The precipitation under SSP5‐8.5 has the largest upward trend with the annual precipitation variation range of −9.27% to 112.84% from 2023 to 2100, followed by SSP2‐4.5 with −30.48% to 44.67%, and the smallest under SSP1‐2.6 with −37.19% to 37.78%, which show a significant trend of humidification over the HRB in the future. (3) The precipitation changes over the HRB are projected to increase over time, with the largest in the Long‐term, followed by Mid‐term, and the smallest in the Near‐term. (4) The northeastern parts of the HRB are projected to experience a large precipitation in the future, and the southeastern parts are smaller. (5) Uncertainties in the projected precipitation over the HRB still exist, which can be reduced by ME. The findings obtained in this study have important implications for hydrological policymakers to make adaptive strategies to reduce the risks of climate change.

Projecting the climate change impacts on hydrology and water resources relies on the climate scenarios simulated by general circulation models (GCMs), which requires a systematic and comprehensive assessment of the GCMs’ simulation performances at a regional scale. This study evaluates the performances of precipitation simulation over the Hanjiang River Basin (HRB) by six climate models from Phase 6 of the Coupled Model Intercomparison Project (CMIP6), the corresponding six previous models from the CMIP5, and their multi-model ensemble (MME) based on the observational data in the CN05.1. To our knowledge, this is the first preliminary study in the HRB. The Taylor diagram (including standard deviation, root-mean-square difference, and correlation coefficient) and Taylor skill score are used for the evaluation of GCMs’ precipitation simulation performances. The spatial pattern and temporal pattern over the HRB simulated by CMIP6 and CMIP5 models are compared by relative biases. The results of the Taylor diagram and skill score show that CMIP6 models don't necessarily perform better than the corresponding previous CMIP5 models in simulating precipitation over the HRB. The MME exhibits superior performance compared to that of any individual model, and the CMIP6-MME is more skillful than the CMIP5-MME. As to the spatial and temporal variation characteristics, the precipitation biases are both present in CMIP6 and CMIP5 models, and the bias of the CMIP6-MME is lower than that of the CMIP5-MME. The CMIP6 and CMIP5 models overestimate the precipitation from January to June, and simulate larger precipitation biases in the areas and seasons with less precipitation, while they are lower with more precipitation over the HRB. The findings obtained in this study could provide a scientific reference for the research of future hydrological cycle predictions over the HRB. HIGHLIGHTS The prerequisite for projecting the impacts of climate change on the hydrologic cycle at a basin scale is to better understand the model biases in GCMs.; This study evaluated the precipitation simulation performances of CMIP6 and CMIP5 models over the Hanjiang River Basin, China.; Findings obtained in this study could provide a scientific reference for the research of future hydrological cycle predictions.;

Understanding the spatiotemporal pattern of precipitation concentration is important in the water cycle under changing environments. In this study, the daily precipitation concentration index in the Yangtze River Delta in China is calculated based on the Lorenz curves obtained from the observed data of 36 meteorological stations from 1960 to 2017, and spatiotemporal pattern variations and their possible causes are investigated. The driving forces of elevation, SUNSPOT, El Niño-Antarctic Oscillation, Pacific Decade Oscillation, and Arctic Oscillation are detected with correlation and wavelet analysis. Results show that, the daily precipitation concentration index ranges from 0.55 to 0.62 during the study period, 22 of 36 stations (accounting for 61%) show increasing trends, while three stations increase significantly at the 95% significant level. Relationship analysis indicates that the daily precipitation concentration shows a slightly negative correlation with elevation, while the relationships with SUNSPOT, El Niño-Antarctic Oscillation, Pacific Decade Oscillation, and Arctic Oscillation are complicated and diverse, there are different correlations and significance levels in different years. Further analysis shows that SUNSPOT is significantly correlated with El Niño-Antarctic Oscillation, Pacific Decade Oscillation, and Arctic Oscillation, which suggests that SUNSPOT may be an important factor that drives the changes of the three large-scale atmosphere circulation factors and causes precipitation concentration changing indirectly. These results provide further understanding of precipitation variations, which is meaningful for regional flood risk management under climate change. HIGHLIGHTS Spatiotemporal variations of daily precipitation concentration in the Yangtze River Delta are investigated.; Daily precipitation concentration shows a strong spatial heterogeneity overall.; Large-scale atmospheric circulation and SUNSPOTS are possible driving forces.;

Abstract Urbanization has resulted in dry/wet island effects in built-up areas. Compared to the limited number of observational datasets, simulations can provide data with richer spatial distribution, thereby proving to be more helpful for revealing the spatial distribution of dry/wet islands. This study simulated dry/wet island effects during typical summer and winter conditions in Beijing by coupling the Artificial Water Dissipation Urban Canopy Model with the Weather Research and Forecasting model. Observations of relative humidity, absolute humidity, and temperature from weather stations in Beijing were used to verify the model. The results showed that in 2020, Beijing was prone to be a dry island during summer, with the relative humidity approximately 5–10% lower than the surrounding suburbs. The dry island effect was not obvious in winter, and Beijing tended to be a wet island. The influence of artificial water dissipation on dry/wet islands is higher in winter than in summer. By considering the water vapor from artificial water dissipation, humidity in urban areas can be simulated more accurately.

Climate change directly affects water resources by changing temperature and precipitation and the responses of inland basins on plateaus to climate change show a certain pattern. To systematically evaluate the changing facts and evolution trend of temperature and precipitation in the Qinghai Lake Basin, the Weather Research and Forecasting Model (WRF) was used to simulate the spatial distribution of temperature and precipitation in typical periods of the current year based on the observations of hydrological and meteorological stations. Based on the output results of different climate models in CMIP6, the temporal changing trends of temperature and precipitation were predicted. The results showed that precipitation and runoff significantly increased compared to the past, and the lake level first decreased and then increased. In August 2020, the temperature and precipitation near the lake were higher than those in the other areas of the basin. In the future, temperature and precipitation will increase under the influence of different forcing scenarios with the temperature change being more significant. A close combination of observations and simulations will provide quantitative spatiotemporal data and technical support for future climate change adaptability research in the Qinghai Lake Basin.

Abstract Background Radiofrequency ablation (RFA) is an effective treatment option for hepatocellular carcinoma (HCC). This study aimed to analyze the prognostic factors of HCC patients treated with RFA and to develop nomograms for outcome prediction. Methods A total of 3142 HCC patients treated with RFA were recruited, and their data were collected from the Surveillance, Epidemiology, and End Results database. Univariate and multifactor Cox analyses were performed to identify independent prognostic factors. These factors were integrated into a nomogram to predict 3- and 5-year cancer-specific survival (CSS) and overall survival (OS). Consistency indices and calibration plots were used to assess the accuracy of the nomograms in both the internal and external cohorts. Results The median follow-up periods for HCC patients treated with RFA were 27 and 29 months for OS and CSS, respectively. Marital status, age, race, histological grade of differentiation, tumor size, T stage, and serum alpha-fetoprotein levels at the time of diagnosis were identified as prognostic factors for OS and CSS. Additionally, M stage was identified as risk factors for OS. These risk factors are included in the nomogram. The calibration plots of the OS and CSS nomograms showed excellent consistency between actual survival and nomogram predictions. The bootstrap-corrected concordance indices of the OS and CSS nomograms were 0.637 (95% CI, 0.628–0.646) and 0.670 (95% 0.661–0.679), respectively. Importantly, our nomogram performed better discriminatory compared with 8th edition tumor-node-metastasis (TNM) stage system for predicting OS and CSS. Conclusions We identified prognostic factors for HCC patients treated with RFA and provided an accurate and personalized survival prediction scheme.

Abstract Changes in ecosystem water use efficiency (WUE) and evapotranspiration (ET) that are driven by vegetation change can be used as metrics to quantify ecosystem carbon and water exchange. The effect of vegetation on WUE has been explored in the past through consideration of total ET. However, interception (Ei) doesn't directly participate in ecosystem carbon and water exchange, and uncertainty of modeled Ei, resulting in great uncertainty of ET partitioning and ecosystem WUE. Here, we estimated Ei in China using Gerrits' model, replacing the algorithm for Ei in the Priestley‐Taylor Jet Propulsion Laboratory (PT‐JPL) model. The modified PT‐JPL model was then incorporated into the analytical WUE model to estimate WUE over China for 2003–2011, and the results were validated against literature data (WUELiterature). To evaluate the net effect of vegetation restoration on WUE, we simulated WUE in three ways: with vegetation change; without vegetation change; and with vegetation change, but without any Ei changes caused by vegetation changes. In the study presented here, we demonstrate the WUE simulated by the analytical WUE model is significantly correlated with the WUELiterature (R2 = 0.76, p‐value

Background: Total pancreatectomy (TP) seems to be experiencing a renaissance in recent years. In this study, we aimed to determine the long-term survival of pancreatic ductal adenocarcinoma (PDAC) patients who underwent TP by comparing with pancreaticoduodenectomy (PD), and formulate a nomogram to predict overall survival (OS) for PDAC individuals following TP.Methods: Patients who were diagnosed with PDAC and received PD (n = 5,619) or TP (n = 1,248) between 2004 and 2015 were selected from the Surveillance, Epidemiology, and End Results (SEER) database. OS and cancer-specific survival (CSS) of the PD and TP groups were compared using Kaplan-Meier method and log-rank test. Furthermore, Patients receiving TP were randomly divided into the training and validation cohorts. Univariate and multivariate Cox regression were applied to identify the independent factors affecting OS to construct the nomogram. The performance of the nomogram was measured according to concordance index (C-index), calibration plots, and decision curve analysis (DCA).Results: There were no significant differences in OS and CSS between TP and PD groups. Age, differentiation, AJCC T stage, radiotherapy, chemotherapy, and lymph node ratio (LNR) were identified as independent prognostic indicators to construct the nomogram. The C-indexes were 0.67 and 0.69 in the training and validation cohorts, while 0.59 and 0.60 of the American Joint Committee on Cancer (AJCC) tumor-node-metastasis (TNM) staging system. The calibration curves showed good uniformity between the nomogram prediction and actual observation. DCA curves indicated the nomogram was preferable to the AJCC staging system in terms of the clinical utility. A new risk stratification system was constructed which could distinguish patients with different survival risks.Conclusions: For PDAC patients following TP, the OS and CSS are similar to those who following PD. We developed a practical nomogram to predict the prognosis of PDAC patients treated with TP, which showed superiority over the conventional AJCC staging system.

Background: Lymph node (LN) metastasis is considered one of the most important risk factors affecting the prognosis of distal cholangiocarcinoma (DCC). This study aimed to demonstrate the superiority of log odds of positive lymph nodes (LODDS) compared with other LN stages, and to establish a novel prognostic nomogram to predict the cancer-specific survival (CSS) of DCC.Methods: From the Surveillance, Epidemiology and End Results (SEER) database, the data of 676 patients after DCC radical operation were screened, and patients were randomly divided into training (n = 474) and validation sets (n = 474). The prognostic evaluation performance of the LODDS and American Joint Commission on Cancer (AJCC) N stage and lymph node ratio (LNR) were compared using the Akaike information criteria, receiver operating characteristic area under the curve (AUC), and C-index. Multivariate Cox analysis was used to screen independent risk factors, and a LODDS-based nomogram prognostic staging model was established. The nomogram's precision was verified by C-index, calibration curves, and AUC, and the results were compared with those of the AJCC TNM staging system.Results:Compared with the other two stages of LN metastasis, LODDS was most effective in predicting CSS in patients with DCC. Multivariate analysis proved that LODDS, histologic grade, SEER historic stage, and tumor size were independent risk factors for DCC. The C-index of the nomogram, based on the above factors, in the validation set was 0.663. The 1-, 3-, and 5-y AUCs were 0.735, 0.679, and 0.745, respectively. Its good performance was also verified by calibration curves. In addition, the C-index and Kaplan-Meier analysis showed that the nomogram performed better than the AJCC TNM staging system.Conclusion:For postoperative patients with DCC, the LODDS stage yielded better prognostic efficiency than the AJCC N and LNR stages. Compared with the AJCC TNM staging system, the nomogram, based on the LODDS, demonstrated superior performance.

BackgroundRecent studies have shown that the systemic inflammation and nutritional indicators are prognostic for a variety of malignancies. However, only limited data have so far demonstrated their usefulness in gastrointestinal mesenchymal tumors (GIST).MethodsWe retrospectively analyzed the data of GIST patients who underwent radical surgery in Beijing hospital from October 2004 to July 2018. The area under the receiver operating characteristic curve (AUC) was used to compare several commonly used inflammatory and nutritional indicators. The indicators with largest AUC were further analysis. Optimal cut-off values of those indicators in predicting recurrence-free survival (RFS) were determined. Kaplan-Meier curve and the time-dependent receiver operating characteristic (ROC) curve were used to assess the prognostic values. We then used univariate and multivariate Cox regression analyses to identify prognostic factors that were associated with RFS.ResultsIn total, 160 patients who underwent surgery for GIST were included in the study. The median survival time was 34.5 months, with 1-, 3-, and 5-year RFS rates of 96.1%, 84.7%, and 80.8%, respectively. The inflammatory and nutritional indicators with largest AUC were Systemic immunoinflammatory Index (SII) and Geriatric Nutrition Risk Index (GNRI), reached 0.650 and 0.713, respectively. The optimal cutoff of GNRI and SII were 98.3, and 820.0, respectively. Univariate analysis showed that GNRI, SII, KI67, surgery method, tumor location, tumor size, and mitotic index were all significant prognostic indicators of RFS. After multivariate Cox analysis, independent prognostic factors for RFS in GIST included tumor location, mitotic index, tumor size, and GNRI (HR=2.802,95% CI: 1.045 to 7.515, p = 0.041). Besides, SII also tended to be associated with RFS (HR = 2.970, 95% CI: 0.946 to 9.326, p = 0.062).ConclusionsHigh GNRI is an independent prognostic factor for RFS in GIST, while SII can be considered as a prognostic factor. GNRI and SII can be used as tools to evaluate the prognosis of patients before surgery, helping doctors to better treat high-risk patients.

As the prevalence of obesity increases, so does the occurrence of obesity-related complications, such as cardiovascular and cerebrovascular diseases, diabetes, and some cancers. Increased adipose tissue is the main cause of harm in obesity. To better understand obesity and its related complications, we analyzed the mRNA expression profiles of adipose tissues from 126 patients with obesity and 275 non-obese controls. Using an integrated bioinformatics method, we explored the functions of 113 differentially expressed genes (DEGs) between them. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses revealed that upregulated DEGs were enriched in immune cell chemotaxis, complement-related cascade activation, and various inflammatory signaling pathways, while downregulated DEGs enriched in nutrient metabolism. The CIBERSORT algorithm indicated that an increase in macrophages may be the main cause of adipose tissue inflammation, while decreased γδ T cells reduce sympathetic action, leading to dysregulation of adipocyte thermogenesis. A protein-protein interaction network was constructed using the STRING database, and the top 10 hub genes were identified using the cytoHubba plug-in in Cytoscape. All were confirmed to be obesity-related using a separate dataset. In addition, we identified chemicals related to these hub genes that may contribute to obesity. In conclusion, we have successfully identified several hub genes in the development of obesity, which provide insights into the possible mechanisms controlling obesity and its related complications, as well as potential biomarkers and therapeutic targets for further research.

Precipitation is a critical factor affecting regional water cycles, water ecology, and socioeconomic development. Monthly precipitation, water vapor pressure, and temperature datasets from 613 meteorological stations across China were used to analyze the spatiotemporal evolution of urban rain island effects at the national scale during periods of slow (1960–1969) and accelerated (2010–2019) urbanization. The combined effects of artificial water dissipation and heat islands on urban precipitation were a key focus of this study. The results showed that rain island effects (0–31.6 mm/month) were primarily distributed along the southeast coast (dominated by the heat island effect) and northwest inland region (dominated by artificial water dissipation). During winter, the relative contribution of artificial water dissipation was higher in urban areas, and the rain island effect was more apparent than in the summer. Comparisons of precipitation prior to and following large-scale urbanization showed that precipitation and rain island intensity along the southeast coast and northwest inland region increased by 0–28 and 0–28.6 mm/month, respectively. These findings indicate that artificial water dissipation is an important water vapor source for urban precipitation, particularly during winter months.

In this study, a strategy was developed for in situ, non-intrusive, and quantitative measurement of the oxides of nitrogen (NO and NO2) to describe emission characteristics in gas turbines. The linear calibration-free wavelength modulation spectroscopy (LCF-WMS) approach combined with the temperature profile-fitting strategy was utilized for trace NO and NO2 concentration detection with broad spectral interference from gaseous water (H2O). Transition lines near 1308 nm, 5238 nm, and 6250 nm were selected to investigate the H2O, NO, and NO2 generated from combustion. Experiments were performed under different equivalence ratios in a combustion exhaust tube, which was heated at 450–700 K, with an effective optical length of 1.57 m. Ultra-low NOx emissions were captured by optical measurements under different equivalence ratios. The mole fractions of H2O were in agreement with the theoretical values calculated using Chemkin. Herein, the uncertainty of the TDLAS measurements and the limitation of improving the relative precision are discussed in detail. The proposed strategy proved to be a promising combustion diagnostic technique for the quantitative measurement of low-absorbance trace NO and NO2 with strong H2O interference in real combustion gases.

Abstract Background Cancer metastasis is the major reason for cancer related deaths, and the mechanism of cancer metastasis still unclear. RPLP1, a member of a group of proteins known as ribosomal proteins, is associated with tumorigenesis and primary cell immortalization and is involved in cellular transformation. However, the expression and potential function of RPLP1 in TNBC remain unclear. Methods The expression of RPLP1 in TNBC tissues and cell lines were detected with Real-Time PCR and western blotting. 81 cases of TNBC tissue samples and adjacent non-tumor tissue samples were tested by immunochemistry to determine the correlation between the RPLP1 expression and clinicopathological characteristics. In vitro, we determined the role and mechanistic pathways of RPLP1 in tumor metastasis in TNBC cell lines. Results In this study, we detected high levels of RPLP1 expression in TNBC samples and cell lines. RPLP1 is upregulated in triple-negative breast cancer (TNBC) tissues and cells, and high expression levels correlate with an increased risk of recurrence and metastasis. Furthermore, high RPLP1 expression was associated with a poor prognosis and was an independent prognostic marker for TNBC. In RPLP1-induced cancer metastasis, RPLP1 may increase cancer cell invasion, which is likely the result of its effect on the cancer cell epithelial-mesenchymal transition. Conclusions Altogether, our findings indicate RPLP1 is a poor prognostic potential biomarker and anti-metastasis candidate therapeutic target in triple-negative breast cancer.

In the context of climate change and rapid urbanization, flood disaster loss caused by extreme rainstorm events is becoming more and more serious. An accurate assessment of flood disaster loss has become a key issue. In this study, extreme rainstorm scenarios with 50- and 100-year return periods based on the Chicago rain pattern were designed. The dynamic change process of flood disaster loss was obtained by using a 1D–2D coupled model, Hazard Rating (HR) method, machine learning, and ArcPy script. The results show that under extreme rainstorm events, the direct economic loss and affected population account for about 3% of the total GDP and 16% of the total population, respectively, and built-up land is the main disaster area. In addition, the initial time and the peak time of flood disaster loss increases with an increasing flood hazard degree and decreases with the increase in the return period. The total loss increases with the increase in the return period, and the unit loss decreases with the increase in the return period. Compared with a static assessment, a dynamic assessment can better reveal the development law of flood disaster loss, which has great significance for flood risk management and the mitigation of flood disaster loss.

Evaluating global climate model (GCM) outputs is essential for accurately simulating future hydrological cycles using hydrological models. The GCM multi-model ensemble (MME) precipitation simulations of the Climate Model Intercomparison Project Phases 5 and 6 (CMIP5 and CMIP6, respectively) were spatially and temporally downscaled according to a multi-site statistical downscaling method for the Hanjiang River Basin (HRB), China. Downscaled precipitation accuracy was assessed using data collected from 14 meteorological stations in the HRB. The spatial performances, temporal performances, and seasonal variations of the downscaled CMIP5-MME and CMIP6-MME were evaluated and compared with observed data from 1970–2005. We found that the multi-site downscaling method accurately downscaled the CMIP5-MME and CMIP6-MME precipitation simulations. The downscaled precipitation of CMIP5-MME and CMIP6-MME captured the spatial pattern, temporal pattern, and seasonal variations; however, precipitation was slightly overestimated in the western and central HRB and precipitation was underestimated in the eastern HRB. The precipitation simulation ability of the downscaled CMIP6-MME relative to the downscaled CMIP5-MME improved because of reduced biases. The downscaled CMIP6-MME better simulated precipitation for most stations compared to the downscaled CMIP5-MME in all seasons except for summer. Both the downscaled CMIP5-MME and CMIP6-MME exhibit poor performance in simulating rainy days in the HRB.

Intravascular ultrasound has good prospects for clinical applications in sonothrombolysis. The catheter-based side-looking intravascular ultrasound thrombolysis (e.g., Ekosonic catheters) used in clinical studies has a high frequency (2 MHz). The lower-frequency ultrasound requires a larger-diameter transducer. In our study, we designed and manufactured a small ultrasound-based prototype catheter that can emit a lower frequency ultrasound (1.1 MHz). In order to evaluate the safety and efficacy of local low-frequency ultrasound-enhanced thrombolysis, a microbubble (MB) was introduced to augment thrombolysis effect of locally delivered low-intensity ultrasound. The results demonstrated that combination of ultrasound and MB realized higher clot lysis than urokinase-only treatment (17.0% ± 1.2% vs. 14.9% ± 2.7%) under optimal ultrasound settings of 1.1 MHz, 0.414 MPa, 4.89 W/cm2, 5% duty cycle and MB concentration of 60 μg/mL. When urokinase was added, the fibrinolysis accelerated by MB and ultrasound resulted in a further increased thrombolysis rate that was more than two times than that of urokinase alone (36.7% ± 5.5% vs. 14.9% ± 2.7%). However, a great quantity of ultrasound energy was required to achieve substantial clot lysis without MB, leading to the situation that temperature accumulated inside the clot became harmful. We suggest that MB-assisted local sonothrombolysis be considered as adjuvant therapy of thrombolytic agents.

The Chinese government attaches great importance to climate change adaptation and has issued relevant strategies and policies. Overall, China’s action to adapt to climate change remains in its infancy, and relevant research needs to be further deepened. In this paper, we study the future adaptive countermeasures of Shenzhen city in the Pearl River Delta in terms of climate change, especially urban flood risk resilience. Based on the background investigation of urban flood risk in Shenzhen, this paper calculates the annual precipitation frequency of Shenzhen from 1953 to 2020, and uses the extreme precipitation index as a quantitative indicator to analyze the changes in historical precipitation and the impact of major flood disasters in Shenzhen city in previous decades. Based on the six kinds of model data of the scenario Model Inter-comparison Project (MIP) in the sixth phase of the Coupled Model Inter-comparison Project (CMIP6), uses the Taylor diagram and MR comprehensive evaluation method to evaluate the ability of different climate models to simulate extreme precipitation in Shenzhen, and the selected models are aggregated and averaged to predict the climate change trend of Shenzhen from 2020 to 2100. The prediction results show that Shenzhen will face more severe threats from rainstorms and floods in the future. Therefore, this paper proposes a resilience strategy for the city to cope with the threat of flood in the future, including constructing a smart water management system and promoting the development of a sponge city. Moreover, to a certain extent, it is necessary to realize risk transfer by promoting a flood insurance system.

This paper presents a single piezoelectric transducer with dual-frequency characters by utilizing contour-extensional mode and thickness mode. A 2.5 MHz and 8.5 MHz frequency transducer prototype is designed by Finite element method (FEM) analyses and verified by experiment. Performance of the transducer is then evaluated through the measurement of admittance curves and pulse-echo responses. It’s demonstrated experimentally that the proposed transducer has -6dB bandwidth of 39.4% @1.0 Vpp at 2.4 MHz and 34.6% @1.1 Vpp at 8.9 MHz, respectively. The proposed dual-frequency transducer has potential applications in vivo thrombolytic therapy and ultrasound-guide of pedicle screws.